대청도 자생 순비기나무 토양환경특성과 번식

조영덕 한국토양비료학회 2006 한국토양비료학회 학술발표회 초록집 Vol.2006 No.10

Simple Maturation of Direct-Converted Hepatocytes Derived from Fibroblasts

조영덕,윤상태,강교진,김요한,이승범,서대관,류기영,정재민,최동호 한국조직공학과 재생의학회 2017 조직공학과 재생의학 Vol.14 No.5

Target cells differentiation techniques from stem cells are developed rapidly. Recently, direct conversion techniques are introduced in various categories. Unlike pluripotent stem cells, this technique enables direct differentiation into the other cell types such as neurons, cardiomyocytes, insulin-producing cells, and hepatocytes without going through the pluripotent stage. However, the function of these converted cells reserve an immature phenotype. Therefore, we modified the culture conditions of mouse direct converted hepatocytes (miHeps) to mature fetal characteristics, such as higher AFP and lower albumin (ALB) expression than primary hepatocytes. First, we generate miHeps from mouse embryonic fibroblasts (MEFs) with two transcription factors HNF4a and Foxa3. These cells indicate typical epithelial morphology and express hepatic proteins. To mature hepatic function, DMSO is treated during culture time for more than 7 days. After maturation, miHeps showed features of maturation such as exhibiting typical hepatocyte-like morphology, increased up-regulated ALB and CYP enzyme gene expression, down-regulated AFP expressions, and acquired hepatic function over time. Thus, our data provides a simple method to mature direct converted hepatocytes functionally and these cells enable them to move closer to generating functional hepatocytes.

전혈과 혈청에서의 칼륨 이상소견 검사의 차이

조영덕,최성혁,윤영훈,박상민,김정윤,임채승 대한수혈학회 2012 大韓輸血學會誌 Vol.23 No.2

Background:Potassium, the most common cation in the intracellular space, plays a critical role in our physiology. Potassium imbalance may cause life-threatening problems, ranging from general weakness to cardiac arrest due to ventricular fibrillation. For emergency physicians, detection of such derangement within a short period of time is of critical importance. In this study, we wanted to determine whether analysis of whole blood samples can be used as a screening tool for potassium imbalance by comparative analysis of whole blood and serum samples. Methods:Two samples were drawn from 227 patients. The whole blood sample was taken from the radial artery and contained in a commercially available arterial blood collection syringe with a lithium-heparin coating. The serum sample was contained in a commercially available vacuum bottle in a non-additive silicone coated tube and transported to the laboratory. The study population was divided into three groups, patients with normal whole blood potassium, patients with decreased whole blood potassium, and patients with elevated whole blood potassium. Potassium levels for each group were coupled with serum potassium levels and compared. Results:No significant difference in potassium values was observed between whole blood and serum samples (P<0.05). Strong associations were observed among the three groups (normal range, hypokalemia, and hyperkalemia group). Compared to the normal group (r=0.851), the hyperkalemia group showed a stronger association between variables (r=0.897), and the hypokalemia group showed a weaker association (r=0.760). Their correlation coefficients were highly significant (P<0.05). Conclusion:Our study illustrates that point-of-care testing using whole blood with whole blood can be a reliable screening tool when treating patients with suspicious potassium abnormality, especially in hyperkalemia patients.

구연 : 소화기 ; 총담관결석제거를 위하여 내시경적 유두부 풍선확장술을 시행한 106예의 임상상 및 결과 분석

조영덕,천영국,김영석,문종호,김연수,이문성,심찬섭,김부성 대한내과학회 2001 대한내과학회 추계학술대회 Vol.61 No.-

Recent Advances in Pancreatobiliary Imaging : Endoscopic Imaging (ERCP, EUS, Cholangiopancreatoscopy)

조영덕 대한소화기학회 2006 대한소화기학회 춘계학술대회 Vol.2006 No.-

응급센터에서의 수혈치료

조영덕,최성혁,윤영훈,김정윤,박상민,임채승 대한수혈학회 2013 大韓輸血學會誌 Vol.24 No.1

Background:Most patients requiring transfusion are admitted to the general ward; however, the number of patients visiting the emergency department for transfusion is increasing. In this study, we reviewed the transfusion therapies that are performed in the emergency department and analyzed their status. Methods:We conducted a retrospective review of the charts of patients who visited the emergency department in our hospital for transfusion therapy from October 1, 2008 to October 30, 2012. We collected and analyzed general information on the patients and divided them into groups according to the number and kind of blood products they received. Results:A total of 4,497 patients visited the emergency department for transfusion therapy during the study period. Among 4,497 patients, 2,925 patients were enrolled in the study and 1,572 patients were excluded. Out of 2,925 patients, there were 1,745 male patients (59.66%) and 1,180 female patients (40.34%), mean age was 61.24 (±17.49); 2,340 patients (80.00%) were admitted, 364 (12.45%) discharged, 44 (1.50%) expired, and 177 (6.05%) were transferred. The most common cause for transfusion was upper and lower gastrointestinal bleeding (928, 32%), followed by trauma (548, 19%), malignancy (376, 13%), anemia (294, 10%), infection (281, 10%), and gynecologic (137, 5%) respectively. Conclusion:Performance of transfusion therapy in the emergency department is not uncommon; therefore, proper protocols by cause of bleeding will be required for prevention of unnecessary complication that may occur during transfusion therapy. 배경: 수혈을 필요로 하는 대부분의 환자들은일반 병동으로 입원하나 최근에는 수혈 치료를위하여 응급센터를 방문하는 환자들의 수가 늘어가고 있다. 이 연구에서는 응급센터에서 수혈치료를 받은 환자들의 현황에 대한 조사 및 분석을하고자 한다. 방법: 본 연구자들은 2008년 10월 1일부터 2012 년 10월 30일까지 수혈치료를 위하여 본원 응급센터를 방문한 환자들의 차트를 후향적 연구로검토하였다. 환자들의 일반적 자료들을 수집했으며 수혈치료의 수와 종류에 따라 여러 그룹으로나눈 후 결과를 분석하였다. 결과: 연구 기간 동안 총 4,497명의 환자가 수혈치료를 받았으며 그 중 1,572명이 제외되고2,925명이 연구에 포함됐다. 총 대상 중 남자1,745명(59.66%), 여자 1,180명(40.34%), 평균나이61.24세(±17.49)였으며 2,340명(80.00%)이 입원, 364명(12.45%)이 귀가, 44명(1.50%)이 사망, 177 명(6.05%)이 전원 조치되었다. 수혈치료를 받은가장 흔한 원인은 위장관 출혈(928명, 32%)이었으며 그 뒤로 외상(548명, 19%), 종양(376명,13%), 빈혈(294명, 10%), 감염(281명, 10%) 그리고 산부인과적 출혈(137명, 5%) 등이었다. 결론: 응급센터에서 수혈치료는 쉽게 접할 수있으며 각각의 원인에 적합한 수혈치료 방법이치료 중 발생할 수 있는 합병증 예방을 위하여 필요할 것이다.

Inversion of the classical Radon transform on $\mathbb{Z}^n_p$

조영덕,현종윤,문성환 대한수학회 2018 대한수학회보 Vol.55 No.6

The Radon transform introduced by J. Radon in 1917 is the integral transform which is widely applicable to tomography. Here we study the discrete version of the Radon transform. More precisely, when $\mathcal{C}(\mathbb{Z}^n_p)$ is the set of complex-valued functions on $\mathbb{Z}^n_p$. We completely determine the subset of $\mathcal{C}(\mathbb{Z}^n_p)$ whose elements can be recovered from its Radon transform on $\mathbb{Z}^n_p$.

The inflammatory response of neutrophils in an in vitro model that approximates the postcardiac arrest state

조영덕,박성준,최성혁,윤영훈,김정윤,이성우,임채승 대한외과학회 2017 Annals of Surgical Treatment and Research(ASRT) Vol.93 No.4

Purpose: Postcardiac arrest syndrome (PCAS) shares many features with sepsis including plasma cytokine elevation with dysregulation of cytokine production, and the presence of endotoxin in plasma. PCAS is closely related to ischemia-reperfusion injury. During ischemia-reperfusion injury, neutrophil, which is the first line of innate immunity, plays a major role. In this study, we investigated the inflammatory response of human neutrophils in an in vitro model which we simulated with hypoxia-normoxia and hypoxia-hyperoxia environments. Methods: After separation of neutrophils from the whole blood, they were divided into 3 experimental groups: normoxianormoxia, hypoxia-normoxia, and hypoxia-hyperoxia groups. The production of H2O2, the expression of Toll-like receptor 4 (TLR4) receptor, and the extent of apoptosis of the neutrophils were checked. Results: The in vitro hypoxia-normoxia and -hyperoxia models, which simulated the PCAS, showed initiation of the neutrophils’ inflammatory reaction by hypoxia insult. Lipopolysaccharide amplifies such inflammation; therefore, prevention of secondary infection may be critical in postresuscitation patients. Temporary hyperoxia following hypoxic insult showed no difference in inflammatory reaction compared with hypoxia-normoxia. Rather, temporary hyperoxia may suppress or minimize inflammation by attenuation of TLR4 receptor. Conclusion: It is well known that continuous hyperoxygenation after successful cardiac arrest harms patients, but temporary hyperoxygenation with 100% O2 in a clinical situation may be helpful

수술이 불가능한 간문부 담관암 환자에서의 광역동 치료 효과

조영덕 대한췌담도학회 2005 대한췌담도학회지 Vol.10 No.2

비록 광역동 치료가 고식적이고 담즙 배액관 삽입과 병행하여 시행하고 있으나 시술과 관련된 뚜렷한 합병증이 없고 반복적 시술이 가능하면 여러 연구에서 삶의 질 및 생존률의 향상이 있어 수술이 불가능한 담관암환자에서 광역동 치료는 뚜렷한 이득이 있는 것은 사실이다. 담관암의 특징이 담도를 따라 종양이 확산되는 특징이 있어 원격성 전이가 없는 Bismuth 3형, 4형에서 광역동 치료는 유용하며 신체적 또는 고령으로 인해 수술을 시행할 수 없는 Bismuth 1, 2형 그리고 하부 담관암환자에서도 광역동치료를 병행하는 것은 유용할 것으로 생각한다. 그러나 좀 더 많은 증례 수에서 전향적 비교 연구 및 장기간 추적 관찰이 광역동 치료의 역할을 규명하는데 필요할 것으로 생각한다.

담석을 동반한 간내 담관암과 담석을 동반하지 않은 담관암의 임상적 비교

조영덕 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.1

Background/Aim: Hepatolithiasis are very common in Korea and hepatolithiasis is a risk factor for cholangiocarcinoma. The differences of clinical, laboratory or radiological features between intrahepatic cholangiocarcinoma associated with hepatolithiasis and cholaniocar-cinoma alone does not well known until now. Author performed this study to define the clinical, laboratory or radiological differences between intrahepatic cholangiocarcinoma associated with hepatolithiasis and cholangiocarcinoma alone. Methods: Sixty-one patients with intrahepatic cholangiocarcinoma associated with or without hepatolithiasis were analyzed retrospectively. There were 32 patients with cholangiocarcinoma associated with intraheaptic stones (group HC) and 29 cholangiocarcinoma patients without intraheaptic stones (group C). In group HC, there were 21 males and 11 females with a mean age of 63.4 years. Group C contained 17 males and 12 females with a mean age of 61.9 years. Results: Patients with cholangiocarcinoma associated with intrahepatic stones had a significantly longer duration of symptoms, a higher frequency of biliary surgery than cholangiocarcinoma alone. In group HC, the main symptoms were abdominal pain, fever, chilling, and jaundice. In group C, the main symptoms were abdominal pain and jaundice. Concordance rate of location of stones and tumor was closely related in 96% of patients with cholangiocarcinoma and hepatolithiasis. Segmental stricture and dilated intrahepatic ducts were the most common radiological features of both groups. Tumor mass observed on the imaging study was encountered significantly frequent in the group C (82% vs. 53%, p<0.05). However, hepatic atrophy was noted more frequent in the group HC than group C (46% vs. 10%, p<0.05). There were no statistical differences between the two groups in the laboratory findings such as liver function test or tumor marker. Preoperative diagnostic rate of cholangiocarcinoma was lower in group HC than group C (78.2% vs. 96.6%). Conclusion: If bile duct stricture or hepatic atrophy are noted in patients with intrahepatic stones, active effort for detection of concomitant cholangiocarcinoma will be needed.