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Sulbactam의 Penicillin G와 Ampicillin 항균력에 대한 상승작용
정윤섭,권오헌,이삼열 대한화학요법학회 1983 대한화학요법학회지 Vol.1 No.2
β-lactam 항생제는 대단히 널 이용되어 왔으나, 이제는 많은 세균이 이들 항생제에 내성이 되었기 때문에 그 유용성이 현저히 감소되었다(김 등, 1983; 이 등, 1983). β-lactam계 항생균에 대한 내성은 흔히 세균이 이 항생제를 분해하는 효소인 β-lactamase를 생산함에 기연된다. 이러한 세균의 감염에는 β-lactamase reaistant antibiotic을 투여하여 왔다. 한편 β-lactamse를 억제하는 물질을 β-lactam 항생제와 동시에 투여함으로써 치료효과를 얻으려는 연구들이 근년에 이루어지고 있다. β-lactamse inhibitor 중에는 sulbactam(CP-45,899, penicillanic acid sulfone). clavulanic acid, 6-beta-iodo penicillanic acid(UK-38,006) 등이 있다(Moosdeen et at., 1981). Sulbactam의 상승 작용은 군종과 군주에 따라 다른 것으로 알려져 있으므로(Fass 1981) 이 연구에서는 Penicillin G와 ampicillin의 항균작용에 대한 sulbactam의 상승작용에 관하여 임상검체에서의 분리세균을 써서 실험하였다. S3nergistic effect of sulbactam is known t o vary depending not only on the species of bacteria but also on the isolates of the same species. The effect was eva1uated;using bacterial strains isolated from Yonsei Medical Center patients, b5- the agar dilution method. Synergistic effect of sulbactam on the activity of penicillin G and ampic in all species of bacteria tested. The effect was slight in S. aweus and S. marcescens, =oderate ir, hi. gonorrhoeae, E. coli, K. pneumoniae, Protezrs and H. influenzae, and significant in S. f l e z n e r i and A. calcoaceticus. It is concluded that sulbactan: can broaden the antibacterial spectrum of. penicillin G and ampicillin.
Haemophilus influenzae의 Ampicillin과 Cefaclor 감수성
정윤섭,이삼열 대한화학요법학회 1985 대한화학요법학회지 Vol.3 No.2
Haemophilus influenzae cause various infections including such sarious ones as meningitis. Ampicillin is the drug once used widely to treat haemophilus infections, but current isolates often shows resistance to this drug. Cefaclor is an-oral cephlosporin reported to be active against both β-lactamase positive and negative isolates of H. influenzae. In this study susceptibility of H. influenzae isolated from clnical materials at Yonsei Medical Center during 1983~1985 were tested 14% were resistant to ampicillin and 9% were β-lacatamase producers. To cotrimoxazole 26% of the strains were resistant, but less than 0.5% to 1.5% of the isolates were resistant to cephalothin, cefamandole, cefaclor, chloramphenicol, erythromycin and tetracycline. Minimum inhibitory concentration of cefaclor was ≤8 ㎍/ml against both β-lactamase-positive and negative isolates. Cefaclor may be a useful drug for the treatment of H. influenzae infection with the exception of meningitis.
염기성 세균 배양을 위한 Steel Wool법과 Gaspak법의 비교
정윤섭 한국미생물학회 1974 미생물학회지 Vol.12 No.3
It is a well-known fact that an isolation of non-sporeforming anaerobes, considered normal flora in man ordinarily but causes serious infections sometimes, is a dificult procedure because of their great oxygen sensitivity. Among the many techniques employed in clinical laboratories, despite of its high expenses, the GasPak method has been most widely used because of its relative simplicity. On the other hand, the steel wool method has gained a good reputation recently. This technique makes it possible to treat individual plate so that any single specimen can be promptly cultured anaerobically. The procedure is quite simple and the expenses are negligible. In the present study it is to compare these two methods as to their efficiency of anaerobic cultivation using 13 VPI strains of non-sporeforming amaerobic bacteria. Among the 13 species the following 11, Bacteroides fragilis ss. fragilis, B. fragilis ss. thetaiotaomicron, propionibacterium acnes, Eubacterium limosum, E. lentum, peptococcus asaccharolyticus, Pc. prevotii, Pc. magnus, peptostreptococcus anaerobius, Ps. intermedius nad Veillonella parvula, grew well with the steel wool method whose colony numbers reaching 57 to 119% of those with GasPak method. The remaining two species, Fusobacterium nucleatum and F.necrophorum, did not grow well with the steel wool method showing the colony numbers were only 0.4% of those with GasPak method in the case of Fusobacterium nucleatum. In the case of Fusobacterium necrophorum, very few colonies developed even with a heavy inoculation. As to the size of colonies, there were no significant difference between these two methods.
Ampicillin과 Chloramphenicol 내성 Salmonella typhimurium 분리의 증가
정윤섭,한상순,권오헌,이삼열,정태화,Chong, Yun-Sop,Han, Sang-Soon,Kwon, Oh-Hun,Lee, Samuel.Y.,Jung, Tae-Hwoa 대한미생물학회 1987 大韓微生物學會誌 Vol.22 No.1
Salmonella typhi infection, which was the most frequent enteric infection in Korea, has been decreasing, while the infection of other serogroups of Salmonella has been increasing since the later part of 1970s. In 1986, the number of serogroup B isolated by us increased to 46, which corresponds 21.1% of all enteropathogenic bacteria isolated from stool specimens. Salmonella isolates resistant to antimicrobial agents were extremly rare in Korea, in the 1970s. However, 7 of 13 serogroup B isolates showed resistance to ampicillin or to chloramphenicol in 1984. Among the serogroup B isolates in 1986, 71.7% and 69.6% were resistant to ampicillin and to chloramphenicol respectively. The minimum inhibitory concentrations of ampicillin and chloramphenicol against these isolates were >$128\;{\mu}g/ml$ and $128\;{\mu}g/ml$ respectively.