Human clonorchiasis is one of the most common trematode disease and of very important public health problems in Korea.
In this study, the enzyme-linked immunosorbent assay (ELISA) was employed in the diagnosis by use of urine samples in human clonorc...
Human clonorchiasis is one of the most common trematode disease and of very important public health problems in Korea.
In this study, the enzyme-linked immunosorbent assay (ELISA) was employed in the diagnosis by use of urine samples in human clonorchiasis.
ELISA was performed with veronal buffered saline antigen of Clonorchis, peroxidase conjugated antirabbit IgG and orthophenylenediamine as a substrate by micro-method.
The results obtained are as follows;
1. The mean O.D. (optical density) values of ELISA in the urine samples of non-infected human cases was 0.037±0.087.
If we proposed the O.D. value 0.1 as a differential point of positive reaction, the specificity ELISA in the urine samples was 93% (187 negative reaction out of 201 non-infected human cases).
2. The mear. O.D. valuses in the urine samples of clonorchiasis was 0.580±0.574 and in the serum samples of clonorchiasis was 1.930±0.623. The sensitivity of ELISA was 87.0% in the urine samples (409 positive reaction out of 470 clonorchiasis cases) and was 88.4% in the serum samples.
3. In the distribution of the O.D. values in the urine samples of clonorchhiasis, false positive rates of cross reaction between C. sinensis and P.westermant were 34.7% in the urine samples of clonorchiasis.
4. When the results were compared between the urine and the serum samples of 181 clonorchiasis showed that 88.7% gave same reactions.
In the clonorchiasis cases, the O.D. values between the urine and the serum simples were significant at a level of p<0.01.
5. In rabbits, the O.D. values of the urine and the serum samples in 16weeks after infection with C. sinensis metacercariae to were greatly increased than those of the urine samples in preinfection.
The O.D. values in the urine samples increased accoording to intensity and course of infection.
In conclusion, results of this study suggest that ELISA using the urine samples is more applicable in the diagnosis of human clonorchiasis in mass screening.