구형 Helicobacter pylori의 중성구 매개 산화활성

홍원선,김혜원,이학성,명승재,정훈용,양석균,김진호,민영일,정명희 대한암예방학회 2000 Journal of cancer prevention Vol.5 No.3

함암요법으로 골수기능이 억제된 악성종양환자에서 rhG-CSF의 임상적 효과

김원민,서영환,조경상,유병전,김상도,이승일,정춘해 朝鮮大學校 附設 醫學硏究所 1992 The Medical Journal of Chosun University Vol.17 No.2

We have studied the efficacy of rhG-CSF in patients with non-hodgkin's lymphoma, acute leukemia, and small cell lung cancer undergoing anticancer chemotherapy. These patients were below leukocyte count 3,000 cubic millimeter due to myelosuppression induced by the first cycle of intensive chemotherapy. Treatment with rhG-CSF (100㎍ per square meter of body surface area per day in a 30-minute intravenous infusion) was begun two days and for 14 consecutive days after the end of the second cycle of chemotherapy. The results were as follows. 1. The onset of myelosuppression was 6 days after chemotherapy, and the onset of recovery was 16.7 days after chemotherapy, and the duration of granulocytopenia was 10.7 days in patients with malignant tumor during contrast period. 2. The duration of granulocytopenia was shortened 5.2 days in patients administered rhG-CSF than without rhG-CSF, and we observed the shortest duration of granulocytopenia with increasing granulocyte in patients with small cell lung cancer. 3. Observing the differential count of leukocyte checked the highest level of leukocyte in contrast and rhG-CSF period, the persentage of neutrophil was increased in patients with acute leukemia and small cell lung cancer during rhG-CSF period, but not increased in patients with non-hodgkin's lymphoma. 4. rhG-CSF was not influenced on liver faction, renal fuction, uric acid, and glucose metabolism, also had no effect on recovery of platelet. In conclusion, rhG-CSF can be administered to patients with hematologic or nonhematologic malignant tumor that myelosuppression induced by anticancer chemotherapy result in shortening the duration of granulocytopenia and increasing the peripheral neutrophil, therefore full dose chemotherapy can be administered on time, and rhG-CSF may reduce the morbidity and mortality of patients with malignant tumor undergoing chemotherapy.

사람 위선암에서 cathepsin L의 발현증가

홍원선,홍석일,박인철,손영숙,정훈용,양석균,김해련,민영일 울산대학교 의과대학 1996 울산의대학술지 Vol.5 No.1

cathepsin L은 lysosomal cysteine 단백분해효소로서 기저막(basement membrane)과 세포외기질(extracellular matrix)을 파괴하여 암세포의 침윤과 전이에 중요한 역할을 하는 물질로 알려져 있다. 이러한 cathepsin L에 대한 mRNA 발현도를 5개의 사람 위선암(gastric adenocarcinoma) 세포주와 5명의 위선암 환자에서 채취한 조직에서 방사능으로 표지된 cathepsin L특이 cDNA를 사용한 Northern blot법으로 측정하였다. 위암의 전이병소에서 수립한 세포주인 SNU-5, SNU-16, MKN-45와 Kato Ⅲ에서는 cathepsin L mRNA가 발현되었으나 원발병소에서 수립한 AGS 세포주에서는 mRNA의 발현이 관찰되지 않았다. 5명의 위암 환자에서는 원발병소, 전이가 확인된 임파절 및 암 근처 정상 위점막에서 각각 조직을 채취하여 cathepsin L mRNA의 발현을 측정하였다. 원발병소와 전이병소에서는 모두 cathepsin L mRNA가 발현되었으나 정상 위점막조직에서는 전예에서 mRNA 발현이 관찰되지 않았다. 한편 mRNA의 발현도는 1예에서는 전이병소가 원발병소에 비해 높았으나, 2예에서는 전이병소에서 발현도가 낮았으며, 나머지 2예에서는 원발병소와 전이병소 사이에 차이가 없어, 원발병소와 전이병소 사이에 mRNA의 발현도의 일관성 있는 경향은 관찰되지 않았다. 이상의 결과는 cathepsin L은 위암의 발생과 진행에 있어 암세포의 침윤과 전이를 촉진하는 것 이외에 또 다른 역할을 할 가능성을 시사하고 있다고 사료된다. Cathepsin L, a lysosomal cysteine protease, is known to play an important role in cancer invasion and metastasis by degrading the components of basement membrane and extracellular matrix. The mRNA expression of cathepsin L was determined by Northern blot analysis using a radiolabeled cDNA specific for cathepsin L in five human gastric adenocarcinoma cell lines and five surgical specimens of primary gastric adenocarcinomas, their metastatic lymph nodes and matched adjacent normal mucosae. The mRNA of cathepsin L was expressed in all of the four cell lines established from the metastatic sites, SNU-5, SNU-16, MKN-45 and Kato Ⅲ, while not detected in one cell line established from the primary site, AGS. The mRNA was expressed in all of the five primary and five metastatic cancer specimens tested, while it was not detected in all matched normal mucosae. The intensities of the mRNA expressions, however, did not show the consistent pattern between primary sites and metastatic lymph nodes. These results suggest that cathepsin L may have the other function in addition to facilitation of the invasion and metastasis during the development and progression of stomach cancer.

한국산 뱀독이 개구리 심장심실 근육 세포의 활동전압에 미치는 영향

정해성,방효원,엄대용,이상돈 중앙대학교 의과대학 의과학연구소 1984 中央醫大誌 Vol.9 No.2

Action potential of a frog ventricular cell was studied by conventional 3M KCI glass microelectrodes to investigate the effect of freeze-dried Korean snake venom. Following results were obtained; 1. Time to repolarize 50% of its action potential increased from 756±26mS to 796±17mS. 2. The notch which would be seen after the fast depolarization disappeared and the overshoot potential significantly decreased from 24.4±0.9㎷ to 17.8±2.3㎷. 3. Positive depolarization rate (dV/dt max) decreased from 22.8±0.8V/s to 18.8±0.8V/s. 4. Resting membrane potential was -95±3.0㎷ and showed little change. Above results would be interpreted that the Korean snake venom blocked the fast sodium channel and slow calcium channel simultaneously to reduce the contractility and activation of potassium channel which could result the prolongation of action potential duration.

어린이 식생활 교육을 위한 만화와 애니메이션의 개발

김보경,박혜련,김정원 대한지역사회영양학회 2008 대한지역사회영양학회지 Vol.13 No.5

The purpose of this study was to develop children-friendly comic books and animation for dietary education of 3rd or 4th grade elementary students. Through a literature review on current dietary problems and dietary education for children, 15 educational themes were chosen on both healthy eating and safe dietary life. Comic books and animation were developed based on the themes by the brainstorming and help of professional animators, and they were applied and evaluated in the field. The developed comic books and animation, with its own characters, proved to be of high quality and effective educational materials for children’s healthy and safe eating. Compared with the reference group, the tested group has shown a significant improvement in dietary knowledge and attitude (p < 0.05). And both parents (level of understanding 4.29, level of interest 4.49, level of usefulness 4.46, level of design satisfaction 3.95 in 5-point Likert scale) and children (level of understanding 4.63, level of interest 4.57, level of usefulness of 1st comic book 4.44, 2nd comic book 4.49, level of design satisfaction 4.06, level of usefulness for knowledge pages 4.22 in 5-point Likert scale) have shown high levels of interests and satisfaction. Therefore, these materials could be utilized as effective educational materials for elementary students in school lunch time, extra-curricular activities, or after school programs

조직구성 수성망상증 2예

최연수,박일,죄희충,김원식,양태영,허경,안기완,정춘해 조선대학교 1993 The Medical Journal of Chosun University Vol.18 No.2

Histiocytic medullary reticulosis (HMR) is a malignant noeplastic process, with tumor cells belonging to the histiomonocytic line, and originally described by Scott and Robb-Smith in 1939 characterized by its acute onset, progression to death within a few months and its clinical features are fever, lymphadenopathy, hepatosplenomegaly, jaundice, and purpura common laboratory findings are characterized by severe anemia, leukopenia, thrombocytopenia and histologically by systemic progressive invasion of morphologically atypical histiocytes or of their precursor. 61 and 37-year-old males were admitted with camplaints of high fever and chilling sensation and were treated by cyclophosphamide, vincristine, prednison and antiobiotics, but patients all died 17 and 14 days after admission due to probably sepsis and respiratory failure.

용사코팅용 나노 WC-12Co 피드스톡의 제조

전형우,김주선,박준영,박종구,황순영,이해원 대한금속재료학회 2004 대한금속·재료학회지 Vol.42 No.4

This study investigates the fabrication of WC-12Co feedstock powders with weakly locculated slurries by spray drying under various conditions. The morphological defects and flocculation states according to the amount of additives are evaluated to prepare spherical WC-Co feedstock powders with little morphological defects and homogeneous microstructures. The characteristics of feedstock powders, heat treated between 850-1200℃, are analyzed via SEM, EPMA, and mercury porosimetry. The results showed that with the increase in heat treatment temperature the portion of larger pores around 10 μm increased while, that of smaller pores of 1 μm lessened due to the coalescence of WC particles, forming dense feedstock powders with little defects such as hollow or craters. The feedstock heat treated at 1200℃ showed a porosity of 42.2% and a density of 8.28 g/cm^(3) as well as uniform distribution of pores and Co. (Received January 19, 2004)

면접조사를 통한 초등학생의 영양교육 실태 및 실천도 조사

오유진,이영미,김정현,안홍석,김정원,박혜련,서정숙,김경원,권오란,박혜경,이은주,성현이 대한지역사회영양학회 2008 대한지역사회영양학회지 Vol.13 No.4

This study investigated the experience and practice of elementary school students on nutrition education. The data were collected from 217 male and female students attending 5-6th grade elementary schools in Seoul and Kyunggi-Do from March to June 2007, interviewing face to face by a nutrition teacher and 3 interns of a nutrition teacher. The results were as follows: 86.5% of the subjects learned about ‘Table etiquette’, ‘Reasons for eating fruits and vegetables’ (78.7%), ‘Food waste and environment’ (72.3%), ‘Healthy snacks’ (55.7%), ‘Food sanitation’ (52.3%), ‘Food culture of foreign countries’ (48.1%). Nutrition education experience was significantly different by gender. A total of 43.5% boys responded that they never learned about ‘basic food preperation’ (p < 0.01). They had learned ‘Nutrients for body’ and ‘Food waste and environment’ in school, ‘Healthy weight loss’, ‘Food culture of foreign countries’, ‘Food circulation’ on television, Most content (‘Table etiquette’, ‘Simple cooking’, ‘Food sanitati n’, ‘Eating behaviors for health’, ‘Reasons for eating fruits and vegetables’, ‘Healthy snacks’) was learned from parents. The practice after nutrition education was higher in ‘Table etiquette’ (2.14), ‘Eating fruits and vegetables’ (2.07) than others compared with education experience. The most reason of non-practice on nutrition information was ‘Troublesome’. In ‘Nutrients for body’, a boy answered ‘Difficult for practice’ 20.0%, a girl answered ‘Difficult to understand’ 32.6%, showing a significant difference between the gender groups (p < 0.001). They remembered the ‘Nutrients for body’ (49.6%), ‘Food sanitation’ (44.5%) because of ‘important content’,‘Basic food preparation’ (40.6%), ‘Food culture of foreign countries’ (36.3%) because of ‘interesting content’, ‘Healthy weight loss’ (52.0%), ‘Eating behavior for health’ (44.5%) and ‘Healthy snacks’ (33.7%) because of ‘need for my health’.

출혈성 소화성 궤양의 장기 재발률

심기남,정훈용,양석균,홍원선,박의련,박무인,김해련,민영일 대한소화기내시경학회 1999 Clinical Endoscopy Vol.19 No.2

Background/Aims: Bleeding from a peptic ulcer is one of the common and serious complications associated with the rate of reported mortality, which ranges from 5% to 10%. Endoscopic therapy is effective in controlling active bleeding and reducing the emer-gency surgery, the immediate mortality rate and the incidence of early rebleeding. But few recent studies have documented the long-term recurrent bleeding rate after discharge in patients with bleeding peptic ulcers. The aim of this study was to determine the long-term recurrent bleeding rate and factors predisposing to recurrent bleeding. Methods: Eighty-eight patients with bleeding peptic ulcers discharged after medical treatment between Dec. 1990 and Jul. 1992 were included in this study and retrospectively followed up with medical records and telephone interviews. The end point of follow-up was recur-rent hemorrhage, surgery for treatment of ulcer complication, or death. Results: By July 1997, retrospective follow-up was available in 76 patients. Recurrent bleeding occurred in 23 patients (30.3%) with bleeding peptic ulcers and the median follow-up period was 69 months (range, 1 ∼79 months). The estimated cumulative recurrent bleeding rate after 1, 2, 3, 4, 5 and 6 years was 11.8%, 14.5%, 19.9%, 24.2%, 27.2% and 34.2%, respectively. There was no difference between the recurrent bleeding group and the non-recurrent bleed-ing group according to age, sex, prior NSAIDs use, previous history of bleeding or pepticulcer, site of ulcer, stigmata of recent hemorrhage at initial examination, method of treatment and amount of transfusion. Conclusion: Recurrent bleeding occurred in one-third of patients with bleeding peptic ulcers after 6 years of follow-up and one-third of recurrent bleeders rebled within 1 year. The factors predisposing to recurrent bleeding in the long-term follow-up could not be found. Therefore, further studies designed to identify factors predisposing to recurrent bleeding are needed and the evaluation of Helicobacter pylori status in bleeding pepic ulcer is needed because Helicobacter pylori is an important factor of peptic ulcer recurrence.

세침 흡입 생검으로 진단된 간의 혈관근지방종 1 예

이경아,유은실,민영일,김해련,심기남,정훈용,양석균,홍원선,박의련,이문규 대한소화기학회 1999 대한소화기학회지 Vol.33 No.6

Hepatic angiomyolipoma is a rare benign tumor of the liver composed of blood vessel, smooth muscle cells, fat and myelocomponent. The preoperative diagnosed of the lesion is important because of its therapeutic implications. Radiologic findings are not specific because the composed elements are variable in proportion and distribution. Thus, the findings at computed tomography, ultrasono graphy and magnetic resonance imaging may be only suggestive and its definitive diagnosed requires histologic confirmation. We experienced a case of hepatic angiomyolipoma in patient with chronic hepatitis diagnosed preoperatively by fine-needle aspiraton biopsy under ultrasound guidance. To our knowledge, this is the first case of hepatic angiomyolipoma diagnosed preoperatively in Korea. We report it with review of literatures.