Antiviral activity of Alpinia katsumadai extracts against rotaviruses

Kim, H.H.,Kwon, H.J.,Ryu, Y.B.,Chang, J.S.,Cho, K.O.,Hosmillo, M.D.T.,Rho, M.C.,Park, S.J.,Lee, W.S. British Veterinary Association [etc] ; W.B. Saunde 2012 Research in veterinary science Vol.92 No.2

In vitro anti-rotavirus activity of Alpinia katsumadai (AK) extracts were evaluated against bovine G8P[7] and porcine G5P[7] rotaviruses in two different assay strategies, a mixed treatment assay and a post treatment assay. In the mixed treatment assay, six AK extracts [AK-1 (EtOH extract), AK-3 (H<SUB>2</SUB>O layer), AK-5 (40% methanol fraction), and AK-9-11 (H<SUB>2</SUB>O extract, polysaccharide fraction, supernatant fraction)] exhibited inhibitory activities against G5P[7] rotavirus with the EC<SUB>50</SUB> values ranging from 0.7+/-0.4 to 33.7+/-6.5μg/mL. Extracts AK-1, AK-3, and AK-5 inhibited rotavirus infection against G8P[7] rotavirus, the with EC<SUB>50</SUB> values of 8.4+/-2.2μg/mL, 6.5+/-0.8μg/mL and 8.4+/-5.0μg/mL, respectively. By hemagglutination inhibition (HI) assay, six AK extracts completely inhibited viral adsorption onto human RBCs in both strains of rotaviruses at less than 11μg/mL. However, in the post treatment assay, there was no anti activity shown against both strains of rotaviruses. As a result, six AK extracts were attributed mainly to having a strong interaction with hemagglutinin protein on the outer surface of rotavirus, resulting to blockage of viral adsorption.

Valproate 장용성 제제의 용출률과 함량 균일성 평가

김명진,정양희,김재연,조영환,노환성 한국병원약사회 1999 병원약사회지 Vol.16 No.4

Valproate is an effective epileptic drug to control partial seizure and infantile spasm specially but the therapeutic range is narrow, so it is objective of TDM. The efficacy and safety of drug therapy depends on the bioavailability and the dissolution and content uniformity tests are very important procedures for the controlling of medication bioavailability. This study selected three enteric-coated valproate tablet that is administered to patients 2 or 3 times daily in Asan Medical Center and evaluated the bioavailability by the dissolution test and content uniformity test, attempted to estimate that the release of valproate in vivo is suitable for present drug therapy or not. The dissolution test was performed in the solution Ⅰ and the solution Ⅱ that was made by K.P. 7 for three hours and all sample were measured by HPLC. The results were as followed : (1) The mean dissolution amount in the solution I was higher in order of three different brands B (14.9%) > A (7.52%) > C (2.45%) (2) The mean dissolution amount in the solution Ⅱ was higher in order of three different brands B (114%) > A (102%) > C (22.2%). (3) The content uniformity was higher in order to B (117.6%) > A (110.3%) > C (85.8%). In conclusion, the dissolution of A and B in vivo seems to be suitable for present drug therapy but the release of C in vivo seems to be not.

BELLE TOF 검출기의 특성 연구

남지우,김동욱,배미영,박강순,김정현,노상률,최영일,김희종,장동욱,최소명,권영준,Hikita, S.,Yu, C.,Yoshimura, Y.,Kichimi, H. 성균관대학교 기초과학연구소 1998 論文集 Vol.49 No.-

We investigated the properties of BELLE Time Of Flight(TOF) counter using the data taken in Jan 98 beam test at Pi2 beam line at KEK 12 GeV proton synchrotron. Charge distributions from readout PMT were analysed in comparison with dE/dx distributions predicted by GEANT full simulation. Experimental setup of the beam test is described, then procedures of data analysis, Monte Carlo simulation and analysis results are also described.

Development of Bovine Embryos Produced by Intracytoplasmic Sperm Injection (ICSI)

Ock, S.A.,Kwack, D.O.,Cho, S.R.,Cho, S.K.,Yeao, E.H.,Yoo, J.G.,Lee, Y.R.,Lee, H.J.,Choe, S.Y.,Rho, G.J. 韓國受精卵移植學會 2002 한국동물생명공학회지 Vol.17 No.1

Intracytoplasmic Sperm Injection (ICSI) has been widely used fur both human infertility and basic research. However, the high incidence of chromosomal abnormality is severe problem in cattle. Various oocyte activation stimuli, therefore, were compared by assessment of developmental capacity and chromosome analysis. Motile sperm selected by Percoll-density gradient were treated with 5 mM dithiothreitol (DTT) and injected into an oocyte matured fur 24 h. Eggs were then allocated into 5 treatment groups. Group 1 (control), sperm injection was performed without any further activation stimuli to the oocytes. Group 2 (handled control), sham injection was performed without sperm. In Group 3, oocytes exposed to 5 (M ionomycin for 5 min at 39(C. Group 4. ionomycine + 1.9 mM demethylaminopurine (DMAP, 3 h) and Group 5, ionomycine + 3 h culture in Ml99 + DMAP. Cleavage and the later development rate in Groups 1, 2 and 3 were significantly (P<0.05) lower than those in Groups 4 and 5. The incidence of chromosomal abnormality in the embryos treated directly with DMAP after ionomycine was relatively higher than in the embryo of Group 3 h, delayed DMAP treatment. From this results DMAP caused to be arrested the release of the 2nd polar body, resulting in changes of chromosomal pattern. Therefore, the time interval between ionomycin and DMAP is a crucial role in bovine ICSI.

돼지고기를 단백질 급원으로 한 식이가 쥐의 체내 카드뮴 대사에 미치는 영향에 대한 연구

정용기,노정해,이남형,한찬규 한국축산학회 1997 한국축산학회지 Vol.39 No.5

This study was performed to investigate the effect of feeding pork in the place of casein on cadmium toxicity in rats. Thirty six male rats of Sprague-Dawley weighing 225.2?9.3g were randomly blocked into six groups according to body weights. Three levels of cadmium in drinking water(0, 25 and 50 ppm) had been fed for 5 weeks. Pork replaced dietary casein as a protein source. Weight gains in either cadmium-added or pork-fed group tended to be lower than those of the control group(Cd 0 ppm and casein). The cadmium addition and pork feedings affect significantly feed efficiency according to factor analysis results. Hematocrit was decreased with cadmium intake(p$lt;0.05). Cadmium accumulation in liver showed a tendency to diminish in the pork-fed group among the cadmium-exposed groups. Hepatic enzyme activity of glutatione peroxidase and superoxide dismutase showed no significant differences among the treatments. Urinary and fecal cadmium excretions increased with pork feeding. The results indicate that pork feeding as protein source in diet may alleviate the cadmium toxicity in rats.

품종별 돈육의 물리화학적 특성 비교

김영붕,노정해,Richardson, I.,Wood, J. 한국동물자원과학회 2000 한국축산학회지 Vol.42 No.2

The physicochemical properties of pork from 4 different pig breeds (Duroc, Large white, Berkshire and Tamworth) was compared. Duroc showed the lowest pH as 5.99 in pH_(45m) in loin part. There was a significant difference among the breeds (p $lt; 0.05). However, in a pH_(24h) there was no difference among breeds. Moisture contents showed the ranges of 73.57% - 75.35% in loin part and there was a significantly difference(p $lt; 0.05) among the breeds. Berkshire showed the highest water holding capacity(WHC) value (66.10%) in loin part and Large white (54.43%), Duroc (43.78%) and Tamworth (42.94%) followed. There was significant differences (p $lt; 0.05) among breeds at 43min after slaughter in loin and rump, too. However, WHC was showed no difference among the breeds at 24hrs after slaughter. In colour, there was significant differences(p $lt; 0.05) on colour lightness(L value). For the redness(a value) and yellowness(b value) among the breeds. In rump, there was no significant difference except Tamworth. Hardness was slightly different from outer breeds (p $lt; 0.05), however cohesiveness and chewiness were not different frrom others. So, The high temperature deboning method is recommanded to Berkshire breed for manufacturing meat products.

지방산 결합단백질에 관한 연구 동향

권영아,노숙령,김혜경,함영태 中央大學校 食糧資源硏究所 1996 食糧資源硏究所 論文集 Vol.8 No.1

ABSTRACT Long chain free fatty acid (FFA) are the main energy source for many mammalian cell, and FFA metabolism is dependent upon the flux of FFA to sites of intra-cellular processing. Fatty Acid Binding Proteins (FABPs) are a family of abundant cytosolic proteins of low M.W.14-15 kDa. Although their precise physilogical role remains hypothetical, it has been proposed as a FFA transfer protein and as a binding protein responsible for controlling intracellular FFA concentration. Furthermore, significant correlations have been observed between the tissue content of FABP and the rate of FFA uptake or utilization in several different manipulations such as diet and hypolipidemic drugs. However, to date there has been no report which examines the role of FABP in physiological manipulations such as fatty liver, diabetes, obesity, hypercholesterolemia. Since lipid metabolism is abnormal and the liver is greatly affected in these disease, it is possible that FABP may be abnormal in these livers. Therefore, the realtionships of liver FABP and the disease with lipid abnormalities, especially fatty liver induced by alcohol and high cholesterol diet are examined in our lab. Where FABP is believed to be involved in FFA binding and transfer, cellular FABP amount as well as its binding activity are affected. If we further find the way to increase the expression of FABP in liver, it could be one means of settle for these kinds of diseases from lipid abnormality.

Microsatellite 를 이용한 돼지 Yorkshire 품종의 일당중체량 연관 DNA Marker 개발

송영민,김철욱,조광근,박외선,권은정,이정규,백동훈,최진성,노정만,홍연희 한국동물자원과학회 2000 한국축산학회지 Vol.42 No.4

This study was conducted to develop DNA markers related with average daily gain (ADG) using 27 pigs. Animals were divided into two groups: low ADG group had 11 pigs with 1.5 SD and high ADG group was consisted of 16 pigs with +1.5 SD. Initially, we choose and tested 78 microsatellites which could contain potential DNA markers for ADG. Part of the microsatellites were amplified by the polymerase chain reaction (PCR) and then electrophoresed on a agarose gel followed by the allelic, genotypic and statistical analysis. According to the alleic frequency analysis, 21 DNA polymorphisms were detected from 17 microsatellites and appeared to be associated with ADG (P$lt;0.05). From the genotypic frequency analysis, 14 DNA polymorphisms were found from II microsatellites and were associated with ADG. All pigs in the low ADG group had a 135:144 hetero type for the SWR308 marker, whereas all pigs in the high ADG group had a 144:144 homo type. This and possibly other selected markers could be useful for the selecting young pigs with an excellent growth potential.

麻藥 및 向精神性 醫藥品의 標準品 製造에 關한 硏究(Ⅱ) : 韓國産大麻의 性分硏究 및 標準品 製造에 關한 硏究 Studies on the components and preparation of reference standard from Korean Cannabis

元道喜,姜信晶,崔燉雄,李秀庭,朴相勇,韓貞愛,盧榮洙 국립보건연구원 1992 국립보건원보 Vol.29 No.2

節電 가능한 電子式 螢光燈 Ballast의 回路 設計

金相彬,金榮鎬,李在周,魯承容 서울시립대학교 산업기술연구소 1994 산업기술연구소논문집 Vol.2 No.-

In this paper, electronic ballast for fluorescent lamp is studied which satisfies the requirement of high efficiency and high power factor and is adequate to be implemented into simple circuit. The active power factor enhancement circuit is designed to move up the power factor. As a frequency converter, zero voltage switching resonant converter (ZVS_RC) that has little switching loss is implemented, which is for increasing the efficiency. The experimental results with two FL40T12 fluorescent lamp show that the power factor of electronic ballast for fluorescent lamp is 99,7 percent and thet the efficiency of switching circuit is 82 percent.