서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1998)

김재홍,김준호,반재용,이정우,황성주,정준규,정성태,강진문,조흔정,홍창의,정혜신,이한승,김이선,이봉길,이종호,선영우,한기덕,윤성필,이성훈,안종성,박석범,문승현,조항래,김형섭,류지호,황재영,박준홍,손상욱 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1

In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria gonorrhoeae(PPNG), we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by menas of the chromogenic cephalosporin method. In 1998, 93 strians of N. genorrhoeae were isolated, among which 60(64.5%) were PPNG. The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 64.5% in 1998.

경막내 척수외 종양의 수술적 치료

신병준,이재철,윤태경,정호원,한성우,김동원,김경제,김연일 대한척추외과학회 2002 대한척추외과학회지 Vol.9 No.3

파킨슨씨병에서 Selegiline HCI (MAO-B^(�))의 효과 및 안전성

조기현,김명규,김태선,김병채,이승한,황인용,김준태,김종기 全南大醫大 2000 전남의대학술지 Vol.36 No.2

고콜레스테롤혈증 환자에서 Atorvastation의 유용성 및 안전성에 관한 연구

고종훈,신준한,김한수,탁승제,최병일,김동수,권혁문,김현승 대한순환기학회 1999 Korean Circulation Journal Vol.29 No.9

Distribution of Aquaporins (Water Channels) in the Rat Salivary Glands

정지연(Ji-Yeon Jung),한창룡(Chang-Ryoung Han),정연진(Yeon-Jin Jeong),오원만(Won-Mann Oh),김미원(Mi-Won Kim1),김선헌(Sun-Hun Kim),김옥준(Ok-Joon Kim),김현진(Huyn-Jin Kim),고정태(Jeong-Tae Koh),최홍란(Hong-Ran Choi),김원재(Won-Jae Kim) 대한해부학회 2002 Anatomy & Cell Biology Vol.35 No.3

침샘은 하루 1.5 l의 침을 분비하며, 일반적으로 침샘에서 수분이동은 능동적 염분 이동에 따른 삼투현상에 의해 일어난다. 따라서 침샘에서 높은 수분투과성으로 인해 수분통로 단백인 aquaporin (AQP)들이 풍부하게 존재할 수 있다. 지금까지침샘에 4가지 형태의 AQP이 존재한다고 알려져 있지만 침샘에서 정확한 위치와 수분 이동에 대한 각 AQP들의 역할은 아직 확실치 않다. 본 연구는 300 g 정도의 흰쥐를 pentobarbital sodium (50 mg/kg, IP) 마취하에서 carbarchol (10 μg/kg)을 복강 내 투여한 후 AQP들의 역할과 분포를 면역조직화학방법으로 조사하였다. AQP1은 침샘의 미세혈관의 내피세포와 샘세포와 샘관 주위에 존재하는 근육상피세포에 존재하였다. AQP4는 침샘관의 마지막 부위인 배출관에 존재하였다. AQP5는 주로 장액세포의 바닥가쪽과 세포사이 분비소관을 포함한 세포꼭대기쪽막 에 존재하였으며 콜린성 분비 자극으로 세포꼭대기쪽막으로 이동되어 밀집되었다. AQP5는 모든 침샘의 사이관과 줄무늬 관에도 존재하였으며 점액세포는 약하게 존재하였다. AQP8은 AQP5처럼 장액세포의 바닥쪽막과 세포사이 분비소관을 포함한 세포꼭대기쪽막에 존재하였으며 콜린성 침분비 자극으로 세포꼭대기쪽막쪽으로 이동되어 밀집되었다. 이상의 실험결과는 AQP5와 AQP8이 장액세포에서 일차 침 형성시 일어나는 수분이동의 주된 통로임을 시사하였다. The salivary glands produce 1.5 l of fluid per day. As in other organs, the general paradigm in the salivary glands is that water movement occurs secondary to osmotic driving forces created by active salt transport. Therefore, high water permeability in salivary glands is expected to need a variety of aquaporin (AQP), a water channel. Although four AQPs have been known to reside in salivary glands, the precise location and roles of AQPs have been not well examined. This study is aimed to investigate the distribution of AQPs in 3 major salivary glands and their changes after cholinergic stimulation using immunohistochemical study in Sprague Dawley rats weighing 300 g under pentobarbital sodium anesthesia. AQP1 was localized in the endothelial cells of all salivary capillary vessels and the myoepithelial cells. AQP4 was demonstrated in the epithelium of the excretory ductal cells of all salivary glands. AQP5 and 8 were abundantly present in the basolateral membrane and apical membranes of the serous acini including intercellular secretory canaliculi, whereas AQP5 was weakly present in mucous acini. In addition, AQP5 was found in the epithelium of the intercalated and striated ducts. Upon stimulation of carbachol (10 μg/kg, I.P). AQP5 and 8 tended to translocate from basolateral membrane to the apical membrane, appearing as clusters of dots. These results suggest that AQP5 and 8 are the candidate molecules responsible for the water movement in salivary acinar cells.

韓國의 肝吸蟲症에 관한 臨床的 硏究

金名世,李駿商,林漢鍾 고려대학교 의과대학 1982 고려대 의대 잡지 Vol.19 No.1

For understanding of the significance on clinical features of clonorchiasis, the authors carried out the examinations for hematology, blood biochemistry and clinical symptoms on the patients whose stools were positive eggs of clonorchis sinensis in several endemic areas in Korea. A total of 287 clonorchiasis patients were examined with the stools by Stoll's egg counting method for the intensity of infection. The patients were divided into light, moderate Ⅰ & Ⅱ, heavy and very heavy infection groups depending upon the number of eggs per gm, of feces(EPG). All the calculated mean values for each items with standard deviation of hematological and blood bichemical tests were compared with those of the normal values and the detailed clinical symptoms according to the intensity of infection were analyzed. The results obtained are as follows: 1. The mean values of hematologic findings in each group of infection were within the normal limit except eosinophil counts. In the changes of hematological values by means of intensity of infection, the mean values of hemoglobin, hematocrit, RBC, WBC and eosinophil counts were increased in accordance with the intensity of infection. 2. To observe the occurrence of anemia in the patients infected with C. sinensis, the mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) were compared in each group of infection. But all the values were shown within normal value. 3. All the mean values of blood biochemistry in each group of infection were within the normal limit. However the mean values of GPT, GOT, alkaline phosphatase, bilirubin and cholesterol were increased in accordance with the intensity of infection. Whereas, the mean values of total protein, albumin and A/G ratio were decreased. 4. The subjective symptoms of human clonorchiasis were increased by the higher intensity of infection. Among chief complaints, asthenia, lassitude and mental depression were most common as generalized symptoms, but as gastrointestinal symptoms, abdominal discomfort, epigastric pain and indigestion were most frequent. In physical examination, hepatomegaly, epigastric tenderness and jaundice were found most frequently. Even in the light infection group, over 60 per cent of patients complained the subjective symptoms. 5. With the above results, the changes in the fields of hematology, blood biochemistry and the clinical symptoms by means of intensity of infection were discussed for the specific clinical features of clonorchiasis.

HPLC를 이용한 농약 Carbendazim의 측정법에 관한 연구

김준서,조만희,이상한,김창세 순천향의학연구소 1997 Journal of Soonchunhyang Medical Science Vol.3 No.1

The measurements of pesticide Carbendazim were studied and following conditions were proposed as a most good methods; 1. There were detected at 204 nm by means of Hitachi 200-20 spectrophotometer. 2. Pesticide Carbendazim mesurement was performed by menas of ISCO model 2350, spherisorb ODS column, 70% acetonitrile as a solvent phase, flow rate 0.5 ml/min, chart speed 0.5 cm/min, and 25℃ oven temperature. 3. The relationships between the concentration and absorbance of pesticide Carbendazim were linear from 2.5 to 15 ppm. 4. It showed stable result in Folin-Wu, and the recovery rate was also good.

복합재료 이론에 의한 2차원 상판 구조물의 정확하고 간단한 해석

김준민,한봉구 서울산업대학교 2005 논문집 Vol.54 No.2

대부분의 건설기술자들은 강판형교나 콘크리트 교량을 단위 폭을 가진 보로 해석 하나 이러한 구조물은 엄연히 복합재료로 이루어진 판(plate) 구조이다. 이러한 구조물은 균등단면에 등분포하중을 받고 4변 단순 지지된 경우가 아니면 정확한 해석이 불가능하다. 복합 재료의 이론은 일반 기술자들이 이해하기 힘드나, 본 논문에서는 이러한 문제를 비교적 쉽게 정확히 해석하는 방법을 제시하고, 예로서 3경간 연속 판 교량과 포스트텐션된 강교의 해석을 보여준다.

肝吸蟲症에 있어서 尿를 이용한 免疫學的 診斷에 관한 硏究

金榮容,李駿商,林漢鍾 고려대학교 의과대학 1985 고려대 의대 잡지 Vol.22 No.3

Human clonorchiasis is one of the most common trematode disease and of very important public health problems in Korea. In this study, the enzyme-linked immunosorbent assay (ELISA) was employed in the diagnosis by use of urine samples in human clonorchiasis. ELISA was performed with veronal buffered saline antigen of Clonorchis, peroxidase conjugated antirabbit IgG and orthophenylenediamine as a substrate by micro-method. The results obtained are as follows; 1. The mean O.D. (optical density) values of ELISA in the urine samples of non-infected human cases was 0.037±0.087. If we proposed the O.D. value 0.1 as a differential point of positive reaction, the specificity ELISA in the urine samples was 93% (187 negative reaction out of 201 non-infected human cases). 2. The mear. O.D. valuses in the urine samples of clonorchiasis was 0.580±0.574 and in the serum samples of clonorchiasis was 1.930±0.623. The sensitivity of ELISA was 87.0% in the urine samples (409 positive reaction out of 470 clonorchiasis cases) and was 88.4% in the serum samples. 3. In the distribution of the O.D. values in the urine samples of clonorchhiasis, false positive rates of cross reaction between C. sinensis and P.westermant were 34.7% in the urine samples of clonorchiasis. 4. When the results were compared between the urine and the serum samples of 181 clonorchiasis showed that 88.7% gave same reactions. In the clonorchiasis cases, the O.D. values between the urine and the serum simples were significant at a level of p<0.01. 5. In rabbits, the O.D. values of the urine and the serum samples in 16weeks after infection with C. sinensis metacercariae to were greatly increased than those of the urine samples in preinfection. The O.D. values in the urine samples increased accoording to intensity and course of infection. In conclusion, results of this study suggest that ELISA using the urine samples is more applicable in the diagnosis of human clonorchiasis in mass screening.

췌장의 대낭성 장액성 낭선종 : 2예 보고

김한성,최현준,주미,장선희,곽지은,김철남 인제대학교 2006 仁濟醫學 Vol.27 No.-

Typical serous cystadenoma of pancreas is formed by many microcysts, of which diameter are less than 2cm. Recently, rare variants are also reported as macrocystic, unicystic, or multicentric. Macrocystic serous cystadenoma is diffcult to differentiate from pseudocyst, mucinous cystadenoma and other cystic mass having macrocysts. As these lesions have different symptoms, methods of treatment, and prognoses, it is important to diagnose and treat correctly. We report 2 cases which diagnosed as macrocystic serous cystadenoma with stressing scarcity value and clinical importance.