水晶體 α-Crystallin의 Aggregation에 對한 Calcium의 影響에 關한 硏究

郭明憲,林圭,郭相太,黃炳斗 충남대학교 의과대학 지역사회의학연구소 1987 충남의대잡지 Vol.14 No.2

The elution profile of CaCI_2 treated and EGTA plus CaC1_2-treated soluble fraction protein of bovine lens are compared by Sephacryl S-300 chromatography, and then changes of protein patterns of CaCI_2-treated crystallin and EGTA plus CaCI_2-treated α-crvstallin fractions have been investigated by urea/polyacrylamide gel electrophoresis. 1. In the presence of 0.8 mM CaCI_2, 10 mM CaCI_2, or 10 mM CaCI_2 plus 20 MM EGTA, absorbance at 500 nm exhibited 0.039, 0.087, 0.040 respectively. 2. Physiologic concentration (0.8 mM) CaCI_2-treated, 10 mM CaCI_2-treated and 10 mm CaCI_2 plus 20 mM EGTA-treated soluble fractions were analyzed by Sephacryl S-300 chromatography. In the 0. 8 mM CaCI_2-treated proteins four peaks were eluted in the tube No. 17, 27, 31, 35 and identified as α, β_H, β_L, γ-crystallin, but eluted in the tube No. 16, 26, 30, 33 in 10 mM CaCI_2-treated protein, respectively. In presence of 10 mM CaCI_2 plus 10 mM EGTA, protein patterns exhibited the same. 3. Physiological concentration (0. 8 mM) CaC_2-treated α-crystallin was seperated ten subtractions by urea/polyacrylamide gel electrophoresis. In the 10 mM CaCI_2-treated α-crystallin, No. 9 subfraction was disappeared and No. 3B was newly appeared when compared with that of 0.8 mM CaCI_2 treated and 10 mM CaCI_2 plus 20 mM EGTA. 4. Absorbance and electrophoretic pattern of 10mM CaCI_2-treated native a-crystallin exhibited the same result of 0.8 mM CaCI_2-treated. 5. Ten inilimoles mercaptoethanol treated soluble fraction protein exhibited the same elution profile when compared with nontreated soluble fraction proteins. From the above results, it is suggested that the aggregation of a-crystallin of lens by calcium may be resulted to the interaction of (β,γ-crystallin, and not related the formation of disulfide bond.

가토뇌 Microtubule의 효소적 Carboxylmethylation이 Microtubule 기능 및 성상에 미치는 영향

곽명헌,권기량,임규,황병두 충남대학교 의과대학 지역사회의학연구소 1991 충남의대잡지 Vol.18 No.2

Microtubule purfied from rabbit brain by 2 cycles of polymerization and depolymerization in the presence of 2 mM GTP and 20% glycerol was carboxyl methylated by a purified protein methylase Ⅱ of rabbit brain, and changes in the properties of microtubule by carboxylmethylation were inverstigated. Tubulin and microtubule associated proteins were endogenously and exogenously carboxelmethvlated by protein methylase Ⅱ in a rate of 28.6 and 71.7 pmoles of methyl group incorporated into 1 mg protein for 15 minutes, respectively. Microtubule was found to be a better substrate than histone for this enzyme. Microtubule carboxylmethylation by protein methylase Ⅱ was activated 8%, 16% , 6%, 15% and 34% by 2 mM GDP, 2 mM Mg^2+, 2 mM Ca^2+, 40 μM colchicine and 40 μM podophyllotoxin, respectively. The binding of colchicine and Ca^2+ to microtubule were slightly enhanced by carboxylmethlated of microtubule. Polymerization kinetics of carhoxvlmethylated and noncarhuxylmethylated microtubule were similar. The inhibitory action of colrhicine and Ca^2+ on the microtubule polymerization was potentiated by carboxylnethylation of microtubule. These results were discussed with regard to the possible role of protein carboxylmethylation by protein methylase Ⅱ in the regulation of microtubule function.

급성하벽심근경색에서 전흉부유도 ST절 하강의 의의

정병천,배호상,곽동훈,김영태,배용학,황종현,임현주,류재근,조용근,채성철,전재은,박의현 慶北大學校 醫科大學 1997 慶北醫大誌 Vol.38 No.2

목적 : 급성하벽심근경색증 환자에서 심전도상 흔히 동반되는 전흉부유도 ST절 하강의 의의를 알아보았다. 방법 : 심근경색후 24시간에서 48시간에 얻은 심전도를 기준으로 52명의 급성하벽심근경색증 환자를 전흉부유도에서 ST절의 하강이 있는 군과 없는 군으로 나누었으며 심도자, 관상동맥조영술, 심초음파도 및 방사성 핵종을 이용한 좌심구혈율 측정 등을 시행하였다. 결과 : 1) 좌심실확장기말압은 전흉부유도에서 ST절의 하강이 있는 경우에서 16.9±5.33mmHg로 없는 경우의 11.0±4.02mmHg보다 유의하게 높았다(p<0.05). 그러나 운동량 및 좌심구혈을은 흥부 유도상 ST절의 하강이 있는 경우 7.6±3.0 METs 및 53.9±9.1%로 없는 경우의 9.1±2.7 METs 및 54.6±9.5%보다 낮았으나 통계적 유의성은 없었다(p=0.180, p=0.823). 2) 관상동맥조영술상 전흉부 ST절의 하강이 동반된 군에서는 유의한 좌전하행지나 좌회선지의 협착이 있는 경우가 60.9% 및 57.9%로 ST절의 하강이 없는 군에서의 34.5% 및 7.7%와 비교해 좌관상동맥에 협착이 동반된 경우가 많았다(p=0.050, p=0.001). 3) 관상동맥조영술상 세 혈관질환의 빈도는 흉부유도상 ST절의 하강을 보인 23예에서 10예로 ST절 하강이 없었던 29예중 2예에 비하여 유의하게 많았고(P=0.007), 단 혈관질환의 빈도는 ST절의 하강을 보인 군에서 23예중 5예로 ST절 하강이 없었던 29예중 17예에 비하여 유의하게 적었다(P=0.002). 두 혈관질환의 경우에는 각각 8예와 10예로 양군간에 유의한 차이가 없었다. 4) 흉부유도상 ST절 하강의 빈도는 단 혈관질환 22예 중 5예에서, 두 혈관질환 18예중 8예, 세 혈관질환 12예 중 10예에서 관찰되어 협착이 있는 관상동맥의 수가 많을수록 ST절의 하강이 나타나는 빈도가 많음을 보였다(p=0.00308). 그러나 흉부유도상 나타난 ST절의 하강을 모두 합하여 구한 ST절 하강의 합은 협착이 있는 관상동맥의 수와 통계적인 유의성이 없었다. 5) 심초음파도상 좌심실 하벽의 벽운동은 ST절 하강을 보인 군에서는 akinesia가 83.3%, hypokinesia가 11.1%, 정상 벽운동이 5.6%로, ST절 하강이 없었던 군에서의 dyskinesia가 3.6%, akinesia가 39.3%, hypokinesia가 25.0%, 정상 벽운동이 32.1%와 비교하여 ST절의 하강이 있는 군에서 유의하게 벽운동의 장애가 빈번함을 보였다(p=0.03891). 그러나 좌심실전벽, 측벽 및 중격부위에서는 유의한 벽운동의 차이가 없었다. 결론 : 급성하벽심근경색증 환자의 전흉부유도에서 ST절의 하강이 동반되는 경우 다혈관 질환의 가능성이 높으며 좌심실확장기말압이 상승되었고 운동능력은 감소되어 있었다. 또한 심초음파도상 좌심실하벽의 벽운동장애도 심하였다. 따라서 전흉부 ST절의 하강을 동반한 급성하벽심근경색증 환자는 전흉부유도에 ST절 하강이 없는 경우보다 예후가 불량할 것으로 생각되며, 보다 적극적인 치료가 필요할 것으로 생각된다. The purpose of this study was to determine the significance of precordial ST segment depression appearing during acute inferior myocardial infarction and to assess the correlation of that with angiographic finding. 52 patients were allocated into two groups based on the existence of precordial ST segment depression : 23 patients with ST segment depression and 29 patients without ST segment depression. The extent of coronary artery disease as well as the prevalence of significant stenosis (≥70%) in left anterior descending artery were more frequent in patients showing precordial ST segment depression than in patients not showing them (p=0.050) and the result were similar in cases of left circumplex artery (p=0.001). On echocrdiographic examination of left ventricular (LV) wall motion, it showed more severely decreased inferior LV wall motion in patients showing precordial ST segment depression than in patients not showing them (p=0.03891) even though the other segment of LV wall motion didn't show the statistical difference between both groups. The other cardiac parameters such as LVEDP, exercise amount (METs) and ejection fraction were tend to showing bad profile in patients with precordial ST segment depression. From the above results, we could deduce that patients with precordial ST segment depression have relatively large infarction or concomitant left coronary artery disease, so they need more caution and intensive theraphy.

개에서 이중 결찰법을 통한 동맥관 개존증의 완치 예

윤헌영,정순욱,박희명,박철,정만복,김준영,한현정,황민,노병국,박상혁,장하영,박정윤 한국임상수의학회 2004 한국임상수의학회지 Vol.21 No.1

A ten months old, female Yorkshire terrier weighing 2.88 kg referred to veterinary teaching hospital of college of veterinary medicine, Konkuk University because of syncope, cough and dyspnea. First hematological and serum chemical test revealed thrombocytopenia, mild anemia, and increase of concentration of ALP (195 U/L). On 57 days later, second hematological and serum chemical test revealed polycythemia, increase of concentration of ALP (211 U/L), and Tchol (387 mg/dl). Right atrium enlargement, main pulmonary artery bulge and cardiomegaly (VHS = 11.5) were observed in radiographic findings. Ultrasonographic images showed both right and left ventricular dilation and turbulent flow between the descending aorta and the main pulmonary artery in color Doppler imaging. ECG showed left ventricular enlargement, SA block, and electrical alternant. Thoracotomy was performed through left fourth intercostal incision under isoflurane anesthesia. Patent ductus arteriosus was double ligated with 1-0 silk. Cough and dyspnea disappeared on 5 days after operation. Turbulent flow was not found in color doppler imaging of ultrasonography on 10 days after operation. Ten months later after the operation, syncope could not exist any more.

胎盤組織 Guanine Aminohydrolase에 關한 硏究

유지헌,林圭,郭相太,黃炳斗 충남대학교 의과대학 지역사회의학연구소 1987 충남의대잡지 Vol.14 No.2

Guanine aminohydrolase(EC 3.5.4.3, GAH) has been purified from human term placenta approximately 1,280-fold with a 10% yield by combination of ultracentrifuge, ammonium sulfate precipitate, Sephacryl S-300 chromatography, calcium phosphate gel adsorption, DEAEcellulose chromatography and hydroxyapatite chromatography, and then its properties were investigated. 1. The purified enzyme appeared single protein band by SDS/polyacrylamide gel electrophoresis. 2. The enzyme showed a pH optimum around 5.6-8.6. It was heat labile, inactivated by heat-treatment for 10 minutes at 70℃, and irreversively denatured by freezing and was stable at 4℃ for at least 1 month. 3. Copper ion (Cu^2+) was a potent inhibitor, the activity being inhibited upto 75% at 2 mM concentraion and the inhibition was completely recovered its activity by additon of 4 mM EDTA, and zinc ion (Zn^2+ also inhibited upto 40% at 2 mM concentration. The other ions did not almost inhibit. 4. The apparent Km value for guanine was 4 X 10^-5M, and the enzyme activity was completely inhibited by 0. 5 mM of p-hydroxymercuribenzoic acid, but all of the activity was recovered in adding 10 mM mercaptoethanol. 5. The molecular weight of the enzyme showed 170,000 by Sephacryl S-300 chromatography, and 55,000 by SDS-polyacrylamide gel electrophoresis. And the properties such as above results of guanine aminohydrolase of human term placenta are compared with those of guanine aminohydrolase of other tissues.

살모사 교상 후에 발생한 뇌간 경색

권석범,이병철,배재천,황성희,김대훈 대한신경과학회 1998 대한신경과학회지 Vol.16 No.4

오존을 이용한 하수슬러지의 감량화와 안정화

이창근,황은주,강성재,빈정인,이병헌 한국물환경학회 2004 한국물환경학회지 Vol.20 No.3

In this study, ozone was adopted for the reduction and stabilization of waste sludge from the municipal sewage treatment plant. The waste sludge used in the experiments was primary sludge, excess sludge and a mixture of the two. The sludge cells and flocs were disrupted by ozonation resulting in the reduction of TSS and VSS concentrations. In the case of the primary sludge with a concentration of 20 gTS/L, the TSS and VSS concentrations were reduced 28%, 33% and the TCOD concentration was reduced 20% respectively. The consumption of ozone was 0.18 gO₃/gSS. In the case of the excess sludge with a concentration of 7.5 gTS/L, the TSS and VSS concentrations were reduced 37%, 41%, and the TCOD concentration was reduced 19% respectively. The consumption of ozone was 0.33 gO₃/gSS. In the case of the mixed sludge of 9.3 gTS/L, the TSS and VSS concentrations were reduced by 45%, 53%, and the TCOD was reduced 26% respectively. The desirable consumption of ozone was 0.27 gO₃/gSS. As ozonation proceeded, the level of SCOD increased due to the release of intracellular materials. However TCOD was reduced due to mineralization resulting from the transformation of organic materials to inorganic materials.

서울의 Penicillinase Producing Neisseria Gonorrhoeae 발생빈도(1996)

김재홍,황동규,전재홍,김윤석,김중환,김용준,이창균,임동진,김현수,조창근,김경문,박상훈,전우형,김희성,이호정,차명수,김갑형,김형석,김석우,황지환,박병순,권오상,이민수,송기훈,성소영,이인섭,부태성 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.2

Background : In recent years, gonorrhea has been panedemic and remains one of the most commom STDs in the world, especially in developing countries. Objective & Methods: For the detection of a more effective therapeutic regimen and assessing the prevalence of PPNG, we have been trying to study the patients who have visited the VD Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results: In 1996, 139 strains of N. gonorrhoeae were isolated, among which 53(39.0%) were PPNG. Conclusion: Our results suggests that after a peak of 74.3% in 1993, the prevalence of PPNG in Seoul is gradually declining.

상수도 배수방식의 비교방법

박남식,홍성훈,김한수,황병은 동아대학교 건설기술연구소 2003 硏究報告 Vol.27 No.1

In this study quantitative and systematic methods are proposed to compare alternative water supply pipe networks. The design code gives general guidelines on hydraulics and power requirements. However, the design code lacks detailed approaches. In this study aspects regarding hydraulics, water quality, and power requirements are considered. Various quantitative indices are derived.

백서에서 Depulpin®과 Formocresol에 대한 치수와 치근단 조직의 반응

문형인,김선호,황윤찬,오병주,황인남,김선헌,정선와,윤창,오원만 大韓齒科保存學會 2002 Restorative Dentistry & Endodontics Vol.27 No.4

One fifth dilution of formocresol is usually for pulpotomy of the primary teeth and emergency pulpotomy of the permanent teeth. However, the use of formaldehyde has been subjected to criticism because it may be absorbed into the blood stream and become distributed systemically, it may also alter the pulp tissue rendering it immumologically active, and have carcinogenic potential. Recently Depulpin®(VoCo., Germany) gains popularity as a devitalizing agent during root canal therapy in spite of high concentration of 49% paraformaldehyde because it facilitate devitalization of pulp and make root canal therapy easier. But there have been not enough publications about the reaction of pulp and periapical tissue caused by Depulpin. This study was performed to evaluate the histological changes in pulp and periapical tissue of rats after pulpotomy using formocresol and Depulpin and to elucidate the toxic effects of these agents. Thirty six Sprague-Dawley rats were anesthetized by intraperitoneal injection of ketamine. Maxillary first molar teeth were used for pulpotomy with formocresol and Depulpin. Rats were sacrificed after 2 days, 4 days, 1 week, 2 weeks, 3 weeks and 4 weeks respectively. Specimens were histologically observed by light microscope changes in pulp and periapical tissue. The obtained results were as follows. 1. Formocresol group A zone of fixed tissue, in which odontoblasts could clearly be defined, was present directly underneath the pulpotomy dressing in almost all teeth of this group. This was followed by an area of necrotic tissue which resembled dried out fibrous tissue with no cellular detail except some pyknotic nuclei. In the specimens of after 2 days, 4 days, 1 week, 2 weeks in which vital tissue was present. it was separated from the fibrous area by a zone of inflammation. In the specimens of after 3 weeks and after 4 weeks, inflammatory infiltrate was in the periodontal ligament adjacent to the apical foramina of the teeth. 2. Depulpin® group The area of necrotic tissue which had no cells and fibers , was present adjacent to the dressing. This was followed by dried out fibrous tissue with no cellular details except some pyknotic nucleli. A short stump of vital pulp with odontoblasts was present at the end of the canal after 2 days. Inflammatory infiltrate was in the periodontal ligament after 4 days and after 1week. Severe root resorption and necrosis of periapical tissue opposite the root resorption site were defined after 2 weeks and after 3 weeks. Periapical lesion which consist of necrotic tissue surrounded by a fibrous connective wall. was found after 4 weeks. The results indicated that Depulpin can cause more adverse reaction to the dental pulp and periapical tissue than formocresol, and further studies are needed for its clinical use with safety.