비열 플라즈마 처리를 이용한 polystyrene, 소시지 케이싱, 그리고 훈제연어에서의 식중독균 저해

이한빛(Hahn-Bit Lee),노영은(Young-Eun Noh),양희재(Hee-Jae Yang),민세철(Sea Cheol Min) 한국식품과학회 2011 한국식품과학회지 Vol.43 No.4

PS 필름에 접종된 S. Typhimurium, E. coli 0157:H7, L. monocytogenes를 저해시킬 때 헬륨을 사용한 플라즈마 처리의 경우에는 S. Typhimurium이,헬륨과 산소를 동시에 사용한 플라즈마 처리의 경우에는 L. monocytogenes가 가장 많이 저해되었다. E coli 0157:H7과 L. monocytogenes는 헬륨과 산소를 함께 사용하여 풀라즈마 처리하였을 때 헬륨만 사용하였을 때보다 더 큰 저해를 보였다. 헬륨과 산소를 함께 사용한 비열 폴라즈마 처리는 L. monocytogenes가 소시지 케이싱이나 훈제연어에 감염되었 을 때보다 PS 필름에 감염되었을 때 L. monocytogenes를 더 효과적으로 저해시켜 감염된 재료에 따라 다른 저해 효과를 보여 주었다. 비열 폴라즈마 처리에 사용된 가스의 종류와 미생물이 감염된 재료는 그 처리의 항균 효과에 대한 평가에서 중요하게 고려되어야 할 것이다. 헬륨과 산소를 사용한 비열 플라즈마 처리는 공정 후 포장재에 감염된 L. monocytogenes를 효과적으로 저해할 수 있을 것으로 기대된다. The effects of nonthermal plasma treatments against Salmonella Typhimurium, Escherichia coli 0157:H7, and Listeria monocytogenes living on polystyrene (PS), sausage casings, and smoked salmon were investigated. Inoculated PS, casings, and salmon were treated with nonthermal plasma generated with helium (5 L/min) or with both helium (5 L/min) and oxygen (100 mL/min) at 60 Hz and 30 kVxm for 2, 5, or 10 min. S. Typhimurium exhibited the highest sensitivity to the helium-used treatment. The greatest reduction (3.9±0.8 log CFU/㎠) was observed with L. monocytogenes on PS after the treatment with the mixed gas for 5 min. The treatment with the mixed gas inhibited L. monocytogenes on casings and salmon by 0.5±0.3 log CFU/cm: and 1.0±0.3 log CFU g, respectively. Different treatment times did not result in different reductions of L. monocytogenes on both casings and salmon. The types of treatment gas and material of contamination need to be considered for evaluating the antimicrobial effects of nonthermal plasma treatments.

cDNA array 방법을 이용한 망간에 노출된 흰쥐 뇌기저핵의 유전자발현 분석

이채관,노성민,문덕환,,김정호,손병철,김대환,이창희,김휘동,김정원,김종은,안진홍,이채언 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Objectives: This study investigated the gene expression profile in basal ganglia of manganese-exposed rats based on cDNA array analysis. Methods: For cDNA array, 25 male Sprague-Dawley rats (250±25 g) were intraperitoneally injected with 25 ㎎/㎏ B. W./day of MnCl2 (0.3 ㎖) for 10 days. For dose-related gene expression analysis, rats were intraperitoneally injected with 0.2, 1.0, and 5.0 ㎎/㎏ B. W/day of MnCl2 for 10 days. Control rats were injected with an equal volume of saline. RNA samples were extracted from brain tissue and reverse-transcribed in the presence of [α^(32)P]-dATP. Membrane sets of the Atlas Rat 1.2 array Ⅱ and Toxicology array 1.2 kit (Clontech, Palo Alto, CA) were hybridized with cDNA probe sets. Northern blot hybridization method was employed to assess the dose-related gene expression. Results: Fifty-two genes showed significant changes in expression of more than two-fold. Twenty-eight were up-regulated and 24 were down-regulated in the manganese-exposed group compared to the control. Among the 52 genes, 28 genes including nuclear factor I-X1 (NF1-X1), neuroligin 2 and 3, mitochondrial stress-70 protein (MTHSP70), neurodegeneration-associated protein 1 (Neurodap 1), multidrug resistance protein (MDR), and endoplasmic reticulum stress protein 72 (ERP72), were reported for the first time related to the manganese-induced neurotoxic-metabolism in the rat basal ganglia. According to the dose-related gene expression analyses, MTHSP70, Neurodap 1 and ERP72 genes were up-regulated compared to the control even in the group exposed to low manganese dose ( 0.2 ㎎/㎏ B.W./day). Conclusions: Twenty-eight genes detected for the first time in this study were closely related to the manganese-induced neurotoxic-metabolism in the rat basal ganglia and further study of these genes can give some more useful information about the manganese metabolism.

대두와 Magnesium 급여가 당뇨쥐의 혈청과 간의 단백질 농도 및 효소활성에 미치는 영향

고진복,김재영,노민희 신라대학교 자연과학연구소 1996 自然科學論文集 Vol.2 No.-

Streptozotocin으로 유발시킨 당뇨쥐에 20% 생콩 및 20% 생콩에 마그네슘을 첨가한 식이로 4주간 사육하였다. 체중, 간, 신장 및 비장의 무게, 간과 혈청중 단백질농도, 그리고 혈청중 GOT, GPT 및 alkaline phosphatase의 활성에 미치는 영향을 조사하였다. 당뇨를 유발한 실험군은 당뇨병으로 인한 대사장애로 대조군에 비해 체중이 감소되었다. 간, 신장 및 비장의 무게는 대조군과 모든 당뇨군이 비슷하였으나, 체중에 대한 간, 비장 및 신장무게의 % 비율은 모든 당뇨군이 대조군보다 유의(p<0.05)하게 증가하였다. 간의 총 단백질과 albumin 농도는 대조군에 비해 모든 실험군에서 유의(p<0.05)하게 감소하였으나, 혈청의 총 단백질과 albumin의 농도 및 A/G ratio는 대조군과 각 당뇨실험군이 비슷한 수준을 보였다. Hemoglobin 농도와 hematocrit 치는 정상수준이었다. 혈청내 GPT 및 alkaline prosphatase의 활성은 대조군에 비해 모든 당뇨유발군이 유의(p<0.05)하게 증가되었다. 그리고 alkaline phosphatase의 활성은 당뇨대조군에 비해 당뇨생콩군과 당뇨생콩+Mg군은 다소 감소되었다. The effects of raw soy flour(RSF) and RSF with magnesium supplementation(RSF+ Mg) feeding on protein concentrations of liver and serum, and GOT, GPT and alkaline phosphatase activities of serum in diabetic versus nondiabetic rats were studied. Male rats(Spraque-Dawley ; mean weight 332.8±19.3g) were assigned to four dietary groups and fed experimental dietary for 28 days. For each experiment, some rats were injected with streptozotocin in intraperitonium(i.p.) to induce diabets, and the other rats were injected with buffer in i.p. as a nondiabetic group. The body weights of diabetic control, RSF and RSF+Mg groups were significantly decreased(p<0.05), but the total weights of liver, kidney and spleen in all the groups did not change compared with those of the nondiabetic group. Total protein and albumin concentrations of liver in all the groups were significantly lower(p<0.05) compared with those of the nondiabetic group. GPT and alkaline phosphatase activities in serum of all the groups were much more increased(p<0.05) than those of the nondiabetic group, but those of RSF and RSF+ Mg groups were similar to those of the diabetic control group.

분무조립에 의한 이트리아 안정화 과립분말의 제조

이종국,김민정,노희진,배지수 조선대학교 생산기술연구소 2000 生産技術硏究 Vol.22 No.1

Yttria-stabilized zirconia granule powder was prepared by a spray drying method, and the compacted and sintered behaviors were investigated Optimum slurry was prepared by the mixing of 70 vol% zirconia powder and 30% water and milling for 15 h in zirconia jar The slurry was stabilized by the addition of binder(PEG 155%, PVA 05%), dispersant(Cerasperse) 06%, Antifoaming agent(SN Defoamer) 03%, and Lubricant(Lu-6418) 1%, and the viscosity of this slurry showed the 5 dPas The optimum condition for the spray-drying of yttria stabilized zirconia powder is the inlet temperature of 110℃, outlet temperature of 90℃, slurry feeding rate of 140cc/min , and atomizer speed of 9000 rpm.

쌍태아에서 제 1태아의 분만 후 제 2태아의 지연분만 : 중례보고

김주환,서민정,유희정,노권일,조대현,박정규,이정헌,조성남,손영수 朝鮮大學校 附設 醫學硏究所 2005 The Medical Journal of Chosun University Vol.30 No.3

With the widespread use of fertility medications and techniques of in vitro fertilization, multiple gestation has become epidemic in modern obstetric practice. Delivery of the initial fetus in a multiple gestation usually is followed by delivery of the subsequent fetus or fetuses shortly thereafter. However in rare circumstances, the delivery is delayed for days due to disappearance of uterine contraction after delivery of the first fetus. When uterine activity ceases after the delivery of the first neonate, a policy of nonintervention may be considered in case of an immature or very premature delivery, in order to achieve a gestational age for the remaining fetus(es) more compatible with neonatal survival. We report a case of twin gestation with prolongation of the delivery interval between the twins for 46 days.

임부의 연령에 따른 보완대체요법에 관한 인식 태도 경험 비교 연구

김연진,김지연,노경민,신혜리,유예지,이지민,장희조,정아인,최소정,강숙정,이향연,민혜영 이화여자대학교 간호과학대학 2016 이화간호학회지 Vol.- No.50

Purpose: This study aims to test if the age of pregnant women affects the perception, experience, and attitude towards complementary alternative therapy (CAT), by investigating the perception, experience, and attitude of pregnant women under the age of 35, and above 35 (Advanced Maternal Age). The study will 1) find the general characteristics of pregnant women under and over the age of 35 2) compare the perception, behavior, and experience of pregnant women under and over the age of 35, and 3) find the comparison of the perception, behavior, and experience according to the general characteristics of pregnant women under and over the age of 35. Method: The subjects of this study are composed of 44 women under the age of 35 and 44 women above the age of 35, totaling 88 pregnant women visiting midwiferies, obstetrics and gynecology, and baby fairs in Seoul and Gyeong-Gi area. Data for this study was collected from November 28, 2015 to January 6, 2016 for 40 days. The collected data analyzed mean, standard deviation, t test, ANOVA, and chi-square by using SPSS 23.0. Result Results show that there is a significant difference in the perception, attitude, and experience of the two-sample groups in the use of CAT. The question of “what is most necessary when applying CAT in a hospital clinical environment” for the perception criteria came out with the results of t=9.980, p<.05. For the attitude, results showed that the answers of pregnant women under the age of 35 displayed a more positive attitude towards CAT. (t=2.421, p<.05) Lastly, in the experience section, pregnant women under the age of 35 showed more CAT use than the other sample group. Conclusion Results have shown that there is a difference in the perception, behavior, and experience between the two sample groups. Therefore further research on the efficacy and the adverse effects of CAT needs to be done.

사람 뇌척수액중 Transthyretin의 항산화작용

양성렬,최기오,박종근,류문희,홍석노,김수한,안봉환,이제혁,이민화 대한신경외과학회 1994 Journal of Korean neurosurgical society Vol.23 No.4

Protective effects of human cerebrospinal fluid antioxidants against enzyme inactivation caused by metal-catalyzed oxidation systems were investigated. When purified glutamine synthetase(GS) was incubated with human cerebrospinal fluid(CSF), the enzyme was progressively inactivated. Catalase and EDTA could inhibit the enzyme inactivation by 50-80%. Small-molecular(Mr< -10,000) fraction of CSF inactivated the exogenous GS, but large-molecular(Mr> -10,000) fraction did not. The GS inactivation by the small-molecular fraction was also markedly inhibited by catalase and EDTA. These results suggested that metal-catalyzed oxidation is involved in the GS inactivation by the small-molecular fraction of CSF. Dithiothreitol(DTT) was shown to inhibit almost completely the oxidative inactivation of GS by CSF. However, DTT inhibited only partially the oxidative inactivation of GS caused by small-molecular fraction of CSF. When large-molecular fraction of CSF was separated by anion-exchange HPLC chromatogrpahy, there was a peak of antioxidant activity inhibiting the small-molecular fraction-induced GS inactivation in the presence of DTT. The antioxidant activity was neutralized by monoclonal antibodies to transthyretin. Purified transthyretin was found to efficiently inhibit ascorbate/Cu^(2+)-induced GS inactivation in the presence of DTT. Uric acid and glucose did not show any protective effect on the GS inactivation in the same condition. The above results suggest that metal-catalyzed oxidation occurs normally in human CSF, and that transthyretin may play an important role as a CSF antioxidant in protecting proteins from metal-catalyzed oxidation.

간호대학생의 메타인지, 학습동기, 학업적 자기효능감이 자기주도학습능력에 미치는 영향

김소희(So Hee Kim), 이정민(Jung Min Lee), 김민경(Min Gyeong Kim), 김수아(Su Ah Kim), 김주은(Ju Eun Kim), 노윤서(Yun Seo Noh), 송지민(Ji Min Song), 이다원(Da Won Lee), 이소희(So Hee Lee) 다문화건강학회 2023 다문화건강학회지 Vol.13 No.1

Purpose: This study sought to identify the influence of metacognition, learning motivation, and academic self-efficacy on the self-directed learning ability of nursing students. Methods: A descriptive study design was used, and data were collected using self-reported questionnaires from 194 nursing students. Statistical analyses, including independent t-test, one-way ANOVA, Pearson's correlation, and multiple regression analysis, were performed using SPSS 26.0. Results: Metacognition, learning motivation, and academic self-efficacy significantly predicted nursing students' self-directed learning ability, accounting for 66.3% of the variance. Conclusion: Interventions to improve the self-directed learning ability of nursing students should focus on enhancing metacognition, learning motivation, and academic self-efficacy.

Contribution Analysis of Interior Vibration in High-speed Train by OTPA

Hee-Min Noh(노희민),Sang-Soo Kim(김상수),Suk-Yoon Hong(홍석윤),Jee-Hun Song(송지훈),Hyung-Woung Kwon(권현웅),Jea-Deok Jung(정재덕) 한국소음진동공학회 2016 한국소음진동공학회 학술대회논문집 Vol.2016 No.10

Investigation of noise sources in high-speed trains

Noh, Hee-Min,Choi, Sunghoon,Hong, Sukyoon,Kim, Seog-Won Professional Engineering Publishing Ltd 2014 Proceedings of the Institution of Mechanical Engin Vol.228 No.3

<P>In this paper, the quantification of the external noise sources in high-speed trains is discussed. A thorough understanding of the underlying causes of noise generation in high-speed trains is needed to develop effective noise control measures. However, because high-speed trains produce a complex array of sounds, it is very difficult to determine each individual source of noise. In this study, the delay-and-sum beamforming method, which uses microphone arrays, was used to separate the noise sources and analyze the sound characteristics of high-speed trains. A new microphone array with 96 microphones was designed to measure the noise produced by high-speed trains. Performance verification tests were conducted to ensure the reliability of the results obtained from the array. Then, the system was used to measure the sounds produced by Korean high-speed trains traveling at speeds between 150 and 300 km/h. Sound maps were then produced using the beamforming technique. The study determined that the majority of the noise produced by the high-speed trains originated from the front nose, bogie, pantograph and inter-coach spacing. Finally, the beampower spectra of the aerodynamic noise sources originating in the front nose, pantograph and inter-coach spacing were deduced from frequency conversion. From these results, the aerodynamic noise characteristics of the major sources of noise in high-speed trains were determined.</P>