Aberrant expression of interleukin-10 and activation-induced cytidine deaminase in B cells from patients with Behçet's disease

Yoon, Jeong-Yun,Lee, Yeojin,Yu, Seong-Lan,Yoon, Hee-Kyung,Park, Ha-Yan,Joung, Chung-Il,Park, Seok-Rae,Kwon, Mihye,Kang, Jaeku D.A. Spandidos 2017 Biomedical reports Vol.7 No.6

<P>Despite extensive studies, the pathogenesis of Behçet's disease (BD) remains unclear. In particular, the roles of B cells in patients with BD have not been elucidated. Activation-induced cytidine deaminase (AID) is a critical enzyme for immunoglobulin (Ig) heavy chain class switching and somatic hypermutation in B cells and the abnormal expression of AID in various immune conditions has previously been studied. B10 cells, an interleukin (IL)-10-secreting subset of regulatory B cells, function to downregulate inflammation and autoimmunity. Thus, in the present study, the relevance of B cells in patients with BD was investigated. The plasma levels of IL-10 and IgA and the proportions of cluster of differentiation (CD)43<SUP>+</SUP> B cells, excluding naïve B cells, were measured in 16 patients with BD and 16 age- and sex-matched healthy controls (HCs). Additionally, the mRNA levels of IL-10 and AID were assessed in B cells from fresh peripheral blood samples of the BD patients and HCs. The plasma level of IL-10 in patients with BD did not differ significantly from that in HCs. Similarly, there was no significant difference in the plasma level of IgA, although a slight increase was observed in patients with BD compared with that in HCs. There were no differences in CD43<SUP>+</SUP>CD19<SUP>+</SUP> B cell numbers between patients with BD and HCs. However, IL-10 mRNA levels were significantly reduced (P<0.05), while AID mRNA levels were significantly increased (P<0.01) in the B cells of patients with BD compared with those in HCs. These results provide insight into the role of B cells in patients with BD.</P>

Leclercia adecarboxylata균을 이용한 암모니아 가스 제거용 탈취탑 설계를 위한 연구

배재근,김준호,정일래 서울産業大學校 2005 논문집 Vol.54 No.3

본 연구에서는 이전의 Leclercia adecarboxylata균을 이용한 암모니아 악취 가스의 제거 가능성 검토 결과를 바탕으로 현장 적용을 위하여 미생물 배양배지의 조성을 달리하여 경제적인 배지성분을 얻고자 하였다. 이전의 가능성 검토 결과 연속적으로 암모니아 악취 가스가 1,000ppm으로 주입되어도 반응기내 암모니아성 질소의 농도가 증가하지 않고, 일정 수준 계속 유지 되는 것을 알수 있었다. 우선 연속식 실험에 앞서 R1에 최적 GA배지 주입량을 결정한 실험과 증류수만을 넣은 R2에서 배지내 NH4-N의 농도가 증가하지 않고, 일정하게 유지되는 것으로 나타나 제거효율적인 측면 GA배지를 주입하는 것이 가장 바람직 한 것으로 나타났다. 2번째 실험인 R2에 증류수와 글루코스 1g/L만을 넣었다. 3번째 실험은 배지내 들어가는 약품의 비용을 줄이기 위한 실험으로 R2에 미량 K₂HPO₄ 3g/L와 글루코스 1g/L, 증류수만을 넣고 실험을 하였다. 본 연구에서는 배지내 미량의 원소를 제외한 소량의 K₂HPO₄만으로도 미생물 성장이 GA배지를 넣었을 때 보다 성장이 느리긴 하지만 성장이 이루어 지는 것을 알수 있었다. 이 결과를 바탕으로 향후 탈취탑 설계에 다른 원소를 제외하고 K2HPO4만으로 효과를 얻을 수 있어 경제적인 측면에서도 가능성을 검토할 수 있었다.

Mechanism underlying the suppressor activity of retinoic acid on IL4-induced IgE synthesis and its physiological implication

Seo, Goo-Young,Lee, Jeong-Min,Jang, Young-Saeng,Kang, Seung Goo,Yoon, Sung-il,Ko, Hyun-Jeong,Lee, Geun-Shik,Park, Seok-Rae,Nagler, Cathryn R.,Kim, Pyeung-Hyeun Elsevier 2017 Cellular immunology Vol.322 No.-

<P><B>Abstract</B></P> <P>The present study extends an earlier report that retinoic acid (RA) down-regulates IgE Ab synthesis in vitro. Here, we show the suppressive activity of RA on IgE production in vivo and its underlying mechanisms. We found that RA down-regulated IgE class switching recombination (CSR) mainly through RA receptor α (RARα). Additionally, RA inhibited histone acetylation of germ-line ε (GL ε) promoter, leading to suppression of IgE CSR. Consistently, serum IgE levels were substantially elevated in vitamin A-deficient (VAD) mice and this was more dramatic in VAD-lecithin:retinol acyltransferase deficient (LRAT<SUP>−/−</SUP>) mice. Further, serum mouse mast cell protease-1 (mMCP-1) level was elevated while frequency of intestinal regulatory T cells (Tregs) were diminished in VAD LRAT<SUP>−/−</SUP> mice, reflecting that deprivation of RA leads to allergic immune response. Taken together, our results reveal that RA has an IgE-repressive activity in vivo, which may ameliorate IgE-mediated allergic disease.</P> <P><B>Highlights</B></P> <P> <UL> <LI> RA represses IgE CSR through RA receptor alpha (RARα). </LI> <LI> RA inhibits histone acetylation of Ig germ-line ε (GL ε) promoter. </LI> <LI> RA has an IgE-repressive activity in vivo, which may ameliorate IgE-mediated allergic disease. </LI> </UL> </P>

청각장애자 운동지도를 위한 프로그램 고찰

김정수,박정래,노일환,원충희,김흥식,강신일,박찬홍,김달영,이철원,이찬규 公州大學校스포츠科學硏究所 1987 스포츠科學硏究所論文集 Vol.- No.1

Hearing impairement in varying degrees constitutes one of the most common disabilites affecting school aged children. Eight out of every 1,000 students under the age of twenty have significant hearing loss. Hearing disabilities range from slight to total loss of sound perception. Statistics indicate that only one out of every ten hearing impaired children will become deaf. Most motor activities can be included students may possess low levels of static and/ or dynamic balance, but the greatest majority possess normal motor and physical capabilities. All the hearing impaired youngsters should be properly assessed and given the identical testing batteries as normal children. Hearing impaired students have, essentialy, a communication handicap and suffer from inability to understand verbal instructions. All physical educators should be able to use minimal finger spelling and signing for hearing impaired students.

석탄 비산재로부터 제올라이트의 합성 및 중금속 흡착능 평가

이창훈,유지호,신형상,정일래 서울産業大學校 2005 논문집 Vol.54 No.3

본 연구에서는 국내 영동화력발전소에서 발생하는 석탄 비산재(coal fly ash)로부터 KOH 용액을 사용한 수열반응을 통하여 제올라이트를 합성하고 중금속 흡착능을 평가하였다. 수열 합성 실험은 일정온도 조건 (100 ℃)에서 알칼리농도([KOH]:1 ~ 4 N), 고액비(S/L: 1/2 ~ 1/20 g/cm³) 및 반응시간 (1 ~ 96 hrs) 등의 합성조건을 달리하여 수행하였다. 그 결과 [KOH] = 3 ~ 4 N, 고액비(S:L) = 1:5 ~ 1:10, 반응시간 48 ~ 96 hr에서 200 ~ 240meq/100g의 CEC 값을 가진 제올라이트 생성물을 얻었다. XRD 분석결과 주요 결정상은 K-Chabazite이었으며 약 40%의 결정도를 보였다. 혼합 중금속(Cu, Cd, Cr, Pb)용액 (개별 중금속 농도 100mg/L, pH 5.0)을 대상으로 한 제올라이트 생성물의 중금속 흡착능르 평가한 결과, 제올라이트 첨가량 3g/200ml 이상에서 모든 중금속에 대하여 95% 이상의 제거율을 보였으며, 타 흡착제(천연제올라이트, 상업용 이온교환수지 등)와의 비교에서도 우수한 흡착능을 보였다.

진행성 비호지킨 림프종 환자에서 ICE 구제 요법에 의한 조혈모세포 가동화 후 자가 말초혈액 조혈모세포이식

이제중,이병환,김여경,변정래,이일권,박무림,정익주,김형준 대한조혈모세포이식학회 2003 대한조혈모세포이식학회지 Vol.8 No.1

연구배경: 비호지킨 림프종에서 자가 말초혈액 조혈모세포이식술이 폭넓게 이용됨으로 인해서, 높은 치료효과뿐만 아니라 조혈모세포의 가동화 효율이 높은 구제 요법이 요구되었다. 방법: 본 연구에서는 진행성 비호지킨 림프종에서 ICE 요법을 이용하여 조혈모세포를 가동화시킨 후 자가 말초혈액 조혈모세포이식이 시행하였던 환자를 후향적으로 분석하였다. 결과: 대상 환자의 중앙 연령은 38세(범위, 16~61)였으며, ICE 요법은 환자당 4주기(범위, 1~6주기)가 투여되었고, 투여 간격은 중앙값이 24일(범위, 16~36일)이었다. 고위험도 관해군을 제외한 13예의 환자 중 완전반응은 7예(53.8%), 부분반응은 4예(30.8%), 진행성질환은 2예(15.4%)를 보였다. 치료에 따른 3~4등급의 혈액학적 독성은 중성구감소증이 13예(81.3%), 혈소판감소증이 7예(42.8%)에서 관찰되었다. 조혈모세포 채집은 ICE 요법 후 중앙값이 12일(범위, 6~20일)에 시행되었고, 각 ICE 요법당 채집한 단핵구치는 중앙값이 5.75× 10^(8)/체중, CD34^(+) 세포는 중앙값이 1.25× 10^(6)/체중, CFU-GM치는 중앙값이 1.19× 10^(5)/체중이었다. 조혈모세포이식은 11예에서 시행되었고, 중앙 추적기간 401일에 평가한 2년 전체생존율은 70.1±14.7%, 1년 및 2년 무사건생존율은 각각 60.7±15.4%와 32.3±17.1%를 보였다. 결론: ICE 요법은 재발성/불응성 비호지킨 림프종 환자에서 높은 치료 반응률과 만족할 만한 조혈모세포 가동화를 보여 주었지만, 치료에 대한 순응도가 낮아서 투여 간격이 연장되는 문제점을 안고 있어서, 우리나라 환자의 실정에 적합한 구제요법에 대한 연구가 필요할 것으로 생각된다. Background: Due to the extensive application of high-dose chemotherapy with autologous peripheral blood stem cell transplantation (PBSCT), a salvage chemotherapeutic regimen with high response rate as well as effective capacity of PBSC mobilization is needed in non-Hodgkin's lymphoma (NHL). Methods: We analyzed the applicability of ICE (ifosphamide, carboplatin, and etoposide) regimen in NHL patients who underewent autologous PBSCT. Results: The median age was 38 years (range, 16~61 years), the patients received median 4 cycles (range, 1~6 cycles) of ICE regimen, and the median interval between each chemotherapeutic cycle was 24 days (range, 16~36 days). There were 7 (53.8%) complete responses, 4 (30.8%) partial responses, and 2 (15.4%) progressive diseases after ICE regimen. Toxicity included grade 3/4 neutropenia and throm bocytopenia in 13 (81.3%) and 7 (42.8%) patients, respectively. PBSC collection began on median day 12 (range, 6~20 days) after ICE therapy. The median number of mononuclear cells, CD34+ cells, and CFU-GM was 5.75× 10^(8)/kg, 1.25× 10^(6)/kg, and 1.19× 10^(5)/kg, respectively. With a median follow- up of 401 days, the patients who underwent autologous PBSCT had overall survival with 70.1±14.7% at 2 year and event free survival with 60.7±15.4% and 32.3±17.1% at 1 year and 2 years, respectively. Conclusion: ICE chemotherapy is an effective cytoreduction and mobilization regimen in patients with NHL, but profound myelosuppression with delayed recovery might pose difficulties in applying for Korean patients. Further evaluation for appropriate salvage regimens in Korean patients should be needed.

필라델피아 염색체 양성 급성골수성백혈병에서 Imatinib Mesylate로 분자학적 관해를 유도한 후 성공적인 자가 말초혈액 조혈모세포이식이 시행되었던 1예

김여경,이제중,이병환,변정래,조덕,이일권,정익주,양동욱,김형준 대한조혈모세포이식학회 2003 대한조혈모세포이식학회지 Vol.8 No.1

저자들은 필라델피아 염색체 양성 급성골수성백혈병 환자에서 복합항암화학요법으로 혈액학적 완전관해를 유도하고, imatinib mesylate를 이용하여 분자학적 관해를 유도한 후, 성공적으로 자가 말초혈액조혈모세포이식을 시행하였던 1예를 경험하였기에 이를 보고하는 바이다. Philadelphia chromosome (Ph¹)-positive acute myeloid leukemia (AML) is very rare disease, comprising 3~4% of the AML, and is a clinically distinct entity with poor clinical outcome with conventional chemotherapy. We report a case of Ph¹-positive AML who underwent successful autologous peripheral blood stem cell transplantation after achieving a molecular remission with imatinib mesylate.

후복막강내 발생한 Castleman병 1례

남성진,이동욱,박찬규,정시욱,김추성,조성래,구본천,양사규,이일웅,박정자 慶北大學校 醫科大學 1997 慶北醫大誌 Vol.38 No.2

Castleman' s disease is a rare, reactive lymphoid proliferation of unknown cause and pathogenesis. Depending on histololgic findings, it has been divided into two distinct histologic subtypes that include hyaline-vascular type(90%) and plasmacell type(10%). This disease occurs most commonly in mediastinal lymph nodes and cervical lymph nodes, inguinal lymph nodes respectively. But occurs rarely in retroperitoneum. We report a case of hyaline-vascular type asymptomatic Castleman' s disease of the retroperitoneum in a 47-year-old man.

일계성 딸기의 고온기 저온단일 처리가 화아형성 및 세대 촉진에 미치는 영향

노일래(Il Rae Rho),조용섭(Yong Seop Cho),정재완(Jae Wan Cheong),정호정(Ho Jeong Jeong),정해붕(Hae Boong Jeong) 한국원예학회 2007 원예과학기술지 Vol.25 No.1

딸기의 화아 유도는 온도와 일장 그리고 재배법 및 식물체의 영양상태에 민감하다. 본 실험은 ‘조홍’ב사치노카’, ‘조홍’, ‘사치노카’, ‘Elan’ 품종의 자식세대의 세대 촉진을 위해 인위적인 저온 단일처리를 함으로서 화아 유도에 필요한 생리적 조건을 검정하고자 수행하였다. 딸기 품종별 자식세대의 화아유도 및 세대촉진을 위한 저온(15℃)과 단일(일장 8시간)처리를 9주간 실시 한 결과 품종별 자식세대의 화아형성률은 파종 후 200일까지 ‘조홍’ב사치노카' 조합은 58.7%, ‘조홍’은 22.6%, ‘사치노카’는 54.4%, 대조품종인 ‘Elan’은 100%이었다. 따라서 딸기의 화아분화를 위해서는 저온단일이 필요하나 화아의 발육을 위해서는 고온장일 조건이 필요하였고, 딸기 실생묘의 저온단일 처리 감응에 필요한 묘령은 관부직경이 9~10㎜로 파종 후 일수로 계산하면 약 80일 이상이었다. Floral induction in strawberry is generally influenced by thermo-photoperiod and several agronomic and nutritional factors. This study was conducted to find out the environmental and plant physiological conditions for inducing the flower bud formation during the hot and long-day season. The progeny from a varietal cross of ‘Johong’ × ‘Sachinoka’ and three selfed generations from ‘Johong’, ‘Sachinoka’ and ‘Elan’ varieties were treated for 9 weeks with low temperature of 5℃ and short day-length of 8 hours during the su㎜er. The flower bud formation rate of the progeny selfed from an ever-bearing variety, ‘Elan’ was 100%, whereas that of the progeny crossed from ‘Johong’ × ‘Sachinoka’ and that of the progeny selfed from ‘Johong’ and ‘Sachinoka’ showed 58.7%, 22.6%, and 54.4%, respectively. As a result, it proved that low temperature and short photoperiod is essential for flower induction but high temperature and long day condition is also necessary for flower development in strawberry. Crown diameter of seedling to react on low temperature and short day treatments must to be at least 9-10 ㎜, and it need about 80 days from sowing.

Complete mitochondrial genome sequence of the red-spotted apollo butterfly, Parnassius bremeri (Lepidoptera: Papilionidae)

Man Il Kim,Iksoo Kim,Heon Cheon Jeong,Byung Rae Jin 한국응용곤충학회 2008 한국응용곤충학회 학술대회논문집 Vol.2008 No.10

The 15,389-bp long complete mitochondrial genome (mitogenome) of the redspotted apollo butterfly, Parnassius bremeri (Lepidoptera: Papilionidae) was determined. This genome has a gene arrangement identical to those of all other sequenced lepidopteran insects, but differs from the most common type, as the result of the movement of tRNAMet to a position 5’-upstream of tRNAIle. No typical start codon of the P. bremeri COI gene is available. Instead, a tetranucleotide, TTAG, which is found at the beginning context of all sequenced lepidopteran insects was tentatively designated as the start codon for P. bremeri COI gene. All protein-coding genes (PCGs), but COII (T) have complete termination codon TAA or TAG. One of the most unusual feature of the P. bremeri mitochondrial genome is the presence of two tRNA-like structures, such as the tRNATrp-like sequence and tRNALeu (UUR)-like sequence with proper anticodon and clover-leaf structures in the 514-bp long A+T-rich region. Furthermore, the A+T-rich region possesses three sequences that have the potential to form stem-and-loop structures, flanked by the conserved sequences, "TA(A)TA" at the 5’ end and "G(A)nT’ at the 3’ end. After more genomic and phylogenetic analyses are performed, further detailed information will be available.