New Zealand White 토끼의 생식세포 및 체세포 분열에 의한 염색체 분석

신선희,김희수,최영현,이원호 부산대학교 유전공학연구소 2001 분자생물학 연구보 Vol.17 No.-

토끼(New Zealand White rabbit)의 감수분열 및 유사분령상을 통해 그 염색체적 특징을 조사하였다. 감수분열 염색체의 표본 작성은 공기건조법을 다소 변형하여 사용하였고, 유사분열 중기상의 핵형분석에는 G-분염법을 이용하여 다음과 같은 결과를 얻었다. 1. 제1감수분열의 접합기와 태사기의 염색소립과 sex vesicles 및 동원체가 뚜렷하게 구별되었다. 태사기의 중기에서부터 후기 과정이 진행되는 동안에 2가염색체에서 측면으로 돌출한 머리카락 모양의 돌기들이 관찰되어졌고, 이 돌기들은 염색체가 lampbrush 염색체 구조의 모양이 되도록 해주었다. 이동기의 염색체들은 키아즈마의 수와 위치에 따라 분류가 가능하였다. 제1 감수분열 전기의 후반부 과정과 중기Ⅰ 시기에는 2가염색체로 된 21개의 상염색체와 1개의 말단결합으로 연결된 X-Y 염색체를 관찰할 수 있었다. 2. New Zealand White rabbit의 생식세포에서 관찰된 2가 염색체의 형태는 1CH, 1TAl 및 2TA 2가염색체 유형이 대부분을 이루었다. New Zealand White rabbit에서의 평균 키아즈마 빈도는 약 30.2로 나타났으며, 키아즈마 빈도가 복사기, 이동기 및 중기Ⅰ로 분열이 진행됨에 따라 다소 감소하는 경향이 있었다. 3. New Zealand White rabbit의 핵형분석에 의하면 염색체 수는 44개(2n=44)로서, 8쌍의 중부염색체, 9쌍의 차중부염색체, 4쌍의 단부염색체로 된 21쌍의 상염색체와 중부염색체인 1개의 X 염색체 및 단부염색체인 1개의 Y 염색체로 구성되어 있음을 알 수 있었다. Chromosomal characteristics of New Zealand White rabbit was studied at meiosis and mitosis. The meiotic chromosomal preparations were made with the modified air-drying method and karyotype analysis was performed with the G-banding technique, using isolated mitotic metaphase chromosomes of the New Zealand White rabbit. Chromosomes, sex vesicles and centromeres could be could be classified in the zygotene and the pachytene of the meiosis Ⅰ. The hair-like processes projecting laterally from the axes of bivalent chromosomes at the mid-late pachytene were basis of the numbers and the locations of chiasma in the diakinesis. Twenty-one autosomal bivalents and a single unequal terminally associated X-Y bivalent were observed during the late prophase and the metaphase of the meiosis Ⅰ. Most of the bivalent types observed in the New Zealand White rabbit spermatocytes were 1CH, 1TAl, and 2TA bivalents. The mean chiasma frequency (CE) of the male New Zealand White rabbit was 30.2, and it was found that the CF value tended to decrease through the diakinesis and the metaphase Ⅰ. The karyotype of the New Zealand White rabbit was a male chromosome number of 44(2n=44), comprising 8 pairs of metacentric, 9 pairs of submetacentric, 4 pairs of acrocentric autosomes, metacentric X chromosome and acrocentric Y chromosome.

PET의 절연파괴와 공간전하효과

金永煜,신추연,김인수,윤성도 군산대학교 1993 群山水産專門大學 硏究報告 Vol.28 No.1

This paper examined the existance behavior of charged particles by measuring polarity inversion current Thermally Stimulate Current (TSC) and analyed appearance mechanism of polarity inversion current peak and also investigated relation between ionic space charge formation and dielectric breakdown by measuring D.C breakdown impulse breakdown D.C-impulse superposition as a sample of PET. As a result, we found that dielectric breakdown is likely to happen due to ionic space charge at the transient state when applied polarity inversion voltage and that charged partion of TSC peak at the high temperature was the same as that of polarity inversion current. Also there was no effect on ionic space charge about the dielectric breakdown in stationary state when applied D.C voltage.

肺高血壓症 合倂 心房中隔缺損症 : 開心術1例報告 Open Heart Surgery Under Hypothermic Hemodilution Perfusion with 5% Dextrose in Water

李寧均,金永洪,金相仁,申明秀 醫藥係社 1964 綜合 醫學 Vol.9 No.9

캔디다균이 분비하는 단백질 분해효소의 순수 분리와 특성 및 교차 항원성

이경호,신운섭,박주영,김수기,박윤선,고춘명 연세대학교 원주의과대학 1995 원주의대논문집 Vol.8 No.1

開放性動脈管 : 31例手術報告 Report of 31 Cases

徐景弼,申漢壽,沈台燮,洪彰義,朱東雲,李寧均 최신의학사 1967 最新醫學 Vol.10 No.5

Thirty one cases, 18 females and 13 males, were operated on for patent ductus arteriosus. There were three adult cases. Patent ductus arteriosus alone was present in 27, while in 4 there were significant associated cardiovascular anomalies. No deaths occurred in 24 patients isolated patent ductus a' eriosus without and with mild to moderate pulmonary hypertension. Severe pulmonary hypertension was present in 4 patients with an isolated patent ductus. An operative death occurred in the 2 patients in this group. One of them died as result of the tearing of the ductus during surgery. The remaining case died 3 days postoperatively., Patent ductus arteriosus with congenital cardiovascular anomalies was present in 4 patients. Single operative death occurred 7 days postoperatively because of bronchopneumonia of right lung and atelectasis of the left lung. There was an increased mortality when patent ductus arteriosus was associated with severe pulmonary hypertension. All survival patients showed very satisfactory postoperative results.

지속성 외래 복막투석 환자에서 염증 지표와 고해상도 B 모드 경동맥 초음파 소견의 연관성에 관한 연구

송현용,송영수,안철우,강신욱,최규헌,하성규,이현철,이호영,한대석 대한신장학회 2002 Kidney Research and Clinical Practice Vol.21 No.2

배 경 : 저알부민혈증 및 C-반응 단백(C-reactive protein, CRP)이 증가된 지속성 외래 복막투석 환자의 사망률이 높은 정확한 이유는 밝혀져 않다. 혈액투석 환자에서처럼 복막투석 환자에서 저알부민혈증 및 염증반응이 심혈관계 질환 위험인자 인지 알아보고자 하였다. 방 법: 비당뇨병성 말기 신부전, 지속성 외래 복막투석 환자 93명의 심혈관계 질환 위험인자들과 복막 투과 특성 및 알부민, CRP 등의 염증지표에 따라 고해상도 초음파를 이용한 경동맥내막-중간막 두께, 내막-중간막 면적, 플라크의 유무 등을 단면적으로 비교하였다. 결 과: 복막 투과 특성은 고투과막군 14명, 중간 고투과막군 38명, 중간 저투과막군 31명, 저투과막군 10명으로 각 군간의 경동맥 내막-중간막 두께, 플라크 유무 및 내막-중간막 면적의 차이는 없었다. 기존 관상동맥 질환 환자는 8예(8.6%)로 내막-중간막 두께는 관상동맥 질환이 없는 환자보다 증가되었고(0.79±0.21 vs 0.60±0.11 mm, p<0.05) 플라크 형성은 더 많이 보이나 통계적 유의성은 없었다(75.0 vs 63.5%, p>0.05). 혈청 알부민치와 경동맥 내막-중간막 면적은 역상관관계를 보였다(r=-0.27, p<0.05). 저알부민혈증 환자(혈청 알부민 <3.5 g/dL)는 정상 알부민혈증 환자보다 내막-중간막 두께가 증가되었고(0.67±0.15 vs 0.61±0.12 mm, p<0.05), 플라크도 유의하게 많았다(68.0 vs 55.8%, p<0.05). 혈청 CRP치와 경동맥 내막-중간막 면적은 양의 상관관계가 있었으며(r=0.21, p<0.05) CRP가 높은 환자(≥0.8 mg/dL, n=18)에서플라크가 많이 관찰되었으나(65.8 vs 50.0%, p<0.05) 경동맥 내막-중간막 두께 및 내막-중간막면적은 양군간에 차이가 없었다. 다변량 회귀 분석상 경동맥 내막-중간막 두께에 영향을 미치는 인자는 고령, 높은 CRP, 기존 심혈관계질환, 낮은 혈청 알부민 순이였다. 결 론 : 복막투석 환자에서 고해상도 초음파를 이용한 경동맥 내막-중간막 두께 측정은 심혈관계 질환 유무 예측의 비침습적 검사가 될 수 있고 저알부민혈증 및 높은 CRP과 같은 만성염증 지속상태가 복막투석 환자에서 죽상 동맥 경화증의 발생을 증가시킨다고 가정할 수 있다. Background : Continuous ambulatory peritoneal dialysis(CAPD) patients with low albumin(LA) and signs of inflammation reflected by increased C-reactive protein(CRP) level have an increased mortality, but the mechanism of this phenomenon is not clear yet. Methods : To answer whether LA and inflammation also enhance cardiovascular risk in CAPD patients, we performed cross sectional study measuring carotid artery intima-media thickness(IMT), calculated intima-media area(cIM area) and the presence of plaque by high-resolution B-mode ultrasonography in 93 non-diabetic CAPD patients. Results : Patients with coronary artery disease (CAD, n=8) had significantly increased IMT(0.79± 0.21 mm vs. 0.60±0.11 mm, p<0.05) and higher prevalence of carotid plaques(75.0% vs. 63.5%) compared to the non-CAD patients. Significant inverse correlation was observed between serum albumin (SA) level and cIM area(r=-0.27, p<0.05). Those patients with LA(SA <3.5 g/dL) had significantly increased IMT compared to non-LA patients(0.67±0.15 mm vs 0.61±0.12 mm, p<0.05). Prevalence of carotid plaques was also significantly higher in LA patients (68.0% vs. 55.8%, p<0.05). CRP level revealed a significant positive correlation with cIM area(r=0.21, p< 0.05). Patients with high CRP(≥0.8 mg/dL, n=18) had higher prevalence of carotid plaques (65.8% vs. 50.0%, p<0.05) compared to those patients with CRP <0.8 mg/dL, but IMT and cIMT area were not different. By multivariate logistic regression analysis, old age, high CRP, history of CAD and low SA were the independent risk factors affecting IMT. Conclusion : Our study strongly suggests that low albumin and chronic inflammatory state of CAPD patients could be associated with increasing atherosclerotic cardiovascular disease. (Korean J Nephrol 2002;21(2):285-294)

국내에서 유행하는 HIV의 전파 경로에 따른 Subtype 분포

이주실,남정구,김성순,강춘,최병선,김옥진,박미선,성봉모,서순덕,전수경,변승옥,신영오,조해월 대한감염학회 2001 감염 Vol.33 No.5

Background : Previous data have been reported that subtype B is prevalent in South Korea, but neither the extent nor the proportion of subtypes could be evaluated. This study was designed to analyze the distribution of HIV-1 subtypes, temporal instructions and transmission dynamics between epidemiological groups. Methods : 1,280 Koreans had been diagnosed as HIV seropositive during the period 1985 to 2000. Among them, 134 individuals were selected for this molecular epidemiological study. 134 DNAs were isolated from uncultured or cultured peripheral blood mononuclear cells. V3-V5 (0.7 kb) fragment of HIV-1 env gene was amplified by nested polymerase chain reaction and was sequenced. Results : HIV-1 isolates from thirty-seven homosexuals were all subtype B (100%). On the other hand, 66 isolates from 94 heterosexuals were subtype B (70%) and 28 were non B subtypes (30% : 13 A, 4 C, 2 D, 8 E , 1 G). Only subtype B strains were isolated from 73 males who were infected with HIV inside Korea while 16 B and 20 non B subtype strains were isolated from 36 males who were HIV infected outside of Korea. However, B and non B strains were isolated half and half from females who were infected inside Korea except one. Conclusion : The HIV-1 subtype B strains are prevalent in Korea from the early HIV infection until present in both homo and heterosexuals. Non B strains have been transmitted from men who were infected outside Korea to their spouses and casual partners. So, we need further study to monitor subtype B and non B HIV transmission in epidemiological groups of Korea, (Korean J Infect Dis 33:311∼318, 2001)

Arrayed waveguide collimators for integrating free-space optics on polymeric waveguide devices.

Shin, Jin-Soo,Lee, Chang-Hee,Shin, Sang-Yung,Huang, Guang-Hao,Chu, Woo-Sung,Oh, Min-Cheol,Noh, Young-Ouk,Lee, Hyung-Jong Optical Society of America 2014 Optics express Vol.22 No.20

<P>Array-type optical devices are important for wavelength-division multiplexing optical communication system to achieve small footprint, mass production, and reliability. For fabricating transmitter module in an array configuration, it is difficult to achieve a passive alignment of isolator, collimating lens, and laser diode. To facilitate array isolator integration, a waveguide collimator is proposed in this work by using a low-contrast, large-core polymer waveguide. The diffraction of a guided mode propagating through a free-space region is suppressed by enlarging the guided mode. The fiber coupling loss due to the enlarged mode was overcome by incorporating an adiabatic taper structure. The excess loss of waveguide collimator including the loss through a 400-μm free-propagation region was less than 1.0 dB.</P>

Tunable channel-drop filters consisting of polymeric Bragg reflectors and a mode sorting asymmetric X-junction.

Shin, Jin-Soo,Park, Tea-Hyun,Chu, Woo-Sung,Lee, Chang-Hee,Shin, Sang-Yung,Oh, Min-Cheol Optical Society of America 2015 Optics express Vol.23 No.13

<P>A tunable channel-drop filter as essential component for the wavelength-division-multiplexing optical communication system has been demonstrated, which is based on polymer waveguide Bragg reflectors. For an ordinary Bragg reflector, the filtered signal is reflected toward the input waveguide. Thus an external circulator is required to separate the filtered signal from the input port, though it increases the total footprint and cost. For this purpose, we employed dual Bragg reflectors and a mode sorting asymmetric X-junction. The Bragg reflector exhibited a maximum reflectivity of 94% for a 6-mm long grating, a 3-dB bandwidth of 0.39 nm and a 20-dB bandwidth of 2.6 nm. The mode sorting crosstalk in asymmetric X-junction was less than -20 dB, and linear wavelength tuning was achieved over 10 nm at the applied thermal power of 377 mW.</P>

PURIFICATION AND FUNCTIONAL AMINO ACID RESIDUES OF ACETOLACTATE SYNTHASE FROM PEA

Yung Soo Shin,Jae Soeb Lee . Jong Gun Chae,Jung Hyu Shin,Jung Do Choi 생화학분자생물학회 1996 생화학분자생물학회 춘계학술발표논문집 Vol.- No.-