12M의 고해상도 360° 카메라를 사용한 주차장의 14면 주차 상태 판단 프로그램

이영지(Young-Ji Lee),이희열(Hee-Yeol Lee),고태영(Tae-Young Ko),곽동훈(Dong-Hoon Kwak),김재형(Jae-Hyung Kim),김주호(Joo-Ho Kim),오승진,이태윤(Tae-Yoon Lee),박상민(Sang-Min Park),이승호(Seung-Ho Lee) 대한전자공학회 2019 대한전자공학회 학술대회 Vol.2019 No.6

In this paper, we propose a program to identify 14 parking status in a parking lot using a high resolution 360° camera of 12M. The proposed program consists of three steps: Match with plane image, parking area detection algorithm, and discrimination of parking using learning method. Tests on a model car to evaluate the program to identify 14 parking status in a parking lot using a high-resolution 360° camera of 12M showed 100% accuracy for both parking and double parking. Therefore, the effectiveness of a program to identify 14 parking status in a parking lot using a high-resolution 360° camera of 12M proposed in this paper has been proved.

3,4‑Dichloroaniline promotes fatty liver in zebrafish larvae

Ji‑Seon Park,송정아,Jong‑Su Park,이상우,Jieon Lee,Han‑Jin Park,Woo‑Keun Kim,Seokjoo Yoon,Hang‑Suk Chun 대한독성 유전단백체 학회 2020 Molecular & cellular toxicology Vol.16 No.2

Backgrounds 3,4-Dichloroaniline (3,4-DCA) is a transformation product of herbicides that is commonly used as a reference in developmental toxicity studies (OECD TG 236) (Bonnet et al. in Environ Toxicol 22:78–91, 2007). However, the mechanisms underlying 3,4-DCA-induced hepatotoxicity are not well known. Methods We exposed zebrafish larvae at 72 hpf to 3,4-DCA for 3 days and observed lipid accumulation in liver treated with 10-μM 3,4-DCA using oil red O staining. Subsequently, we performed qRT-PCR analysis to determine the genes involved in the observed lipid accumulation. Results We found that genes related to lipogenesis (srebp1, pparγ, lipin1, and scd1) and ER stress (bip, atf4, ddit3, dnajc3, and edem1) were significantly upregulated. In addition, we found that ROS generation increased in the larvae treated with 10-μM 3,4-DCA. Moreover, glutathione-S-transferase activity in these larvae was increased by 3,4-DCA in a dose-dependent manner, and the expression of the inflammation marker il-1β increased. Conclusion Our results indicated that exposure to 3,4-DCA induced fatty liver in zebrafish larvae and that this, in association with additional factors such as ER stress response, can promote liver damage. We accordingly suggest that 3,4-DCA could be used to induce fatty liver in zebrafish larvae.

내시경적 조임근절개술후에 발생한 췌십이지장 가성동맥류 1예

박수영,김윤정,박진한,전현수,박시형,이영진,이은지,김지현 仁濟大學校 白病院 2010 仁濟醫學 Vol.31 No.-

We reported a case of pancreatic pseudoaneurysm, involving pancreaticoduodenal artery, developed after endoscopic retrograde cholangiopancreatography(ERCP) with endoscopic sphinterotomy. A 54 year old woman was transferred to our hospital due to sustained hematochezia and hypovolemia. A week before admission, she underwent ERCP with endoscopic sphinterotomy for acute cholangitis in local clinics. She developed abdominal pain and hematochezia at 12 hours after the procedures. A post procedure contrast enhanced computed tomography of abdomen demonstrated a pseudoaneurysm in inferior portion of pancreatic head. This was not present on the pre procedure contrast enhanced computed tomography of abdomen and was thought to arise from the pancreaticoduodenal artery as a complication of the pre sphincterotomy. She was brought to our emergency room in a state of hypovolemia. A upper endoscopy showed active bleeding from ampula of vater. An celiac angiography demonstrated pancreatic pseudoaneurysm involving inferior pancreaticoduodenal artery and selective coil embolization of was performed.

Production and Characterization of Enzymes Involved in Chitin Catabolic Cascade from a Bacterial Strain Isolated from Soil

( Ji Hyun Lee ),( Jeong In Lee ),( So Yeon Park ),( Yoo Kyeong Jang ),( You Bin Lim ),( Hye Yoon Lee ),( Young Min Jung ),( Hae Chang Lee ),( Jong Hwa Lee ),( Shaheen Amna ),( Jae Kweon Park ) 한국키틴키토산학회 2019 한국키틴키토산학회지 Vol.24 No.1

The present study was aimed to isolate the bacteria producing chitinolytic enzymes including chitin deacetylase (CDA) from soil origin. About 24 bacteria were screened for their chitinolytic enzymes producing ability on the basis of colloidal chitin. Amongst one of the most potent isolates designated to JI-02 was further selected as the most prominent chitinolytic and CDAlike enzyme (CDA-X) producer based on the ability of deacetylation of an artificial substrate pNP-acetamilide. The maximum production of CDA-X in crude enzyme from JI-02 was observed in the presence of 1% colloidal chitin at 37°C and pH 6.4 after 120 h of incubation. Potent enzyme activity of CDA-X was determined toward pNP-acetamilide, demonstrating that optimal pH, temperature and ionic strength of the CDA-X were assessed to be 7.0, 52°C and 125 mM, respectively. Unexpectedly, Nacetylglucosamine (GlcNAc) was not fully converted by the action of CDA-X to glucosamine (GlcN) under the established conditions. Further study on enzyme activity toward chitin-oligosaccharides consisting of multi-N-acetylglucosamine (GlcNAc)n, n=2-5 may be necessary to elucidate the mode of action which requires the minimum size of (GlcNAc)n. However, our data suggest that CDA-X can convert chitin to chitosan with the maximum yield of approximately 0.08g/L during the fermentation of the strain in the presence of chitin. Furthermore, we found that crude enzyme showed the significant chitosanolytic activity. Taken together, our data suggest that the strain JI-02 may be a potential candidate as a particular strain for better understating of chitin catabolic pathway.

RAW264.7 세포에서 interferon-r 및 LPS에 의해 유도되는 NO생성에 미치는 TALT-35의 영향

박종일,박경석,김종석,박지훈,윤은진,송경섭,서강식,김훈,윤완희,박승길,임규,황병두 충남대학교 생물공학연구소 2006 생물공학연구지 Vol.12 No.-

TALP-35 purified from human term placenta is known to increase microtubule polymerization and stabilize the polymerized microtubule. To examine the effect of TALP-35 on immune system this study was performed. MTT assay was performed to investigate the effect of TALP-35 on the proliferation of RAW264.7 cells. TALP-35 dose dependently suppress the proliferation of RAW264.7 cells at high concentration (above 1 μM) in unstimulated cells, in case of 10 μM TALP-35 treated cells the suppression was 25% but in stimulated cells it was only 15%. Cosedimentation assay was carried out to investigate whether TALP-35 can bind to tubulin of RAW264.7, monocyte/macrophage lineage of mouse, and polymerize it. TALP-35 polymerize the tubulin of RAW264.7 cells and sedimented in dose-dependent manner. To investigate the effect of TALP-35 on the expression of iNOS protein western blotting was performed. The expression level of iNOS was decreased dose dependently in high concentration of TALP-35 treatment. To examine the activity of iNOS, secreted NO was determined by method based on Griess reaction. Interferon-γ and LPS-stimulated production of NO from RAW264.7 cells was decreased dose dependently above 0.1 μM concentration of TALP-35 and 50% is decreased at 10μM of it. This study shows TALP-35 can control cytokine induced-iNOS expression therefore it might control inflammatory diseases.

축구선수의 Detraining과 Retraining이 혈청지질 및 호르몬농도에 미치는 영향

정정화,박재현,채종훈,성혜련,황지인,윤미숙,노금선,윤종관,윤영학,노순덕,정경숙,박일규,김은희,박현태,박상갑 대한스포츠의학회 1999 대한스포츠의학회지 Vol.17 No.1

The purpose of this study was to investigate the effects of detraining and retraining on serum lipid and hormones in soccer players. Subjects were seven male high-school soccer players. V˙O_2max was determined for each subjects by administering a treadmill test(initial speed: 90m/min, grade: 5%, increasing speed per 3 min: 30m/min). Serum lipid(T-C, TG, HDL-C LDL-C) and hormones(epinephrine, norepinephrine, growth hormones, cortisol) were assayed pre and post detraining in 10, 20, 30 days after retraining. The repeated ANOVA was used to determine significant differences. The 0.05 level of significance was as critical level for the study. The results of the study were as follows: 1. V˙O_2max(ml/min) were 3576.3±204.2ml/min pre detraining, 3234.1±198.9 ml/min post detraining. There are significant(p<.05) difference between pre and post detraining. In 10, 20, 30 days after retraining, V˙O_2max(ml/min) were 3601.4±170.9 ml/min. There were significantly(p<.05) increased in retraining periods. 2. V˙O_2max(ml/kg/min) were significantly(p<.05) decreased from 62.3±2.9 ml/kg/min to 55.9±4.7 ml/kg/min in detraining. In 10, 20, 30 days after retraining, V˙O_2max(ml/kg/min) were 62.4±3.4ml/kg/min, 62.7±2.3ml.kg/min, 67.3±7.2ml/kg/min respectively. There were significantly(p<.05) increased in retraining periods. 3. T-C were significantly (p<.05) increased from 166.6±8.5mg/dl to 175.3±10.3 mg/dl in detraining. In 10, 20, 30 days after retraining, T-C were 160.1± 3.2mg/dl, 156.7±3.7mg/dl, 140.3±9.0mg/dl. There were significantly(p<.05) decreased in retraining periods. 4. HDL-C were 61.4±6.6mg/di pre detraining, 5.3±6.6mg/dl post detraining. There are significant(p<.05) difference between pre and post detraining. In 10, 20, 30 days after retraining, HDL-C were 56.9±7.1mg/dl, 56.4±9.2mg/dl, 57.7±9.1mg/dl respectively. There were no significant difference in retraining periods. 5. The hormones(epinephrine. norepinephrine, growth hormone, cortisol) were changed as same patterns. Epinephrine were 26.0±7.0[g/ml pre detraining, 24.6±3.2pg/ml post detraining. In 10, 20, 30 days after retraining, epinephrine were 26.9±5.6pg/ml, 30.6±6.2pg/ml, 29.4±5.6pg/ml respectively. There were no significant difference in retraining periods. In conclusion, HDL-C, epinephrine, norepinephrine, growth hormone and cortisol were decreased, T-C, LDL-C and TG were increased in detraining. But HDL-C, epinephrine, norepinephrine, growth hormone and cortisol were increased, T-C, LDL-C and TG were decreased in retraining.

토양환경지도 자료를 이용한 콩밭 토양의 화학성 비교

박지숙,이민진,정재원,김미혜,이서연,지윤미,한준호,김유학,공명석,한광현,노희명 忠北大學校 農業科學硏究所 2015 農業科學硏究 Vol.31 No.2

It is important to interpret the soil chemical properties in order to effectively manage the farmland. This study was conducted to investigate the soil chemical properties of upland soybean field according to the soil series at 32 upland fields located in the parts of Gangwon-do. Soil sampling sites were selected by using the National Statistics DB and KSIS DB that is a soil environment information system provided by the Rural Development Administration. Soil samples were collected from 0~15 cm of top soil before seeding or transplanting, air-dried, passed through a 2 mm sieve, and analyzed for soil pH, EC, organic matter, available phosphorus (P2O5), exchangeable potassium, exchangeable calcium and exchangeable magnesium. The average chemical properties have exceeded the each nutrient optimum range level of RDA, except the organic matter in experimental soil 2015. And despite application of conventional fertilization, each soil series nutrient contents were change respectively. The results of this study suggest that conventional fertilization should be applied based on the analysis about the amount of accumulated nutrient contents in the soil.

상관논리 활동을 통한 중학생들의 상관논리 유형 변화

박종윤,김지영,남정희,이상권,최병순 한국과학교육학회 2002 한국과학교육학회지 Vol.22 No.4

본 연구에서는 우리나라 중학교 학생들의 상관논리형성정도와 그 유형을 알아보고, 상관논리 활동을 통하여 학생들의 상관논리에 어떤 변화가 있는가를 알아보고자 하였다. 이를 위하여 중학교 2학년 학생 111명을 실험집단으로, 55명을 통제집단으로 선정하였다. 사전 검사에서는 두 집단 모두 상관논리의 형성률이 낮았으며, 집단간 차이는 없었다. 사후 검사에서는 실험집단의 상관논리 형성률이 유의미하게 증가하였으며, 통제집단의 경우는 사전 검사와 차이가 없는 것으로 나타나 상관논리 활동이 상관논리 형성에 도움이 되는 것을 알 수 있었다. 그러나 사후 검사에서도 실험집단의 정답률이 객관식 문항에서는 27%, 서술형 문항에서는 6% 정도로 나타나 상관논리가 완전히 형성된 학생들의 비율은 상당히 낮은 것으로 나타났다. The main purpose of this study is to investigate whether the correlational reasoning activities could improve middle school students' correlational reasoning abilities. The subjects were 8th grade students, and the correlational reasoning activities of the CASE(cognitive acceleration through science education) program was intervened to the experimental group(m=111) during science class while usual science instruction was given to the control group(m-55). The correlational reasoning test was administered before and after the intervention. The pre-test results showed that most of subjects performed poorly in correlation problems and no significant differences were found between the two groups. In the post-test, the experimental group showed enhanced performance while the control group did not. Therefore it can be concluded that CASE program activities help the students to improve correlational reasoning ability. However, the proportions of students gave correct response were not so high: 27% for the multiple-choice item and about 6% for the essay-type items.

Water Dropper의 靜電氣特性

朴奉斗,尹志洪,韓聖愚 경북대학교 과학교육연구소 1978 科學敎育硏究誌 Vol.2 No.-

Quantitative analysis of the static electricity charged on the water dropper has been performed with an electrometer and recorder. Experiments were carried out over various parameters such as nozzle diameter, water pressure, different kinds of liquids and temperature with time. The accumulated charge on the cans of the water dropper was related to the size of nozzle outlet and hydrostatic pressure, but were independent of the temperature of liquids. There was no charge, of course, on the nonpolarized molecules such as C_6H_6, CCl_4, and kerosen fuel.

한국산 자리공 자리공 열매의 적색소를 이용한 식용색소 : Ⅱ. 활성 사포닌을 제거한 색소의 안정성과 가공적성 Ⅱ. Color Stability and Characteristics as Food Colorant of Saponin-Free Pokeberry Pigments

박용석,서지연,황혜정,박기환,윤광로 中央大學校 食糧資源硏究所 2003 食糧資源硏究所 論文集 Vol.15 No.1

우리나라산 자리공 열매의 색소 함량은 산지에 따라 건물 g당 48.56에서 62.16mg으로 천연색소 자원으로 높은 편이였으며 산지간의 격차가 크지 않아 자원성이 좋은 편이었ㄷ. 자리공 열매의 적색소를 absolute ethanol 침전법으로 분획하는 과정에서 활성 사포닌이 95% 이상 제거되며 이 색고 침전물을 silica gel column이나 Sephadex G-25 column으로 정제함으로서 색소의 순도를 높일 수 있었다. Absolute ethanol 처리와 column 정제법을 응용하여 활성 사포닌을 제거 한 실험용 자리공 열매색소 분획 모두 색소 안정성이나 가공적성에서 뚜렷한 차리를 보이지 않았다. 따라서 absolute ethanol만 처리한 색소제품이나 이를 column chromatography로 정제한 색소분획 모두 고순도의 천연색소 첨가물로의 응용 가능성은 분명하였다. 이들 활성 사포닌을 제거한 자리공 열매색소의 색소안정성과 가공적성은 다음과 같이 요약 할 수 있다. 즉, pH 안정범위는 4.8-6.0이었으며, 열에 대한 안정성을 조사한 결과는 4℃에서 45일 저장했을 때는 70%의 색소 잔존율을 나타낼 정도로 안정한 편이었지만 25℃에서는 10일째에서 70% 잔존율을, 50℃이상의 온도에서는 수 시간 정도에서 50%이하의 색소 잔존율을 보여 전반적으로 열 안정성은 불량한 편이었다. 한편 이들의 존재 하에서는 급격한 변색이 진행되었다. 자리공열매 색소에 대하여 ascorbic acid나 palmityl ascorbate의 첨가는 색소 잔존율에 큰 영향을 미치지 않았으며 chlorogenic acid, resocinol 그리고 catechin의 첨가도 마찬가지였다. 포도당, 과당, 맥아당, 설탕, 펙틴, 셀루로스 그리고 cyclodextrin 등의 탄수화물도 전혀 영양을 미치지 않았다. 1000ppm 이내의 EDTA나 citric acid, malic acid 또는 oxalic acid 등의 산도 미치지 않았다. 금속이온으로서, Ca^(2+)과 Al^(3+)의 첨가는 색소의 잔존율에 큰 영향을 미치지 않았으나, Cu^(2+)나 Fe^(2+)의 첨가는 심한 변색을 초래하였다. 자리공 열매즙의 무수 알코올 처리물(적색 색소 침전물)은 soft gel, firm gel 또는 캔디 등의 고형 식품에 잘 어울리고, 음료에서는 변색이 빠르게 진행되며, 특히 요구르트의 경우가 가장 민감하였다. 결론적으로 보면, 우리나라산 자리공 열매에서 활성 사포닌을 제거하고 확보한 색소제품은 사탕무의 betanin과 거의 같은 색소안정성과 가공적성을 보여 줌으로서 사탕무색소 첨가제인 beet red를 대신할 수 있으며 색소의 함량이나 순도라는 관점에서는 훨씬 우수할 것으로 사료된다. To evaluate the basic data on the color stabilities of saponin-free pokeberry pigment products, % remain of pigment were calculated from absorbances of solutions stored at various conditions. The pigment products were estimated to be very stable at pH 4.8-6.0. Thermostability of pH 5.0 solution of pigment products could be kept stable below 4℃. Addition of Cu2+ or/and Fe2+ decreased the % remain of pigment, whereas most food components had no offset the stability of pokeberry pigment. The color changes of some model foods having pokeberry pigment were checked by Hunter color differerence meter for 45 days. In solid foods like gels and candy the changes of color difference were very slower than that of model beverages. Application of pokeberry pigment to soft drink and yogurt were found to be effective at the refrigerated condition.