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Simple Sequence Repeat Primer에 의한 딸기의 Genomic DNA 분석
양덕춘,최성민,김무성,민병훈 경희대학교 생명자원과학연구원 2002 硏究論文集 Vol.22 No.-
본 연구는 진핵세포에 널리 산재되어있는 repeat sequence를 primer로 사용하여 딸기의repeat sequence 좌위를 증폭시켜서 분석하고 이를 같은 종일지라도 지역간의 차이로 인한 환경의 변화 또는 재배방법의 차이에 의해서 일어나는 변이를 분석하는 방법에 활용할 수 있도록 하기 위하여 수행하였다. GGC repeat sequence의 딸기 genomic DNA에 존재여부를 판명하기 위하여 (GGC)_(6) probe를 DIG을 사용하여 labeling하여 딸기 genomic DNA에 hybridization한 결과 확실한 spot이 나타났다. 또한 repeat sequence로 이루어진 UBC SSR primer 100개중에서 4가지 서로 다른 primer에서 선명한 band를 확인할 수 있어서 딸기의 genomic DNA내에 여러 종류의 repeat sequence가 있는 것으로 판단되었다. 따라서 각 품종별 표지로서 이용성을 알아보고자 PCR을 수행하였는 바 (ACC)_(6)와 (CCG)_(6)의 primer에서는 다른 band에 비해 매우 확실한 밴드를 형성하였다 이러한 PCR product의 염기서열분석을 위해 우선 대량증식을 위한 PCR의 적정 조건은 primer 10p㏖, DNA 50ng으로서 수행하는 것이 DNA의 증폭에 좋았다. 증폭된 1 Kb DNA 절편을 이용하여 염기서열을 조사하였던 바, 808bp의 서열을 알아냈고 그 반복염기서열은 효모와 사람에 존재하는 유전자이었으며 Cdontella sinensis와의 유의성 검정결과 ACC 반복염기에 62% 동일성을 나타내었다. This study was carried out to investigate genetic variation caused by environmental change or cultural method difference, which was performed by amplifying repeat sequence loci of strawberry widely dispersed in eukaryotic cell. DIG labeled(GGC)_(6), probe was used to test if GGC repeat sequence exists in strawberry genomic DNA, resulting in clear spot on the filter. Among the 100 UBC SSR primers which contain repeat sequence, four different primers amplified clear DNA fragments, suggesting that the repeat sequence exist in the genome of strawberry. PCR was also performed to test whether these primers could be used for a marker among strawberry cultivars. (ACC)_(6), (No. 861) and (CCG)_(6) (No. 865) primers showed very clear bands compared to the other primers. The amplified 808 bp of 1 kb DNA was sequenced, and database search using the sequence information showed that the repeat sequence was the gene existed both in yeast and human, and a significant sequence homology to Odontella sinensis was found.
Surface Modification and Fibrovascular Ingrowth of Porous Polyethylene Anophthalmic Implants
Yang, Hee-Seok,Park, Kwi-Deok,Son, Jun-Sik,Kim, Jae-Jin,Han, Dong-Keun,Park, Byung-Woo,Baek, Se-Hyun The Polymer Society of Korea 2007 Macromolecular Research Vol.15 No.3
The purpose of this study was to determine the effect of surface modification on the fibrovascular ingrowth into porous polyethylene (PE) spheres ($Medpor^{(R)}$), which are used as an anophthalmic socket implant material. To make the inert, hydrophobic PE surface hydrophilic, nonporous PE film and porous PE spheres were subjected to plasma treatment and in situ acrylic acid (AA) grafting followed by the immobilization of arginine-glycine-aspartic acid (RGD) peptide. The surface-modified PE was evaluated by performing surface analyses and tested for fibroblast adhesion and proliferation in vitro. In addition, the porous PE implants were inserted for up to 3 weeks in the abdominal area of rabbits and, after their retrieval, the level of fibrovascular ingrowth within the implants was assessed in vivo. As compared to the unmodified PE control, a significant increase in the hydrophilicity of both the AA-grafted (PE-g-PAA) and RGD-immobilized PE (PE-g-RGD) was observed by the measurement of the water contact angle. The cell adhesion at 72 h was most notable in the PE-g-RGD, followed by the PE-g-PAA and PE control. There was no significant difference between the two modified surfaces. When the cross-sectional area of tissue ingrowth in vivo was evaluated, the area of fibrovascularization was the largest with PE-g-RGD. The results of immunostaining of CD31, which is indicative of the degree of vascularization, showed that the RGD-immobilized surface could elicit more widespread fibrovascularization within the porous PE implants. This work demonstrates that the present surface modifications, viz. hydrophilic AA grafting and RGD peptide immobilization, can be very effective in inducing fibrovascular ingrowth into porous PE implants.
梁學道,朱進子,朴鍾修,黃寅壽,金政輝,金載洙,金熙悳,朱炳七 中央醫學社 1972 中央醫學 Vol.22 No.3
The biochemical reactions and the chemotherapeutic agents sensitivity test to the cholera El Tor vibrio strains isolated from the cholera epidemics in Pusan and Kyungnam province in 1969-1970 were studied and the following results were obtained. 1. The cholera red test to the isolated strains were all positive, but M-R and V-P tests were showed various patterns. 2. On the hemolysis test to the isolated 68 strains in 1969 epidemic were positive for only 2 strains (2. 9%), but the 80 strains isolated in 1970 epidemic were showed all negative results. 3. Hemagglutination test with slide glass method to the isolated strains were showed all positive results. The order of intensity of hemagglutination reactions were showed the guinea pig, rabbit, chicken, human, and sheep erythrocytes. 4. The isolated strains were growth on the range from 0% NaCl to 4% NaCl in alkaline 1% peptone water, but growth was inhibited in over 5% NaCl in alkaline 1% peptone water. 5. The isolated strains were growth on the range from pH 6. 0 to 10. 0 in 0.5% NaCl in 1% peptone water. 6. Sensitivity test of colistin containing 1000 units with paper disk (6. 5mm in diameter) method to the 388 isolated strains were showed the following results: 277 strains (71.3%) were resistant, and 14 strains (3.6%) were showed 10mm inhibition zone, 2 strains (0. 6%) were showed 15mm inhibition zone. 7. The result of sensitivity test to eight different chemotherapeutic agents were sensitive to chloramphenicol, erythromycin (100%), Bactrim (95. 0%), teracycline(90.0%) sulfadiazine
1970年度 釜山市 및 慶南地方에서 콜레라流行時 설사患者에서 分離한 V. parahaemolyticus 에 對한 硏究
梁學道,朱進宇,朴鍾修,黃仁壽,金政輝,金載洙,金熙悳,朱炳七 中央醫學社 1972 中央醫學 Vol.22 No.3
The authors made a study on the isolation of V. parahaemolyticus from diarrheal patients when cholera El Tor epidemic in Pusan and Kyungnam province in summer 1970. The authors made an attempt to bacteriological identification, Kanagawa phenomenon (hemolytic activity) on the modified N agatsuma blood agar plates, serotyping with slide agglutination and comparative sensitivity test of V. parahaemolyticus strains isolated to eight different chemotherapeutic agents including Trimethoprim/Sulfamethoxazole (Bactrim). The results were obtained as follows 1. Kanagawa phenomenon to 29 V. parahaemolyticus isolated from diarrhea] patients were all positive. 2. Serotypes were classified as pooling KI anti-serum (3 cases : K3???1 case, K4..?1 case, and K7???1 case), pooling KII anti-serum (15 cases : K9...1 case, K12??.7 cases, K12...2 cases, K13???2 cases, K15???3 cases), pooling KIII anti-serum (2 cases : K19..1 case, K23???1 case), pooling KIV anti-serum (3 cases : K2s??-2 cases, K28??.1 case), and pooling KV anti-serum (6 cases : K33... 3 cases, K38-3 cases). 3. Sensitivity test to colistin containing 1000 units with paper disk method to the 29 strains of V. parahaemolyticus were as follows 25 strains (84. 1%) were showed 16-19 mm, and 4 strains (15. 9%) were showed 12 mm of inhibition zone. 4. Sensitivity test to eight different chemotherapeutic agents with paper disk method to the 29 strains of V. parahaemolyticus were sensitive to Bactrim, tetracycline, and chloramphenicol (100%), but resistant to amphicillin, sulfadiazine, methicillin, and penicillin G (100%).
Pain Assessment in Brain Tumor Patients after Elective Craniotomy
( Young Deok Kim ),( Jae Hyun Park ),( Seung Ho Yang ),( Il Sup Kim ),( Jae Taek Hong ),( Jae Hoon Sung ),( Byung Chul Son ),( Sang Won Lee ) 대한뇌종양학회·대한신경종양학회·대한소아뇌종양학회 2013 Brain Tumor Research and Treatment Vol.1 No.1
Objective: This study was performed to assess the postoperative pain of brain tumor patients who underwent elective craniotomy and to evaluate the factors associated with pain intensity. Methods: From January 2010 to December 2011, 47 patients with newly diagnosed brain tumors who underwent craniotomy were enrolled. The postoperative pain status was assessed daily until discharge using the visual analogue scale (VAS). Results: The study participants comprised of 22 males and 25 females with ages ranging from 18-76 years (median age, 50 years). Patients were divided into two groups: the painful group included patients who had a VAS score of more than 3 during their hospital stay after the craniotomy, and the tolerable group included patients who had a VAS score of 1 to 3 during their hospital stay. There were no differences between the two groups in terms of age, sex, location of surgery, history of diabetes, hypertension and smoking, body mass index, and hospital stay. Univariate analysis revealed that operating time, length of wound, head fixation, and perioperative administration of opioid were not associated with the intensity of postoperative pain. Daily assessment of VAS revealed the two peaks of pain on the operation day and the 4th postoperative day. The intensity of pain during the ambulation period was higher than that during intensive care unit (ICU) stay. Conclusion: Pain following elective craniotomy for brain tumor removal is insufficiently managed, especially after discharge from the ICU. More attention needs to be paid to patients` pain throughout the hospital stay.
효과적으로 LED를 제어하기 위한 Windows기반의 DMX512 컨트롤 소프트웨어 구현
양병철(Byung-Chul Yang),지한결(Han-Kyul Ji),신현택(Hyun-Taek Shin),고광혁(Kwang-Hyuk Ko),신예호(Ye-Ho Shin),현득창(Deuk-Chang Hyun),이덕진(Deok-Jin Lee) 대한전기학회 2009 대한전기학회 학술대회 논문집 Vol.2009 No.7
DMX-512 protocol is a lighting protocol which can control the various lighting fixtures by linking as networks. Recently many lighting fixtures corresponding to DMX-512 have been manufactured. The software which makes LED Control of lighting fixtures easy and simple, is proposed in this paper. The complicated interface and production method were improved and each mode was divided for simple production and reducing confusion of operator. The producing effects were confirmed by simulation results.