RISS 학술연구정보서비스

검색
다국어 입력

http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.

변환된 중국어를 복사하여 사용하시면 됩니다.

예시)
  • 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
  • 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
닫기
    인기검색어 순위 펼치기

    RISS 인기검색어

      검색결과 좁혀 보기

      • 좁혀본 항목

      • 좁혀본 항목 보기순서

        • 원문유무
        • 음성지원유무
        • 원문제공처
        • 등재정보
        • 학술지명
        • 주제분류
        • 발행연도
        • 작성언어
        • 저자

      오늘 본 자료

      • 오늘 본 자료가 없습니다.
      더보기
      • 무료
      • 기관 내 무료
      • 유료
      • KCI등재후보

        Inhibition of pRB Phosphorylation and Induction of p21WAF1/CIP1 Occur During cAMP-induced Growth Arrest in Human Neuroblastoma Cells

        최영현,이상현 한국생명과학회 2003 생명과학회지 Vol.13 No.5

        To develop a new approach to the treatment of neuroblastoma cells we evaluated the effect of cAMP on the Ewing's sarcoma cell line CHP-100. We observed that the proliferation-inhibitory effect of cAMP analogs was due to cell cycle arrest and induction of apoptosis, which was confirmed by observing the morphological changes and DNA fragmentation. DNA flow cytometric analysis revealed that cAMP arrested the cell cycle progression at the G1 phase, which effects were associated with inhibition of phosphorylation of retinoblastoma protein (pRB) and enhanced binding of pRB and the transcription factor E2F-1. cAMP also suppressed the cyclin-dependent kinase (Cdk) 2 and cyclin E- associated kinase activity without changes of their expressions. Furthermore, cAMP induced the levels of Cdk inhibitor p21WAF1/CIP1 expression and p21 proteins induced by cAMP were associated with Cdk2. Overall, our results identify a combined mechanism involving the inhibition of pRB phosphorylation and induction of p21 as targets for cAMP, and this may explain some of its anti-cancer effects.Key words - cAMP, Apoptosis, G1 arrest, pRB, p21 신경아세포종에서 cAMP 처리에 의한 pRB의 인산화 억제 및 p21WAF1/CIP1의 유도최영현*·이상현1(*동의대학교 한의과대학 생화학교실, 1신라대학교 생명공학과)

      • KCI등재

        Inhibition of Cell Invasion by Indole-3-Carbinol in OVCAR-3 Human Ovarian Cancer Cells

        최영현,김성옥,Choi, Yung-Hyun,Kim, Sung-Ok Korean Society of Life Science 2011 생명과학회지 Vol.21 No.7

        본 연구에서는 식물체에 널리 분포하는 indole-3-carbinol (I3C)에 의한 OVCAR-3 인체 난소암세포의 이동성 및 침윤성 억제 가능성과 이와 연관된 기전을 조사하였다. 본 연구의 결과에 의하면 I3C에 의한 OVCAR-3 세포의 증식억제는 세포의 이동성 억제와 연관이 있었으며, 이를 wound healing 및 matrigel invasion assay로 확인 하였다. 아울러 I3C 처리에 의하여 transepithelial electrical resistance가 증가되었으며, cellular paracellular permeability는 감소되었는데, 이는 I3C 처리에 의해 세포 내 치밀결합(tight junctions, TJs)의 tightness가 증가되었음을 의미한다. RT-PCR 및 immunoblotting 결과에 의하면, I3C는 TJs의 구성 성분이면서 paracellular transport의 선택적 투과성을 조절하는 주요 인자인 claudin-3 및 -4의 발현을 유의적으로 억제하였다. 또한 matrix metalloproteinase (MMP)-2 및 -9의 활성이 I3C 처리에 의하여 매우 억제되었는데, 이는 그들의 mRNA 및 단백질 수준에서의 발현 감소와 연관성이 있었다. 따라서 I3C에 의한 OVCAR-3 난소암세포의 침윤성 억제는 TJs 기능의 강화와 MMP 활성의 저하가 주요 인자로 작용함을 알 수 있었다. In the present study, we investigated the effect of indole-3-carbinol (I3C), a natural compound present in vegetables, on the cell migration and invasion of OVCAR-3 ovarian cancer cells. Our results indicated that I3C inhibited the proliferation of OVCAR-3 cells, a process which was associated with inhibition of cell motility as determined by wound healing experiments and cell invasion studies. I3C treatment increased the tightness of the tight junctions (TJs), which was demonstrated by an increase in transepithelial electrical resistance and a decrease in paracellular permeability. The RT-PCR and immunoblotting results indicated that I3C repressed the levels of claudin-3 as well as claudin-4, proteins that comprise a major part of TJs and play a key role in the control and selectivity of paracellular transport. Furthermore, the activities of matrix metalloproteinase (MMP)-2 and MMP-9 were also decreased by treatment with I3C, which was connected with the down-regulation of their mRNAs and protein expression. The results suggest that I3C may be expected to inhibit cancer cell metastasis and invasion by restoring TJs and decreasing MMP activity in ovarian cancer cell line OVCAR-3.

      • KCI등재

        Linoleic Acid-Induced Growth Inhibition of Human Gastric Epithelial Adenocarcinoma AGS Cells is Associated with Down-Regulation of Prostaglandin E2 Synthesis and Telomerase Activity

        최영현 대한암예방학회 2014 Journal of cancer prevention Vol.19 No.1

        Background: Linoleic acid is the most abundant polyunsaturated fatty acid in human nutrition and found in most vegetable oils and certain food products. In the present study, we investigated the effects of linoleic acid on the growth of human epithelial adenocarcinoma AGS cells. Methods: MTT assay, flow cytometry, RT-PCR and Western-blot analyses were used to investigate the effects and underlying mechanisms of linoleic acid on AGS cells. The effects of this compound were also tested on prostaglandin E2 (PGE2) production and telomerase activity. Results: Our data indicated that growth inhibition of AGS cells by linoleic acid treatment was associated with induction of apoptosis. Linoleic acid treatment decreased the expression levels of the cyclooxygenase (COX)-2 mRNA and protein without causing significant changes in the COX-1 levels, which was correlated with the inhibition of PGE2 synthesis. Linoleic acid treatment also decreased the expression of human telomerase reverse transcriptase (hTERT), a main determinant of the telomerase enzymatic activity, and activity of telomerase, with inhibiting the expression of c-myc in a concentration-dependent manner. Conclusions: Taken together, our results indicate that linoleic acid inhibits the production of PGE2 and activity of telomerase by suppressing COX-2 and hTERT expression.

      • KCI등재

        科學敎育에서 科學-技術-社會 (Science-Technology-Society)的 接近의 動向과 課題

        최영현,이원호,홍순형 釜山大學校 師範大學 1993 교사교육연구 Vol.27 No.-

        과학교육에서의 새로운 운동인 STS(Science-Technology-Society) 프로그램이 과거 20년 동안 미국과 유럽의 각국에서 발달되어져 왔다. 이 프로그램의 주 목적은 지식, 과정, 창의성, 태도 및 응용의 영역에서 자유스런 토론을 통한 문제해결과 의사결정능력을 함양해주는 것이다. 본 연구에서는 STS 교육과정의 소개와 우리나라 과학교육과정에로의 적용에 관하여 논의하였다. 과학교육에서 STS 교육과정의 접근을 시도한다면, 무엇보다도 STS 프로그램의 적용 결과 나타난 문제점들을 면밀히 검토해 보아야 한다. 그리고 과학교육의 목적이 무엇인지에 대하여 의문점을 가져봐야 할 것이며, 현재 우리나라의 학제 구조상 종래의 과학교육 목적 위에 STS 교육과정의 내용을 보완하는 형태가 바람직할 것으로 생각된다. 아울러 새로운 교과내용의 연구와 개발에는 교사가 함께 참여 해야 할 것이다. Science-Technology-Society(STS) curricula, the new movement in science education, have been developed in America and Europe for the last 20 years. The major purpose of this program is to arise the ability of the problem solving and decision making through the democratic discussion in areas of knowledge, process, creativity, attitute and aplication. In present paper, we have introduced those STS curricula developed, and discussed about application to Korean science education. First of all, in order to introduce STS education to Korean science education, the problems related to STS curricula should be reviewed. And we must be questioned about the purpose of science education, be hopeful reorganize the educational systems since the begining of Korean science teaching methodology. In addition, teachers should be involved in the study and development of STS education approach for Korean science education.

      • KCI등재후보

        서비스디자인 방법론을 활용한 통합브랜드 전략개발 사례연구 : 국립아시아문화전당 통합브랜드전략개발을 중심으로

        최영현,김승인 한국디지털디자인학회 2012 디지털디자인학연구 Vol.12 No.3

        최근 학술자료와 연구보고서 및 고객중심의 서비스를 제공하고 있는 산업분야에서 혁신적인 서비스모델 결과물로 서비스디자인 방법론을 많이 활용하고 있음을 확인 할 수 있다. 서비스디자인 방법론의 관심은 다양한 분야에서 일어나고 있다. 이는 서비스디자인 방법론을 활용한 결과물들이 혁신적인 성과를 만들어 내고 있어서 이다. 서비스디자인 방법론에 관심을 가지는 이유를 살펴보면 고객 중심의 맥락적 상황을 세밀히 관찰하고 디자이너의 세밀한 관찰력과 통찰력을 통해 디자인적 사고로 혁신적인 서비스를 만들어내고 그 성과도 괄목할 만한 사례가 쏟아지고 있기 때문이다. 본 연구에서도 국립아시아문화전당의 통합브랜드 전략개발을 위해서 서비스디자인 방법론을 활용하여 고객관점의 잠재니즈 도출을 하였으며, 도출된 고객 잠재니즈는 통합브랜드 전략개발 시 고객가치에 기반 한 전략 수립에 활용했다. 본 연구는 공공서비스 분야의 통합브랜드 전략개발에 있어서 첫 시도이자 새로운 접근이라는 점에서 의의와 가치를 지닌다고 할 수 있다. The service design methodology has frequently been used as a product of the innovative service model in the industrial fields that have recently provided academic materials, research reports, and customer-centered services. Interest in the service design methodology is occurring in various fields, for products using the methodology have been achieving innovative outcomes. The reason for the interest consists in the fact that customer-centered context is examined in detail, and that an innovative service is created with design-oriented thinking through a designer's elaborate observation and insight, which have produced a number of notable results. The present study is of significance as a new attempt and approach that has used the service design methodology to develop a strategy for an integrated brand of the Asian Culture Complex.

      • KCI등재

        인체 유방암세포에서 TPA에 의해 유도된 matrix metalloproteinases 활성 및 침윤성 증대에 미치는 genistein의 영향

        최영현,김성옥,Choi, Yung-Hyun,Kim, Sung-Ok 한국생명과학회 2012 생명과학회지 Vol.22 No.7

        Genistein, a predominant isoflavone, has been shown to inhibit the growth of various cancer cells in vitro and in vivo without toxicity to normal cells. In the present study, we investigated the effects of genistein on the activity and the expression of matrix metalloproteinases (MMPs) in MCF-7 and MDA-MB-231 human breast adenocarcinoma cells. Our findings showed that MMP-9 and -2 activation was significantly increased in response to 12-O-tetradecanoyl phorbol-13-acetate (TPA). However, the increased activities of MMP-9 and -2 in TPA-treated cells were concentration-dependently inhibited by treatment with genistein, and this was also correlated with a decrease in the expression of their mRNA and proteins. In addition, a matrigel invasion assay showed that genistein reduced TPA-induced invasion of MCF-7 and MDA-MB-231 cells. Although further in vivo studies are needed, these results suggest that genistein treatment may inhibit tumor cell invasion and, therefore, act as a dietary source to decrease the risk of cancer metastasis. Genistein은 대두 및 그들의 부산물에 풍부하게 존재하는 isoflavone의 일종으로 정상세포에서는 독성을 나타내지 않는 범위에서 다양한 in vitro 및 in vivo 모델에서 암세포의 증식을 효과적으로 억제할 수 있는 천연물로 알려져 있다. 본 연구에서는 MCF-7 및MDA-MB-231 유방암세포에서 matrix metalloproteinases (MMPs)의 활성 및 발현과 침윤성에 미치는 genistein의 영향을 조사하였다. 본 연구의 결과에 의하면 genistein은 12-O-tetradecanoyl phorbol-13-acetate (TPA) 처리에 의하여 활성화된 MMP-2 및 -9의 활성을 유의적으로 차단하였으며, 이는 전사 및 번역 수준에서 MMP-2 및 -9의 발현 억제와 연관성이 있었다. 또한 matrigel invasion assay를 통하여 genistein은 두 유방암세포의 침윤성을 완벽하게 차단하였음을 관찰하였으며, 이러한 효과는 genistein의 세포독성 효과에 의한 것이 아니었음을 알 수 있었다. 비록 in vivo 동물 실험을 통한 부가적인 연구의 필요성이 있으나, 본 연구의 결과는 genistein이 암의 전이를 억제할 수 있는 효과적인 식이 소재임을 보여주는 것이다.

      • KCI등재후보

        인체 백혈병 세포에서 Histone Deacetylase Inhibitor Trichostatin A에 의한 세포주기 G1 Arrest 유발에 관한 연구

        최영현,우현주 대한암예방학회 2006 Journal of cancer prevention Vol.11 No.2

        Histone deacetylase (HDAC) inhibitors inhibit cell proliferation, induce differentiation and/or apoptotic cell death, and exhibit potent antimetastatic and antiangiogenic properties in cancer cells in vitro and in vivo. Although they are emerging as a promising new treatment strategy in malignancy, how they exert their effect on human leukemic cells is as yet unclear. The present study was undertaken to investigate the underlying mechanism of a HDAC inhibitor trichostatin A (TSA)-induced growth arrest and its effect on the cell cycle control gene products in a human leukemic cell line U937. TSA treatment induced the growth inhibition and morphological changes in a concentration-dependent manner. Treatment of U937 cells with TSA resulted in a concentration-dependent increased G1 cell population of the cell cycle as determined by flow cytometry. Moreover, 75 ng/ml TSA treatment significantly induced the population of apoptotic sub-G1 cells (10.9 fold of control). This anti-proliferative effect of TSA was accompanied by a inhibition of cyclins and proliferating cell nuclear antigen (PCNA), positive regulators of cell cycle progression, and cyclin-dependent kinases (Cdks) expression and concomitant induction of Cdk inhibitors such as p16, p21 and p27. Although further studies are needed, these findings provide important insights into the possible molecular mechanisms of the anti-cancer activity of TSA in human leukemic cells. (Cancer Prev Res 11, 114-122, 2006)

      • KCI등재후보

        SK-MEL-2 흑색종세포에서 Genistein에 의한 세포주기 G2/M Arrest 및 Apoptosis 유발

        최영현,박철,최우영,이정옥,홍수현,홍상훈,이원호,류충호,김정임 대한암예방학회 2006 Journal of cancer prevention Vol.11 No.1

        Genistein, a soy metabolite, is a potential chemopreventive agent against various types of cancer. There are several studies documenting molecular alterations leading to cell cycle arrest and induction of apoptosis in a variety of cancer cell lines; however, its mechanism of action and its molecular targets on human melanoma cells remain unclear. In this study, we monitored that genistein inhibited the cell growth in SK-MEL-2 human melanoma cells. It was found that genistein inhibits cell growth in a dose- dependent manner, which was associated with dendrite-like morphological change and apoptotic cell death. Flow cytometry analysis showed that genistein could cause an arrest at the G2/M phase of the cell cycle, which was associated with a down-regulation of cyclin-dependent kinase (Cdk2) and Cdc2 phosphorylation However, genistein did not affect the levels of Cdk inhibitor p21 (WAF1/CIP1) and tumor suppressor p53 in SK-MEL-2 cells. The induction of apoptotic cell death by genistein was associated with a down-regulation of anti-apoptotic Bid and an inhibition of apoptosis proteins (IAPs) family expression, and up-regulation of Fas and FasL protein. Genistein treatment also induced the proteolytic activation of caspase-3 and a concomitant inhibition and/or degradation of poly (ADP-ribose) polymerase (PARP), phospholipas C-γ1 (PLCγ1) and β-catenin. The present results suggest that genistein has divergent biological function, additional studies will be needed to evaluate the molecular mechanisms of the interaction between genistein and other agents. (Cancer Prev Res 11, 46-57, 2006)

      맨 위로 스크롤 이동