Effect of Extreme Heatwaves on the Mortality and Cellular Immune Responses of Purplish Bifurcate Mussel Mytilisepta virgata (Wiegmann, 1837) (=Septifer virgatus) in Indoor Mesocosm Experiments

Hyun-Ki Hong,Chang Wan Kim,Jeong-Hwa Kim,Nobuhisa Kajino,Kwang-Sik Choi 제주대학교 해양과학연구소 2021 해양과환경연구소 연구논문집 Vol.45 No.-

In the rocky intertidal environment, the frequency and duration of heatwaves have increased over the last decade, possibly due to global climate change. Heatwaves often result in lethal or sub-lethal disturbances in benthic animals by changing their metabolic activities. In this study, we investigated the impacts of extreme heatwave stress on the hemocyte functions of Mytilisepta virgata and subsequent mortality to gain a better understanding of the potential causes and consequences of mass mortality events in this mussel during summer. We discriminated three types of hemocytes in the hemolymph, granulocytes, hyalinocytes, and blast-like cells, using flow cytometry and revealed that granulocytes were the major hemocyte involved in cellular defensive activities, such as phagocytosis and reactive oxygen species (ROS) production. For the experiment, mussels were exposed to a 40◦C air temperature for 12 h per day over 5 days under laboratory conditions as a simulated semi-diurnal tidal cycle. Mortality began to occur within 3 days after beginning the experiment, and all mussels had died by the end of the experiment. Flow cytometry indicated that the mussels exposed to high air temperatures produced significantly more ROS than did the control mussels within 2 days after the onset of the experiment, which may have caused oxidative stress. Such high levels of ROS in the hemolymph increased DNA damage in hemocytes after 3 days of exposure and decreased the phagocytosis of hemocytes 4 days after the experiment began. The observed mortality and decline in immune capacity suggested that an extreme heat event occurring in the rocky intertidal ecosystem during summer could exert sublethal to lethal impacts on macrobenthic animals.

P177 Dermoscopic findings during the course of β -blocker treatment for infantile hemangioma

( Jin-hwa Son ),( Hyun-ju Jin ),( Hyang-suk You ),( Woo-haing Shim ),( Jeong-min Kim ),( Gun-wook Kim ),( Hoon-soo Kim ),( Byung-soo Kim ),( Moon-bum Kim ),( Hyun-chang Ko ) 대한피부과학회 2016 대한피부과학회 학술발표대회집 Vol.68 No.2

<div style="display:none">fiogf49gjkf0d</div><div style="display:none">fiogf49gjkf0d</div><div style="display:none">fiogf49gjkf0d</div><div style="display:none">fiogf49gjkf0d</div><div style="display:none">fiogf49gjkf0d</div><div style="display:none">fiogf49gjkf0d</div><div style="display:none">fiogf49gjkf0d</div> Background: Dermoscopy is a useful tool for examining morphology and structure of blood vessels in vascular skin lesions like infantile hemangioma (IH). However, dermoscopic findings are not fully elucidated in the course of β-blocker treatment for IH. Objectives: To investigate changes of dermoscopic findings during the course of β-blocker treatment for IH. Methods: Superficial or mixed-type IH patients treated with topical timolol solution or oral propranolol were enrolled in Pusan National University Hospitals (Busan and Yangsan) from November 2011 to January 2016. Clinical photograph and dermoscopic findings were recorded and assessed at the baseline, 1, 2, 3 and 6 months. Results: Total 115 patients (male: 38 and female: 77 / superficial type: 48 and mixed type: 67 / timolol: 77 and propranolol: 38) were included. Diffuse light erythema tended to be diminished but milky-red area increased gradually during the course of treatment. The patients treated with oral propranolol showed more prominent changes on each dermoscopic findings than those treated with topical timolol (p=0.023 and p=0.018, respectively). But, other findings like red-globular vessels, red-dot vessels, red-circulated vessels, red-linear vessels did not show significant changes. Conclusion: Dissolution of diffuse light erythema and increase of milky-red area were significant dermoscopic findings in accordance with clinical improvement after β -blocker therapy for IH.

저소득층 가정의 유아를 위한 중재 프로그램에 참여한 어머니의 경험

류호영(You, Ho-Young),김정화(Kim, Jeong-Hwa),박창옥(Park, Chang-Ok),이성희(Lee, Sung-Hee) 한국어린이미디어학회 2013 어린이미디어연구 Vol.12 No.3

본 연구는 저소득 가정 중재 프로그램에 참여한 어머니들의 경험의 내용과 의미를 분석함으로써, 저소득 영유아의 가정 방문 중재 프로그램이 나아가야 할 방향을 제시하고자 한다. 연구 대상은 중재 프로그램에 참여한 10명의 저소득 가정 어머니들이다. 이들의 참여 경험을 알아보기 위하여 어머니들과 2013년 4월부터 7월까지 각각 2회의 개별면담과 2회의 그룹면담을 하였다. 또한 가정에 방문하여 프로그램을 제공한 교사들을 연구 기간 동안 면담하고 활동 저널을 분석하였다. 어머니들의 프로그램 참여 경험내용과 의미를 분석한 결과는 다음과 같다. 첫째, 본 중재 프로그램의 참여 어머니들의 어려움은 다양하였다. 어머니들은 ‘항상 미안한 마음’, ‘중복된 어려움’, ‘스트레스’의 어려운 상황에 있었다. 둘째, 중재 프로그램 참여를 통해 “지지의 효과와 변화”를 경험하였다. 구체적으로 ‘놀이’, ‘그림책’, ‘배움’, ‘전문교사’, ‘집단 활동’을 통해 의미 있는 변화를 경험한 것으로 나타났다. 마지막으로 “지속의 요구와 바램”의 욕구를 표현 하였다. 긍정의 변화를 지속하고자 하는 요구로서 구체적으로 ‘프로그램 지속의 요구’, ‘어머니 상담의 필요성’ ‘부모 훈련 필요성’에 관한 내용이 제시되었다. The study aimed at proposing the directions of home-visiting family intervention program for young children by analyzing the contents and the meanings of mother’s experiences in the intervention program for the low-income families. The ten mothers of low-income families who had participated in the program were served as participants for this study. To delve into reactions to their experiences, We had interviewed them one-on-one and in groups twice, respectively, from April to July, 2013. We also interviewed the teachers those who implemented the program by visiting low-income families and analyzed their activity journal entries. The results of analysis on the participants’ experiences and their meanings are threefold. First, They expressed their sorry for the children, struggles, confusion, and stress. Second, they experienced ‘the effects of support and changes’ via the intervention program. It was observed that important changes occurred through ‘play,’ ‘picture books,’ ‘pleasure in learning,’ ‘professional teachers,’ and ‘group activities.’ Finally, they articulated their needs and desires of maintaining the intervention program. Specifically, the requests of keeping positive changes included the needs of maintaining the program, opportunities for consultation and hands-on parental training.

청간소요산으로 호전된 설통 환자 3례

김연진,양승보,김정화,이상화,조승연,박성욱,박정미,고창남,Kim, Yeon-jin,Yang, Seung-bo,Kim, Jeong-hwa,Lee, Sang-hwa,Cho, Seung-yeon,Park, Seong-uk,Park, Jung-mi,Ko, Chang-nam 대한한방내과학회 2017 大韓韓方內科學會誌 Vol.38 No.4

Objectives: This clinical study reports the effects of Cheonggansoyo-san (CSS) on three patients with glossodynia. Methods: Three patients with glossodynia were treated with CSS (i.e., an herbal medicine) and acupuncture. Numeric rating scales (NRSs) for glossodynia and dry mouth and hwa-byung questionnaire scores were evaluated, and the results from heart rate variability (HRV) evaluations were analyzed. Results: After treatment with CSS, three patients' glossodynia symptoms, such as burning sensations, tingling, and numbness of the tongue, were improved. NRS ratings for glossodynia and dry mouth and the hwa-byung questionnaire scores also improved, and HRV parameters were closer to the normal range. Conclusion: Cheonggansoyo-san could be used to treat glossodynia.

치핵의 보존적 치료

장정환,김정용,주영화 조선대학교 1994 The Medical Journal of Chosun University Vol.19 No.1

This study was undertaken to evaluate the effectiveness of the conservative treatment of hemorrhoid, especially warm sitz bath and anal antihemorrhoidal suppositories, on the basis of answering the questionnaires given the 55 hemorrhoidal patients who were performed hemorrhoidectomy at Department of surgery, Chosun University and Hak Moon Surgical Clinic from Jan, 1994 to May 1994. The results are summerized briefly as follows : 1. Except 9 cases(18%), 46 cases(82%) experienced sitz and/or suppositories, Among them, 20 cases(40%) used above both methods, 19 cases(38%) made use of only sitz bath and 7 cases (14%) use of anal suppositories. 2. The effects of sitz bath are pain relief in 14 cases(35%), decreased discharge in 9 cases(23%), relief of itching in 6 cases(15%) and decreased size of hemorrhoidal mass in 5 cases(13%). 3. The reasons of using the antihemorrhoidal suppository are prolapse(15%), bleeding(29%), pain (38%), discharge(12%) and itching(7%). The effects of antihemmorrhoidal agents are varied but excellent and good group are 16 cases(62.9%). Toxic effects of antihemorrhoidal agents were not found on this study.

백미, 흑미 첨가 케이크의 물리적 특성

장정옥,류화정 동아시아식생활학회 1998 동아시아식생활학회지 Vol.8 No.1

In this research, we added 10%, 20%, and 30% of white rice powder and colored rice powder to wheat flour, then baked cakes to appraise the quality of each cake by physical and sensory evaluation. The results are as follows: (1) The specific gravity was increased in order of rice powder-added batters and colored rice powder-added batters. No remarkable difference was found in expansion ratio of cakes except some reduction in 30% rice powder-added cake. (2) Gumminess of cake was higher in the 30% colored rice-added cake. Hardness of rice-added cakes was higher than colored rice-added ones. More adding rate of rice, higher hardness of cake. No remarkable difference was found in chewiness, springiness and cohesiveness of cakes. (3) In case of color, rice-added cakes showed more L value and less b value than colored rice-added ones. Colored rice-added cakes showed less L value more a value and less b value as adding rate became higher. (4) According to the results of sensory evaluation, no remarkable difference was found in the size of a cell and distribution of cells. Crumb and tenderness tended to be lower in rice added cakes than in colored rice added ones. Color was prefered in 30% colored rice added cakes over rice-added cakes and less added colored rice added cakes.

Vitamin D_(3)가 RAW 세포에 감염된 Mycobacterium marinum의 증식억제에 미치는 기전 연구

박정규,정샛별,이길수,김수영,송창화,박종호,조현구,조은경,김화중 충남대학교 의과대학 의학연구소 2003 충남의대잡지 Vol.30 No.1

The natural resistance-associated macrophage protein 1(Nramp1) has been proposed to directly regulate bactericidal or bacteriostatic activity of the macrophage toward pathogens or participate in macrophage activation that lead to microbial elimination in the host. The relationship between Nramp1 and nitric oxide(NO) as an antimicrobial factor has not been precisely defined to date. To devise an in vitro assay for Nramp1 function, this study introduced a wild type Nramp1^(G169) cDKA transfected RAW264.7 macrophages(A8) which bear a homozygous mutant Nramp1^(D169) allele and are permissive to replication of specific intracellular parasites. RAW264.7 and A8 macrophages did not produce NO, but vitamin D_(3)-activated-Mycobacterium marinum-infected RAW264.7 and A8 macrophages pretreated with vitamin D_(3) leaded to the increase of NO production and growth inhibition of M. marinum. Inhibition of NO production by a NO inhibitor, L-NAME, abolished the above effects. The mRNA expression of iNOS in infected macrophages with costimulated vitamin D_(3) was increased. IFN-γ activated macrophages also showed the same results with vitamin D_(3) activated macrophages. These results suggest that bactericidal or bacteriostatic activity in RAW264.7 and A8 macrophages correlated with the production of NO, although NO might not be the only factor responsible for controlling M. marinum infection. The Nrampl gene is considered to be a cofactor in the controlling the replication of M. marinum infection.

HL-60 세포주를 이용한 결핵균항원의 세포성면역반응의 분석

박정규,강윤중,김운옥,임재현,송창화,조은경,김화중 충남대학교 의학연구소 2001 충남의대잡지 Vol.28 No.2

Most persons who become infected with M. tuberculosis mount a protective immune response and remain clinically well, the only evidence of infection being development of a positive tuberculin skin test. Five to 10% develop tuberculosis disease within the first 2 years after infection (primary tuberculosis) or thereafter (reactivation tuberculosis). Acquired resistance against tuberculosis paradigmatically rests on cell-mediated immunity, with the major factors being mononuclear phagocytes and T Lymphocytes. While the former cells act as the principal effectors, the latter ones serve as the predominant inducers of protection. The usefulness of the single dose of BCG routinely given in childhood in many developing countries in preventing far more commonly occurring tuberculosis in adults is in doubt. An effective and safe vaccine against tuberculosis is sorely needed. A subunit vaccine are capable of inducing protective immunity and could have substantial advantages over BCG or other whole-bacterium vaccines. The human promyelocytic cell line HL-60 adopted characteristic macrophage-like properties, including adherence and CD14 expression after a period of continuous culture at high ambient CO_(2) concentration. When HL-60 cells were cultured with 1,25-dihydroxyvitamin D_(3) for 4 days, the cells acquired the activity to potentiate T cell proliferation by the 30 kDa or 38 kDa antigen of Mycobacterium tuberculosis H37Rv Therefore, vitamin D-treated HL-60 cells showed the function of the antigen presenting cells.

Inhibitory Effect of Clavicepitaceae on Serotonin Release out of Human Platelets and Human Platelet Aggregation

박화진,--,--,--,--,--,-- THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 2004 Journal of biomedical laboratory sciences Vol.10 No.1

We have investigated the effects of hypha-water extracts (HWE), fruit body-water extracts (FWE) and cordycepin from Cordyceps militaris on serotonin release out of human platelets and human platelet aggregation. HWE and FWE inhibited the release of [^(3)H]-serotonin from human platelet stimulated by thrombin (2 U/ml) or collagen (20 ㎍/ml) in a dose-dependent manner. Furthermore, cordycepin, a major component of Cordyceps militaris, inhibited the human platelet aggregation induced by collagen (10 ㎍/ml) in a dose-dependent manner. These results suggest that cordycepin containing in HWE and FWE may inhibit the serotonin release by suppressing the collagen-induced human platelet aggregation. Accordingly, our data demonstrate that HWE and FWE containing much cordycepin might have antithrombotic and antimigrainous functions.

서울市內一部 動物病院에서 臨床診療된 愛玩犬의 病類別 分布에 對한 醫學統計學的 考察

徐政煜,李元暢,鄭柄鉉,尹和重 건국대학교 동물자원연구센터 1999 動物資源硏究誌 Vol.20 No.-

우리 나라의 소동물 수의임상분야(獸醫臨床分野)에서 주류를 이루고 있는 애완견의 병류별 질병분포를 파약하여 수의임상반야의 기초자료로 제시하고자 시도하고 서울시내 중견 동물병원 5곳을 무작위선택방법에 의하여 선정하여 1993년도부터 1995년도 말까지 3개년간의 임상의료철을 수집, 의학통계학적 방법을 이용 분석 및 관찰한바 몇 자기 결과를 얻어 다음과 같이 적요 한다. 진료과별 진료건수의 분포를 보면, 전체진료건수 8,360건 중에서 내과질환이 53.6%를 점유하고, 외과질환은 13.01%, 비뇨생식기질환1.96%, 피부과질환 15.47%, 안과질환 2.68% 그리고 이(耳)과질환 13.28% 등으로 관찰되었다. 조사 관찰된 내과질환은 총 19종의 질병과 4,481건의 진료건수가 있었으며, 이중에서 위발증(胃發症)이 가장 많아 21.38%를 점유하고 있었고, 외과질환은 성형외과와 정형외과 질환을 포함하여 역시 총 19종의 질병과 1,088건의 진료건수가 있었는데, 이중에서 외상(外傷)이 가장 많아 19.21%, 비뇨 생식기질환은 6종류의 질병과 164건의 진료건수가 있었으며, 그 중에서 비뇨기 감염증이 가장 많아 37.0%를, 피부질환은 5종의 질병에 1,293건의 진료가 있었고, 이중에서 체외기생충의 감염이 37.74% 그리고 안이과(眼耳科)질환은 7종에 1,332건으로 외이염(外耳炎)이 가장 많아 59.49%를 점유하고 있었다. 애완견에서 빈발하는 10대 질환의 분포를 관찰하바, 위염 (11.46%), 위장염(10.28%), 외이염(9.74%), 기관기관지염(8.49%), 대장염(6.41%), 체외기생충증(5.84%), 체내기생충증(4.35%), 이내(耳內) 진드기(3.8%), 피부진균증(3.53%) 및 피지누증 (3.12%) 등의 순으로 관찰되었다. 끝으로 애완견의 전염병으로는 distemper가 44.8%, parvoviral infection 39.37%, coronaviral infection 15.83% 그리고 본조사기간중 서울시내에서의 rabies 발생건수는 전혀 없었다. The purpose of this study was to a retrospective and medical statistical observation on the clinical cases of pet-dogs by veterinary hospital in Seoul City, during the period from 1993 to 1995, which important not only in veterinary clinic but also pet animal health information system development for veterinary practitioner of Korea. The results were summarized as follows: In the clinical cases determined by the international classification of diseases, during the period of three years, 8,358 cases of morbidity of pet-dogs were shown by five veterinary hospital for small animals. Among them, the disease of internal medicine, including medicine, including digestive, respiratory, circulatory, metabolic and parasitosis were 53.6% of total cases, the surgical disease, including orthopedic and plastic surgery were 13.01%, the disease of urogenital was 1.96%, the disease of dermatology was 15.47%, the disease of ophthalmic was 2.68% and the disease of otiatric was 13.28%, respectively. The gastric crisis of 21.38% among 4,481 cases of 19 kinds on the disease of internal medicine showed the highest morbidity. The trauma of 19.21% among 1,088 cases of 19 kinds in the surgery, including orthopedic and plastic surgery, the case of urogenic infection of 37.0% among 164 cases of 6 kinds in the urogenital organe, the ectoparasitosis of 37.24% among 1,293 cases of 5 kinds in the disease of dermatology, the otitis externa of 59.49% among 1,332 cases of 7 kinds n the opthalmatic and otiatric diseases showed the highest morbidity in each part of veterinary clinics. Observation on the morbidity of the main disease of pet-dogs were gastritis (11.46%), gastroenteritis (10.28%), ectoparasitosis (9.64%) including ear-mite, otitis externa (9.47%), trachobronchitis (8.49%), enterocolitis (6.41%), endoparasitosis (4.35%), dermatophytosis (3.53%) and seborrhea (3.12%), respectively. Finally, distributive observation on the incidence case of the main infections disease in pet-dogs which that distemper, parvoviral infection and coronaviral infection were 44.80%, 39.37% and 15.83, respectively.