망간 노출이 흰쥐의 정소기능과 혈청프로락틴 농도에 미치는 영향

이채관 한국발생생물학회 2009 발생과 생식 Vol.13 No.4

망간은 정소독성을 나타내며 뇌기저핵에 작용하여 혈청프로락틴의 농도를 증가시킨다. 그리고 혈청 프로락틴 농도 상승에 의한 과프로락틴혈증(hyperprolactinemia)은 정소의 정자생성을 억제한다. 본 연구에서는 망간의 전신노출이 흰쥐 정소의 정자생산과 혈청 프로락틴 농도에 미치는 영향을 조사하기 위하여 실험동물을 대조군 (0.0 ㎎/㎥)과 망간 노출군(Mn 1.5 ㎎/㎥)으로 나누고 노출군은 다시 노출 기간에 따라 4주와 13주 노출군 등 4군으로 분류하였다(n=10). 노출 기간에 따라 실험동물의 체중 변화와 사료 섭취량 등 일반적 소견 관찰, 혈액과 정소의 망간 농도, 정자의 수와 기형 등을 관찰하였다. 그리고 망간 노출에 따른 혈청 프로락틴 농도를 조사하여 망간 노출 조건에 따른 혈청프로락틴 농도 변화 및 정소독성을 조사하였다. 망간 노출 4주 및 13주군에서 노출기간에 따라 혈액 및 정소의 망간 농도가 유의하게 증가되었다. 대조군에 비하여 망간 노출군에서 노출기간에 따라 정자의 수가 감소되었으며, small head와 bent tail 등 기형 정자의 빈도는 증가하였다. 혈청 프로락틴의 농도는 망간 투여군에서 대조군에 비하여 유의하게 증가하였다. 그러나 실험동물의 체중 변화 및 사료 섭취량은 실험군간에 차이가 없었다. 이러한 결과들로 보아 1.5 ㎎/㎥ 농도의 아만성 망간 노출은 흰쥐의 혈청 프로락틴 농도를 증가시키고, 정소독성의 원인으로 추정된다. 그리고 전신노출에 의한 망간의 흰쥐 정소독성의 무유해영향농도(NOAEL)는 1.5 ㎎/㎥ 이하로 예측된다.

발생중인 계배조직에서의 POU - domain 유전자 발현에 관한 연구

이채관,강성구 한국유전학회 1994 Genes & Genomics Vol.16 No.3

The expression of POU -domain gene was detected in the central nervous system and the stages of nerve-related-organogenesis during early embryogenesis. The POU-domain gene products, or transcription factors, may exert critical function in the differentiation of special cell types as well as in the activation of specific programs of gene expression that determind specific cell phenotypes within a certain organ. The POU-domain gene expression during chick embryogenesis was analysed by hybridizing 32P-dCTP labelled chicken POU domain which was homologue of the conserved mouse POU-homeo gene, Brn with the total RNAs of 2 days, 7 days and 9 days chick whole embryos. Also the mRNAs of brain, liver, kidney and breast muscle of 12 days and 14 days chick embryos were analysed by using northern hybridization. Based on the results of dot and northern hybridization of chick embryonic RNAs and POU domain probe, it appears that POU -domain gene expression began from 2 days chick embryos and is gradually increased on to 9 days. Also the chick POU-domain gene is expressed in brain, kidney and breast muscle of 12 days and 14 days embryo. The expression of chick POU-homeo gene in kidney is much increased in 14 days embryo than in 12 days, but not detected in 12 days nor 14 days chick embryo in liver. As a conclusion, the expression of chick POU-domain gene appears to begin in early embryogenesis and to be reinforced later in the ectoderm related embryonic organs.

태아독성 관련 실험적 연구사례

이채관,이종태,김대환,손병철,안진홍 대한직업환경의학회 2009 대한직업환경의학회 학술대회 논문집 Vol.43 No.-

자원순환시설 주민건강영향조사 개선방향

이채관,서춘희,박동윤,최영태,양원호 한국환경보건학회 2022 한국환경보건학회 학술대회 자료집 Vol.2022 No.5

지자체 소각로 주변 건강영향평가 방법

이채관,서춘희,박동윤,최영태,양원호 한국환경보건학회 2021 한국환경보건학회 학술대회 자료집 Vol.2021 No.2

cDNA array 방법을 이용한 망간에 노출된 흰쥐 뇌기저핵의 유전자발현 분석

이채관,노성민,문덕환,,김정호,손병철,김대환,이창희,김휘동,김정원,김종은,안진홍,이채언 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Objectives: This study investigated the gene expression profile in basal ganglia of manganese-exposed rats based on cDNA array analysis. Methods: For cDNA array, 25 male Sprague-Dawley rats (250±25 g) were intraperitoneally injected with 25 ㎎/㎏ B. W./day of MnCl2 (0.3 ㎖) for 10 days. For dose-related gene expression analysis, rats were intraperitoneally injected with 0.2, 1.0, and 5.0 ㎎/㎏ B. W/day of MnCl2 for 10 days. Control rats were injected with an equal volume of saline. RNA samples were extracted from brain tissue and reverse-transcribed in the presence of [α^(32)P]-dATP. Membrane sets of the Atlas Rat 1.2 array Ⅱ and Toxicology array 1.2 kit (Clontech, Palo Alto, CA) were hybridized with cDNA probe sets. Northern blot hybridization method was employed to assess the dose-related gene expression. Results: Fifty-two genes showed significant changes in expression of more than two-fold. Twenty-eight were up-regulated and 24 were down-regulated in the manganese-exposed group compared to the control. Among the 52 genes, 28 genes including nuclear factor I-X1 (NF1-X1), neuroligin 2 and 3, mitochondrial stress-70 protein (MTHSP70), neurodegeneration-associated protein 1 (Neurodap 1), multidrug resistance protein (MDR), and endoplasmic reticulum stress protein 72 (ERP72), were reported for the first time related to the manganese-induced neurotoxic-metabolism in the rat basal ganglia. According to the dose-related gene expression analyses, MTHSP70, Neurodap 1 and ERP72 genes were up-regulated compared to the control even in the group exposed to low manganese dose ( 0.2 ㎎/㎏ B.W./day). Conclusions: Twenty-eight genes detected for the first time in this study were closely related to the manganese-induced neurotoxic-metabolism in the rat basal ganglia and further study of these genes can give some more useful information about the manganese metabolism.

Bisphenol A가 흰쥐의 태반 기능과 출산에 미치는 영향

이채관,김석현,문덕환,김정호,손병철,김대환,이창희,김휘동,김정원,김종은,이채언,Lee, Chae-Kwan,Kim, Seog-Hyun,Moon, Deog-Hwan,Kim, Jeong-Ho,Son, Byung-Chul,Kim, Dae-Hwan,Lee, Chang-Hee,Kim, Hwi-Dong,Kim, Jung-Won,Kim, Jong-Eun,Lee, Chae 대한예방의학회 2005 예방의학회지 Vol.38 No.3

Objectives : The aim of this study was to investigate the effects of bisphenol A (BPA), an estrogen-like environmental endocrine disrupter, on the placental function and reproduction in rats. The mRNA levels of the placental prolactin-growth hormone(PRL-GH) gene family, placental trophoblast cell frequency and reproductive data were analyzed. Methods : The pregnancies of F344 Fisher rats ($160g{\pm}20g$) were detected by the presence of the copulatory plug or sperm in the vaginal smear, which marked Day 0 of pregnancy. Pregnant rats were divided into three groups. The control group was intraperitoneally injected with a sesame oil vehicle. The two remaining groups were injected with 50 or 500 mg/kg B.W/day of BPA, resuspended in sesame oil, on either days 7 to 11 or 16 to 20 of pregnancy, with the rats sacrificed on either day 11 or 20, respectively. The mRNA levels of PRL-GH and Pit-1a and b isotype genes were analyzed by Northern blot hybridization and reverse transcription-polymerase chain reaction. The hormone concentrations were analyzed by radioimmunoassay, and the frequency of the placental trophoblast cells observed by a histochemical study. Reproductive data, such as the placental weight and litter size, were surveyed on day 20. The fetal weight was surveyed for 4 weeks after birth. A statistical analysis was carried out using the SAS program (version 8.1). Results : The mRNA levels of the PRL-GH gene family, such as placental lactogen I, Iv and II, prolactin like protein A, C and Cv, and decidual prolactin-related protein were significantly reduced due to BPA exposure. The mRNA levels of the Pit-1a and b isotype genes, which induce the expression of the PRL-GH gene family in the rat placenta, were also reduced due to BPA exposure. The PL-Iv and PL-II concentrations were reduced in the BPA exposed group. During the middle to last stage of pregnancy (Days 11-20), a high dose of BPA exposure reduced the frequency of spongiotrophoblast cells, which are responsible for the secretion of the PRL-GH hormones. Reproductive data, such as the placental and fetal weights and the litter size, were reduced, but that of the pregnancy period was extended in the BPA exposed compared to the control group. Conclusions : BPA disrupts the placental functions in rats, which leads to reproductive disorders.

일반인 전혈의 과불화화합물(PFCs) 농도와 관련요인조사

이채관,이종태,김정호,김대환,손병철,이창희,김휘동,서춘희 대한직업환경의학회 2010 대한직업환경의학회 학술대회 논문집 Vol.45 No.-

만성요통을 가진 중년여성에게 동적 신경근 안정화 운동이 균형, 보행 및 요통장애지수에 미치는 효과

이채관 한국체육무용과학회 2023 Journal of Sport and Dance Science Vol.3 No.2

본 연구는 만성요통을 가진 중년여성을 대상으로 동적 신경근 안정화 운동이 균형, 보행, 요통장애지 수에 미치는 효과를 알아보기 위해 수행되었다. 이와 같은 연구목적 달성을 위해 만성요통이 있는 40명 의 중년여성을 연구 대상자로 선정하여 두 집단으로 나누어 중재 전 후를 비교분석하였다. 연구 결과도 출을 위하여 균형은 힘판위에서 한발서기 검사, 보행은 동작분석기, 요통장애지수는 Oswestry Disability Index를 사용하였다. 자료처리 방법은 IBM Statistics SPSS Win ver. 28.0 프로그램을 사용하였다. 집 단 내 중재 전과 후의 균형, 보행의 시공간적 분석, ODI의 변화를 비교하기 위해 대응표본 t-검정 (paired t-test)을 실시하였으며, 집단 간 중재 전과 후의 변화량 차이를 비교하기 위해 독립표본 t-검 정을 실시하였다. 이상과 같은 연구 방법을 적용하여 도출된 결론은 다음과 같다. 첫째, 동적 신경근 안정화 운동을 시행한 실험집단 중년여성들의 균형이 향상되었다. 둘째, 동적 신경근 안정화 운동을 시행한 실험집단 중년여성들의 보행의 시공간적 변수가 개선되었다. 셋째, 동적 신경근 안정화 운동을 시행한 실험집단 중년여성들의 요통장애지수가 개선되었다. Objectives The purpose of this study is to examine the effect of dynamic neuromuscular stabilization exercise on balance, gait, and oswestry disability index in middle-aged women with chronic low back pain. Methods The subjects of this study were middle-aged women with chronic low back pain, and the 20 subjects in experimental group the that performed dynamic neuromuscular stabilization exercise and the 20 in the control group were randomly assigned. Balance, gait, and Oswestry Disability Index were measured before and after the intervention. For the measured data, a paired t-test was performed to determine the amount of change before and after the intervention within each group, and an independent t-test was performed to compare the amount of change before and after the intervention between the groups. Results Left-right sway distance, anterior-posterior sway distance, total sway distance in single leg balance test for static balance, were significantly reduced only in the experimental group, improving static balance. Only in the experimental group, gait velocity, cadence, and step length, which are spatiotemporal variables of gait, increased significantly, and double support time, which is a spatiotemporal variables of gait, decreased, leading to improved gait ability. The oswestry disability index, an indicator of activities of daily living, was significantly decreased in both the dynamic experimental group and the control group, and daily living function improved. Conclusions Dynamic neuromuscular stabilization exercise is an effective training method as an intervention for the improvement of balance, balance, gait spatiotemporal variables, kinematic variables, and Oswestry Disability Index in middle-aged women with chronic low back pain.

메틸수은 노출이 흰쥐의 태반 효율과 태아 성장에 미치는 영향

이채관(Chae Kwan Lee) 한국환경보건학회 2020 한국환경보건학회지 Vol.46 No.4

Objectives: Some animal studies have reported that methyl mercury causes developmental toxicities such as placental and fetal weight loss, but the mechanism is still unclear. This study aimed to investigate the developmental toxicities of methyl mercury, focusing on placental endocrine function and fetal growth retardation in rats. Methods: Positively same-time-mated female Sprague-Dawley rats were purchased on gestational day (GD) eight and treated with 0, 5, 10 and 20 ppm of methyl mercury (n=5) dissolved in tap water from GD eight through 19. During treatment, the drinking water (methyl mercury) intake and body weight of each pregnant rat was measured daily. On day 19, caesarean sections were performed and blood samples were collected. Developmental data such as placental and fetal weights, fetus numbers, and placental efficiency (fetal weight/ placental weight) were also collected. Placental prolactin-growth hormone (PRL-GH) family, such as placental lactogen (PL) -Iv, II, and prolactin-like protein (PLP) -B, levels in serum were analyzed by ELISA. Also, placental tissues were assigned to histochemistry. Results: The mean cumulative methyl mercury exposure for the 5, 10, and 20 ppm groups were 2.37, 4.63, and 9.66 mg, respectively. The mean daily exposure of the 5, 10, and 20 ppm groups were 0.24, 0.47, and 0.97 mg, respectively. Maternal body weight increased in accordance with GD. There was no significant difference in weight gain among the experimental groups. Histopathologic changes were not observed in placental tissues among the experimental groups. However, mean placental and fetal weights were lower in the 10 and 20 ppm exposed groups compared to the control. Placental efficiency was also lower in the 10 and 20 ppm exposed groups compared to the control. Serum PL-Iv and II levels were lower in the 10 and 20 ppm exposed groups than the control, in accordance with the changing pattern of placental and fetal weights and placental efficiency. Conclusion: The inhibitory effects of methyl mercury on the serum levels of placental PRL-GH family such as PL-Iv and II may be secondary leads to the reduction of placental efficiency and fetal growth retardation in rats.