固本祛瘀1號方의 抗癌活性에 關한 硏究(Ⅰ)

徐寅源,金聖勳,최봉균,金東熙 대전대학교 한의학연구소 2000 혜화의학회지 Vol.9 No.1

To evaluate the antitumor activity and antimetastatic effects of Gobongeerlhobang(GGIB), studies were done experimentally. The results were obtained as follows: 1.GGIB extracts exhibited a siginficant cytotoxicity against HT1080, A549, SK-MEL-2 and SK-OV-3 cell lines. 2.The T/C% was 120.7% in GGIB treated group in S-180 bearing ICR mice. 3.GGIB extracts exhibited inefficient adhesive effect of A549,B16-BL6 cell to complex extracellular matrix. 4.GGIB extracts showed a significant inhibition of lung metastasis of B16-BL6 cells in C57BL/6. 5.In CAM assays, angiogenesis was insignificantly inhibited in GGIB treated group than control group. These results suggested that GGIB extracts might be usefully applied for prevention and treatment of cancer.

Cd/Te 몰분율에 따라 MOVPE 법으로 성장된 HgCdTe 박막의 특성

서대원,Lanh, Ngoc-Tu,김창경,서상희,김병국,김진상 경북대학교 센서기술연구소 2002 센서技術學術大會論文集 Vol.13 No.1

약이성 음료(藥餌性飮料)에 관한 고찰

서연희,조후종,오성천 명지대학교 자연과학연구소 1996 자연과학논문집 Vol.14 No.-

우리나라 약이성 음료의 재료와 종류 및 효능의 다양성 등을 알아본 결과 약이성 음료의 종류에는 차혼성차류 4종, 꽃잎차 3종, 과일차 5종, 곡재차 3종, 약재차 7종외에 탕류 21종, 장류 7종 등이 있고, 이들을 효능별로 보면 간에 유효한 것이 17종, 소화기에 유효한 것이 25종, 호흡기에 유효한 것이 18종, 신경안정에 유효한 것이 13종, 그리고 양기에 효능이 있는 것이 3종 이었다. After Chosum periods, the culture of Suncha (green tea) was declined, but medicine beverage was developed with medicine foods. Among the traditional beverage which was seen to reports and treatises, Yusacha (tea analog with /without green tea), Tang (boiled herb extract), Jang(lactic acid fermented rice beverage), had distinguished variable medical effects. The effect of medicine beverage were related with protecting stomach, strengthening stomach, promoting digestion, robusting internal organs, and stablizing mind.

리팍시민이 흰쥐의 의액분비 및 생쥐의 소장수송능에 미치는 영향

서희경,태윤식,강시현,이현정,이현화,김승조,황성주 충남대학교 약학대학 의약품개발연구소 1994 藥學論文集 Vol.10 No.-

Rifaximin, the active ingredient in Normix??, has an extremely broad spectrum of action covering all intestinal germs and its absorption is practically zero. It is not appreciably absorbed through the gastrointestinal(GI) tract and not inactivated by gastric juice and is eliminated unchanged in stools. General pharmacology of rifaximin has not been studied sufficiently due to its negligible absorption. In this study, the effect of rifaximin on the gastric juice secretion was investigated in rats and its effect on the gastrointestinal motility was also investigated in mice. Rifaximin had no effect on pH, volume, acidity and pepsin activity of gastric juice in rats and also no effect on GI motility(charcoal transport) in mice. Therefore, rifaximin may have little effect on the GI tract.

정신과 폐쇄 병동 환경 평가에 영향을 미치는 임상변인 : 치료의 질적 향성을 위한 예비적 연구 The Preliminary Study for Promoting the Quality of Psychiatric Inpatient Treatment

서정석,류은정,이상미,한미희,최귀순,손인기,남범우 건국대학교 의과학연구소 2003 건국의과학학술지 Vol.13 No.-

Purpose: IS prelminary study was designed to assess the clinical variables influencing on the ward atmosphere in psychiatric closed ward and to promote the quality of psychiatric inpatient treatment. Method: 41 psychiatric inpatients were selected in Chung-ju hospital, Konkuk university. Ward Atmosphere Scale(W AS) was used to evaluate patients' perception of ward milieu. WAS score were compared among subgroups with respect to the clinical variables such as duration of admission, frequency of admission, and diagnosis. Results: Significantly, patients who have hospitalized for 1 month to 2 months reported lower score of WAS and patients who have hospitalized for longer than 2 months reported higher score of WAS. The relationship between educated levels and WAS score was not significant. First admitted patients reported significantly lower score of WAS. Bipolar patients reported significantly higher score of WAS and alcohol related patients reported significantly lower score of WAS. Conclusion: The 1st admitted patients, patients with alcohol problem and educated patients were had less satisfied with psychiatric closed ward. Thus, by considering these factors, more specified therapeutic approach and plan should be conducted.

대구지역 고등학생들의 흡연실태 및 니코틴 의존도

서영숙,윤희정,권진희,이성국 韓國學校保健學會 2003 韓國學校保健學會誌 Vol.16 No.1

The purpose of this study was to surveyed the smoking status and pattern, nicotine dependence and factors associated with smoking and nicotine dependence for high school students in Daegu. In March. 2000, 1,593 students from 7 male and 6 female high schools located in Taegu were assessed with self-completing questionnaire. The major findings were as follows; Percentage of male smokers was 15.0% in general high school, 30.9% in business high school and that of female smokers was 5.0% in whole woman's high school. The median score of FTQ was 4.0 among male smokers and female smokers. the mean of FTQ score was 4.07±1.96 in male and 3.94±1.63 in female. Percentage of students with high FTQ score(FTQ≥7) was 13.3% in male and 5.9% in female. In multiple logistic regression analysis, smoking had significant association with general characteristics such as personal-specifics factors, family environment factors and school environment factors. In multiple logistic regression analysis, nicotine dependence had significant association with school-age and duration of smoking. On consideration of above findings, to prevent students from smoking and to reduce the smoking rate, we have to try together in house, school and society.

신경회로망을 이용한 ATM에서 실시간 호 제어에 관한 연구

서동신,김희철,나상동 조선대학교 생산기술연구소 1996 生産技術硏究 Vol.18 No.2

A traffic control using the neuaral network (NN) algorithm is proposed for the ATM communication networks. In this paper, neuaral network algorithm is trained to estimate call admission rate from traffic load and link capacity, and link capacity assignment is optimized by advanced NN algorithm method which used learning rate and moment term. In the real-time call control, link capacity assignment of NN methed is compared to equivalent bandwidth(EB) method. Therefore. simulation results yield efficient ATM traffic control which is used neural networks training between quality of service and traffic parameter in the number of traffic type 1, 2. We Show that call reject rate by neural network algorithm using the NN methed is presented better than EB method in the training pattern.

미세알부민뇨를 동반한 인슐린 비의존성 당뇨병 환자에서 혈장 Endothelin의 변화

서예경,정상원,남직화,신병호,전종민,김정국,김동희,하승우,김보완 慶北大學校 醫科大學 1996 慶北醫大誌 Vol.37 No.3

목적 : 혈관내피세포에서 생성되는 endothelin(ET)는 강력한 혈관수축작용을 가진 펩타이드로 당뇨병 환자에서 혈관내피세포의 손상여부에 따라 혈중 농도가 증가할 수 있으며 혈관합병증과 관련이 있다는 보고가 있다. 그러나. 혈중 ET치의 상승과 당뇨병성 합병증의 상관성에 대해서는 논란이 많다. 이에 저자들은 인슐린 비의존성 당뇨병 환자에서 미세알부민뇨의 동반유무에 따른 혈중 ET치의 변화 및 혈장 ET치와 요중 알부민 배설률과의 상관성을 검토하였다. 대상 및 방법 : 인슐린 비의존성 당뇨병 환자중 미세알부민뇨를 보인 22명과 정상알부민뇨를 보인 43명을 대상으로 하였으며 혈장 ET를 측정하여 요중 알부민 배설률, 당뇨병성 망막증 및 당뇨병 관련인자들과의 관계를 비교하였다. 결과 : 당뇨병성 망막증이 합병된 군에서 망막증이 합병되지 않은 군에 비하여 혈장 ET치가 유의하게 높았던 반면 (각각 1.9±0.66, 1.5±0.76pg/㎖, p<0.05) 미세알부민뇨를 보인 군과 정상알부민뇨를 보인 군사이에 혈장 ET치는 통계학적으로 유의한 차이가 없었다(각각 1.9±1.14, 1.7±0.69pg/㎖). 또한 요중 알부민 배설률과 혈장 ET치 사이에 유의한 상관관계는 없었으며 미세알부민뇨를 동반한 인슐린 비의존성 당뇨병 환자군에서 혈장 ET치는 당뇨병의 관련인자들과도 유의한 상관관계가 없었다. 결론 : 인슐린 비의존 당뇨병 환자에서 혈장 ET치가 초기 당뇨병성 신증의 합병여부나 미세알부민뇨의 정도를 반영하기는 어려울 것임을 시사한다. Background : Endothelin(ET), a potent vasoconstritor peptide released from vascular endothelial cells, is known to be increased in diabetic patients with vascular damage and related with diabetic angiopathy. However, whether plasma ET levels may be related to diabetic complications is controversial. We studied the change of ET levels in non-insulin dependent diabetics with microalbuminuria and relation between ET and urinary albumin excretion rate. Methods : Plasma ET levels of 22 non-insulin dependent diabetics with microalbuminuria and 43 patients with normoalbuminuria were measured and relations of plasma ET to diabetic complications such as urinary albumin excretion rate, retinopathy and other associated factors were studied. Results : Plasma ET levels were significantly higher in patients with diabetic retinopathy than those without retinopathy(1.9 ± 0.66 vs 1.5 ± 0.76 pg/㎖. p<0.05). The difference, however, between diabetics with and without microalbuminuria was not significant(1.9 ± 1.14 vs 1.7 ± 0.69 pg/㎖). There were no correlations of plasma ET to urinary albumin excretion rate and to the other associated factors in non-insulin dependent diabetics. Conclusion : These results suggest that plasma ET levels may not seem to act as a predictor of diabetic nephropathy or microalbuminuria in non-insulin dependent diabetics.

구자가 卵巢摘出로 誘發된 흰쥐의 骨多孔症에 미치는 影響 (2)

徐富一,金先熙,卞晟僖,金美麗 대한본초학회 1998 大韓本草學會誌 Vol.13 No.2

This study was performed to investigate the effects of Allii Tuberosi Semen(菲子) on osteoporosis after ovariectomy in rats. Control group received a daily po administration of normal saline for 4 weeks after oophorectomy(OVX). The other experimental group received a daily po administration of water extract of Allii Tuberosi Semen for 4 weeks after oophorectomy(OVX-ATS). Therefore, we measured levels of calcium, phosphorus, ash weights in femur. These results were as follows : 1. In the level of bone phosphorus, the result of OVX was 63.85±1.86㎎/㎗, but the result of OVX-ATS was 68.18±0.85㎎/㎗. But there was no statistical significance. 2. In the level of bone calcium, the result of OVX was 141.66±2.19㎎/㎗, but the result of OVX-ATS group was 164.82±3.74㎎/㎗. And the result of OVX-ATS showed significant increase in comparison with OVX. 3. In the level of bone ash weight, the result of OVX was 0.31±0.005(g), but the result of OVX-ATS was 0.33±0.002(g). And the result of OVX-ATS showed significant increase in comparison with OVX. The observations from these studies and the past studies(=A study on the effects of Allii Tuberosi Semen in ovariectomized osteoporosis of rats) suggest possible protective effect of Alli Tuberosi Semen water extract against bone loss in ovariectomized rats.

식중독 세균 5속을 검출하기 위한 4개 주요 REP-PCR 반응성분의 최적화

서현아,박성희,송대식,김근성 한국산업식품공학회 2003 산업 식품공학 Vol.7 No.4

반복성 염기서열을 응용한 PCR법인 REP-PCR을 사용하여 식중독 미생물의 분리와 동정을 위한 연구에 적용하기 위하여 주요 식중독 유발세균 5속(genus), 6종의 균주를 사용하여 실험을 실시하였다. PCR 구성성분인 MgCl₂, dNTPs, REP sequence primer, 주형 DNA의 농도를 최적화하기 위한 연구 결과, MgCl₂의 농도가 2.0mN과 2.5mN일 때, 균주들의 fingerprinting pattern의 변화가 없거나 적은 것으로 관찰되어, 모든 속의 균주를 단일조건으로 분리?동정하기 위해서 MgCl₂의 농도는 2.5mN이 최적인 것으로 결론을 내렸다. dNTPs는 50μM의 농도를 적용하였을 때부터 전체 fingertprinting pattern범위의 주 단편들을 나타내는 균주들도 있었으나 총 단편의 수가 완벽하게 나타나지는 않았고, 200μM의 적용시점에서 6종의 균주 모두 단편의 수나 강도의 변화가 관찰되지 않았기 때문에 fingerprinting pattern의 파악을 위한 목적에는 200μM의 dNTPs 농도만으로도 충분하였다. REP primer는 적용한 농도가 증가함에 따라 단편의 수와 강도가 증가하였으며 Vibrio를 제외한 5종의 균주가 2.0μM의 primer를 적용했을 때 고유한 fingerprinting pattern을 나타냈다. 주형 DNA의 양을 변화시켜 적용하였을 때 DNA 양의 비율이 2배에서 5배까지 증가하여도 초기 미량 적용 시 생성되었던 fingerprinting pattern은 많은 차이를 보이지는 않았다. 그러나 적용되는 DNA 양에 따라 단편들의 강도와 수가 약간씩 점증하는 양상을 보였다. This experiment was conducted to optimize REP(repetitve extragenic palindromic sequence)-PCR reaction conditions in order to simultaneously differentiate the five different foodborne pathogenic bacterial genera of Escherichia, Salmonella, Shigella, Vibrio and Listeria. The four major PCR cocktail components of MgCl₂, dNTPs, primers and teplate DNA were considered and their optimum concentrations were determined using six reference strains. the optimized condentration of MgCl₂ was determined to be 2.5 mM in order to obtatin a consistent fingerprinting pattern. The similar fingerprinting pattern was obtatined when REP primers and dNTPs were added up to the concentration of 2μM and 200μM, respectively. As for template DNA, the numbers of PCR fragments were not mostly affected, but their intensities were slightly increased as the concentrations of the DNA were increased from 2 times to 5 times.