심내막염 환자의 동종이식편 치환술 -1례 보고-

박중원,박국양,김웅한,Park, Jung-Won,Park, Guk-Yang,Kim, Ung-Han 대한흉부심장혈관외과학회 1997 Journal of Chest Surgery (J Chest Surg) Vol.30 No.8

Prosthetic valve endocarditis(PVE), although uncommon, is associated with significant mortality if the infection spreads into the paravavular structures with later abscess formation. However, combined antibiotic and surgical treatment is often successful. Accurate diagnosis by on echocardiography, effective myocardial protection during operation and increased surgical experience have improved the short-term and long-term outcomes for patients with PVE. A 35-year-old male had a history of replacement of aortic and mitral valve, and tricuspid annuloplasty on August 1994, was admitted due to sudden onset of aphasia, leftward deviation of both eyeballs and spiking fever and diagnosed of having PVE by echocardiography. Reoperation was done after 6weeks of antibiotic treatment. On the operative field, we could notice circumferential vegetation along aortic valve annulus, paravalvular leakage and abscess pocket. The mitral valve amlulus was healthy. The patient underwent redo aortic valve replacement using cryopreserved aortic homograft after radical debridement of infected issue. During the follow up of 7 months period the homograft was well functioning without recurrence of symptoms. 인공판막심내막염은 드물지만 합병증으로 판막주위가 감염으로 파괴되고 농양이 형성되는 경우가 있어 높 은 사망율을 가져온다. 그러나 적절한 항생제의 사용, 심장초음파 검사에 기초를 둔 조기 진단,수술시 효과적인 심근보호. 그리고 수술경험의 축적으로 인공판막심내막염에 있어서 보다좋은 장단기 결과를 얻을 수 있다. 35세 남자 환자가 94년 8월에 대동맥판막, 승모판막대치술과 함께 삼첨판륜성형술을 받은후 특별한 증상 없이 지내다가 갑자기 시작된 실어증, 양안구의 좌측편위, 고열로 입원하여 심장초음파 검사를 시행한 결과 인공판막 심내막염 소견을 보여 6주간의 항생제 치료후 수술을 시행하였다. 수술소견상 대동맥 판륜을 따라 증식물 소견과 판막 주위의 누출, 농양주머니가 형성되어 있었고 승모판막은 비교적 건강해 보였다. 수술은 감염된 조직의 전체적인 제거와 냉동저장된 대동맥 동종이식편을 이용하여 재대치 수술을 시행했다. 환자는 수술후 63일째 퇴원했으며 퇴원후 7개월 동안 외래 추적 관찰결과 특별한 이상소견 없이 지내고 있다.

발생중인 생쥐 치아 및 치주조직에서 치주인대-특이 단백질; PDLs22의 발현

박중원,박병기,김상목,김병옥,박주철,Park, Jung-Won,Park, Byung-Ki,Kim, Sang-Mok,Kim, Byung-Ock,Park, Joo-Cheol 대한치주과학회 2002 Journal of Periodontal & Implant Science Vol.32 No.1

The periodontal ligament(PDL) is a unique tissue that is crucial for tooth function. However, little is known of the molecular mechanisms controlling PDL function. PDL-specific protein;PDLs22 had been previously identified as a novel protein isolated from cultured human PDL fibroblasts using subtraction hybridization between human gingival fibroblasts and PDL fibroblasts. The aim of this study was to examine the expression pattern and tissue localization of PDLs22 protein in embryonic and various postnatal stages of developing mouse using immunohistochemical staining. Embryos (E18) and postnatal (P1, P4, P5, P15, P18) were decapitated and the heads were fixed overnight in a freshly prepared solution of 4% paraformaldehyde. Some specimens were decalcified for $2{\sim}4$ weeks in a solution containing 10% of the disodium salt of ethylenediamine-tetraacetic acid (EDTA). Next, tissues were dehydrated, embedded in paraffin and sectioned serially at $6{\mu}m$ in thickness. Polyclonal antiserum raised against PDLs22 peptides, ISNKYLVKRQSRD, were made. The localization of PDLs22 in tissues was detected by polyclonal antibody against PDLs22 by means of immunohistochemical staining. The results were as follows; 1. Expression of PDLs22 protein was not detected in the tooth germ of bud and cap stage. 2. At the late bell stage and root formation stage, strong expression of PDLs22 protein was observed in developing tooth follicle, osteoblast-like cells, and subodontoblastic cells in the tooth pulp, but not in gingival fibroblasts, ameloblasts and odontoblasts of tooth germ 3. In erupted tooth, PDLs22 protein was intensely expressed in PDL and osteoblast-like cells of alveolar bone, but not in gingival fibroblasts, mature osteocytes and adjacent salivary glands. 4. In the developing alveolar bone and mid-palatal suture, expression of PDLs22 protein was seen in undifferentiated mesenchymal cells and osteoblast-like cells of developing mid-palatal suture, but not in mature osteocytes and chondrocytes. These results suggest that PDLs22 protein may play an important role in the differentiation of undifferentiated mesenchymal cells in the bone marrow and PDL cells, which can differentiate into multiple cell types including osteoblasts, cementoblasts, and PDL fibroblasts. However, more researches should be performed to gain a better understanding of the exact function of PDLs22 protein which related to the PDL cell differentiation.

새로운 국내 분리 토끼출혈병바이러스(RHDVa)를 감염시킨 토끼에서의 경시적인 병리학적 변화와 조직 내 바이러스 항원 분포

박중원,전지은,양동군,박은정,김한,이명헌,황의경,이중복,우계형,Park, Jung-Won,Chun, Ji-Eun,Yang, Dong-Kun,Bak, Eun-Jung,Kim, Han,Lee, Myeong-Heon,Hwang, Eui-Kyung,Lee, Chung-Bok,Woo, Gye-Hyeong 대한수의학회 2012 大韓獸醫學會誌 Vol.52 No.2

Rabbit hemorrhagic disease is a highly acute and fatal viral disease caused by rabbit hemorrhagic disease virus (RHDV). Since first outbreak in Korea 1987, RHDV has been continually affected in the country, but the pattern of outbreak seem to be changed. In this study, to understand the pathogenesis of the new RHDVa serotype, we therefore carried out to inoculate RHDVa to rabbits, and to examine the sequential histopathologic changes and viral distribution. Macroscopically, various sized dark red or white spots or appearance were observed in the liver, lung, kidney uterus and ureter. In euhanized rabbits, significant pathologic findings such as infiltration of heterophils and mononuclear cells were observed at 24 hours after inoculation (HAI), and these were sequentially extended periportal to centrilobular area. However, in dead rabbits, severe hepatic degeneration and/or necrosis with relatively weak inflammatory responses were observed. RHDV antigens began to detect in liver, spleen, and lung from 12 HAI by PCR. Immunohistochemically, RHDV positive cells were seen in only liver from 24 HAI, and the degree of immunogen reactivity was stronger in dead rabbits than in euthanized ones. In conclusion, RHDVa caused the subacute or chronic infection accompanying low mortality and moderate to severe inflammatory reaction in rabbits, suggesting the possibility that RHD could become endemic.

국내 분리 토끼출혈병 바이러스(RHDVa)를 감염시킨 토끼 간장에서의 경시적인 초미세구조 변화와 apoptosis

박중원,전지은,박은정,김한,이명헌,황의경,김재훈,이중복,우계형,Park, Jung-Won,Chun, Ji-Eun,Bak, Eun-Jung,Kim, Han,Lee, Myeong-Heon,Hwang, Eui-Kyung,Kim, Jae-Hoon,Lee, Chung-Bok,Woo, Gye-Hyeong 대한수의학회 2013 大韓獸醫學會誌 Vol.53 No.1

In this study, to understand the pathogenesis of new rabbit hemorrhagic disease virus (RHDVa) serotype, we carried out to administrate RHDVa to rabbits, and to examine sequential electron microscopic changes and relationship between pathogenesis and apoptosis. TUNEL-positive cells began to be observed from 24 hours after inoculation (HAI) and the number of positive cells was slightly increased with the course of time. Whereas marked increase of positive cells was seen in the liver from the rabbits died acutely. Typical viral particles with cup-like projections and a diameter of 30~40 nm were detected in homogenized liver samples and tissues at 36 and 48, and 48 HAI, respectively. Ultrastructurally, glycogen deposition was observed from the first stage of hepatocellular degeneration by RHDVa infection and then, swelling and disruption of cristae of mitochondria by viral particles, swelling of smooth endoplasmic reticulum, vacuoles and vesicles were detected. Condensation, margination and fragmentation of chromatin were observed in degenerative hepatocytes at 36 and 48 HAI, indicating apoptotic bodies. These data offer that hepatocytic apoptosis by RHDV infection could be closely related with mitochondrial impairment in the hepatocytes.

디자인·공예 융합 교육 프로그램 사례연구 -국민대학교 「팀팀Class」를 중심으로-

박중원,Park, Jung-won 한국디지털정책학회 2021 디지털융복합연구 Vol.19 No.8

융합이라는 시대의 흐름과 더불어 상상력과 창조성에 기반을 둔 디자인·공예의 본질적 속성의 효과적 결합을 위해서는 학문적, 방법적 측면에서 다양한 시도가 필요하다. 본 연구는 2020년 2학기에 실행된 국민대학교 팀팀Class의 디자인·공예 융합 교육 프로그램의 운영 사례를 분석하는 것이다. 이를 위해 기존 문헌연구를 통한 디자인·공예 융합 교육 관련 중요 항목을 5가지(대상, 방법, 운영, 공간, 소통)로 구분해 분석하였다. 이를 통해 디자인·공예 융합 교육 프로그램에 대한 경험과 문제점, 그리고 향후 디자인·공예 융합 교육 프로그램의 운영에 적용 가능한 해결방안 제시에 본 연구의 목적이 있다. 본 연구에서는 국민대학교 팀팀Class 디자인·공예 융합 교육 프로그램으로 사례연구 대상을 한정하였으나 향후 지속적인 연구를 통해 향후 다가올 코로나(COVID-19) 이후 시대의 새로운 환경에 적용 가능한 디자인·공예 융합 교육 프로그램을 위해 후속 연구가 진행되어야 할 것이다. The tendency of the current times require education to focus on convergence, and the same applies to the essence of ceramics and design base imagination and creativity. For effective integration, a wide range of experimentations is required both in terms of academic and methodic approaches. This study analyzes the [TeamTeam Class] curriculum, converging ceramics with design initiated in the second semester (autumn semester) of 2020. Through reference materials on ceramics and design convergence education, it was possible to classify the following 5 categories: Subject, Method, Management, space and communication. The aim of the study is to find resolutions to overcome existing issues and problems in search of more effective methods. Although this study is based on convergence education, [TeamTeam Class] at Kookmin University, I hope to extend it further to also consider education after COVID-19.

하이브리드의 방법적 접근

박중원(Park Jung Won) 한국조형디자인학회 2016 조형디자인연구 Vol.19 No.3

많은 것들이 합쳐지고, 서로의 영역을 넘나들면서 다양함과 새로움을 만들어 내는 시대적 현상은 하이브리드와 같은 사상의 발전에 밑바탕이 되었다고 볼 수 있다. 이렇듯 하이브리드 현상은 이제 우리 시대의 분위기를 대표하고, 함축하는 것 이상의 문화현상이 되었다. 즉, 두 가지 이상의 요소나 특징들이 조합되어 또 다른 형태나 양식을 만들어내는 하이브리드 현상은 현 시대 사회, 문화, 예술에 나타나고 있는 일반적인 특징이다. 또한, 현시대의 많은 부분에서 목격되는 하이브리드는 미래사회의 진보를 예견하는 척도가 될 것이다. 그러나 하이브리드에 대한 여러 관심에도 불구하고, 도예에서의 하이브리드에 대한 비전을 구체적으로 제시해 줄 수 있는 학문적 연구가 부족하며, 이것은 현대도예에서 하이브리드에 대한 진지한 담론이 필요한 이유이다. 본 연구는 하이브리드와 도예의 접목으로 인한 2, 3차원적 표현이 21세기에 들어 급격하게 진화중이라는 점에 주목한다. Ⅱ장에서는 하이브리드의 일반적 정의 및 단계적으로 구조화된 결합논리를 살펴보고, 제롤드 레빈슨(Jerrold Levinson)이 제시한 하이브리드의 결합 형식을 소개한다. Ⅲ장에서는 도예에서 하이브리드의 영향과 의미를 살펴보고, 하이브리드 도예의 사례를 유형별로 분석한다. 또한 하이브리드 도예의 특성과 미래전망을 알아보고, 하이브리드의 현대도예에서의 의미와 역할 그리고 창조의 비전을 발견하고자 한다. 이를 통해 도예에서의 하이브리드 적용과 영역확장에 있어서 도예가들이 가져야할 문제의식과 창조적 태도를 제시하고자 한다. The periodic phenomenon that generates diversity and newness, through crossovers of may different realms, namely with each other and through their amalgamation, provides the background for developing concepts associated with fusion, and convergences of hybrid and consilience. This phenomenon which creates different forms and manners by combining two or more things together, is an overall hallmark in society, culture, and art of present day. Furthermore it is observed frequently in the contemporary period because it is one measure to predict change, progress and potential for a future society. This is precisely why a discourse on the hybrid concept in contemporary ceramics is crucial. This research analyses the conceptual principle and functional property of hybrid, and proposes methods of hybrid and development processes, which are adaptable to practice-based research in contemporary ceramic art. The central axis in terms of approach is based on the concept of hybrid art forms as exemplified in the investigation of Jerrold Levinson as explained in the second part of the research. The use of hybrid forms provides a moment to explore the underlying nature on different things, and this investigation presents a knowledgeable base to appreciate, compartmentalize, and integrate the differences between them. The methodological approach of hybrid for integrated creation supplements conceptual discourses and methodological transformations in the field of ceramic art, and with it is the expectation of a paved way for further investigations and developments by other practitioners. This is explained in the third part of the research. The importance of applying the concept of hybrid in contemporary ceramics is emphasized in the research. This is crucial for the expansion of the field and also for future developments.

경증 기관지 천식 환자에서 Methacholine 흡입에 따른 Spirometer와 Body Plethysmographic Parameter의 변화에 관한 연구

박중원 ( Park Jung Won ),홍천수 ( Hong Cheon Su ) 대한내과학회 1993 대한내과학회지 Vol.44 No.2

연구배경 : Methacholine 유발검사시 일반적으로 FEV_(1)이 기저값에 비해 20% 이상 감소하면 양성으로 판정한다. 그러나 spirometer 변수는 기도저항뿐만아니라 환자의 근육상태 그리고 환자의 의도 등에의해서 많은 영향을 받는다. 또한 자연 증상 발현성 기관지 천식환자에서의 기관지 수축과 methacholine 유발 기관지 수축간에 병태 생리학적으로 차이가 있을 것으로 생각된다. 방법 : Methacholine 유발검사의 객관적인 양성 반응의 판정을 위해서 methacholine 유발검사 전 과 후에 각각 spirometer와 plethysmography를 측정하였다. Methacholine에 의한 기관지 수축의 기전을 이해하기 위해서 methacholine 유발 기관지 수축군과 중등도의 자연 증상 발현성 기관지 천식군 각각 18예를 대상으로 spiromter, plethysmography, 그리고 CO폐 확산능을 비교하였다. 결과 : 경증의 기관지 천식환자에서 methacholine 유발검사 후에 시행한 plethysmography 소견상 30예전 예에서 Raw가 175% 그리고 SGaw는 239% 증가하였으며 30예전 예에서 Raw가 35%이상 증가하였다. RV은 methacholine 유발검사 후 평균 33.5%가 증가하였다. 그러나 30예중 7예는 methacholine 유발검사 후 RV가 기저값에 비해 감소하였다. VC와 DLCO는 methacholine 유발검사 후 기저값에 비해 각각 15.1%, 8.7%씩 의미있게 감소하였으나 ERV 및 TLC는 methacholine 유발검사 전과 후에 통계학적으로 유의한 차이를 발견할 수 없었다. Methcholine 유발검사 후의 FEV1 감소분과 RV 및 Raw의 증가분 간에는 유의한 상관관계를 찾을 수 없었다. Methacholine 유발검사 후의 FEV_(t)의 변화와 Vmax50, Vmax25의 변화간의 상관관계를 조사한 바 의미있는 상관관계를 관찰할 수 없었으나 methacholine 유발검사 후 30예 전 예에서 Vmax50이 기저값에 비해 20% 이상 감소하였고 Vmax25는 27예에서 20%이상 감소하여 methacholine 천식유발검사에서 객관적인 양성판정 기준으로 Vmax50, Vmax25등이 유용함을 알 수 있었다. Methacholine에 의한 기관지 수축군과 중등도의 자연 증상 발현성 기관지 천식군간의 spirometer, plethysmography 그리고 DLCO를 비교한 바 spirometerqus수와 DLCO는 두 군간에 유의한 차이가 관찰되지 않았다. 그러나 body plethysmography 변수에서는 methacholine 유발 기관지 수축군이 380.9±243.9% 대 247.8±102.0%로 자연 증상 발현성 기관지 천식군에 비하여 SGaw가 유의하게 높았으며 그이외의 다른 plethysmogrpahy 변수에서는 두군사이에 유의한 차이가 관찰되지 않았다. 결론 : 이상의 결과로 객관적으로 비특이적 기관지 과민도를 판정하는데 Vmax50, Vmax25 그리고 Raw가 유용함을 알 수 있었으며 비록 병태 생리학적으로 자연증상 발현성 기관지 천식과 methacholine에 의한 기관지 수축사이에 차이가 있어도 methacholine 유발 기관지 수축 상태의 spirometer 및 plethysmography 소견은 자연 발현 기관지 천식의 폐기능을 반영하며 기관지 천식의 폐기능 연구에 methacholine 유발검사가 유용함을 알 수 있다. Background : Methacholine inhalation test was known to be a good method for assessing the nonspecific bronchial hyperresponsiveness(NSBH). And a decline in forced expiratory volume in 1 second(FEV1) of more than 20% indicates the presence of NSBH. But there are some difficulties in determination of NSBH objectively as the results of spirometry are influenced by ptients motivations. And there are same differences in the mechanisms of airway narrowing between natural bronchial asthma and methacholine induced bronchial spasm. Methods : We measured spirometric and body plethys-mographic parameters both before and after inhalation of methacholine in 30 cases of mild asthmatics. To better understand the mechanism of the methacholine induced bronchospam, spirmetric and plethysmogra-phic parameters of methacholine induced bronchospasm(MIBS, n=18) were compared with natural bronchial asthma(n=18). The MIBS and natural bronchial asthma groups were matched with same predict value of FEV1(65 vs 67%)Results : After the inhalation of methacholine, maximal expiratory flow volume at 50% of total lung capacity(Vmax50) was decreased more than 20% compared to baseline value in all 30 cases and the Vmax25 was decreased more than 20% in 27 of the 30 cases. Inhalation of methaholine produced an 175% increase increase in airway resistance(Raw)and 239% increase in specific airway resistance(SRaw), and all of the 30 cases had an increase in Raw of more than 35 percent. Methacholine inhalation produced a 33.5% increase in residual volume (RV), however the RV was decreased or remained unchange after inhalation of methacholine in 7 out of 30 cases. There were no differences in total lung capacity and effective residual volume between before and after inhalation of methacholine. Vital capacity and diffusing capacity of Co after challenge were significantly decreased 15.1% and 8.7% respectively. With comparison of MIBS(n=18) and natural bronchial asthma(n=18), SRaw was significantly higher in MIBS than natural bronchial asthma (380.±243.7 va 247.8±102.0% of pred.) and no significant difference was found in all spirometric and other plethysmographic parameters. Conclusion : For assessing the NSBH, Raw, Vmax50 and Vmax25 are useful and objective parameters. And even though there are some pathophysologic differences between MIBS and natural bronchial asthma, we could not find significant differences in most parmeters of spirometer and plethysmographic parameters except SRaw between MIBS and natural asthma.

대한간학회지 1999년 추계학술대회 초록집 : 간에서의 아포토시스: 현재와 전망

박중원 ( Park Jung Won ) 대한간학회 1999 Clinical and Molecular Hepatology(대한간학회지) Vol.5 No.3(S)

대한내과학회가 당면한 과제

박중원 ( Jung-won Park ) 대한내과학회 2022 대한내과학회지 Vol.97 No.6

Since its establishment in 1945, the Korean Association of Internal Medicine (KAIM) has had a proud history of overcoming challenges with the combined and harmonized endeavors of all KAIM members. KAIM is now facing several issues related to marked social changes in Korea as well as revolutions in medical science and scientific and communications technologies. Furthermore, KAIM shortened the resident training program in 2017. Internal medicine (IM) is an essential discipline for maintaining and improving the health of Korean citizens. However, the Korean Ministry of Health and Welfare lacks initiatives for supporting IM, and the Korean Health Insurance reimbursement policy does not reflect the essential role of IM in health care. This is aggravated by imbalances in hospital resources and skewed notions in patients’ preferences with regard to metropolitan and non-metropolitan areas. Limited preferences in application to subspecialties may be an obstacle for coordinated development of IM. The current generations of students and residents are familiar with online educational lectures and have different needs for educational materials, which are not met by conventional educational materials. Recent experiences in the COVID-19 pandemic have exacerbated or accelerated these changes and problems. From this perspective, I will summarize the challenges that KAIM is now facing, and outline some possible solutions. (Korean J Med 2022;97:335-338)

제 26 회 대한 천식 및 알레르기학회 추계학술대회 : " 한국의 성인 기관지천식환자의 삶의 질 평가를 위한 설문 - KAQLQ " 의 개발 및 유용성 평가를 위한 다기관 연구

박중원(Jung Won Park),조유숙(You Sook Cho),이순영,남동호(Dong Ho Nahm),김윤근(Yoon Keun Kim),문희범(Hee Bom Moon) 대한천식 및 알레르기학회 ( 구 대한알레르기학회 ) 1999 추계학술대회 초록집 Vol.1999 No.-