생강과 생강나무의 향기성분조성 비교

문형인,이재학 韓國作物學會 1997 한국작물학회지 Vol.42 No.1

생강나무의 향기성분 조성을 분석해 본 결과는 다음과 같다. 1. GC상에서 생강나무는 꽃, 잎, 줄기 각각 60, 80, 83개의 peak가 관찰되었으며, 주요 성분군은 생강나무의 경우는 꽃의 경우는 sabinene, eta -myrcene, 1-limonene, cir-3-hexanal, λ -terpinene, (Z)-3-hexen-1-ol acetate, λ -elemene, 1-borneol, δ -guaiene, ledene, δ -ca-dinene, elemol, 9-octadecanal, 1-(1,5diNe-4-hexenyl ) -4-mebenzene, α -chamigrene, λ -selinene, β-endesmol, 1-phellandrene, 3-Me-6-(1-Me, ethyl ) -2-cyclohexen-1-one, epiglobulol의 성분이, 잎의 경우는 phellandrene, α -terpinolene, sabinene, β-myrcene, ι-limonene, cis-3-hexanal, λ -terpinene, (Z)-3-hexen-1-ol acetate, λ -elemene, 1-borneol, δ -guaiene, ledene, δ -cadinene, olemol, 9-octadecanal, λ -selinene, o-chamigrene, 1-(1,5-diMe-4-hexenyl) -4-mebenzene, β-endesmol의 성분이, 줄기의 경우는 sabinene, β-myrcene, ι-limonene, phellamdrene, α -terpinene, ledene, 1-borneol, λ -terpinene, 2,4a,5,6,7,8,9,9a-octahydroben-zocycloheptane, elemol, λ -selinene, cis-3-hexanal, (Z)-3-hexen-1-ol acetate, λ -elemene, δ -guaiene, δ -cadinene, 9-octadecanal, 1-(1,5-diMe-4-hexenyl) -4-mebenzeno, β-endesmol, α -charugrene의 향기성분이 주요 성분군으로 확인되었다. 2. 생강나무에서 생강의 향기를 발산하는 성분으로는 β-myrcene, o-terpinolene, phellandrone, ι-limonene, β-eudesmol, δ -cadinone, elemol, trans-caryophyllene으로 동정되었으며 그 중에서도 phellandrene, β-eudesmol이 주된 역할을 하는 성분으로 확인하였다. The composition and chemical structures of same individual components of essential oils from ginger flavor plants were estimated by gas chromatography and gas chromatography-mass spetrometric analysis with the aid of NBS and Wiley library and RI indice searches. Through gas chromatography and gas chromatography /mass spetrometry analysis of 43, 41, 32 essential oil components from flowers, leaves and stems from Lindera obstusiloba., respectively were identified, among which sabinene, β -myrcene, ι-limonene, phelandrene, ~gamma -selinene, α -terpinene, 2, 4a, 5, 6, 7, 8, 9, 9a -octahydro benzocycloheptane, δ -cadinene, ~gamma -terpinene, (Z) -3-hexen-1-ol acetate, ~gamma -elemene, l-boreneol, δ -guaiene, ledene, cis-3-hexanal, elemol, α -chamigrene, β -endesmol: 9-octadecanal, 1-(1, 5-diMe-4-hexenyl)-4-Me. benzene were estimated to be major components.

꽃치자 지하부의 식물화학적 성분

문형인,오좌섭,김종식,진배준,지목표 한국생약학회 2002 생약학회지 Vol.33 No.1

벼의 항산화성분

문형인,민병선,이형규,지옥표 한국생약학회 2002 생약학회지 Vol.33 No.3

그늘쑥의 식물화학적 성분

문형인,권학철,지옥표,이강노,Moon, Hyung-In,Kwon, Hak-Cheol,Zee, Ok-Pyo,Lee, Kang-Ro 한국생약학회 1999 생약학회지 Vol.30 No.1

Two carotenoids and four flavonoids were isolated from the whole plant of Artemisia sylvatica Max. Their structures were identified as ${\beta}-carotene$, lutein, luteolin, rutin, $pectolinarigenin-7-O-{\beta}-glucopyranoside$ and luteolin-7-O-neohesperidoside on the basis of spectroscopic means.

백서에서 Depulpin^®과 Formocresol에 대한 치수와 치근단 조직의 반응

문형인,김선호,황윤찬,오병주,황인남,김선헌,정선와,윤창,오원만,Moon, Hyung-In,Kim, Sun-Ho,Hwang, Yun-Chan,Oh, Byung-Ju,Hwang, In-Nam,Kim, Sun-Hun,Jeong, Sun-Wa,Youn, Chang,Oh, Won-Mann 대한치과보존학회 2002 Restorative Dentistry & Endodontics Vol.27 No.4

One fifth dilution of formocresol is usually used for pulpotomy of the primary teeth and emergency pulpotomy of the permanent teeth. However the use of formaldehyde has been subjected to criticism because it may be absorbed into the blood stream and become distributed systemically, it nay also alter the pulp tissue rendering it immunologically active, and have carcinogenic potential. Recently Depulpin$^{\circledR}$(VoCo., Germany) gains popularity as a devitalizing agent during root canal therapy in spite of high concentration of 49 % paraformaldehyde because it facilitate devitalization of pulp and make root canal therapy easier But there have been not enough publications about the reaction of pulp and periapical tissue caused by Depulpin. This study was performed to evaluate the histological changes in pulp and periapical tissue of rats after pulpotomy using formocresol and Depulpin and to elucidate the toxic effects of these agents. Thirty six Sprague-Dawley rats were anesthetized by intraperitoneal injection of ketamine Maxillary first molar teeth were used for pulpotomy with formocresol and Depulpin. Rats were sacrificed after 2 days, 4 days, 1 week, 2 weeks, 3 weeks and 4 weeks respectively. Specimens were histologically observed by light microscope changes in pulp and periapical tissue. The obtained results were as follows. 1. Formocresol group A zone of fixed tissue. in which odontoblasts could clearly be defined, was present directly underneath the pulpotomy dressing in almost all teeth of this group. This was followed by an area of necrotic tissue which resembled dried out fibrous tissue with no cellular detail except some pyknotic nuclei. In the specimens of after 2 days, 4 days, 1 week, 2 weeks in which vital tissue was present, it was separated from the fibrous area by a zone of inflammation. In the specimens of after 3 weeks and after 4 weeks, inflammatory infiltrate was in the periodontal ligament adjacent to the apical foramina of the teeth. 2. Depulpin$^{\circledR}$ group The area of necrotic tissue which had no cells and fibers, was present adjacent to the dressing. This was followed by dried out fibrous tissue with no cellular details except some pyknotic nuclei, A short stump of vital pulp with odontoblasts was present at the end of the canal after 2 days. Inflammatory infiltrate was in the periodontal ligament after 4 days and after 1week. Severe root resolution and necrosis of periapical tissue opposite the root resorption site were defined after 2 weeks and after 3 weeks. Periapical lesion which consist of necrotic tissue surrounded by a fibrous connective wall, was found after 4 weeks. The results indicated that Depulpin can cause more adverse reaction to the dental pulp and periapical tissue than formocresol, and further studies are needed for its clinical use with safety.

백서에서 Depulpin®과 Formocresol에 대한 치수와 치근단 조직의 반응

문형인,김선호,황윤찬,오병주,황인남,김선헌,정선와,윤창,오원만 大韓齒科保存學會 2002 Restorative Dentistry & Endodontics Vol.27 No.4

One fifth dilution of formocresol is usually for pulpotomy of the primary teeth and emergency pulpotomy of the permanent teeth. However, the use of formaldehyde has been subjected to criticism because it may be absorbed into the blood stream and become distributed systemically, it may also alter the pulp tissue rendering it immumologically active, and have carcinogenic potential. Recently Depulpin®(VoCo., Germany) gains popularity as a devitalizing agent during root canal therapy in spite of high concentration of 49% paraformaldehyde because it facilitate devitalization of pulp and make root canal therapy easier. But there have been not enough publications about the reaction of pulp and periapical tissue caused by Depulpin. This study was performed to evaluate the histological changes in pulp and periapical tissue of rats after pulpotomy using formocresol and Depulpin and to elucidate the toxic effects of these agents. Thirty six Sprague-Dawley rats were anesthetized by intraperitoneal injection of ketamine. Maxillary first molar teeth were used for pulpotomy with formocresol and Depulpin. Rats were sacrificed after 2 days, 4 days, 1 week, 2 weeks, 3 weeks and 4 weeks respectively. Specimens were histologically observed by light microscope changes in pulp and periapical tissue. The obtained results were as follows. 1. Formocresol group A zone of fixed tissue, in which odontoblasts could clearly be defined, was present directly underneath the pulpotomy dressing in almost all teeth of this group. This was followed by an area of necrotic tissue which resembled dried out fibrous tissue with no cellular detail except some pyknotic nuclei. In the specimens of after 2 days, 4 days, 1 week, 2 weeks in which vital tissue was present. it was separated from the fibrous area by a zone of inflammation. In the specimens of after 3 weeks and after 4 weeks, inflammatory infiltrate was in the periodontal ligament adjacent to the apical foramina of the teeth. 2. Depulpin® group The area of necrotic tissue which had no cells and fibers , was present adjacent to the dressing. This was followed by dried out fibrous tissue with no cellular details except some pyknotic nucleli. A short stump of vital pulp with odontoblasts was present at the end of the canal after 2 days. Inflammatory infiltrate was in the periodontal ligament after 4 days and after 1week. Severe root resorption and necrosis of periapical tissue opposite the root resorption site were defined after 2 weeks and after 3 weeks. Periapical lesion which consist of necrotic tissue surrounded by a fibrous connective wall. was found after 4 weeks. The results indicated that Depulpin can cause more adverse reaction to the dental pulp and periapical tissue than formocresol, and further studies are needed for its clinical use with safety.

수종의 작물에 대한 세포독성효과

문형인 한국자원식물학회 1997 한국자원식물학회지 Vol.10 No.4

식용 및 약용작물중에서 암세포에 대하여 생체세포독성을 유발하는 소재를 선발하기 위하여 한국인종양에서 유래한 대장암세포주(SNU-C4)와 위아세포주(SNU-1)에 대해 MTT검색법으로 50% 세포성장저해농도($IC_{50}$)를 측정한 결과는 다음과 같다. 1. 암세포에 대해 생체세포독성활성을 가지는 작물을 선발하기 위하여 17종의 작물을 대상으로 검색한 결과 보리, 옥수수, 오미자에서 세포독성효과가 $230{\mu}g/ml$이하의 농도로 유의한 효과를 나타내었다. 2. 품종간의 세포독성효과의 차이를 검색하기 위하여 세포독성효과를 나타낸 작물중에서는 보리와 옥수수를, 세포독성효과를 미약하게 나타내거나 나타내지 않은 작물중에서는 벼와 귀리를 대상으로 품종별로 검색한 결과, 세포독성의 유무는 1차 검색과 동일한 경향이 나타났으나, 벼에서는 품종에 따라 현저하게 세포독성의 차이를 나타내었으며, 특이할만한 점은 유색미인 흑도에서 다른 품종에 비하여 50% 세포성장저해농도가 유의하게 낮게 나타났다. Antineoplastic activities against human gastric and colon carcinoma cell lines were measured in seventeen methanol extracts of seventeen food and medicinal crops using the MTT(3-[4.5-dimethyl thiazol-2-yl)-2.5-diphenyl tetrazolium bromide) method. Three extracts(Hodeum vulgare, Schizandra chinensis and Zea mays)from seventeen tested crops have been reported to have antineoplastic effects. The extracts obtained from remaining fourteen tested crops failed to show significant cytotoxic effect at the concentration of less than $230{\mu}g/ml$(50% inhibitory concentration).

단풍취 분획물이 알콜대사효소에 미치는 영향

문형인,지옥표,문세훈,신말식 성균관대학교 약학연구소 1998 成均藥硏論文集 Vol.10 No.1

Spraque-Dawley계 수컷랫트에 계통분획한 단풍취의 각 분획물을 경구투여하고 혈청 ethanol농도와 간의 ADH활성에 미치는 효과를 검색 추적한 결과 알코올대사를 촉진시키는 성분은 주로 에탄올가용부에, 억제시키는 성분은 에탄올불용부에 주로 존재함을 추정할 수 있었고 현재 활성성분을 분리중에 있다.(1998년 9월 25일 접수, 1998년 10월 14일 수리) Effects of organic solvents fraction from Ainsliaea acerifolia ethanol extract on alcohol metabolism in rats were examined and the results were as follows: Ethanol souble fraction, after a single oral administration to rats, was found to cause a significant decrease in the serum ethanol concentration as well as enhancement of liver cytosolic alcohol dehydrogenase(ADH) activity, on the other hand, the fraction insouble in ethanol was found to cause an increase ethanol concentration in the blood and inhibit ADH activity.

백서에서 Depulpin^�과 Formocresol에 대한 치수와 치근단 조직의 반응

문형인,황인남,오원만 전남대학교 치의학연구소 2002 구강과학 Vol.14 No.2

One fifth dilution of formocresol is usually used for pulpotomy of the primary teach and emergency pulpotomy of the permanent teeth. However, the use of formaldehyde has been subjected to criticism because it may be absorbed into the blood stream and become distributed systemically, it may also alter the pulp tissue rendering it immunologically active, and have carcinogenic potential. Recently Depulpin^(r)(VoCo., Germany) gains popularity as a devitalizing agent during root canal therapy in spite of high concentration of 49% paraformaldehyde because it facilitate devitalization of pulp and make root canal therapy easier. But there have been not enough publications about the reaction of pulp and periapical tissue caused by Depulpin. This study was performed to evaluate the histological changes in pulp and periapical tissue of rats after pulpotomy using formocresol and Depulpin and to elucidate the toxic effects of these agents. Thirty six Sprague-Dawley rats were anesthetized by intraperitoneal injection of ketamine. Maxillary first molar teeth were used for pulpotomy with formocresol and Depulpin. Rate were sacrificed after 2days, 4days, 1week, 2weeks, 3weeks and 4weeks respectively. Specimens were histologically observed by light microscope changes in pulp and periapical tissue. The obtained results were as follows. 1. Formocresol group A zone of fixed tissue, in which odontoblasts could clearly be defined, was present directly underneath the pulpotomy dressing in almost all teeth of this group. This was followed by an area of necrotic tissue which resembled dried out fibrous tissue with no cellular detail except some pyknotic nuclei. In the specimens of after 2days, 4days, 1week, 2weeks in which vital tissue was present, it was separated from the fibrous area by a zone of inflammation. In the specimens of after 3weeks and after 4 weeks, inflammatory infiltrate was in the periodontal ligament adjacent to the apical foramina of the teeth. 2. Depulpin^(r) group The area of necrotic tissue which had no cells and fibers, was present adjacent to the dressing. This was followed by dried out fibrous tissue with no cellular details except some pyknotic nuclei. A short stump of vital pulp with odontoblasts was present at the end of the canal after 2days. Inflammatory infiltrate was in the periodontal ligament after 4days and after 1week. Severe root resorption and necrosis of periapical tissue opposite the root resorption site were defined after 2 weeks and after 3weeks. Periapical lesion which consist of necrotic tissue surrounded by fibrous connective wall, was found after 4weeks. The results indicated that Depulpin can cause more adverse reaction to the dental pulp and periapical tissue than formocresol, and further studies are needed for its clinical use with safety.

옥미수의 분획이 알코올 및 알콜대사효소에 미치는 효과

문형인,HyungInMoon 한국자원식물학회 1997 한국자원식물학회지 Vol.10 No.4

Spraque-Dawley계 응성흰쥐에 계통분획한 cornsilk의 각 분획물을 경구투여하고 혈청 Ethanol농도와 간의 Alcohol dehydrogenase활서에 미치는 효과를 검색 추적한 결과 알코올대사를 촉진시키는 성분은 주로 에탄올가용부에, 억제시키는 성분은 에탄올불용부에 주로 존재함을 추정할 수 있었다. Effect of various fraction from corn silk on alcohol metaholism in rats were examined and the results were as follows: ethanol souhle fraction. after a single oral administration to rats. was found to cause a significant deL'I'ease in the serum ethanol concentration as well as enh.lJ1cement of liver eytosolie ADH activity, on the other hand. the fraction imouhle in ethanol was found to cause an increase in the blood ethanol concentration and inhihit ADH activity.