Production and Characterization of Enzymes Involved in Chitin Catabolic Cascade from a Bacterial Strain Isolated from Soil

( Ji Hyun Lee ),( Jeong In Lee ),( So Yeon Park ),( Yoo Kyeong Jang ),( You Bin Lim ),( Hye Yoon Lee ),( Young Min Jung ),( Hae Chang Lee ),( Jong Hwa Lee ),( Shaheen Amna ),( Jae Kweon Park ) 한국키틴키토산학회 2019 한국키틴키토산학회지 Vol.24 No.1

The present study was aimed to isolate the bacteria producing chitinolytic enzymes including chitin deacetylase (CDA) from soil origin. About 24 bacteria were screened for their chitinolytic enzymes producing ability on the basis of colloidal chitin. Amongst one of the most potent isolates designated to JI-02 was further selected as the most prominent chitinolytic and CDAlike enzyme (CDA-X) producer based on the ability of deacetylation of an artificial substrate pNP-acetamilide. The maximum production of CDA-X in crude enzyme from JI-02 was observed in the presence of 1% colloidal chitin at 37°C and pH 6.4 after 120 h of incubation. Potent enzyme activity of CDA-X was determined toward pNP-acetamilide, demonstrating that optimal pH, temperature and ionic strength of the CDA-X were assessed to be 7.0, 52°C and 125 mM, respectively. Unexpectedly, Nacetylglucosamine (GlcNAc) was not fully converted by the action of CDA-X to glucosamine (GlcN) under the established conditions. Further study on enzyme activity toward chitin-oligosaccharides consisting of multi-N-acetylglucosamine (GlcNAc)n, n=2-5 may be necessary to elucidate the mode of action which requires the minimum size of (GlcNAc)n. However, our data suggest that CDA-X can convert chitin to chitosan with the maximum yield of approximately 0.08g/L during the fermentation of the strain in the presence of chitin. Furthermore, we found that crude enzyme showed the significant chitosanolytic activity. Taken together, our data suggest that the strain JI-02 may be a potential candidate as a particular strain for better understating of chitin catabolic pathway.

손마사지가 허혈성 심질환 환자의 불안에 미치는 효과

현경선,이향련,공송심,윤경자,김현섭,김효남,최지원,김운정 성인간호학회 2001 성인간호학회지 Vol.13 No.2

The purpose of this study was to investigate the effect of a hand massage program on anxiety, vital sign and pain in clients with ischemic heart disease. The design utilized for this stuffy was quail-experimental with a nonequivalent control group non-synchronized design The subjects were fifty-four patients, twenty-eight for the experimental and twenty-six for the control group, who were admitted with ischemic heart diease at a cardiac intensive care unfit in K medical center of K university. This study was carried out from May, 1999 to March, 2000. The level of anxiety and pain measured by Visual Analogue Scale, systolic blood pressure, diastolic blood pressure and pulse rate were measured before and after hand massage, the state of Anxiety was measured by the Spielberger (1970) scale at admission and after hand massage for three days. The collected data were processed by using the SPSS PC program and analyzed using χ2-test and t-test. The result of this study are as follows : 1. The scores of VAS anxiety and State of anxiety of the experimental group were lower than those of the control group. 2. The degree of systolic blond pressure, diastolic blood pressure and pulse rate of the experimental group were lower than that of the control group. 3. The score of VAS pain of the experimental group was lower than that of the control group. The results suggested that hand massage can decrease VAS anxiety, State of anxiety, vital signs and VAS pain of patients who were admitted with ischemic heart disease at cardiac intensive care unit Therefore, It Is proposed that hand massage is an appropriate nursing intervention to relieve anxiety of the patients who were admitted with ischemic heart disease at a cardiac intensive care unit.

데이터 글러브를 이용한 손 제스처 기반 3차원 게임 구현

유다연,권차욱,곽지민,백화현,김이조,차경애 대구대학교 정보통신연구소 2010 情報通信硏究 Vol.7 No.1

데이터 글러브(Data Glove)는 손 제스처를 정의하여 시스템을 제어하는 입력장치로 가상현실기술 및 교육, 의료, 산업분야에서 많이 사용되고 있다. 이는 가상현실 속에 존재하는 사물을 현실에서 제어하게 함으로써 사용자에게 마치 현실 세계의 사물을 제어하는 느낌을 주는 장점을 가지고 있다. 이에 본 논문에서는 이러한 데이터 글러브의 장점을 이용하여 3차원 게임에 적용시키는 방법을 제안한다. 먼저 데이터 글러브로 제스처를 정의하고, 트래커(Tracker)와 연동하여 시스템을 구현한다. 구현된 시스템을 실험하기 위해서 OpenGL로 인형 뽑기 게임을 구현하고 사용자가 데이터 글러브를 착용 후 정의된 제스처를 통해 게임을 제어하도록 한다. 본 논문에서 제안한 방법은 사용자들에게 좀 더 높은 몰입감을 제공하며, 향후 게임 산업 발전에도 많은 영향을 줄 것으로 기대한다.

도시거주자의 혈중 카드뮴 농도와 간 및 신장 기능과의 상관성 평가

김호현,임영욱,임종한,양지연,신동천 한국환경독성학회 2003 환경독성보건학회지 Vol.18 No.1

The possibility that liver and kidney function is adversely affected by current levels of environmental exposure to cadmium (Cd) as investigated in adult men and women in the general population in Seoul. From February to August in 2001, blood and morning spot urine samples were collected from 136 not occupationally exposed group (age range 20~75 years) at 4 survey sites throughout Seoul. Liver and kidney function parameters in serum and urine were examined by conventional methods. The questionnaire included factors, i.e. sex, age, smoking, alcohol, diet habit etc. The geometric mean values for Cd in blood (Cd-B) were 1.43 μg/l. It seemed prudent to conclude that liver and kidney function as not disturbed by the current environmental exposure to Cd in study.

비파괴적 분석법을 활용한 활음진 함유 에멀젼의 인체효능평가

배지현,안성연,이해광,안순애,문성준,장이섭 한국비파괴검사학회 2004 학술대회 논문집 Vol.- No.-

최근 한방화장품이 각광을 받으면서 한방에서 말하는 피부개선효과에 대한 적절한 평가법을 찾는 연구가 많이 수행되고 있다. 본 연구에서는 피부 항산화 효과가 우수한 산사자, 당귀, 홍화, 두충, 숙지황의 초임계추출물 (활음진, 活陰眞)의 혈액순환 개선효과를 적외선 열화상 이미지 분석 및 LaserDoppler Perfusion Imager를 이용한 분석을 통해 평가하였다. 평소 손발이 차가운 여성 11명을 대상으로 활음진을 포함한 에멀젼을 1주일간 도포하게 하였으며, 도포전과 1주일간 사용한 후 피부 온도와 혈류량을 비교하였다. 그 결과 얼굴의 피부 온도가 증가하였고, 이마, 눈위, 뺨정면 부위에서 시료 사용 후 유의한 증가를 보였다. 본 연구를 통하여 활음진 성분을 포함한 에멀젼의 혈액순환 개선효과를 확인하였을 뿐만 아니라 한방적 접근을 통한 피부개선효과를 평가하는데 적절한 실험모델을 수립하였다.

매복 정도에 따른 대구치 이소맹출의 치험례

이지현,김지연,박기태 大韓小兒齒科學會 2010 大韓小兒齒科學會誌 Vol.37 No.1

이소맹출은 치아가 비정상적인 위치나 방향으로 맹출하는 것으로 정의되며 구치부에서는 근심측으로 맹출하여 인접치아의 치근 흡수나 조기 탈락을 야기하여 공간 상실을 일으킬 수 있고, 부분 맹출된 경우 치은 하방의 치아우식 위험성이 증가하고 농양을 형성할 수도 있다. 구치부의 이소맹출 가운데 66% 정도는 가역적인 경우로 특별한 치료 없이 자연적으로 정상적인 맹출이 일어나며, 비가역적인 이소맹출의 경우에는 치아의 맹출 상태, 인접치에 의해 매복된 양, 인접치의 동요도, 통증이나 감염 여부 등의 여러 요인을 고려하여 본격적인 치료가 필요하다. 본 논문에서는 비가역적으로 이소맹출된 상악 제1대구치와 하악 제2대구치를 치간이개 고무줄, modified Halterman appliance 그리고 외과적 노출 후 modified Halterman appliance를 이용한 방법 등으로 정상적인 맹출을 유도한 증례를 보고하는 바이다. Ectopic eruption is defined as the eruption of the tooth in an abnormal position or orientation. In the molar region, ectopic eruption may cause distal root resorption and premature exfoliation of the neighbor teeth and uncontrolled space loss is followed. Prolonged partial impaction of the permanent molar may also cause undetected caries or abscess formation of the neighbor teeth. While 66% of ectopically erupting permanent molars are corrected spontaneously without treatment, the treatment is necessary for the irreversible ectopic eruption cases. The optimal treatment approach depends on a number of factors including the clinical eruption status of the molar, amount of enamel ledge and the mobility of the neighbor tooth, and the presence of pain or infection. This case report presents the results of treatment of the ectopically erupting maxillary first permanent molars and mandibular second molars using elastic separators or modified Halterman appliance with or without surgical approach.

사회적 지지의 개념모델

강지연,김현숙 김천과학대학 2001 김천과학대학 논문집 Vol.27 No.-

Social support has been wildly used concept in the field of health science as well as in the social science. A positive relationship between social support and health is generally accepted in the literature. However, the set of dimensions used to define social support is inconsistent. This study was designed to analyse the concept of social support and to propose a conceptual model of social support. Social support has two different dimensions, functional and structural, and the social integration or embededness connects these two dimensions. In the contest of structural dismension support is an interpersonal process embedded in an array of social exchanges. Researchers who cling to this idea have focused on the social network and support system of clients. Functional aspect of social support suggests four of most frequently used defining attributes of the concept: emotional, instrumental, informational and appraisal. This type of researchers recognizing the importance of support for continued functioning and wellbeing, especially in time of stress and crisis. As an intervention strategy, the provision of social support is highly valued in nursing.

농촌 주부의 의복 활용과 구매 행동에 대한 탐색적 연구

이지연,박재옥,조현숙 漢陽大學校 韓國生活科學硏究所 2000 韓國 生活 科學 硏究 Vol.- No.18

The purpose of this study was to investgate the current trends of clothing practical use and buying behavior of rural women. Subjects of this study were 200 women residing in 3 rural areas in Korea. The data were obtained through questionnaires which handled by the trained Interviewers, from June in 1997 to July in 1997. Data were analyzed by frequency, percentage, cross∼tabulation, Chi square test. Based on the results of this study findings were as follows. 1.More than half women in rural areas ware jacket with pants as formal wear and T-shirts with pants as casual wear. 2.Planned purchase is more often the case of formal wear than that of casual. What motivates to purchase formal wear turns out to be 'I don't have one' with constant to 'look comfortable' which is predominant in the case of casual wear The evaluative criteria of clothing was " color and design" in the case of formal wear wheres "comfort and fitting" in the case of casual wear. The buying place is usually a market having near location, big and small. As so women in rural areas prefer relatively chief clothing and payed by cash. They tend to be satisfied with what they purchased. In a few case of dissatisfaction, they exchange or return the formal wear, but they repair the casual wear. 3.In the case of formal wear, the price or the ratio of discount is the most important store selection criteria and the store display is the most important information source. Also, they usually purchase clothing for ceremony in the case of formal wear. In the case of formal wear, age makes difference on place and time however buying place is dependent upon income. Education level, on the other hand, makes significant difference in buying time. In the case of casual wear, age, education, income makes difference on buying price, evaluative criteria.

유치열기 3급 부정교합 환아에서 facemask의 효과와 재발 양상

김지연,유승은,이지현,박기태 大韓小兒齒科學會 2009 大韓小兒齒科學會誌 Vol.36 No.3

본 연구의 목적은 유치열기 3급 부정 교합 환아에서 facemask를 이용한 악정형 치료 후 골격적 변화와 치료 후 나타나는 재발 양상을 평가하는 것이다. 유치열기 3급 부정교합 환아 15명을 대상으로 구내장치로 bonded expander, 구외장치로 facemask를 이용한 악정형 치료를 평균 12개월 동안 시행하였으며, 1년 간의 follow up 기간 동안 유지 장치는 사용되지 않았다. 치료 시작 전, 치료 직후, 치료 1년 후에 측면두부방사선사진을 촬영하고 전후방 및 수직적 골격관계와 연조직의 변화를 비교분석하였다. 모든 환아에서 치료 직후, 유의할 만한 골격적 전후방 관계의 변화를 보였고 1년 간의 follow-up 기간 동안 재발되는 경향을 보였으나 치료 시작 전과 비교하여 치료 효과는 유지되었다. 수직적 골격적 변화는 치료 직후 증가되었으나 1년 간의 follow up 기간 동안 다시 감소하여 치료 시작 전과 비교하여 차이를 보이지 않았다. 연조직의 변화는 facial convexity 및 상순의 위치가 치료 직후 개선됨을 보였고 1년 간의 follow-up 기간 동안에도 치료 전과 비교하여 치료효과는 유지되었다. 하순의 위치는 치료 직후에 유의할 만한 변화를 보이지 않았다. Facemask는 유치열기 3급 부정교합에 있어서 효과적인 치료 방법이며, 안정적인 치료 결과를 위해서는 적절한 형태의 유지장치가 고려되는 것이 바람직하다. The purpose of this study was to evaluate skeletal and soft tissue changes that occur after using a facemask for treatment of skeletal class Ⅲ malocclusion. and to assess the relapse pattern when no retention appliance was used. Fifteen skeletal class Ⅲ malocclusion patients were treated with a facemask for an average of 12 months. No retention appliance was used during the 1 year follow-up period. Cephalograms were taken during pretreatment, posttreatment, and the 1 year follow-up. Cephalograms were traced, analyzed, and the results were compared between cephalograms. All patients showed significant sagittal skeletal changes after treatment, but they also showed a significant relapse during the 1 year follow-up period when no retention appliance was used. Despite the relapse, the sagittal skeletal changes that remained were still significant. Vertical skeletal change was also significant after treatment, but the total change was not significant after a 1 year follow-up due to relapse. In soft tissue changes, facial convexity and upper lip position improved after treatment and this change remained significant after the 1 year follow-up period. Facemask therapy is therefore an effective method for treatment of skeletal class Ⅲ malocclusion, however, retention is imperative to maintain the treatment effect.

噴霧法에 依한 硫化亞鉛 薄膜의 電場發光 調査

손연규,윤지홍,권태향,한광수,이자현 안동대학교 기초과학연구소 1991 基礎科學 硏究論文集 Vol.2 No.1

The study of Electroluminescence has been begun with that the two Germans, B. Gudden & R. W. Pohl in 1920 examined the remaining lights by dint of adding Electric Field at the fluorescent substance, and from 1952 the study became brisk, in 1960 made a peak, and afterward it was diminished. The above facts were appeared through H. E. Ivey`s investigation. The cause was it that the light-efficiency and the brightness were very pessimistic, though the beginning of EL-Study was given in the development of illumination. In 1950, however, with the initiative that C. W. Jerome of Sylvania Co. deviced and announced EL-Lamp, EL-Fluorescent substances were embed and merchandised as EL-Panels, and after a while it was applied to EL-Lamps, Expression Apparatuses, Light Amplifier, Logic Circuit and so on, with which the use frequency got high, and it has been developed as a promising Electronic Industry. But in our country, considering the various conditions, in order to find out the possibility of EL-Production within the home country, Impurity was introduced to ZnS powder, EL of ZnS : Cu, Cl & ZnS : Cu, Mn, Cl could be manufactured, and surveying the Voltage Characteristics, the Frequency Characteristics, and the Spectral Response of the manufactured things, the fundamental technique-problems about the composition of ZnS powder, the introduction of impurity, the formation of thin-thickness, and the construction of Cell, etc were solved gradually. 1. The quantity of Cu must commonly be more one order than in the use of Photoluminescence, as a whole 10(-3)gm,atm/mole ZnS is fitted, and Mn suitable in about 10 times of Cu. 2. Yellow color must dope Cu as well as Mn, when the quantity of Cu fixed to a certain amount, in case that the quantity of Mn is decreased, it be turned into Yellow color, if it is increased, it be taken Orange color. 3. In vacuum flowing HzS gas and baking at the moment, it is said that EL of which brightness is high, but in the air we can secure ZnS : Cu, Cl EL. only. 4. In referring to the conditions 1, 2, 3, I can infer that EL-manufacture if possible in our country.