在學生 實態調査

安秉基,申大鉉 충남대학교 학생생활연구소 1972 學生生活硏究 Vol.1 No.-

RE-09 : High mobility group box-1 and Toll like receptor 4 in Endometriosis: Mediating NF-kB pathway

( Bo Hyon1 ),( Yun Ji Jung ),( Seung Joo Chon ),( Yoo Jin Lee ),( E Jung Han ),( Sihyun Cho ),( Young Sik Choi ),( Byung Seok Lee ),( Seok Kyo Seo ) 대한산부인과학회 2014 대한산부인과학회 학술대회 Vol.100 No.-

목적: The aim of the study was to examine the sterile, chronic inflammatory status in endometriosis by detecting High mobility group box-1 (HMGB-1), and HMGB-1 signaling through toll like receptor (TLR) 4 in endometrial stromal cell, which activates NF-kB pathway that might play a pathogenic role in endometriosis. 방법: Total 69 patients who had undergone hysterectomy from March, 2012 to March, 2014 were included: 39 patients who had endometriosis as case group, 30 participants without endometriosis as the control. Expression of HMGB-1 in endometrium was examined by immunohistochemistry. From endometriosis patient, eutopic endometrium was obtained and used to culture in vitro. To confirm the association of HMGB-1 and TLR4 in NF-kB pathway, the cultured human endometrial stromal cells (HESCs) were treated with recombinant HMGB-1 (15 ng/mL) for 0, 12, 24, 48 hrs. TLR4 antagonist (LPS-RS) was added after rHMGB-1 treatment to HESCs to see the change of proliferation after inhibiting TLR4. CCK8 proliferation assay kit. Real-time PCR and western blotting were used to examine the proliferation, quantification of the mRNA and protein. Specific inhibitor of NF-κB signaling pathway (TPCA-1) was used to explore the role of NF-κB signaling pathway in HESCs proliferation. 결과: HMGB-1 expression was significantly higher in glandular epithelial and stromal cells of endometriosis group compared to the controls. rHMGB-1 significantly enhanced cell proliferation in HESCs and increased TLR4 expression by dose dependent fashion. Inhibiting TLR4 by LPS-RS, cell proliferation in HESCs and expression of TLR4 were significantly decreased comparing to the controls. After blocking NF-kB pathway with TPCA-1, cell proliferation in HESCs was significantly decreased by dose dependent fashion. 결론: This study showed that HMGB-1 may play an important role through TLR4 and NF-kB pathway, in inflammatory cascade of pathogenesis in endometriosis.

2003년도에 추가된 고추 유전자원의 역병에 대한 저항성

심현우,이진용,김정훈,김병수 慶北大學校 農業科學技術硏究所 2004 慶北大農學誌 Vol.22 No.-

Eighty five accessions of our laboratory pepper germplasm collections in 2003 were tested for resistance to Phytophthora capsici Leonian. Any new material highly resistant was not found among them. However, KC1184, which has horticultural characteristics of Korean peppers, was moderately resistant and appeared worthwhile in breeding for resistance.

실존주의 사상과 현대 영미문학 : 현대 실존 해석학과 포스트모던 문학까지

이상혁,양병현,최진영,현중식 中央大學校 人文科學硏究所 1998 人文學硏究 Vol.27 No.-

NITINOL을 이용한 차동식 액츄에이터의 설계에 관한 연구

정상화,김현욱,신현성,차경래,신병수 한국공작기계학회 2002 한국공작기계학회 춘계학술대회논문집 Vol.2002 No.-

In the recent years, as the research and the development of micro and precision machinery become active, the interest of micro actuators using SMA (Shape Memory Alloy) has been increased. The dynamic characteristic analysis of SMA is necessary for actuator application and many common researches report the material characteristics of SMA sufficiently. However, the research on dynamic characteristics is very deficient. In this paper, the helical spring are fabricated with NiTi SMA wire of high resistivity. The force, response speed, temperature, and displacement are measured by digital force gauge, infrared thermometer, and laser displacement sensor so that the dynamic characteristics of this SMA is analyzed. Also, bidirectional actuator was fabricated and experimented for its performance.

서울의 Penicillinase Producing Neisseria Gonorrhoeae 발생빈도(1996)

김재홍,황동규,전재홍,김윤석,김중환,김용준,이창균,임동진,김현수,조창근,김경문,박상훈,전우형,김희성,이호정,차명수,김갑형,김형석,김석우,황지환,박병순,권오상,이민수,송기훈,성소영,이인섭,부태성 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.2

Background : In recent years, gonorrhea has been panedemic and remains one of the most commom STDs in the world, especially in developing countries. Objective & Methods: For the detection of a more effective therapeutic regimen and assessing the prevalence of PPNG, we have been trying to study the patients who have visited the VD Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results: In 1996, 139 strains of N. gonorrhoeae were isolated, among which 53(39.0%) were PPNG. Conclusion: Our results suggests that after a peak of 74.3% in 1993, the prevalence of PPNG in Seoul is gradually declining.

급성 외과적 복증으로 오인된 호산구성 위장관염 3예

선병호(Byung Ho Sun),김철우(Chul Woo Kim),박진현(Chin Hyon Park),이병철(Byung Chul Lee) 대한외과학회 1985 Annals of Surgical Treatment and Research(ASRT) Vol.28 No.6

S-711 Case Report: Hemophagocytic Syndrome in a Patient with Dermatomyositis

( Byung Wook Song ),( So-yeon Lee ),( Ji Min Lee ),( Hye-jin Jeong ),( Chang-nam Son ),( Hye Ra Jung ),( Sang-hyon Kim ),( Ji-min Kim ) 대한내과학회 2016 대한내과학회 추계학술대회 Vol.2016 No.1

Introduction:?Dermatomyositis (DM) is characterized by chronic inflammation of striated muscle and characteristic cutaneous manifestations. Hemophagocytic syndrome (HPS) is a rare life-threatening condition caused by uncontrolled activation of histiocytes resulting in prominent hemophagocytosis. Particularly, occurrence of HPS in the patients with DM is extremely rare. We report the first case of HPS in patient with DM successfully treated in Korea.?Case:?A 56-year-old female visited our hospital, complaining of general weakness with whole body skin rash for 2 months. On physical examination, she had symmetric proximal muscle weakness (motor power grade 1/5 in both upper and lower extremities) and characteristic skin lesions including heliotrope rash, gottron’s papules and V sign. She also had a swallowing difficulty proven by abnormal videofluoroscopic swallowing test. Laboratory findings showed thrombocytopenia, elevated serum levels of muscle enzymes (creatine kinase, myoglobin, and lactate dehydrogenase), ferritin and triglyceride. Although antinuclear antibody was positive (1:160), anti Jo-1 antibody was negative. We identified the inflammatory myopathy through electromyography and performed a biopsy of inflamed muscle on magnetic resonance image. Based on these data, she was diagnosed with DM. We also performed bone marrow biopsy to find the cause of a microangiopathic hemolytic anemia and thrombocytopenia in peripheral blood smear. Numerous CD68-positive macrophages engulfing erythrocytes and platelets were revealed in bone marrow study. She was finally diagnosed as DM with secondary HPS. After steroid pulse therapy (methylprednisolone 1 g/day) for 3 days was initiated, we continued high dose steroid therapy for 1 month. After then, we gradually tapered the steroid and started methotrexate. After 1 year of treatment, she was completely recovered from muscle weakness, swallowing difficulty, skin lesions and cytopenia without any other sequelae.?Conclusions:?With this unique case, we would like to assert that HPS should be considered when cytopenia is observed in the patients with DM and that early aggressive therapy is needed.

Pathology-based Evaluation of Chemical Toxicity in the National Toxicology Program and Development of Toxicological Testing Methods for Human Risk Assessment : Carcinogenicity Studies of Dioxin and Dioxin-like Chemicals

Byung-IL Yoon,Yongbaek Kim,Beom Seok Han,Boo-Hyon Kang 한국실험동물학회 2009 Laboratory Animal Research Vol.25 No.4

본 연구에서는 NTP에서 수행한 다이옥신 및 다이옥신류 화학물질들의 발암성시험으로부터 얻어진 병리결과를 토대로 어떠한 기준과 근거에 의해서 발암성을 정의하는 지에 대하여 예를 들어 설명하였다. 그러나, 본문에서도 언급하였듯이 동물실험에서 발암성이 음성으로 나왔다고 해서 발암물질이 아니라는 의미가 아니며, 발암성이 있다고 하는 것은 해당물질이 사람에 노출되면 종양발생과 관련하여 해로울 수 있다는 것을 나타낸다. NTP에서는 병리결과에 근거하여 5가지의 분류기준에 따라 시험물질들의 발암성을 평가하고 있으나, 보다 신뢰성 있는 결론을 도출하기 위하여 물질의 독성기전에 기초한 실험들을 발암성시험에서 함께 병행하고 있다. 다이옥신과 유사물질들의 독성이 AhR의 매개로 일어나며, CYP1A1과 CYP1A2 간대사효소의 발현과 깊이 연관이 있기 때문에 발암성시험기간 동안 효소의 활성도를 함께 측정하였으며, 간세포 증식성 검사, 갑상샘호르몬 농도측정도 수행하였다. 즉, 발암성평가시험의 경우 일반적으로 병리검사결과가 판단의 중요한 역할을 하지만, 물질의 작용기전에 기초한 가능한 많은 정보를 획득하여 분석함으로써 보다 올바르고 정확한 결론을 내릴 수 있도록 노력해야 한다. 발암성평가는 병리결과에 근거하여 5가지의 판단기준에 따라 발암성을 분류하는데, 발암성시험에 있어 결론을 내릴 때 고려해야 할 중요한 사항들을 요약하면, 1) 실험의 계획과 수행이 잘 이루어졌는가, 2) 자연적으로도 자주 관찰되는 종양과 드문 종양의 발생, 3) 양성종양에서 악성종양으로, 전암병변에서 종양성병변으로의 진행된다는 증거 또는 이론이 있는가, 4) 일부 양성종양은 퇴축되는 반면, 동일한 형태학적 특성을 가진 일부 양성종양은 악성으로 진행되는데, 형태학적으로 이것을 구분하는 것은 불가능하다. 따라서, 양성이 악성으로 진행될 수 있는 가능성이 있다고 한다면 양성종양이 악성종양으로 진행된다고 가정한다. 5) 동일한 장기 또는 조직에서 진행의 연속성을 가지는 경우 양성과 악성종양의 발생을 합하는 것, 즉, 양성과 악성을 따로 떼어 보면, 대조군과 별 차이가 없어 보이지만, 합하게 되면 뚜렷한 증가추세와 통계적으로도 유의차가 나타나는 경우가 있다. 6) 특정부위에서 발생한 종양의 multiplicity, 7) 전이(metastasis), 8) 종양이 발생한 동일한 장기에서의 과형성병변의 발생, 또는 다른 종 또는 성에서 수행한 실험에서 나타난 과형성병변의 발생양상, 9) 용량상관성 여부, 10) 발생한 종양의 통계적으로 유의차 있는 증가, 11) 특정 종양의 대조군에서의 발생정도와 종양발생정도의 개체차이, 12) 생존률을 고려한 분석과 의양성(false positive) 및 의음성(false negative) 가능성, 13) 시험물질의 화학적 구조와 활성과의 상호관련성(structure-activity correlation), 14) 유전독성여부(genetic toxicity) 등이다. NTP 시험결과보고서는 내용이 광범위하고 많아 본 연구에서 모든 것을 다룰 수는 없었으나, 병리결과를 토대로 다이옥신과 유사물질들의 발암성평가결과를 해석하고 평가하는 기준과 근거들을 예를 들어 설명 고찰하였다. Pathological data sets are critical for evaluation of carcinogenicity of chemicals in the preclinical studies using laboratory animals. Proper diagnosis and interpretation of non-neoplastic and neoplastic lesions, no matter what they are spontaneously developed or chemically induced, are prerequisite for determination of chemical carcinogenicity. The pathology-based criteria have been developed to evaluate the carcinogenic activity of chemicals; two positive results (clear evidence and some evidence), one uncertain finding (equivocal evidence), no observable effect (no evidence), and inadequate study. However, there are lots of things that we need to consider before determination of carcinogenic activity of chemicals such as adequacy of the experimental design and conduct, common and uncommon neoplasia, progression from benign to malignant neoplasia as well as from preneoplastic to neoplastic lesions, combining benign and malignant tumor incidence in the same organs, multiplicity in site-specific neoplasia, dose relationship, statistical significance of the observed tumor increase, concurrent control tumor incidences as well as historical control rate for a specific neoplasm, etc. In the present study, we introduce the carcinogenicity studies of dioxin and dioxin-like chemicals which had been performed as National Toxicology Program (NTP) in the USA for years, and discuss the pathology data-based approaching strategy for determination of carcinogenic activity of chemicals.

P-5. A Pathologic Observation on Spontaneous Mammary Tumors in Female Sprague-Dawley Rats

Byung Il Yeoun,Chang-Hyeong Lim,Boo-Hyon Kang 한국실험동물학회 1993 한국실험동물학회 학술발표대회 논문집 Vol.1993 No.-