Acetylation of Smc3 by Eco1 Is Required for S Phase Sister Chromatid Cohesion in Both Human and Yeast

Zhang, Jinglan,Shi, Xiaomin,Li, Yehua,Kim, Beom-Jun,Jia, Junling,Huang, Zhiwei,Yang, Tao,Fu, Xiaoyong,Jung, Sung Yun,Wang, Yi,Zhang, Pumin,Kim, Seong-Tae,Pan, Xuewen,Qin, Jun Elsevier 2008 Molecular cell Vol.31 No.1

<P><B>Summary</B></P><P>Sister chromatid cohesion is normally established in S phase in a process that depends on the cohesion establishment factor Eco1, a conserved acetyltransferase. However, due to the lack of known in vivo substrates, how Eco1 regulates cohesion is not understood. Here we report that yeast Eco1 and its human ortholog, ESCO1, both acetylate Smc3, a component of the cohesin complex that physically holds the sister chromatid together, at two conserved lysine residues. Mutating these lysine residues to a nonacetylatable form leads to increased loss of sister chromatid cohesion and genome instability in both yeast and human. In addition, we clarified that the acetyltransferase activity of Eco1 is essential for its function. Our study thus identified a molecular target for the acetyltransferase Eco1 and revealed that Smc3 acetylation is a conserved mechanism in regulating sister chromatid cohesion.</P>

The Impact of Carrier Transport Confinement on the Energy Transfer Between InGaN/GaN Quantum‐Well Nanorods and Colloidal Nanocrystals

Jiang, Bin,Zhang, Chunfeng,Wang, Xiaoyong,Park, Min Joo,Kwak, Joon Seop,Xu, Jian,Zhang, Huichao,Zhang, Jiayu,Xue, Fei,Xiao, Min WILEY‐VCH Verlag 2012 Advanced Functional Materials Vol.22 No.15

<P><B>Abstract</B></P><P>The energy transfer (ET) between InGaN/GaN multiple‐quantum‐well (MQW) nanorods (NRs) and semiconductor nanocrystals (NCs) for efficient color conversion is studied. An exceptional contribution of carrier transport confinement to the ET mechanisms is observed in the proximal side‐wall coupling system, which consists of InGaN/GaN NRs and CdSe NCs. Under relatively low or high excitation, the ET rate shows different carrier‐density dependence, resulting from different electron‐hole configurations, i.e., bound excitons and free carriers. In the localized exciton regime, the ET rate decreases when increasing temperature from 20 K to 200 K. However, in the free‐carrier regime, the ET rate varies insignificantly in the same temperature range. The temperature dependence in this NR‐NC coupling system is different from that in the previously studied planar MQW‐NC coupling system. It is suggested that the carrier transport confinement in NRs is a major factor for these divergences. The highly efficient ET with efficiency up to 80% shows a promising potential of using such NR‐NC coupled structures for ET‐pumped, NC‐based, light‐emitting devices.</P>

Mutations of ARX and non-syndromic intellectual disability in Chinese population

Yufei Wu,Huan Zhang,Xiaofen Liu,Zhangyan Shi,Hongling Li,Zhibin Wang,Xiaoyong Jie,Shao-Ping Huang,Fu-Chang Zhang,Junlin Li,Ke-Jin Zhang,Xiao-Cai Gao 한국유전학회 2019 Genes & Genomics Vol.41 No.1

Mutations of Aristaless-related homeobox (ARX) gene were looked as the third cause of non-syndromic intellectual disability (NSID), while the boundary between true disease-causing mutations and non-disease-causing variants within this gene remains elusive. To investigate the relationship between ARX mutations and NSID, a panel comprising six reported causal mutations of the ARX was detected in 369 sporadic NSID patients and 550 random participants in Chinese. Two mutations, c.428_451 dup and p.G286S, may be disease-causing mutations for NSID, while p.Q163R and p.P353L showed a great predictive value in female NSID diagnosis with significant associations (X2 = 19.60, p = 9.54e−6 for p.Q163R; X2 = 25.70, p = 4.00e−07 for p.P353L), carriers of these mutations had an increased risk of NSID of more than fourfold. Detection of this panel also predicted significant associations between genetic variants of the ARX gene and NSID (p = 3.73e−4). The present study emphasized the higher genetic burden of the ARX gene on NSID in the Chinese population, molecular analysis of this gene should be considered for patients presenting NSID of unknown etiology.

Magnetic alignment of MnBi crystals and magnetic properties of MnBi-Bi composites

Yongsheng Liu,Jincang Zhang,Zhongming Ren,Shixun Cao,Xiaoyong Zhang,Guangqiang Jia,Kang Deng,Xi Li,Junxi Zhang 한국물리학회 2007 Current Applied Physics Vol.7 No.5

Below Curie temperatureTC, MnBi crystals are aligned alongc-axis in a Bi matrix under a fabrication eldHfof 0.5 T. AboveTC,this alignment is also accomplished by quenching under a highHfof 10 T. Such a method has a prominent feature that MnBi crystalsgrow preferentially and congregate along theHfdirection. Magnetic testing shows a pronounced anisotropy in magnetization in direc-tions normal and parallel toHf, resulting from the alignment. In the case of the alignment belowTC,Hfincreases the transition temper-ature of spin-reorientation and the change in magnetization.

Hysteretic Behavior of Reinforced Concrete Columns Confined By Square Steel Tubes.

Xiaoyong Wang(왕소용),Sumei Zhang(장수메이),Lee, Han-Seoung(이한승) 한국콘크리트학회 2006 한국콘크리트학회 학술대회 논문집 Vol.2006 No.5

SORTING SEMICONDUCTING SINGLE-WALLED CARBON NANOTUBES AND USING THEM AS RANDOM NETWORK OF FIELD-EFFECT TRANSISTORS

LingLi Zhang,Weizhi Wang,XIAOYONG PAN 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2014 NANO Vol.9 No.4

Single-walled carbon nanotubes (SWCNTs) with incovalently attached iodine, were obtained byphysical absorption. The di®erent diameter sizes of SWCNTs, with di®erent numbers of iodinemolecule, enhance the density contrast between them which becomes evident in density gradientultracentrifugation (DGU) targeted to sort certain species of SWCNTs. The results of opticalabsorbance and photoluminescence emission showed that iodine-assisted DGU preferentiallyseparates semiconducting nanotubes with certain diameters [(6, 5), (7, 5), (8, 4), and (7, 6)].Wehave applied these semiconducting, species enriched SWCNTs to prepare solution-processed ¯elde®ect transistor (FET) devices with random nanotube network active channels. The devicesexhibit stable p-type semiconductor behavior in air with very promising characteristics. The on-o® current ratio reaches up to 2 ? 10 4 within a narrow window of voltage ( ? 10 V to 10 V), andestimated hole mobility of 21.7 cm2V? 1 s? 1 .

Validation of MCS code for shielding calculation using SINBAD

Feng XiaoYong,Zhang Peng,이현석,이덕중,이현철 한국원자력학회 2022 Nuclear Engineering and Technology Vol.54 No.9

The MCS code is a computer code developed by the Ulsan National Institute of Science and Technology (UNIST) for simulation and calculation of nuclear reactor systems based on the Monte Carlo method. The code is currently used to solve two main types of reactor physics problems, namely, criticality problems and radiation shielding problems. In this paper, the radiation shielding capability of the MCS code is validated by simulating some selected SINBAD (Shielding Integral Benchmark Archive and Database) experiments. The whole validation was performed in two ways. Firstly, the functionality and computational rationality of the MCS code was verified by comparing the simulation results with those of MCNP code. Secondly, the validity and computational accuracy of the MCS code was confirmed by comparing the simulation results with the experimental results of SINBAD. The simulation results of the MCS code are highly consistent with the those of the MCNP code, and they are within the 2s error bound of the experiment results. It shows that the calculation results of the MCS code are reliable when simulating the radiation shielding problems.

Identification of pepper genes involved in the response to CO 2 enrichment using RNA-Seq analysis

Jing Zhang,Rui Bai,Mengya Shang,Xiaoyong Xu,Hongxia Song,Shaowen Zheng,Leiping Hou,Meilan Li,Guoming Xing 한국원예학회 2021 Horticulture, Environment, and Biotechnology Vol.62 No.1

Pepper is widely cultivated, and the application of CO 2 promotes photosynthesis and increases its yield. However, the molecularmechanisms underlying this are still unclear. In this study, the photosynthetic correlation indexes under elevated CO 2and control conditions were compared. The application of CO 2 increased the photosynthetic capacity of pepper. Moreover,RNA-Seq analysis was used to identify genes that were diff erentially expressed between pepper leaves grown in CO 2 -enrichedconditions and those grown in control conditions. The 149 diff erentially expressed genes (DEGs) were found to be involvedin photosynthesis and other metabolic processes. According to GO signifi cant enrichment analysis, the proteins encodedby the DEGs were mainly found to be located in the chloroplast, the chloroplast matrix, and the apoplast. According toKEGG signifi cant enrichment analysis, the DEGs were found to be involved in glutathione metabolism; starch and sucrosemetabolism; and stilbenoid, diarylheptanoid, gingerol, fl avonoid, and phenylpropanoid biosynthesis. The DEGs were alsoinvolved in the pentose phosphate pathway, carbon metabolism, and porphyrin and chlorophyll metabolism. Based on theGO annotation and the KEGG database analysis, ten of the DEGs identifi ed were suggested to be involved in photosynthesisand related processes; these genes were predicted to have roles in carbohydrate, soluble sugar, and glutathione metabolism,and in raffi nose, cysteine, nucleotide, and ABA biosynthesis. These DEGs are involved in the pentose phosphate pathwayand tricarboxylic acid cycle of carbon assimilation during photosynthesis. One of the DEGs was also found to be involved inchlorophyll biosynthesis. These results lay the foundation for further investigation of the molecular mechanisms and genesinvolved in the response to CO 2 enrichment in peppers.

Time-optimal trajectory planning of serial manipulator based on adaptive cuckoo search algorithm

Lunhui Zhang,Yong Wang,Xiaoyong Zhao,Ping Zhao,Liangguo He 대한기계학회 2021 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.35 No.7

Feeding and unloading operation for part manufacturing are widely applied by industrial robots. In this paper, a set of algorithms has been used to reach higher efficiency and automation of trajectory planning for a 6-DOF integrated serial kinematic manipulator. Depending on the key nodes of the joint angles calculated by a hybrid inverse kinematics algorithm, the continuous quintic B-spline curve algorithm was utilized for planning smooth trajectories of the feeding motion from peer to peer. An adaptive cuckoo search (ACS) algorithm with high efficiency and excellent stability was proposed to minimize the total motion time under strict dynamic constraints. Comparing with 5 commonly used heuristic methods, the ACS algorithm has faster convergence speed and higher accuracy based on the same fitness function. To verify the implementation effect of the strategy, a 1:5 scale experimental platform was designed and built to implement the time-optimal trajectories. The simulations and experiments indicate that these algorithms lead to efficient planning of time-optimal and smooth trajectory in joint space.

Highly-sensitive Detection of Salvianolic Acid B using Alumina Microfibers-modified Electrode

Dong Sun,Xiaoyong Zheng,Xiafeng Xie,Xiaofeng Yang,Huajie Zhang 대한화학회 2013 Bulletin of the Korean Chemical Society Vol.34 No.11

Alumina microfibers with porous structures were prepared through hydrothermal reaction, and then used to modify the surface of carbon paste electrode (CPE). After modification with alumina microfibers, the electrochemical activity of CPE was found to be greatly improved. On the surface of alumina microfibersmodified CPE, the oxidation peak current of salvianolic acid B, a main bioactive compound in Danshen with anti-oxidative and anti-inflammatory effects, was remarkably increased compared with that on the bare CPE surface. The influences of pH value, amount of alumina microfibers and accumulation time were studied. Based on the strong signal amplification effects of alumina microfibers, a novel electrochemical method was developed for the detection of salvianolic acid B. The linear range was from 5 μg L−1 to 0.3 mg L−1, and the detection limit was 2 μg L−1 (2.78 nM) after 1-min accumulation. The new method was successfully used to detect salvianolic acid B in ShuangDan oral liquid samples, and the recovery was over the range from 97.4% to 102.9%.