당뇨병 환자의 적혈구에서 항산화효소 활성도의 변화

유병전 ( Yu Byeong Jeon ),배학연 ( Bae Hag Yeon ),이병래 ( Lee Byeong Lae ) 대한내과학회 1993 대한내과학회지 Vol.44 No.6

저자는 1992년 3월부터 1992년 8월까지 조선대학교 부속병원 내과에 입원중인 당뇨병 환자 60명을 대상으로 적혈구에서 항산화효소 활성도를 측정하고 20명의 대조군과 비교하여 다음과 같은 결과를 얻었다. 1) 당뇨병 환자의 적혈구에서 SOD 활성도는 2.204±0.711U/mg Hb로 대조군의 3.070±0.721U/mg Hb에 비해 유의한 감소를, 그리고 G6PDH의 활성도는 0.648±0.241U/mg Hb로 대조군(0.456±0.190U/mg Hb)에 비해 유의한 증가를 보였다(p<0.05). 2) 당뇨병의 이환기간이 10년 이상인군의 적혈구 glutatione peroxidase 활성도는 0.74±0.17U/mg Hb로, 대조군(1.81±0.48U/mg Hb), 5년 이하군(1.26±0.38U/mg Hb), 및 5~10년군(1.45±0.42U/mg Hb)에 비해 유의한 감소를 보였다(p<0.01). 3) 당뇨병성 망막증이 있는 군의 적혈구 glutathione peroxidase 활성도는 0.74±0.27U/mg Hb으로 대조군(1.81±0.48U/mg Hb, p<0.01) 및 망막증이 없는군(1.47±0.54U/mg Hb, p<0.05)에 비해 유의한 감소를 보였고, 망막증이 있는 군의 적혈구 catalase 활성도는 2.93±0.51U/mg Hb로 망막증이 없는(4.01±0.28U/mg Hb)에 비해 유의한 감소를 보였다(p<0.05). 4) 당뇨병성 신증이 있는 군의 적혈구 총 glutathione량은 10.61±4.48nM/mg Hb로 당뇨병성 신증이 없는군(15.90±4.21nM/mg Hb)에 비해 유의한 감소를 보였다(p<0.05). 5) 당뇨병성 신경병증 및 HbAlc에 따른 적혈구 항산화 효소 활성도 및 총 glutathion 량은 유의한 변화를 보이지 않았다. 이상의 결과로 조직의 항산화계 변화가 당뇨병 및 당뇨병성 합병증의 발생에 중요한 소인으로 사료되나 상산화계 변화에 대한 원인은 알수 없었다. 따라서 이에 대한 더 많은 연구가 필요하리라 생각된다. Free radical, very strong reactant, may injury to tissue react on the DNA, cell membrane lipid and protein. It was known that free radical may play an important role in the pathogenesis of varying disease such as respiratory tract disease, epidermal carcinoma, psoriasis, rheumatoid arthritis and autoimmune disease, also aging process. We studied to assess the antioxidant status of red cells in human diabetics by measuring activities of cellular antioxidant enzymes(SOD< GSHPX, Catalase), G6PDH and total glutatione level. The result were summarized as follows : 1) The superoxide dismutase altivity of red cells in diabetics (2.204±0.711 U/mg Hb) was significantly lower than in normal control group(3.070±0.721 U/mg Hb) (p<0.05). 2) The glucose 6?phosphate dehydrogenase (G6PDH) activity of red cells in diabetics (0.648±0.241 U/mg Hb) was significantly higher than in normal control group (0.456±0.190 U/mg Hb) (p<0.05). 3) The superoxide dismutase activity of red cells in diabetics over 10 years duration (1.96±0.92 U/mg Hb) was significantly lower than in normal control group (3.07±0.72 U/mg Hb) (p<0.05), also glutathione peroxidase activity of red cells in diabetics over 10 years duration (0.74±0.17 U/mg Hb) was significantly lower than in normal control group (1.81±0.48 U/mg Hb), in diabetics below 5 years duration (1.26±0.38 U/mg Hb) and in diabetics 5 to 10 years duration (1.45±0.42 U/mg Hb) (p<0.01). 4) The catalase activity of red cell in diabetics associated with retinopathy (2.93±0.51 U/mg Hb) was significantly lower than in D.M without retiopathy (4.01±0.28 U/mg Hb) (p<0.01). 5) The glutathione peroxidase activity of red cell in diabetics associated with retinopathy (0.74±0.27 U/mg Hb) was significantly lower than in D.M without retinopathy (1.47±0.54 U/mg Hb) (p<0.05) and in normal control group (1.81±0.48 U/mg Hb) (p<0.01). 6) The glutathione peroxidase activity of red cells in diabetics associated with nephropathy (1.10±0.39 U/mg Hb) was significantly lower than in normal control group (1.81±0.48 U/mg Hb) (p<0.05), also total glutathione levels in D.M with nephropathy (10.61±48 nM/mg Hb) was significanthy lower than in D.M without nephropathy (15.90±4.21 nM/mg Hb) (p<0.05). These results suggest that tissue antioxidant status may be an important factor in the etiology and complication of diabetes mellitus, but it is not clear wheather tissue antioxidant system was affected, and additional study will be needed for a full understanding of the role that free radical play in the antioxidant defense systems.

위궤양 환자에서 Omeprazole 반복 투여시 혈청 Gastrin과 Pepsinogen A치의 변화

전용준,신양수,유병전,최성도,서영환,노상현,박찬국,김만우 大韓消化器病學會 1991 大韓消化器病學會誌 Vol.23 No.4

Paraquat 투여 백서에서 Cyclophosphamide와 Dexamethasone의 치료 효과

신정철,신양수,유병전,임현성,정태흥,박찬국,오금탁,이병래 朝鮮大學校 附設 醫學硏究所 1991 The Medical Journal of Chosun University Vol.16 No.1

Paraquat (N,N'-dimethy 1-4,4'bipyrydinium : Gramoxone^(R), a widely used herbicide, is extremely toxic to all green plants and many eukaryotic organisms. In human, paraquat intoxication leads to multiple organ failure, it preferentially damages the lung, kidney and liver and may result in death. The participation of superoxide in initiating tissue damage by administration of paraquat is well known in which one electron reduction of paraquat leads to the formation of radical species which react with molecular oxygen to give superoxide. This process initiates immunological changes with the activation of neutrophilic leukocytes, which is related to further production of superoxide. In this experiment, therapeutic effects of cyclophosphamide and dexamethasone on blood cell count, malondialdehyde as a indicator of lipid peroxidation, and antioxidant enzymes levels in the lung, liver and kidney of paraquat intoxicated rats were inbestigated. The WBC count were significantly decreased by combined treatment of cyclophosphamide and dexamethasone in paraquat intoxicated rats at 3 and 5 days but RBC count were not changed. The contents of malondialdehyde were significantly decreased in the lung and Kidney by combined treatment at 10 days after paraquat intoxication. The catalase acitvities were markedly increased in the lung and liver of rats at 10 days after paraquat intoxication, and superoxide dismutase activities showed minimal changes in the lung, liver and kidney of rats by combined treatment. These results suggest that paraquat induced tissue damage in rats can be redeced by combined administration of cyclophosphamide and dexamethasone.

유방에 발생한 원발성 악성 림프종 1예

정태홍,정규성,김원민,유병전,정춘해,이미자,전호종 大韓血液學會 1992 大韓血液學會誌 Vol.27 No.2

페이지 그룹 검색 모델 : 음란성 유해 정보 색출 시스템을 위한 인터넷 정보 검색 모델

육현규(Hyun-Gyoo Yook),유병전(Byung-Jeon Yoo),박명순(Myong-Soon Park) 한국정보과학회 1999 정보과학회논문지 : 시스템 및 이론 Vol.26 No.12

월드 와이드 웹(World Wide Web)에 존재하는 음란성 유해 정보는 많은 국가에서 사회적인 문제를 일으키고 있다. 그러나 현재 음란성 유해 정보로부터 미성년자를 보호하는 실효성 있는 방법은 유해 정보 접근 차단 프로그램을 사용하는 방법뿐이다. 유해 정보 접근 차단 프로그램은 기본적으로 음란성 유해 정보를 포함한 유해 정보 주소 목록을 기반으로 사용자의 유해 정보에 대한 접근을 차단하는 방식으로 동작한다. 그런데 대규모 유해 정보 주소 목록의 확보를 위해서는 월드 와이드 웹으로부터 음란성 유해 정보를 자동 색출하는 인터넷 정보 검색 시스템의 일종인 음란성 유해 정보 색출 시스템이 필요하다. 그런데 음란성 유해 정보 색출 시스템은 그 대상이 사람이 아닌 유해 정보 접근 차단 프로그램이기 때문에 일반 인터넷 정보 검색 시스템과는 달리, 대단히 높은 검색 정확성을 유지해야 하고, 유해 정보 접근 차단 프로그램에서 관리가 용이한 검색 목록을 생성해야 하는 요구 사항을 가진다. 본 논문에서는 기존 인터넷 정보 검색 모델이 “문헌”에 대한 잘못된 가정 때문에 위 요구사항을 만족 시키지 못하고 있음을 지적하고, 월드 와이드 웹 상의 문헌에 대한 새로운 정의와 이를 기반으로 위의 요구사항을 만족하는 검색 모델인 페이지 그룹 검색 모델을 제안한다. 또한 다양한 실험과 분석을 통해 제안하는 모델이 기존 인터넷 정보 검색 모델보다 높은 정확성과 빠른 검색 속도, 그리고 유해 정보 접근 차단 프로그램에서의 관리가 용이한 검색 목록을 생성함을 보인다. Illegal and Harmful Content on the Internet, especially content for adults causes a social problem in many countries To protect children from harmful content, A filtering software, which blocks user's access to harmful content based on a blocking list, and harmful content search system, which is a special purpose internet search system to generate the blocking list, are necessary We found that current internet search models do not satisfy the requirements of the harmful content search system: high accuracy in document analysis, fast search time, and low overhead in the filtering software. In this paper we point out these problems are caused by a mistake in a document definition of the current internet models and propose a new internet search model, Page Group Search Model. This model considers a document as a set of pages that are made for one subject. We suggest a Group Construction algorithm and a Group Evaluation algorithm. And we perform experiments to prove that Page Group Search Model satisfies the requirements.

함암요법으로 골수기능이 억제된 악성종양환자에서 rhG-CSF의 임상적 효과

김원민,서영환,조경상,유병전,김상도,이승일,정춘해 朝鮮大學校 附設 醫學硏究所 1992 The Medical Journal of Chosun University Vol.17 No.2

We have studied the efficacy of rhG-CSF in patients with non-hodgkin's lymphoma, acute leukemia, and small cell lung cancer undergoing anticancer chemotherapy. These patients were below leukocyte count 3,000 cubic millimeter due to myelosuppression induced by the first cycle of intensive chemotherapy. Treatment with rhG-CSF (100㎍ per square meter of body surface area per day in a 30-minute intravenous infusion) was begun two days and for 14 consecutive days after the end of the second cycle of chemotherapy. The results were as follows. 1. The onset of myelosuppression was 6 days after chemotherapy, and the onset of recovery was 16.7 days after chemotherapy, and the duration of granulocytopenia was 10.7 days in patients with malignant tumor during contrast period. 2. The duration of granulocytopenia was shortened 5.2 days in patients administered rhG-CSF than without rhG-CSF, and we observed the shortest duration of granulocytopenia with increasing granulocyte in patients with small cell lung cancer. 3. Observing the differential count of leukocyte checked the highest level of leukocyte in contrast and rhG-CSF period, the persentage of neutrophil was increased in patients with acute leukemia and small cell lung cancer during rhG-CSF period, but not increased in patients with non-hodgkin's lymphoma. 4. rhG-CSF was not influenced on liver faction, renal fuction, uric acid, and glucose metabolism, also had no effect on recovery of platelet. In conclusion, rhG-CSF can be administered to patients with hematologic or nonhematologic malignant tumor that myelosuppression induced by anticancer chemotherapy result in shortening the duration of granulocytopenia and increasing the peripheral neutrophil, therefore full dose chemotherapy can be administered on time, and rhG-CSF may reduce the morbidity and mortality of patients with malignant tumor undergoing chemotherapy.

위장관 ( 胃腸管 ) : 위궤양 환자에서 Omeprazole 반복 투여시 혈청 Gastrin과 Pepsinogen A치의 변화

김만우(Man Woo Kim),박찬국(Chan Gook Park),전용준(Yong Jun Jeon),신양수(Yang Su Shin),유병전(Byung Jeon Yuh),최성도(Sung Do Choi),서영환(Yong Hwan Suh),노상현(Sang Hyun Noh) 대한소화기학회 1991 대한소화기학회지 Vol.23 No.4

N/A The fasting serum gasrin and pepsinogen A levels on oral Omeprazole treatment were observed in 17 patients of gastric ulcer diagnosed by gastrofiberscopy. The fasting serum gastrin and pepsinogen A levels 1 day prior to oral omeprazole treatment, during treatment (1st, 7th, 14th day) and fo]lowing 2 weeks treatment (1st, 7th, 15th day) were monitored respectively and compared each other. The results were as follows. 1) Serum gastrin and pepsinogen A levels during and following the treatment were significantly increased compared to basal gastrin and pepsinogen A levels before treatment each (p<0.01). 2) Serum gastrin levels following the 2 weeks treatment did not change significantly compared to that during treatment. 3) The relationship between the basal serum gastrin levels before treatment and the gasrin levels during the treatment showed positive correlation (r=0.414: p<0.01).