국내 분리 토끼출혈병 바이러스(RHDVa)를 감염시킨 토끼 간장에서의 경시적인 초미세구조 변화와 apoptosis

박중원,전지은,박은정,김한,이명헌,황의경,김재훈,이중복,우계형,Park, Jung-Won,Chun, Ji-Eun,Bak, Eun-Jung,Kim, Han,Lee, Myeong-Heon,Hwang, Eui-Kyung,Kim, Jae-Hoon,Lee, Chung-Bok,Woo, Gye-Hyeong 대한수의학회 2013 大韓獸醫學會誌 Vol.53 No.1

In this study, to understand the pathogenesis of new rabbit hemorrhagic disease virus (RHDVa) serotype, we carried out to administrate RHDVa to rabbits, and to examine sequential electron microscopic changes and relationship between pathogenesis and apoptosis. TUNEL-positive cells began to be observed from 24 hours after inoculation (HAI) and the number of positive cells was slightly increased with the course of time. Whereas marked increase of positive cells was seen in the liver from the rabbits died acutely. Typical viral particles with cup-like projections and a diameter of 30~40 nm were detected in homogenized liver samples and tissues at 36 and 48, and 48 HAI, respectively. Ultrastructurally, glycogen deposition was observed from the first stage of hepatocellular degeneration by RHDVa infection and then, swelling and disruption of cristae of mitochondria by viral particles, swelling of smooth endoplasmic reticulum, vacuoles and vesicles were detected. Condensation, margination and fragmentation of chromatin were observed in degenerative hepatocytes at 36 and 48 HAI, indicating apoptotic bodies. These data offer that hepatocytic apoptosis by RHDV infection could be closely related with mitochondrial impairment in the hepatocytes.

새로운 국내 분리 토끼출혈병바이러스(RHDVa)를 감염시킨 토끼에서의 경시적인 병리학적 변화와 조직 내 바이러스 항원 분포

박중원,전지은,양동군,박은정,김한,이명헌,황의경,이중복,우계형,Park, Jung-Won,Chun, Ji-Eun,Yang, Dong-Kun,Bak, Eun-Jung,Kim, Han,Lee, Myeong-Heon,Hwang, Eui-Kyung,Lee, Chung-Bok,Woo, Gye-Hyeong 대한수의학회 2012 大韓獸醫學會誌 Vol.52 No.2

Rabbit hemorrhagic disease is a highly acute and fatal viral disease caused by rabbit hemorrhagic disease virus (RHDV). Since first outbreak in Korea 1987, RHDV has been continually affected in the country, but the pattern of outbreak seem to be changed. In this study, to understand the pathogenesis of the new RHDVa serotype, we therefore carried out to inoculate RHDVa to rabbits, and to examine the sequential histopathologic changes and viral distribution. Macroscopically, various sized dark red or white spots or appearance were observed in the liver, lung, kidney uterus and ureter. In euhanized rabbits, significant pathologic findings such as infiltration of heterophils and mononuclear cells were observed at 24 hours after inoculation (HAI), and these were sequentially extended periportal to centrilobular area. However, in dead rabbits, severe hepatic degeneration and/or necrosis with relatively weak inflammatory responses were observed. RHDV antigens began to detect in liver, spleen, and lung from 12 HAI by PCR. Immunohistochemically, RHDV positive cells were seen in only liver from 24 HAI, and the degree of immunogen reactivity was stronger in dead rabbits than in euthanized ones. In conclusion, RHDVa caused the subacute or chronic infection accompanying low mortality and moderate to severe inflammatory reaction in rabbits, suggesting the possibility that RHD could become endemic.

SV 40 Large T 유전자를 이용해 불멸화시킨 사람 태아 간세포주의 확립

박중원(Joong Won Park),이주영(Joo Young Lee),이효석(Hyo Suk Lee),박주배(Joo Bae Park),김정룡(Chung Yong Kim) 대한내과학회 1994 대한내과학회지 Vol.46 No.4

N/A Background: Hepatocyte culture represents a cell model for analyzing the mechanism involved in car- cinogenesis of carcinogens, a tool for measuring hepatotoxicity of drugs and a simple model for studying hepatitis virus life cycle. However, problems raised by both the short-term survival and the poor functional stability of hepatocytes in culture hindered scientist in using of this in vitro system. Thus we planed to obtain the immortalized and differentiated human hepatocyte cell line by modifying the genome by transfecting the cells with viral specific DNA, always available in studying the liver disease. Method: After primary culture of hepatocytes obtained by therapeutic abortion at 18 weeks of gestation, SV40 large T gene was transfected into the cells by using Polybren-DMSO method, And then transfected hepatocytes were selected in G418 containing medium. Selected, transformed hepatocytes were subcultured in 10% fetal bovine serum containg F-12 medium. Morphological characteristics of subcultured cells was followed by phase-contrast microscope and electoron microscope. The immunocytochemisty using anti-human albumin and anti-human alpha fetoprotein and the immunofluorescence using snti-SV40 T antigen were performed for proving the differentiation of sub-cultured hepatocytes. Results: Electron microscope revealed subcultured cell to be epithelial cell. After more than 20 passages over a period of 7 months, the cells retained an epitheloid morphology. All the SV40 transformed hepatocyte cell lines were 100% positive for T antigen. Significant anti-alpha fetoprotein staining and week anti-albumin staining were observed in cytoplasm around nucleus and so we confirmed the synthesis of liver specific protein of transformed hepatocytes, Conclusion: Human fetal immortalized hepatocytes cell line secreting the liver specific proteins was established.

B형 간염바이러스 표면항원과 항체가 동시에 발현된 만성 간염 환자에서 표면항원 `a` 결정기 유전자의 변이

박중원(Joong won Park),윤정환(Jung Hwan Yoon),황유진(You Jin Hwang),이효석(Hyo Suk Lee),김정룡(Chung Yong Kim) 대한소화기학회 1997 대한소화기학회지 Vol.29 No.2

N/A Background/Aims: A humoral escape mutant of hepatitis B virus(HBV) having mutations at HBsAg a' determinant has been found in patients with concurrent HBsAg and anti-HBs in sera. This study was undertaken to investigate whether such a variant was involved in Korean patients with chronic hepatitis B, Methods: We conducted an analysis of sequences of DNA encoding the HBsAg a' determinant in six chronic hepatitis B patients with concurrent HBsAg an6 anti-HBs positivity, and in three HBsAg-positive controls without anti-HBs in sera. One of the six cases 4ad received long-term hepatitis B immunoglobulin treatment after liver transplantation and five were chronic hepatitis B patients with naturally occurring concurrent HBsAg and anti-HBs. HBV DNA extracted from sera of nine patients was amplified and sequenced within the S gene encoding the a' determinant. Results: HBVs from three controls had no rnutations in the a determinant. Six patients with concurrent HBsAg and anti-HBs had point mutations in the S gene encoding the a' determinant. A liver-transplanted patient had substitutions at nucleotide 587(arginine for glycine at aa 143) and nucleotide 561(tyrosine for serine at aa 136) of the S gene. All of the five patients with naturally occurring concurrent HBsAg and anti-HBs had a substitution at nucleotide 531(serine for isoleucine at aa 126) and four of five had substitutions at nucleoti<le 546 and 552(asparagine for threonine at aa 131 and threonine for methionine at aa 133). One case had three consecutive substitutions at nucleotide 554, 555, 556(arginine for phenylalanine at aa 134). Conclusions: These results demonstrated the presence of mutations at HBsAg a detenninant in Korean chronic hepatitis B patients with concurrent HBsAg and anti-HBs. (Korean J (astroente- rol 1997;29:182 - 191)

안와종야의 분류 및 치험

박중원(Park Jung Won),정화선(Wha Sun Chung) 대한안과학회 1997 대한안과학회지 Vol.38 No.8

소침습적 관상동맥우회술

나찬영,이영탁,박중원,정도현,정일상,정윤섭,김욱성,방정현,이섭,정철현,김웅한,박영관,김종환,홍승록,한재진,이건,Na, Chan-Young,Lee, Young-Tak,Park. Joong-Won,Chung, Do-Hyun,Jung, Ill-Sang,Jung, Yoon-Seup,Kim, Ok-Sung,Bang, Jung-Hyun,Lee, Sub,Chung, C 대한흉부심장혈관외과학회 1998 Journal of Chest Surgery (J Chest Surg) Vol.31 No.2

관상동맥우회술은 인공심폐기 및 심근보호의 안정성으로 인공심폐기 사용하에 시행하는 것이 보편화된 방법이다. 그러나 좌전하행지 및 우관상동맥에 병소가 위치하는 경우는 인공심폐기의 사용 없이 심장이 박동하는 상태에서 관상동맥우회술을 시행하는 방법도 일부에서는 시행되어 왔으며 최근에는 최소한의 절개하에서 관상동맥우회술을 시행하는 방법도 대두되었다. 본 병원에서는 1996년 3월부터 8월까지 시행한 35례의 관상동맥우회술중 6례에서 인공심폐기를 사용하지 않고 관상동맥우회술을 시행하였다. 환자는 남자 4례와 여자 2례였으며 나이는 55세에서 76세로 평균 64세였다. 수술전 관상동맥의 병소는 6례 모두 좌전하행지에 90%이상의 협착을 보였으며 1례는 대각지가 50%, 또 다른 1례에서는 좌주관상동맥이 50%정도의 협착을 동시에 보였다. 수술에 필요한 개흉방법은 통상적인 정중흉골절개가 1례, 부분흉골절개술을 시행한 경우가 2례, 좌측전흉부를 개흉한 경우가 3례였다. 관상동맥우회술은 5례에서 좌측내흉동맥을 좌전하행지에 문합하였으며, 1례에서는 좌측내흉동맥에 요골동맥편을 단단문합한후 좌전하행지에 문합하였다. 또한 1례는 복재정맥을 이용하여 대각지에도 동시에 우회술을 시행하였다. 수술후 기관발관시간은 4시간에서 14시간으로 평균 9시간이었다. 수술에 사용한 혈액의 양은 평균 800 ml였으며 2례에서는 전혀 혈액을 사용하지 않았다. 이들중 5례에서 수술후 추적관상동맥조영술을 술후 7일에서 10일사이에 시행하여 100%의 개통율을 보였다. 결론적으로 좌전하행지나 우관상동맥에 협착이 있는 경우나 상기병소에 경피적풍선확장술이 실패한 경우에 인공심폐기를 사용하지 않고 최소한의 개흉하에 관상동맥우회술을 시행하는 것도 하나의 방법이라 사료되며 앞으로 기술적 발전과 더불어 적응대상군이 증가할 것으로 판단된다.32, 88.67$\pm$10.22 mmHg로 감소하였고 폐동맥압의 평균은 수술전에 11.4$\pm$5.68 mmHg에서 25.94$\pm$11.53, 29.67$\pm$9.31 mmHg로 증가 하였으나 모두 통계적 의의는 없었다(p>0.05). 양측 폐이식수술에서인 폐동정맥문합부위의 파열, 협착, 뒤틀림 등의 수술수기상의 문제점을 예방하면서 우측폐를 먼저 이식하면서, 폐수술시야를 충분히 확보하고, 재관류손상을 방지하는 경우 cystic fibrosis, pulmonary hypertention, emphysema와 같은 심한 호흡부전증 환자의 치료방법으로 적합하리라 사료된다.에서 선택수술 (elective coronary artery bypass graft)에 비하여 특별한 위험 요소의 증가 관상 동맥 우회술을 적용하여 좋은 결과를 얻을 수 있었으며 이들에 대한 장기 추적이 릴요할 된다.착군에 비하여 의미있게 작았는데 이는 아마도수술 당시 협 착 부위의 완전제거가 이루어지지 않은 것이 원인이라고 사료되었다. 본 분석에서는 어린 연령(3개월 이하), 3개월이하에 시행한 쇄골하동맥편 교약성형술이 의미있는 재협착의 위험요소로 밝혀졌다. 결론 적으로 저자등은 본연구를 통하여 대동맥협부지수, 횡대동맥 지수 등이 개개 대동맥교약 환아의 해부학 적, 임상적 특징을 파악하는데 도움이 되는 도구라는 사실을 발견하였고 아울러 교약의 해부학적 특성, 동반 심기 형, 연령, 수술방법 등이 수술사망 및 재협착에 영향한다는 사실을 입증하였다.t was resulted from increase of weight of single cocoon. "Manta"2.5ppm produced 22.2kg of cocoon. It is equal Minimally invasive coronary artery bypass grafting without using cardiopulmonary bypass (CPB) is a recently accepted modality of myocardial revascularization prcedures which is particularly suitable to the patients with lesions in the left anterior descending(LAD) and the right coronary arteries. Of the consecutive 35 patients of coronary artery bypass grafting performed at Sejong General Hospital from March to August 1996, six patients underwent minimally invasive coronary artery bypass grafting without CPB. All had stenotic lesions of the LAD more than 90%. Bypass grafting of the LAD was approached through midline sternotomy in one, through ministernotomy in two, and through limited left anterior thoracotomy in three patients, respectively. The internal mammary arteries were prepared without the use of thoracoscope. The mobilized mammary arteries were connected directly to the LAD in 5 patients, and the anastomosis required interposition of a segment of the radial artery in the remaining one. The diagonal branch was revascularized with the saphenous vein graft at the same time in one patient. No blood transfusion was necessary in 2 patients, and average blood required during surgery was 800ml in 4 patients. All patients were extubated from 4 to 14 hours(mean 9 hours) after operation. Early postoperative coronary angiography in 5 patients between 7 and 10 days after surgery has proved full patency of the grafts. With these limited clinical experiences, the clinical results demonstrated that minimally invasive coronary artery bypass grafting without CPB is an useful procedure especially in patients with isolated lesion in the proximal LAD.

기관지천식 환자에서 일반 건강과 관련된 삶의 질 평가

김철우(Cheol Woo Kim),박중원(Jung Won Park),정혜원(Hye Won Chung),신재인(Jae In Shin),배상운(Sang Woon Bae),조승연(Seung Yun Cho) 대한천식알레르기학회 2002 천식 및 알레르기 Vol.22 No.3

N/A N/A

대장 및 직장암의 임상적 고찰

박실무(Sill Moo Park),유병철(Byung Chul Yoo),이상재(Sang Jae lee),장세경(Se Kyung Chang),정혁(Hyeok Jeong),정우식(Woo Sik Jeong),손창재(Chang Jae Son),이효랑(Hyo Rang Lee),박중원(Chung Won Park) 대한소화기학회 1993 대한소화기학회지 Vol.25 No.6

N/A Recently, colorectal cancer is one of the major public health problems and the annual incidence has been increased in Korea. We reviewed 175 cases of primary colorectal cancer who were diagnosed at Chungang University Hospital during a 10 year period from January 1982 to December 1991. The ratio of male to female was 1.2:1 and the peak incidence occured in the seventh decade (29.3 %) The location of cancer occurence were rectum (28. 6%), sigmoid colon (26.3%), ascending colon (25. 1%), transverse colon (6.9%), cecum (6.3%) and descending colon (5.7%) in the order of frequency. The most frequent symptoms were abdominal pain in the right sided colon lesions, bowel habit change in the left side colon and rectal lesions. The accuracy of preoperative diagnosis among barium enema (95.4%), sigmoidoscopy or colonoscopy (95. 9%) and computerized tomography (89.6%) was similar. Operation was performed in 86.8% and surgical resection of primary cancer was possible in 88.1% of operation cases. The most common histopathologic type was well differentiated adenocarcinorna(42.3%) and the prevalence of synchronous polyp was 15.4%. According to Dukes' classfication, the distribution was as follows; class A: 1.3%, class B: 48.1%, class C: 35.7%, class D: 14.9%

전국 초등학교 교실 내 유해 환경 평가

임영욱(Young-Wook Lim),이청수(Chung-Soo Lee),김호현(Ho-Hyun Kim),양지연(Ji-Yeon Yang),이건우(Geon-Woo Lee),손종렬(Jong Ryeul Sohn),박중원(Jung-Won Park),신동천(Dong-Chun Shin) 한국실내환경학회 2008 한국실내환경학회지 Vol.5 No.1

This study was performed to investigate airborne volatile organic compounds(VOCs), formaldehyde, respiratory particulate for concentration in primary schools. The concentrations of major indoor air pollutants(VOCs ; benzene, toluene, ethylbenzene, xylene, styrene, formaldehyde, PM-10) were observed from November to December 2006. Sampling was undertaken at 81 primary schools. The sampling sites of air pollutants are classroom and hallway. VOCs with distribution of most of general environmental contamination material will be able to confirm that it shows the log-normal distribution which is similar exposure distribution. The exposure quality of VOCs and the place pollution level was indoor> hallway>outdoor, which whole is located in the metropolis and the industrial areas is higher than farm village area. It tried to observe the I/O ratio, it appeared highly from the interior of the material of most. The mean concentrations of formaldehyde, respiratory particulate were 22.07㎍/㎥, 88.06㎍/㎥ respectively. Indoor and outdoor ratios(I/O) of formaldehyde and respiratory particulate were 3.6 and 1.4, respectively. The concentration of respiratory particulate is 27.2% higher than guideline for school hygiene(100㎍/㎥). From the comparison in the construction year, the highest concentration of formaldehyde is showed under one year. However, as time passed by the concentrations of formaldehyde become lower.

유우에서 발생한 수포양 병변에서 bovine papillomavirus 와 parapoxvirus 혼합감염

류영수,강영배,장정호,문운경,최상호,박중원,Lyoo, Young S,Kang, Yung-bai,Chang, Chung-ho,Moon, Oun-gyong,Choi, Sang-ho,Park, Joong-won 대한수의학회 1998 大韓獸醫學會誌 Vol.38 No.2

A mysterious disease with vesicle-like nodules in dairy cattle udder has been drawn attention to the foreign animal disease expert at the National Veterinary Research Institute. Immediately dispatched foreign animal disease expert, pathologist and regional veterinary officials executed quarantine nearby affected farm area to prevent transmission of the pathogen which was possibly contagious vesicular disease agents in domestic animals such as FMD or SVD. Proper samples were collected for the laboratory examination. Vesicle-like lesions were only detected in lactating dairy cattle and no distinct clinical signs have been observed in affected animals. Parapox and papillomavirus particles have been demonstrated on electromicroscopical examination from nodular samples of udder lesions of the dairy cattle. Characteristic papilloma virus particles with 55nm in diameter and parapoxvirus with 150nm in diameter and 350nm long oval shape particles were detected and confirmed by embryonated chicken egg inoculation.