Effects of Growth Regulators on Shoot Regeneration and Polysaccharide Production of Orostachys japonicus Berger

김운정 한국약용작물학회 2004 韓國藥用作物學會誌 Vol.12 No.5

Optimal culture conditions for efficient in vitro propagation and polysaccharide production of Orostachys japonicus were established. O. japonicus was cultured in media containing various growth regulatars and carbon sources. The highest regeneration rate was achieved in 1.0 and 3.0 ㎎ ℓ-¹of 2,4-D concentration, while the lowest was obtained in 10.0 ㎎ ℓ-¹2,4-D concentration. When different carbone sources were added in the culture medium, plant growth was high in 3% sucrose treatment. The micropropagated shoots were successfully acclimatized in artificial soils aid produced comparable amont of polysaccharide compred to parent cultivated plants.

Production of Tropane Alkaloids by Two-stage Culture of Scopolia parviflora Nakai Adventitious Root

김운정 한국약용작물학회 2004 韓國藥用作物學會誌 Vol.12 No.5

Scopolia parviflora Nakai, a rare and endangered species, is the sole plant producing tropane alkaloids (TA) among the Korean native species. In order to enhance TA productivity the SP72 root line was selected by screening 100 of root line, and the optimal culture media for root growth and TA production were investigated with SP72 roots. Based on the several media, SH and 2B5 medium were determined as growth medium and White and NN medium as production medium. Among the four combinations of two-stage culture, 2BN (2B5 as growth medium Plus NN as production medium) showed more enhanced root growth and TA production as compared with production media of White and NN medium and growth media of SH and 2B5 medium respectively. However, bubble column bioreactor (BCB) cultures applying two-stage culture did not reveal the effective results despite of the each successful operation of two-stage culture in conical flasks and BCB cultures.

어휘 의미성 및 철자 규칙성에 따른 실어증 환자의 명사 소리내어 읽기 특징

김운정(Woon Jeong Kim),성지은(Jee Eun Sung) 한국언어청각임상학회 2017 Communication Sciences and Disorders Vol.22 No.1

배경 및 목적: 의미성 및 규칙성에 따른 읽기 과제에서 실어증 환자군과 정상군의 명사형 자극 읽기 수행률을 비교하고 그 특징을 살펴보고자 한다. 방법: 실어증 환자 20명과 정상 성인 20명을 대상으로 하였으며, 의미성 및 규칙성에 따라 규칙 단어, 불규칙 단어, 규칙 비단어, 불규칙 비단어 조건에서 2음절 명사형 읽기 자극을 선정하였다. 대상자는 제시된 목표단어를 보고 소리내어 읽도록 지시하였으며, 최종 반응을 전사하여 정반응률을 계산하고 오류 유형을 분석하였다. 결과: 의미성 및 규칙성에 따른 2음절 명사형 자극 읽기에서 실어증 환자군이 정상군에 비해 유의하게 낮은 정반응률을 보였다. 또한 의미성에 따른 집단 간 읽기 수행률의 차이가 유의하였다. 반면 규칙성에 따른 집단 간 차이 및 규칙성 및 어휘성에 따른 집단 간 차이는 유의하지 않았다. 실어증 중증도를 예측하는 변인은 규칙 비단어로, 약 47.7%의 예측력을 가지는 것으로 나타났다. 오류 비율은 비단어가 단어에 비해 2배 이상 높았으며, 비단어 조건에서 더 많은 규칙화 오류가 나타났다. 논의 및 결론: 실어증 환자는 후천적인 뇌손상으로 인해 읽기 결함을 보이며, 빈도가 통제되었을 때 규칙성에 비해 의미성에 따른 영향을 더 크게 받는다. 즉 실어증 환자군은 전반적으로 단어에 비해 비단어의 읽기 수행력이 저조한 특징을 보이며, 이는 음운성 실독증의 유형과 유사하다. 또한 철자-음소 전환 과정이 필요한 규칙 비단어가 실어증 중증도를 예측하는 변인이다. Objectives: The purpose of the study was to evaluate the reading ability of persons with aphasia (PWA) according to lexicality and regularity. Methods: Twenty individuals with aphasia and 20 age- and education-matched normal individuals participated in the study. Experimental stimuli were manipulated based on the lexicality and regularity of two-syllable structures at word-level. Accuracy (%) served as a dependent measure, and the types of error were analyzed. Results: For the reading accuracy, PWA performed significantly worse than the control group. Participants demonstrated greater difficulties in reading non-words and irregular words across the groups. The two-way interaction between group and lexicality was significant, indicating that PWA demonstrated greater difficulties in reading non-words than words compared to the control group. Regression analyses revealed that regular non-word condition accounted for 47.7% of the total variance as a significant predictor. In the error type analyses, the error ratio of regular non-word condition was 71.74%, which was greater than that of word condition, 28.26%. Regularization error was 14.75% in non-word condition and 9.36% in word condition. Conclusion: These results indicated that PWA demonstrated greater reading difficulties than the control group, and their performance was differentially affected by the lexicality. Lexicality seems to play a critical factor in differentiating PWA from the normal control group. The results are consistent with previous findings that suggested that non-word reading was more difficult than words. The current study suggested that overall severity of aphasia was predicted by regular non-word condition, which placed greater processing burden on grapheme-to-phoneme conversion.

지역아동센터 종사자의 전문성 인식과 직무만족

김운정(Woon-Jeong Kim),최유석(Youseok Choi) 한국콘텐츠학회 2014 한국콘텐츠학회논문지 Vol.14 No.2

이 연구의 목적은 지역아동센터 종사자의 전문성 인식과 직무만족 간의 관계를 밝히는 것이다. 이를 통해 지역아동센터 관리자와 실무자의 전문성과 직무만족도를 증진함으로써 서비스의 질을 향상시킬 수 있는 방안을 모색하고자 하였다. 본 연구에서는 서울, 경기, 강원지역의 지역아동센터 시설장 및 생활복지사 221명을 대상으로 설문조사를 수행하였다. 조사결과, 응답자의 전문성 인식은 중간 이상의 수준을 보였으며, 직무만족도는 중간보다 조금 높은 수준을 보였다. 직무내적 만족도에 비해, 직무외적 만족도가 낮게 나타났다. 특히 보상만족도는 가장 낮게 나타났다. 회귀분석결과, 전문성 인식과 그 하위영역인 전문적 지 식/기술, 전문적 가치인식이 높을수록 직무만족도, 직무내적 만족도, 직무외적 만족도 모두 높은 것으로 나타났다. 이러한 연구결과를 토대로 지역아동센터 종사자의 전문성을 향상시킬 수 있는 정책적, 실천적 시사점과 향후 연구과제를 제시하였다. This study examines the effects of community child care center workers’ perception of professionalism on their job satisfaction. This study conducts a survey of 221 community child care workers in Seoul, Gyeonggi, and Gangwon provinces in Korea. Results show that the level of perception of professionalism and job satisfaction are over intermediate. The perception on professional values and ethics are slightly higher than the perception on professional knowledge and skills. The workers are more satisfied with intrinsic job factors such as achievement and responsibility than extrinsic job factors such as compensation and working conditions. Regression analysis reveals that the workers’ perception of professionalism is positively associated with both intrinsic and extrinsic job satisfaction. Based on the findings, this study provides suggestions to enhance the workers’ professionalism and job satisfaction, which will contribute the quality of services in community child care centers.

고지를 이용한 바이오에탄올 생산

김운정(Un-Jeong Kim),김미선(Mi-Seon Kim),신수정(Soo-Jeong Shin) 한국신재생에너지학회 2014 한국신재생에너지학회 학술대회논문집 Vol.2014 No.11

産後風에 對하여

金雲貞 대한한의학회 1985 대한한의학회지 Vol.6 No.1

Dopant-Dependent Electrical and Thermoelectric Properties of Selenophene Contained Conjugated Polymer

윤상은,한지민,정구현,배온유,김동욱,김운정,서형탁,김선주,김기출,김봉기,김종현 한국고분자학회 2022 한국고분자학회 학술대회 연구논문 초록집 Vol.47 No.2

식용 버섯과 식물성 유지의 혼합급여가 흰쥐 간장의 지질 성분 및 지방산 조성에 미치는 영향

김군자(Goon-Ja Kim),김한수(Han-Soo Kim),김성희(Sung-Hee Kim),김희숙(Hee-Sook Kim),최운정(Woon-Jeong Choi),정승용(Seung-Yong Chung) 한국식품영양과학회 1994 한국식품영양과학회지 Vol.23 No.5

식용버섯과 식물성유지의 혼합급여가 고콜레스테롤혈증 흰쥐 간장의 지질성분 및 지방산 조성에 미치는 영향을 구명하기 위해 Sprague-Dawley계 숫 흰쥐에게 10% 돈지와 5% cellulose 식이군을 대조군으로 하고 영지버섯, 표고버섯 및 목이버섯을 5%씩 그리고 올리브유, 홍화유 및 들깨유를 10%씩 상호 혼합한 식이를 급여하여 3주간 실험사육한 후 간장의 지질성분 및 지방산 조성을 분석 검토한 결과는 다음과 같다. 간장의 총콜레스테롤 농도는 3군 (올리브유 10%+표고버섯 5%)과 6군 (홍화유 10%+표고버섯 5%)이 대조군 및 여타 실험군에 비해 유의적으로 낮았고 중성지질의 농도는 8군 (들깨유 10%+영지버섯 5%) 및 9군 (들깨유 10%+표고버섯 5%)이 낮았으며 인지질의 농도는 3군, 5군 (홍화유 10%+영지버섯 5%), 6군, 7군 (홍화유 10%+목이버섯 5%), 8군, 9군 및 10군 (들깨유 10%+목이버섯 5%)이 대조군에 비해 유의적으로 낮았다. 간장 총지질의 지방산 조성에서 MUFA의 함유비율은 2군 (올리브유 10%+영지버섯 5%) 3군, 4군 (올리브유 10%+목이버섯 5%) 및 전들깨유군에서 높았고 PUFA 및 LA의 함유비율은 전홍화유군에서 현저히 높게 나타났다. 간장 인지질의 지방산 조성은 PUFA의 함유비율이 높았고 MUFA는 낮았으며, 중성지질에서는 MUFA의 함유비율이 SFA보다 약간 높았고, 콜레스테롤 에스테르에서는 MUFA의 함유비율이 현저하게 높았다. 간장 지질성분 모두 PUFA 중 n-6계 LA의 함유비율이 가장 높았으며 인지질 성분에서 n-3계 EPA의 함유비율이 월등히 높게 나타났다. This study was designed to observe the effects of the mixed diets of edible mushrooms and vegetable oils on the lipid component and fatty acid composition in liver of the diet induced hypercholesterolemic rats. Ten groups of male S.D. rats were fed a basal diet supplemented with 5% of one of three mushrooms (Gl, L.e, A.j) and 10% of one of three vegetable oils (olive, safflower, perilla) for three weeks. In liver, total cholesterol concentration was significantly low in groups 3 (olive oil 10%+L. edodes 5%) and 6 (safflower oil 10%+L. edodes 5%), triglyceride concentration was low in groups 8(perilla oil 10%+G. lucidum 5%) and 9 (perilla oil 10%+L. edodes 5%) and phospholipid concentration was significantly low in groups 3, 5(safflower oil 10%+G. lucidum 5%), 6, 7 (safflower oil 10%+A. judae 5%), 8, 9 and 10 (perilla oil 10%+A. judae 5%). In the fatty acid composition of total lipid in liver, monounsaturated fatty acid (MUFA) concentrations were high in groups 2 (olive oil 10%+G. lucidum 5%), 3 and 4 (olive oil 10%+A. judae 5%) and all the perilla oil groups, polyunsaturated fatty acid (PUFA) and linoleic acid concentrations were significantly high in all the safflower oil groups. In the fatty acid composition of liver phospholipid, PUFA concentrations were high but MUFA concentrations were low. In the triglyceride component, MUFA were some more than saturated fatty acid (SFA). In the ch. olesteryl ester component, MUFA concentrations were significantly high. In the fatty acid composition of liver lipid components, linoleic acid was high in the PUFA and so it was major fatty acid. Eicosapentaenoic acid (EPA) of phospholipid component in liver was significantly high.

서울 지역 노인의 영양 상태에 관한 연구

김정형,김주영,서경아,서정원,이숙희,정혜정,조운정,한재정,한성숙,오경원,이혜련,박소미,채선희,최윤정 연세대학교 생활과학대학 1986 婦學 Vol.19 No.-

A survey was carried out to determine the nutritional status of rural elderly. The subjects of this study comprised of eighty­seven persons aged sixty years and over in Seoul area. The questionnaire was disigned to investigate the environmental status of the subjects. At the same time, twenty­four hour dietary recall method was used to estimate their nutrient intakes. The results are summerized as follows: ㆍ Mean body weight and height of subjects were 53.3±12.7㎏ and 153.3±1.6㎝ respectively. ㆍ The chief somatic complains of subjects were fatigue(75.9%), amnesia(66.7%), rheumatism(65.5%) and Joint pain of muscleache(64.4%). ㆍ Most of subjects(74.4%) answered that their appetite changed with increasing age. ㆍ Daily nutrient intakes of subjects were sufficient as from 120 to 150% of RDA. ㆍ The nutritional status of subjects including energy, protein, vitamin and mineral intakes was proven to be improved than the results obtained from the earlier studies.

아마인유와 해바라기 종자유의 혼합 급이가 식이성 고지혈증 흰쥐 혈청 지단백의 지방산조성에 미치는 영향

최운정(Woon-Jeong Choi),김한수(Han-Soo Kim),정효숙(Hyo-Sook Cheong),김군자(Goon-Ja Kim),서인숙(In-Sook Su),정승용(Seung-Yong Chung) 한국식품영양과학회 1994 한국식품영양과학회지 Vol.23 No.1

n-3계 PUFA와 n-6계 PUFA의 배합 비율을 달리한 혼합유지가 고지혈증 흰쥐의 지질개선 및 지방산 조성에 미치는 영향을 구명하기 위해 Sprague-Dawley계 숫 흰쥐에 돈지(3.0%), 버터(12.0%) 및 콜레스테롤(0.75%)을 첨가 조제한 식이를 3주간 급여하여 고지혈증을 유발시킨 후, 돈지 3.0%와 버터 12.0%식이를 대조군으로 하고 아마인유와 해바라기 종자유를 사용, 그 배합 비율을 달리한 혼합유지 및 항고지혈증 약제를 급여하여 2주간 실험 사육한 후 혈청 지단백의 지방산 조성을 분석한 결과, 각 성분 모두 C_(18:2)의 함유비율이 높았으며, C_(20:5)의 비율은 LDL과 HDL성분에, C_(22:6)은 chylomicron 성분에서 그 비율이 높았고, n-3P/n-6P비는 5군에서 9군으로 감에 따라 저하되는 경향으로서 대체로 시험유지의 지방산 조성이 반영된 것으로 나타났다. This study was carried out to investigate the effect of the feeding mixture of linseed oil, rich in n-3 PUFA and the sunflower seed oil, rich in n-6 PUFA on the lipid metabolism in the dietary hyperlipidemic rats. After male Sprague-Dawley rats were induced hyperlipidemia by feeding the diet containing lard, butter and cholesterol for 3 weeks, then they were fed with the diet containing lard 3.0% and butter 12.0% for control, the mixture in different proportion of both linseed oil and sunflower seed oil and antihyperlipidemic drugs for 2 weeks. Analysis of the fatty acid composition of the serum lipoprotein fractions showed following results. In the fatty acid composition of serum lipoprotein, the proportion of C_(18:2) was dominant in all fractions, C_(20:5) in LDL and HDL fraction and C_(22:6) in chylomicron fraction. The ratio of n-3P / n-6P tended to increase gradually as it of the test lipid increased in groups 5 to 9 group and was affected by the fatty acid composition of the test lipids.