암-특이 항원인 alpha-Fetoprotein 에 대한 모노클론항체의 생산 및 분석

박대규,방옥선,강신성,조동택,송방호 경북대학교 유전공학연구소 1987 遺傳工學硏究所報 Vol.2 No.1

Monoclones secreting anti-human alpha-fetoprotein(AFP) were produced by hybridizing SP 2/0-Ag 14 mouse myeloma cells with spleen cells of Balb/c mice immunized with AFP. Two subclones (D-6 & E-6) were expanded as ascite tumors in syngenic mice, and immunoglobulins were purified from ascitic fluid. Each antibody was identified to be homogeneous monoclonal antibody (McAb) by several criteria, and the affinity constant of D-6 and E-6 McAb to AFP was calculated to be 4.2×10^(-8) and 6.4×10^(-8) M^(-1), respectively. With these McAbs sensitive and accurate enzyme linked immunosorbent assay method was established.

SOL-GEL법을 이용한 Ta₂O_(5) 감지 게이트 pH-ISFET의 제조와 그 동작특성

장기석,이정철,조병욱,권대혁,김창수,손병기 경북대학교 센서기술연구소 1995 센서技術學術大會論文集 Vol.6 No.1

Low drift pH-ISFET is presented in this paper. Ta_(2)O_(5), known as a good pH sensing membrane, was formed by sol-gel method on Si_(3)N_(4)/SiO_(2) gate of pH-ISFET. It has been reported that the drift of the pH-ISFET is caused by the surface deterioration or hydration of sensing membrane. Hydration is resulted from diffusion of ions into the sensing membrane. It is reported that Ta205 film does not show surface deterioration but hydration while Si_(3)N_(4) film is not hydrated but its surface is deteriorated. Therefore, ultra thin(about 100Å) Ta_(2)O_(5) sensing membrane was formed by sol-gel method on the Si3N4to shorten the hydration time. The fabricated Ta205 pH-ISFET showed good sensitivity(58~59 mV/pH) in a wide pH range(pH3~11) as well as very low drift (0.06 pH/day)

급성 골수성 백혈병(M6)에 합병된 중추성 요붕증 1 예

김성연,조보연,이홍규,고창순,민헌기,김병국,김노경,신찬수,이명식,강덕현,조종태 대한내과학회 1989 대한내과학회지 Vol.37 No.2

Leukemia is known to be an extremely rare cause of diabetes insipidus. We report a case of diabetes insipidus as a complication of leukemia. An 18 year-old man was admitted because of polyuria polydipsia and fever. Bone marrow aspiration and biopsy revealed the diagnosis of acute erythroleukemia (AML, M6). On the water deprivation test, the diabetes insipidus was confirmed to be of the central type. He was managed with intranasal vasopressin for diabetes insipidus with good response. Induction chemotherapy was administered without response, and the patient died of septicemia and respiratory failure 2 months after initial diagnosis. A short review of the literature related to this subject is also presented.

사료의 pH 및 단백질 수준이 자돈의 성장 및 영양소 소화율에 미치는 영향

김지훈,한인규,김진동,조원탁,현충남,이지훈 한국영양사료학회 1999 韓國營養飼料學會誌 Vol.23 No.4

자돈사료내 pH와 단백질 수준의 효과를 알아보기 위하여 2개의 시험을 수행하였다. 시험 1에서는 pH 수준이 자돈의 성장과 영양소 소화율에 미치는 영향을 총 150두의 자돈(L×D×LW ; 8.82㎏ BW 30 ± 2일령)을 이용하여 1) pH 4.7, 2) pH 5.2, 3) pH 5.7 (basal diet), 4) pH 6.2, 5) pH 6.7의 다섯처리를 두었고, 시험 2에서는 pH와 단백질 수준의 효과를 알아보기 위하여 150두의 자돈을 (L×D×LW ; 5.46㎏ BW) 2×3 요인법으로 배치하였다. (조단백질 = 21.5, 24.5%, 사료 pH = 4.5, 5.5, 6.5). 또한 소화율의 측정을 위하여 시험 1에는 25두, 시험 2에는 24두의 자돈에 T-cannular를 설치하였다. 시험 1에서 pH는 자돈의 성장에 아무런 영향이 없는 것으로 나타났다. 낮은 pH사료를 급이한 구에서 사료효율이 약간 좋은 경향이 있었으나, 유의적인 차이는 발견되지 않았다. 회장 및 분소화율도 처리에 의한 효과가 발견되지 않았다. 시험 2에서는 낮은 pH 사료를 급이한 구에서 성장 및 사료효율이 개선되는 경향이 있었다 (P = 0.16). 사료효율은 단백질 수준에 의한 영향이 있었으며 (P<0.05), pH와 단백질 수준간의 상호작용이나 다른 효과는 발견되지 않았다. 회장 소화율도 단백질 수준에 의한 영향은 있었으나, pH의 효과는 methionine의 소화율에만 영향이 있는 것으로 나타났다. 전체적으로 소화율은 pH에 의한 일정한 경향이 발견되지 않았다. 본 시험의 결과는 사료의 pH 조절효과는 자돈의 나이나 사료의 종류에 따라 영향을 받는다는 것을 암시하며, 일반적으로 통용되고 있는 자돈사료는 특별한 산성화 처리가 필요하지 않은 것으로 나타났다. Two experiments were conducted to investigate the effect of diet pH and protein levels on the growth performance and nutrients digestibility in young piglets. In Exp. 1, a total of 150 piglets (Landrace × Duroc × Large White ; 8.82㎏ B W, 30 ± 2 days of age) were allotted into 5 treatments. Treatments were 1) pH 4.7, 2) pH 5.2, 3) pH 5.7 (basal diet), 4) pH 6.2 and 5) pH 6.7. In Exp. 2, the effect of dietary protein and diet pH levels on the growth performance and nutrient digestibility was examined with 150 crossbreds Landrace × Duroc × Large White ; 5.46㎏ BW) piglets in 2 × 3 factorial arrangement. (21.5 and 24.5% CP and diet pH (4.5, 5.5 and 6.5)). Each treatment has 5 replications, 5 heads per replicate. For the digestibility determination, 25 pigs for Exp 1. and 24 pigs for Exp. 2. were fitted with simple T-cannula and individually fed the experimental diet. In Exp. 1, no significant difference was found in any of the criteria measured. Pigs fed low pH diets showed slightly better feed efficiency, but the difference was not significant. Both ileal and fecal nutrient digestibility were not different. A small difference was found in major amino acids digestibility, but there was no constant trend detected. In Exp. 2, pigs fed low pH diet showed a trend (P = 0.16) to grow faster than those fed high pH diet. Feed efficiency was affected by protein level (P<0.05). No other main effect was detected. Protein level affected the ileal digestibility of energy, dry matter, crude fat, methionine and threonine. The effect of pH was found only in methionine digestibility. No other main effect was found in ileal digestibility. It appeared that age of pigs and type of diet could greatly influence the effect of diet pH manipulation. Acidification of commercially available diets might have minimal effect when diet contained high portion of high quality feed ingredients.

급성췌장염에 의한 췌장가성남종에서 내시경적 배액술후 발생한 췌장가성남종과 십이지장 및 대장에 발생한 내부 췌장루 1예

김연수,이문성,김진오,조영덕,이영홍,봉형근,조주영,이준성,심찬섭,황성규,전기원,변재호,장재영 대한소화기학회 1996 대한소화기학회지 Vol.28 No.1

성인에서 발견된 십이지장 막양구조

박경규,박형근,김연수,이문성,김진오,조영덕,이영홍,봉형근,조주영,이준성,심찬섭,황성규,함정식,장재영,권귀향 대한내과학회 1997 대한내과학회지 Vol.53 No.2

Duodenal obstruction is the third leading cause of intrinsic obstruction of the alimentary tract in the newborn, the other causes are imperforate anus and esophageal atresia. If the obstruction is incomplete, as with a fenestrated duodenal membrane, the presentation of the symptoms may be delayed and the diagnosis overlooked. And so they are rarely diagnosed in the adult. Diagnosis can be made by contrast duodenography but is more definitive with endoscopy. This is the first case report of a 21-year-old female with duodenal membrane, associated with incomplete rotation of intestine and uterus bicornis, diagnosed by hypotonic duodenography and endoscopy, preoperatively, and successfully treated with duodenoduodenostomy.

14 일령 이유자돈을 위한 적정 LACTOSE : SUCROSE 비율 SUCROSE RATIO FOR THE PIGS WEANED AT 14 DAYS OF AGE

한인규,김진동,조원탁,한영근,이찬호,이기웅,박향숙 한국영양사료학회 1999 韓國營養飼料學會誌 Vol.23 No.6

본 실험은 lactose에 대한 sucrose 대체가 14일령 조기 이유자돈의 성장에 미치는 영향을 알아보기 위하여 수행하였다. lactose와 sucrose의 적정비율을 결정하기 위해 평균체중 5.02±0.18 ㎏인 14일령 이유자돈 80두를 4처리 4반복, 반복당 5두씩 완전임의로 배치 하였으며, 처리구별 lactose와 sucrose의 비율은 각각 100:0(처리구 A), 75:25 (처리구 B), 50:50 (처리구 C), 25:75 (처리구 D) 및 0:100 (처리구 E)이었다. 이유초기 7일동안 뿐 아니라 시험 전 기간에 걸친 자돈의 일당중체량 및 일당사료섭취량은 처리 평균간 통계적인 유의차를 나타내지 않았으며, 다만 시험 전기간에 걸친 사료요구율에 있어서만 유의차가 인정되었으며, B와 D 처리구가 특히 우수한 것으로 나타났다 (p<0.05). 이유 1주동안 (Phase I)의 영양소 소화율은 DM과 CP를 제외하고는 처리 평균간 유의차가 인정되지 않았으며, A 처리구의 CP소화율은 다른 처리구에 비해 유의적으로 높았다 (P<0.05).그러나 B, C, D 그리고 E 처리구간에는 유의적인 차이가 보이지 않았다. 이유 14∼21일령 (Phase II)의 영양소 소화율은 처리평균간 유의차가 인정되지 않았으며, 이유 3주간에 걸친 건물, 질소 및 인의 배출량 역시 처리 평균간 유의차를 나타내지 않았다. 본 실험결과에서 나타난 바와 같이 14일령 이유자돈에 대한 탄수화물 공급원으로서의 lactose에 대한 sucrose의 대체는 자돈의 생산성 감소 및 영양소의 배출량 증가와 같은 불리함이 없이 이루어질 수 있음을 의미한다. This experiment was conducted to determine the optimum ratio of lactose to sucrose for nursery pigs weaned at 14 days of age. A total of 80 weaned pigs (BW 5.02±0.18 ㎏) were allotted into one of five treatments using a completely randomized block design. Treatments were 100:0 (A), 75:25 (B), 50:50 (C), 25:75 (D) and 0 :100 (E) in lactose : sucrose ratio. For the first week after weaning, no significant differences were found in average daily gain (ADG), average daily feed intake (ADFI) and feed conversion ratio (FCR, feed/gain). There were also no significant differences in ADG, ADFI and FCR among treatments during the second and third week after weaning. During overall experimental period, FCR was significantly influenced by dietary treatment, especially B and D treatments showed better FCR than other treatments (p<0.05). During the initial 7 day postweaning (Phase I), the nutrient digestibility was not influenced by the lactose: sucrose ratio except for dry matter (DM) and crude protein (CP). CP digestibility of diet A was significantly higher than those of other diets (p<0.05), but no significant.differences were found among diets B, C, D and E. During the second and third week postweaning (phase II), no significant differences were found in digestibilities of GE, DM, CA and P among treatments. There were no significant differences in nutrients excretion among treatments. These results indicate that sucrose can be well utilized by baby pigs as the source of carbohydrate after 15 days of age. It implies that lactose added in weaner diets could be partially replaced with sucrose.

효모 세포벽으로부터 MANNOPROTEIN 과 β-GLUCAN 의 분리

조광근,최윤재,윤연화,문태현,김성찬,복진덕 한국영양사료학회 1999 韓國營養飼料學會誌 Vol.23 No.6

본 연구는 효모 세포벽으로부터 manoprotein과 β-glucan을 분리하는 방법을 확립하기 위하여 실시하였다. 효모로부터 분리된 manoprotein과 β-glucan은 동물의 면역기능을 강화시키는 기능성 가축용 면역증강제로 개발하기 위한 목적을 가지고 있다. 따라서 본 연구에서는 이러한 목적을 달성하기 위하여 알칼리 추출 원리에 근거한 Catley의 방법 (Yeast, a practical approach, 1988, edited by Campbell and Duffus, IRL Press)을 변형하여 사용하였으며, 효모의 처리는 파쇄한 것과 파쇄하지 않은 것을 이용하여 manoprotein, β-glucan 및 protein 함량과 순도를 조사하였다. 세포건물로부터 추출된 세포벽 함량은 파쇄한 세포와 파쇄하지 않은 세포에서 각각 15.4%와 28.8%로 나타났으며, mannan의 함량은 파쇄하지 않을 경우 단백질을 제외한 mannan의 함량이 28.5%, 파쇄할 경우 52%로 나타나 mannoprotein은 파쇄된 효모에서 높은 추출율을 보였다. 효모세포벽으로 부터 추출된 mannan 순도에 있어서 파쇄된 효모와 파쇄되지 않은 효모에서 각각 97.6%와 77.6%로 나타나 파쇄할 경우 mannan의 추출 순도를 높이는 효과를 보였다. 그러나 glucan 추출율에 있어서는 파쇄할 경우와 파쇄하지 않은 경우 7.2%와 18.2%로 나타나 파쇄하지 않을 경우 추출율이 높게 나타난 경향을 보였으며, glucan의 순도는 70.8%와 71.7%로 비슷한 결과를 나타내었다. 따라서 본 연구에서 확립한 방법은 알칼리 용해성 glucan을 제외하였으므로 실제로 분리할 수 있는 glucan의 함량은 더 높을 것으로 생각된다. 결론적으로 본 연구에서 확립한 방법은 효모 세포벽으로부터 mannoprotein을 분리할 수 있는 효과적인 방법이라고 생각된다. In this study the extraction method of both mannoprotein and β-glucan was established from yeast cell wall fractions. From this method, both mannoprotein and β -glucan was used as an immune potentiator for domestic animals by activating the overall immune activity. Both mannoprotein and β-glucan were extracted from yeast cell wall fractions or total cell using basically Catley's method (Yeast, a practical approach, 1988, edited by Campbell and Duffus, IRL Press) with minor modification and simplification to be fit into an industrial application. With our modified extraction method. the extractable cell wall contents from mechanically broken cells and unbroken cells were 15.4% and 28.8%, respectively. Mannan contents of cell wall fractions from unbroken and broken cells were 28.5% and 52%, respectively, showing much higher extraction efficiency when the yeast cells were mechanically broken. Mannan contents extracted from cell wall fractions from unbroken and broken cells were 77.4% and 97.6%, respectively. For a higher purity of mannan, thus, it is necessary to break up the yeast cells. On the other hand, the extraction of glucan moiety was obtained 18.2% and 7.2% extraction rates from the cell wall preparation of unbroken and broken yeast cells, respectively, with similar sample purity in both samples (71.7%, 70.8%). In this study, alkali-extractable glucan was not included in the extraction process for the sake of simplicity necessary for the industrial application. However if this step is included in our modified protocol, the glucan yield will be significantly increased. In conclusion, the mannoprotein extraction method from yeast cell wall established in this study may be very effective and applicable in the industrial scale.

3 성분계 유기용제의 인화특성에 관한 연구

최재욱,하동명,목연수,임우섭,최일곤,조태제 한국산업안전학회 1999 한국안전학회지 Vol.14 No.3

This study was accomplished by measuring the lower and upper flash point with air blowing method and grasping the characteristics of flammability for the three component systems, which are made up of the Benzene-Toluene-o-Xylene and Methylethylketone-Toluene-o-Xylene. These three component systems are widely used in the various industrial fields together with the development of industry. The results are as follows ; 1) Isothermal line is plotted on the triangular diagram for flash points determined in each solutions. From this line, the mixed compositions which indicated the same lower and upper flash points in each different composition could be read on this diagram, if the composition of mixtures are known. 2) Lower and upper explosion limits obtained from the flash points determined for the three component solution are compared with the value calculated from Le Chatelier's law. Especially the lower explosion limits are in a good agreement with the calculated values.

중탄소 Boron 강의 가스침질탄화처리에 의해 형성된 화합물층의 마모특성에 관한 연구

이상윤,이해우,조효석,이준범,박기원,오도원 한국열처리공학회 1999 熱處理工學會誌 Vol.12 No.2

The study on the wear characteristics of compound layers formed during gaseous nitrocarburising in the medium carbon boron steels and the plain carbon steel has been carried out by using a pin-on-disc type wear test machine under the oil lubricating condition at room temperature and by varying applied loads, sliding speeds and wear distances. Values of friction coefficient measured at the sliding speed of 0.4m/sec under the oil lubricating condition have been shown to decrease considerably with increasing applied load and become gradually a constant value as load is increased to a higher value, showing that the transition load for friction coefficient appears at an applied load of 247.2N. The length and volume wear rates of compound layer have been revealed to relatively constantly increase, also showing that the wear life per unit thickness of compound layer turns out to be superior as porous layer has a denser and thinner appearance. As the sliding speed increases during wear test performed by varying sliding speed at a load of 63.2N under the oil lubricating condition for medium carbon boron steel nitrocarburised in gas atmosphere, the wear rate has been found to increase, the friction coefficient to decrease and the wear life per unit thickness of compound layer to decrease considerably.