Chiral Separation of (±)-Higenamine by Capillary Electophoresis

Choi,One-Kyun,Jung,Kyo-Soon,Choi,Heisook-Yun,Yang,Deok-Chun 한국자원식물학회 2003 Plant Resources Vol.6 No.1

Higenamine [1-(4-hydroxy-6, 7-dihydroxy-l, 2, 3, 4-tetrahydroisoquinoline) is a cardiotonic constituent of Aconiti tuber, one of the most widely prescribed oriental medicines. S-(-)higenamine was reported to have a stronger cardiotonic activity than R-(+)-higenamine and known as a central intermediate in the biosynthesis of various benzyl isoquionoline alkaloids in plants. The separation of higenamine enantiomers has been accomplished with capillary electrophoresis using cyclodextrins (CDs) as chiral selectors. Good resolution of this enantiomers was obtained using a 50 mM sodium phosphate buffer containing hydroxypropyl β-CDs using 27 cm fused silica capillary (50㎛ i.d., 20 cm to detector) at 25℃. With the electric field of 340 V/cm, the separation time of higenamine enantiomers was less than 6 min. Under this optimum conditions, the relative standard deviations of migration time and peak area were less than 1.6% and 3.2%. A 512-channel diode array detector was confirmed for the higenamine. The detection limits (S/N = 3) of these enantiomers are 1.5mutextrmm/mL. We confirmed the chiral form of higenamine in medicinal plants.

복막투석복막염 환자에서 발생한 창자벽공기증 1예

정선영,나지훈,최윤정,고성애,조규향,박종원,도준영,윤경우 영남대학교 의과대학 2009 Yeungnam University Journal of Medicine Vol.26 No.1

Peritonitis is a serious problem in patients undergoing peritoneal dialysis. Rarely pneumatosis intestinalis can occur as a complication of this infectious process. Pneumatosis intestinalis is a potential life threatening condition with a challenging management. The mortality of peritoneal dialysis patients with pneumatosis intestinalis secondary to mesenteric ischemia is almost 100%. We describe a rare case of pneumatosis intestinalis in a peritoneal dialysis patient who developed Staphylococcus aureus peritonitis which was initially treated with appropriate antibiotics. Since initial response to therapy was not achieved, an abdominal computerized tomography was done which revealed a pneumatosis intestinalis. A laparotomy was performed and small bowel necrosis was seen. A segmented resection with ileostomy, jejunostomy was done. Though surgical treatment was performed, the patient died in 2 weeks after admission. Pneumocystitis intestinalis in peritoneal dialysis peritonitis is a uncommon complication which requires prompt evaluation to rule out mesenteric ischemia as it carries a high mortality and its management will be surgical.

식민지 이후의 탈식민주의 -설정식 시를 중심으로-

최윤정 ( Yun Jung Choi ) 한민족문화학회 2012 한민족문화연구 Vol.39 No.-

설정식은 한때 월북문인이라는 표식에 갇혀 삭제되거나 좌익적 경향의 시인으로 리얼리즘이나 문학가동맹과 연계되어 민족의 현실을 간과하지 않았다는 사실만으로 평가되었다. 그의 시론으로부터 혁명적 낭만주의 경향을 포착하게 되는데, 이는 민족주의적 현실인식의 관점이 제공되는 계기를 드러낸다. 우리는 설정식이 해방공간의 현실을 깊게 들여다보고 당시의 좌우 이데올로기에 편향되기 보다는 그 안에 내포되어 있는 제국주의를 간파하고 두 진영으로부터 거리를 두려했던 민족주의자로 파악할 수 있게 되는 것이다. 우선 우편향적 미국에 대해서는 ``인민``이라는 계급이데올로기를 표방함으로써 미국에 결탁한 보수세력들과 미국적 제국주의와 거리를 두게 되고, 그 ``인민``이 혈통적 단위임을 피력함으로써 계급적으로 구체화되는 존재가 아니라 민족적 주체들임을 제기하고 민족을 무시하는 국제주의 이데올로기를 표방하는 소련적 사회주의와도 거리를 두게 된다. 또한 ``자유``라는 민주주의의 이상이 파괴되는 현실은 미국의 식량정책으로 표면화된다. 쌀 기근 사태는 아직도 이 땅에서 나는 곡식의 주인이 될 수 없는 현실을 드러내며, 식량정책을 주도하는 미국과 거리를 두게 하며, 추상적인 사상과 이데올로기를 표방하는 ``빵``과 대비됨으로써 민족의 현실을 구체화하게 된다. 이렇게 2장에서는 좌우이데올로기로부터 등거리를 유지하면서 거기에 내포된 제국주의를 간파하게 되고, 민족주의적 경향을 뚜렷하게 드러내는 과정을 통해 ``인민``, ``자유``, ``민족``이 설정식 시텍스트의 키워드임을 발견한다. 3장에서는 ``인민``과 ``자유``, ``민족``을 드러내는 대립적 상징체를 통해 민족담론을 구체화하게 된다. ``인민``은 2장에서 혈통적 민족임이 드러난다. ``인민``의 상징체인 ``해바라기``는 제국의 상징체인 ``태양``을 필적하는 존재로 파악된다. ``자유``는 2장에서 민족적 속박의 지속성을 환기시키는데, 제국에 의해 민족이 분열된 영토는 ``집``으로 표기되고 제국이 도모하지 못하는 민족의 절대域을 ``숲``으로 대비함으로써 구속과 예속으로부터의 탈주를 통해 민족적 ``자유``를 예고한다. 또한 ``팔월``이라는 민족의 기념비적 시간을 통해서는 해방이 되었으나 아직 해방이 되지 않은 두 가지 현실 사이에 민족이 놓여 있음을 통해 진정한 해방이 되어야 함을 당위적으로 설파하게 된다. The time of liberation from Japaness imperialism that we are beyond the point we are met with the United States and the Soviet Union is a branch of a new empire. The japanophilism has not been liquidation and our nation stood at the crossroads. Our situation was anxiety in the time. Because A mix of colonialism and postcolonial hold, was present between the division and independence. In various trend, Seol Jeong-sik is only one configuration of the discourse. It was not yet complete will be liberated. The only one empire has been removed by another empire. Even after liberation, our colonial life was being sustained. This discourse is constantly activated, he is discarded pseudo-ethnic discourse divide into left-wing and right-wing ideology. And he presents nation discourse consists of the bottom. He was fully aware of the postcolonial. It is national independence and people`s independence is to be done with. In fact, the important problem was the restoration of nation territory. His anti-imperialist lines does not permit any empire. So he placed a distance equal to the Soviet Union and the United States. In this perspective, he will be close to nationalist rather than proletarian.

크론병에 대한 CoPPLX의 방어효과에 대한 연구

이호학,김유림,문창기,이재일,양병윤,김종수,이정민,정재혁,안석진,박상준,김윤권,김소연,김영중,조민구,최영자,장성조 圓光大學校 醫科學硏究所 2005 圓光醫科學 Vol.20 No.1

1-Phenylindole Ester류의 합성과 반응성 연구

崔舜圭,金兪瑛,兪丙國,李龍均,鄭大一 東亞大學校附設基礎科學硏究所 1993 基礎科學硏究論文集 Vol.10 No.1

일반적인 dienophile에 대한 pyrrole의 반응은 pyrrole이 diene system으로 작용된 [4+2] 고리부가반응과 pyrrole의 α-탄소 위치에 부가되는 Michael형태의 반응이 알려져 있다.

황산이온 포착용 보호컬럼을 가진 이온 크로마토그래피에 의한 선택적인 음이온의 동시검출

최용욱,김종훈,김미경,최윤정,이수영 전주대학교 자연과학종합연구소 1997 전주대학교 자연과학연구소 학술논문집 Vol.10 No.3

Simultaneous detection method for measuring anions in drinking water except sulfate ion was developed by suppressed ion chromatography. Eluent with existing composition between carbonate and bicarbonate made the separation of early eluting anions poor. Therefore, it is necessary to use weaker eluent to increase the resolution of them. However, this caused elution time of sulfate ion to take long, consquantely, total pinning time was more than 60 minutes. In order to resolve this problem by use of column switching with guard column for trapping sulfate ion, the resolution of the rest 8 anions was enhanced, total running time was also shortened within 10 minutes. Usage of eluent with existing composition made possible determination of sulfate ion on guard column within 4 minutes. Calibration curves of 8 anions represented linearity of better than 0.999 and detection limits by this method of all the anions considered were between 0.04 and 0.002 mg/L

성장기 쥐에서 이소플라본 첨가수준이 골밀도 및 골함량에 미치는 영향

최미자,정윤정 啓明大學校 生活科學硏究所 2005 科學論集 Vol.31 No.-

The purpose of this study was to examine the effects of isoflavones supplementation on bone mineral density and bone mineral content in growing female Sprague-Dawley rats. Twenty-seven rats were divided into three groups casein diet, casein+1/2IF(1.7 mg isoflavones/g protein)diet, and casein+IF(3.4mg isoflavones/g protein) diet. All rats has been fed on experimental diet and deionized water ad libitum for 9 weeks. Bone mineral density(BMD) and bone mineral content(BMC) were measured using PIXImus(GE Lunar Co, Wisconsin, USA.) in spine and femur on 9 weeks after feeding. Diet did not affect weight gain and mean food intake. Food efficiency ratio was lower in casein +IF groups. The casein+IF and casein+1/2IF group had significantly higher spine and femur bone mineral density than the casein group. We found that bone mineral density effected by isoflavones supplementation has been resulted differently in accordance with the type of bone. Therefore, isoflavones supplementation may be beneficial on spine and femur BMD increasement in growing female rats.

한의진단명과 진단요건의 표준화 연구 III : 3차년도 연구결과 보고

최선미,양기상,최승훈,박경모,박종현,심범상,김성우,노석선,이인선,정진홍,이진용,김달래,임형호,김윤범,박성식,송태원,김종우,이승기,최윤정,신순식 한국한의학연구원 1997 한국한의학연구원논문집 Vol.3 No.1

The diagnostic requirements were suggested and explained regarding the systems of differentiation of symptoms and signs in the third year study of standardization and unification of the terms and conditions used for diagnosis in oriental medicine. The systems were as follows : - analyzing and differentiating of epidemic febrile disease - analyzing and differentiating in accordance with the Sasang constitution medicine based on four-type recognition - differentiation of disease according to pathological changes of Chong and Ren channels - standards for diagnosis of women's disease - standards for diagnosis of children's disease - standards for diagnosis of motor and sensor disturbance (-muscle. born, joint, etc.) - standards for diagnosis of neuropsychiatric disease - standards for diagnosis of five sense organ disease - standards for diagnosis of external disease The indivisual diagnosis pattern was arranged by the diagnostic requirements in the following order : another name, notion of diagnosis pattern, index of differentiation of symptoms and signs, the main point of diagnosis, analysis of diagnosis pattern, discrimination of diagnosis pattern, prognosis, a way of curing a disease, prescription, herbs in common use, disease appearing the diagnosis pattern, documents. The standards for diagnosis of each disease was arranged by the diagnostic requirements in the following order : another name, notion of disease, the main point of diagnosis, analyzing and differentiating of disease, analysis of disease, discrimination of disease, prognosis, a way of curing and prescription of disease, disease in western medicine appearing the disease in oriental medicine, documents.

석류 과피 추출물의 라디칼 소거활성

최윤정,오영지,정동선 서울여자대학교 2010 자연과학연구논문집 Vol.22 No.-

Antioxidant activity and total phenolic contents of Punica granatum peels were investigated. The pomegranate peels were extracted with 70% methanol (ME) and then partitioned to hexane (HF), ethyl acetate (EAF) and water (WF) fractions. Total phenolic contents and radical scavenging activities of each extract and fraction from Punica granatum peels were measured. The ethyl acetate fraction showed the highest radical scavenging activity, followed by methanol extract, water fraction and hexane fraction. The IC50 values of EAF were 0.144 ㎍./mL and 0.50 ㎍./mL for DPPH and ABTS radicals scavenging activities, respectively. A strong antioxidant activity of the EAF from pomegranate peels was correlated with its high level of total phenolic contents (740.0 mg of tannic acid equivalent/g). ME and WF also exhibited relatively strong radical scavenging activities with IC50 values of 6.70 ㎍./mL and 7.25 ㎍./mL for DPPH radical, and 17.4 ㎍./mL and 18.33 ㎍./mL for ABTS radical, respectively. These results suggest that the pomegranate peels possess strong antioxidant activity, and can be a useful source of natural antioxidant.

형광 검출 흐름 주입 분석법에 의한 암모늄 이온의 선택적 정량

최용욱,김미경,최윤정 전주대학교 자연과학종합연구소 1997 전주대학교 자연과학연구소 학술논문집 Vol.10 No.3

Selective determination of aqueous ammonia by flow injection analysis with fluorometric detection was described. The length of the mixing coil and the reaction coil, the composition of organic solvent, the sulfite concentration, the pH of buffer solution and the reaction temperature were optimized as analytical parameters for selectively determining NH_4^+/NH_3 by using FIA setup. The limit of detection (LOD) and the repeatability for aqueous ammonia were 0.04 ㎍/L (3SD) and better than 1%, respectively. This analytical condition showed considerable selectivity for aqueous ammonia over amino acids studied. Examples of the use of this FIA system were given for the anlysis of aqueous ammonia in lab water, tap water and groundwater.