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세포질내 정자주입법(ICSI)에 있어서 정자흡입 및 난자내 주입방법에 관한 연구
이택후,김항진,송건호,김대근,전상식,박윤규,서태광,전병균,류은경,이은숙,문진수,김광철 경북대학교 의학연구소 2000 경북대학교병원의학연구소논문집 Vol.4 No.1
Study on Method of Sperm Aspiration and Injection into an Oocyte in Intracytoplasmic Sperm Injection(ICSI) Immobilization of spermatozoa prior to intracytoplasmic sperm iniection(ICSI) sometimes results in crooked tail and this makes it difficult to aspirate sperm into an injection pipette tail first. Head-first sperm aspiration into an injection pipette avoid this problem due to the bigger size of the sperm head. The effect of head or tail-first sperm injection into an oocyte on fertilization cleavage, percentage of grade I embryos and development to blastocyst stage in ICSI program has been studied. A single living immobilized spermatozoa from oligoasthenozoospermic patient was injected into an oocyte head-first or tail-first according to the treatment. Eighteen hours after microinjection, oocytes ware inspected for survival and fertilization Fertilized oocytes with two pronuclei were cultured in 30μl drop of mHTF supplemented with 10% heat-inactivated follicular fluid(FF) at 37℃. On day 2. embryo transfer was performed with cleaved embryos. The remaining 2-8 cell stage embryos were co-cultured with BRL cells in mHTF + 10% FF for 72 hours and the developmental stage was observed. The data were analyzed by Analysis of Variance. A total of 164 oocytes from 36 cycles were assigned to earth treatment and ICSI was performed(88 head-first, tail-first). The rates of normal fertilization were 81.8% and 76.3% for head-first and tail-first, respectively. Of the fertilized oocytes, the percentage of cleaved embryos and the percentage of grade 1 embryo among cleaved embryos were 88.9% and 68.8%, 93.1% and 74.1% for head-first and tail-first, respectively. Of the 2-8 cell embryos cultured, 44.4%(16/36) and 50.0%(10/20) for head first and tail first, respectively developed to blastocyst stage. There were no differences in fertilization, cleavage, rates of grade 1 embryos, and development to blastocyst stage. In conclusion, head-first or tail-first sperm injection into an oocyte in ICSI program does not affect fertilization and subsequent embryo development to blastocyst stage in vitro.
정철영,고재관,황원채,윤희철,박가열,김재호,이성식,김동승,표성일,이건남,양안나,최수정,김은석 한국직업능력개발원 2008 직업능력개발연구 Vol.11 No.1
이 연구의 목적은 제대군인에 대한 효율적인 전직지원을 위해서 현행 전직지원 프로세스에 대한 개선 안과 이에 따른 국방부, 국가보훈처 등 제대군인 전직지원관련 기관 간의 역할분담 및 연계방안에 대해서 제안하는 것이다. 연구는 크게 세 가지 과정을 거쳤다. 첫째, 국내 군 전직지원 실태를 분석하여 문제점과 시사점을 도출하였다. 둘째, 이러한 문제점과 시사점, 군 전직지원 선행연구를 종합하여 효율적인 군 전직지원 프로세스 안을 제시하였다. 셋째, 구축한 군 전직지원 프로세스 안에 따라 주요 군 전직지원 관련 기관인 국방부, 국가보훈처의 역할 분담 안을 제안하였다. This study aims to suggest an improvement plan for the support process of effective occupation change for discharged soldiers and then role assignments among related institutions for supporting discharged soldiers such as Ministry of Defense, Ministry of Patriots and Veterans Affairs, Ministry of Labor and the cooperation plan among the institutions. The study was made in three steps. First, after analyzing currents support facts and status for discharged soldiers, it induced its problems and implications. Second, integrating the findings of previous studies related to support plan on occupation changes for the discharged soldiers, Problems and implications mentioned above, it constructed effective occupation change process for discharged soldiers. Then, according to the established process, it suggested role assignments such as Ministry of Defense, Ministry of Patriots and Veterans Affairs, Ministry of Labor.
Vein of Galen 기형 환아에서 중재적 방사선하 혈관내 색전술시의 마취관리 경험
박성식,임동건,한규석,홍정길,김용선 대한마취과학회 2001 Korean Journal of Anesthesiology Vol.41 No.3
Anesthetic Management during Interventional Neuroradiologic Embolization of a Vein of Galen Malformation - A case report - Kyu Seok Han, M.D., Sung Sik Park, M.D., Dong Gun Lim, M.D. Yong Sun Kim, M.D.^*, and Jung Gil Hong, M.D. Department of Anesthesiology and ^*Diagnostic Radiology, School of Medicine, Kyungpook National University, Daegu, Korea The vein of Galen malformation is a midline intracranial arteriovenous fistula with aneurysmal dilatation of the vein of Galen. This abnormality, though rare, is associated with a bleak prognosis and significant mortality. However, significant advancements have been made in understanding the path-ophysiology of this abnormality, and improvements in both interventional neuroradiologic embolization and microneurosurgical techniques have contributed to a better outcome. In neuroradiologic embolization, children are likely to become agitated and confused and thereby jeopardize the entire procedure. For this reason, this procedure is performed with the children under general anesthesia. We experienced a case of neuroradiologic embolization under general anesthesia with propofol. The patient was 9-year-old boy who weighed 29 kg. He was healthy and had no other abnormalities in our preoperative evaluation. Throughout the entire procedure, especially before and after closure of the fistula, hemo-dynamic changes and unexpected events did not appear. (Korean J Anesthesiol 2001; 41: 388~392)
Undifferentiated endometrial sarcoma mimicking submucosal myoma: A case report
( Gun Sik Park ),( Hyun Sik Youm ),( Soo Jin Song ),( Byeung Jum Kim ),( Mi Eun Jung ),( Sang Chill Kwon ),( Sang Kook Kim ),( Yu Kyung Cho ),( Ja Sung Koo ),( Hwal Woong Kim ),( Hwa Sook Moon ) 대한산부인과학회 2012 대한산부인과학회 학술대회 Vol.98 No.-
Endometrial stromal tumors are the second most common uterine sarcomas. They are divided into three types; endometrial stromal nodule, endometrial stromal sarcoma (ESS), and undifferentiated endometrial sarcoma (UES). UES is much less common than ESS. And it has a much more aggressive clinical course and poorer prognosis than ESS. Case: A 47-year-old woman presented to our hospital with a 4-month history of irregular vaginal bleeding. TVUS revealed a vascularized mass mimicking submucosal myoma in the uterine endometrial cavity. MRI was performed. A 5.9 × 5.1 × 4.8 cm submucosal mass filled up the endometrial cavity. Hysterocopy examination confirmed the protruding mass at the lower portion of the endometrial cavity. The frozen section of the hysteroscopy was reported as malignancy. Subsequently, the patient underwent laparoscopic hysterectomy with bilateral salpingo-oophorectomy and bilateral pelvic lymph node dissection. There was no cancerous involvement in 19 dissected lymph nodes. The results of immunohistochemical stainings were positive for vimentin and CD56, a few positive for CD68, and negative for smooth muscle actin, desmin, CD10, CD34, pan-cytokeratin, HMB-45, S-100 protein, chromogranins and synaptophysin. Based on these histomorphologic findings, the diagnosis of UES was made. The patient was referred to an oncological center for chemotherapy and radiation therapy. Conclusion: It is important to establish a proper classification of endometrial stromal tumors by cytologic and immunohistochemical findings becase of different clinical course and prognosis.