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1971년 경남 함양군(咸陽郡)에서 유행한 설사환자에서 분리한 Salmonella Shigella 균의 세균학적 연구
양학도,김재수,주병칠,박수연,주진우 대한미생물학회 1972 大韓微生物學會誌 Vol.7 No.1
The authors made a study on the bacteriological identification about unidentified straina which were roughly screened by local health center, which an epidemic diarrhea was outbreak at Ham Yang Gun, Kyeongsang-'Namdo ia July 1971. And the authors made an attempt to bacteriological identification, serotyping with slide aggIatination, and determination of the susceptibility of identified strains to various chemotherapeutic agents. The results were obtained as foflows. 1. The isolated strains identified Shigella flexneri type 2b(2 strains) and SaimoaelIa paratyphi B(4 stralns). 2. Sensitivity test using with three concentrations chemotherapautic agents(Paper disk used; Eiken chemieal Co., Tokyo, Japan): (1) ShigelIa fIexneri type 2b isolated strains were sensitive to kanamycin, coIistin(100%) and penicillin (50%) respectively, but resistant to erythromycin, oleandomycin, leucomycin, chloramphenicoI, tetracycline, dihydrostreptomycin and sulfadimethoxin(100%) respectiveIy. (2) SalmonelIa paratyphi B isolated strains were sensitive to chloramphenicol, tetracyclihe, dihydrostreptomycin, kanamycin, sulfadimethoxine(l00%) and colistin(50%) respectively, but resistant to penicillin, erythromycin, oleandomycin and leucomycin(100%) respectively. 3. Sensitivity test using with multodisk urinary code(Paper disk used; Oxoid, London): (l) Shigella fIexneri type 2b isoIated strains were sensitive to Ractrim, ampicfllin, nitrofurantofn, nalidixic acid and gentamicin(100%) respectiveIy, but resistant to chIoramhpenicoI, tetracycline, sulfadiazine and FanasiI(100%) respectiveIy. (2) SaImonelIa paratyphi B isolated strains were sensitive to Bactrim, chloramphenicol, ampicillin, nitrofurantoin, tetracycline, nalidixic acid, gentamicin(100%) and sulfadiazine (50%) respectively, but resistant to Fanasil(100%) and sulfadiazine(50%) respectively.
梁學道,朱進子,朴鍾修,黃寅壽,金政輝,金載洙,金熙悳,朱炳七 中央醫學社 1972 中央醫學 Vol.22 No.3
The biochemical reactions and the chemotherapeutic agents sensitivity test to the cholera El Tor vibrio strains isolated from the cholera epidemics in Pusan and Kyungnam province in 1969-1970 were studied and the following results were obtained. 1. The cholera red test to the isolated strains were all positive, but M-R and V-P tests were showed various patterns. 2. On the hemolysis test to the isolated 68 strains in 1969 epidemic were positive for only 2 strains (2. 9%), but the 80 strains isolated in 1970 epidemic were showed all negative results. 3. Hemagglutination test with slide glass method to the isolated strains were showed all positive results. The order of intensity of hemagglutination reactions were showed the guinea pig, rabbit, chicken, human, and sheep erythrocytes. 4. The isolated strains were growth on the range from 0% NaCl to 4% NaCl in alkaline 1% peptone water, but growth was inhibited in over 5% NaCl in alkaline 1% peptone water. 5. The isolated strains were growth on the range from pH 6. 0 to 10. 0 in 0.5% NaCl in 1% peptone water. 6. Sensitivity test of colistin containing 1000 units with paper disk (6. 5mm in diameter) method to the 388 isolated strains were showed the following results: 277 strains (71.3%) were resistant, and 14 strains (3.6%) were showed 10mm inhibition zone, 2 strains (0. 6%) were showed 15mm inhibition zone. 7. The result of sensitivity test to eight different chemotherapeutic agents were sensitive to chloramphenicol, erythromycin (100%), Bactrim (95. 0%), teracycline(90.0%) sulfadiazine
1970年度 釜山市 및 慶南地方에서 콜레라流行時 설사患者에서 分離한 V. parahaemolyticus 에 對한 硏究
梁學道,朱進宇,朴鍾修,黃仁壽,金政輝,金載洙,金熙悳,朱炳七 中央醫學社 1972 中央醫學 Vol.22 No.3
The authors made a study on the isolation of V. parahaemolyticus from diarrheal patients when cholera El Tor epidemic in Pusan and Kyungnam province in summer 1970. The authors made an attempt to bacteriological identification, Kanagawa phenomenon (hemolytic activity) on the modified N agatsuma blood agar plates, serotyping with slide agglutination and comparative sensitivity test of V. parahaemolyticus strains isolated to eight different chemotherapeutic agents including Trimethoprim/Sulfamethoxazole (Bactrim). The results were obtained as follows 1. Kanagawa phenomenon to 29 V. parahaemolyticus isolated from diarrhea] patients were all positive. 2. Serotypes were classified as pooling KI anti-serum (3 cases : K3???1 case, K4..?1 case, and K7???1 case), pooling KII anti-serum (15 cases : K9...1 case, K12??.7 cases, K12...2 cases, K13???2 cases, K15???3 cases), pooling KIII anti-serum (2 cases : K19..1 case, K23???1 case), pooling KIV anti-serum (3 cases : K2s??-2 cases, K28??.1 case), and pooling KV anti-serum (6 cases : K33... 3 cases, K38-3 cases). 3. Sensitivity test to colistin containing 1000 units with paper disk method to the 29 strains of V. parahaemolyticus were as follows 25 strains (84. 1%) were showed 16-19 mm, and 4 strains (15. 9%) were showed 12 mm of inhibition zone. 4. Sensitivity test to eight different chemotherapeutic agents with paper disk method to the 29 strains of V. parahaemolyticus were sensitive to Bactrim, tetracycline, and chloramphenicol (100%), but resistant to amphicillin, sulfadiazine, methicillin, and penicillin G (100%).