miR-143 inhibits proliferation and induces apoptosis of mammary epithelial cells in dairy goat

Zhibin Ji,Guizhi Wang,Lei Hou,Zhaohua Liu,Jianmin Wang,Tianle Chao 한국통합생물학회 2016 Animal cells and systems Vol.20 No.2

MicroRNAs are a class of post-transcriptional regulators of gene expression in multicellular organisms, which play important roles in cell fate, organ morphogenesis and carcinogenesis. In the present study, we demonstrated the critical roles of miR-143 on mammary epithelial cells of dairy goat. The test results of cell count, methylthiazolyldiphenyl-tetrazolium bromide, Hoechst33342/PI (propidium iodide) and flow cytometry showed miR-143-induced G0/G1 phase arrest, inhibited proliferation and promoted apoptosis of mammary epithelial cells; the qRT-PCR test of marker genes related to cell proliferation and apoptosis, BAX and BCL-2, supported the same conclusions. Our study presents evidence that miR-143 is an important post-transcription regulator involving in mammary cells survival, and it may have a value function in mammary gland development, lactation or involution.

Alternating Copolymer of Sulfonated Poly(ether ether ketone-benzimidazole)s (SPEEK-BI) Bearing Acid and Base Moieties

Wang, Jianli,Song, Yilin,Zhang, Cheng,Ye, Zhibin,Liu, Hui,Lee, Myong-Hoon,Wang, Dehai,Ji, Jianbing WILEY-VCH Verlag 2008 Macromolecular Chemistry and Physics Vol.209 No.14

<P>A novel alternating copolymer of sulfonated poly(ether ether ketone-benzimidazole)s (SPEEK-BI) was synthesized by polycondensation of aromatic bisphenol containing benzimidazole and a mixture of sulfonated and non-sulfonated difluorobenzophenones. By varying the amount of two difluorobenzophenones, the ratio of sulfonic acid unit to imidazole base was controlled straightforwardly. The polymer structure was characterized by <SUP>1</SUP>H NMR and IR spectroscopy. Two different methods of ion exchange capacity measurement supported a strong inter- and intra-molecular interaction between acid and base units, which resulted in an improved mechanical strength as well as avoiding phase separation. Tough membranes with good mechanical stability in hot water (80 °C) and alcohol were obtained by solution casting, which is potential for applications in polymer electrolyte membrane fuel cells and membranes for separation of liquids or gases.</P><P> <img src='wiley_img/10221352-2008-209-14-MACP200700610-gra001.gif' alt='wiley_img/10221352-2008-209-14-MACP200700610-gra001'> </P>

Combinatorial patterns of histone acetylations and methylations in the human genome

Wang, Zhibin,Zang, Chongzhi,Rosenfeld, Jeffrey A,Schones, Dustin E,Barski, Artem,Cuddapah, Suresh,Cui, Kairong,Roh, Tae-Young,Peng, Weiqun,Zhang, Michael Q,Zhao, Keji Nature Publishing Group 2008 Nature genetics Vol.40 No.7

Histones are characterized by numerous posttranslational modifications that influence gene transcription. However, because of the lack of global distribution data in higher eukaryotic systems, the extent to which gene-specific combinatorial patterns of histone modifications exist remains to be determined. Here, we report the patterns derived from the analysis of 39 histone modifications in human CD4<SUP>+</SUP> T cells. Our data indicate that a large number of patterns are associated with promoters and enhancers. In particular, we identify a common modification module consisting of 17 modifications detected at 3,286 promoters. These modifications tend to colocalize in the genome and correlate with each other at an individual nucleosome level. Genes associated with this module tend to have higher expression, and addition of more modifications to this module is associated with further increased expression. Our data suggest that these histone modifications may act cooperatively to prepare chromatin for transcriptional activation.

A Truthful and Low-Overhead Routing Protocol for Ad Hoc Networks

Dongbin Wang,Xiaofeng Wang,Xiangzhan Yu,Kaicheng Qi,Zhibin Xia 보안공학연구지원센터 2013 International Journal of Future Generation Communi Vol.6 No.2

Identification and Function Prediction of Novel MicroRNAs in Laoshan Dairy Goats

Ji, Zhibin,Wang, Guizhi,Zhang, Chunlan,Xie, Zhijing,Liu, Zhaohua,Wang, Jianmin Asian Australasian Association of Animal Productio 2013 Animal Bioscience Vol.26 No.3

MicroRNAs are a class of endogenous small RNAs that play important roles in post-transcriptional gene regulation by directing degradation of mRNAs or facilitating repression of target gene translation. In this study, three small RNA cDNA libraries from the mammary gland tissues of Laoshan dairy goats (Capra hircus) were constructed and sequenced, individually. Through Solexa high-throughput sequencing and bioinformatics analysis, we obtained 50 presumptive novel miRNAs candidates, and 55,448 putative target genes were predicted. GO annotations and KEGG pathway analyses showed the majority of target genes were involved in various biological processes and metabolic pathways. Our results discovered more information about the regulation network between miRNAs and mRNAs and paved a foundation for the molecular genetics of mammary gland development in goats.

Secured Green Communication Scheme for Interference Alignment Based Networks

Zhibin Xie,Xinquan Geng,Yunfei Chen,Kening Song,Benjamin Panful,Yajun Wang,Yinjie Su,Zhenkai Zhang,Ying Hu 한국통신학회 2020 Journal of communications and networks Vol.22 No.1

In this paper, a new security and green communicationscheme is proposed to the interference-alignment (IA) based networks. To achieve a secured communication, full-duplex receiversare utilized to transmit artificial noise (AN). Both the signals andthe ANs are used to harvest energy to realize green communication. For these reasons, the feasible conditions of this scheme areanalyzed first. Secondly, the average transmission rate, the secrecyperformance and the harvested energy are investigated. Thirdly,an optimization scheme of simultaneous wireless information andpower transfer (SWIPT) is given to optimize the information transmissionand the energy harvesting efficiency. Meanwhile, an improvedIA iteration algorithm is designed to eliminate both the ANand the interference. Furthermore, relay cooperation is consideredand its system performance is analyzed. The simulations show thatthe target average transmission rate is not affected by AN, whilethe secrecy performance can be greatly improved. The energy harvestingefficiency is also better than the traditional schemes. Asexpected, the average transmission rate further is improved withthe relay cooperation.

Epidemiology, Characteristics, and Prognostic Factors of Primary Atypical Teratoid/Rhabdoid Tumors in the Spinal Canal: A Systematic Review

Zhibin Li,Yubo Wang,Liyan Zhao,Yunqian Li 대한척추신경외과학회 2024 Neurospine Vol.21 No.1

Primary atypical teratoid/rhabdoid tumors (AT/RTs) in the spinal canal are rare central nervous system (CNS) neoplasms that are challenging to diagnose and treat. To date, there has been no standard treatment regimen for these challenging malignant tumors. Thus, we conducted this research to explore potential prognostic factors and feasible treatment modalities for improving the prognosis of these tumors. Articles were retrieved from the PubMed, MEDLINE, and Embase databases, using the keywords “atypical teratoid/rhabdoid tumor,” “rhabdoid tumor,” “spine,” “spinal,” “spinal neoplasm”, and “spinal cord neoplasm.” All eligible cases demonstrated SMARCB1-deficient expression validated by pathological examination. We collected and analyzed data related to clinical presentation, radiological features, pathological characteristics, treatment modalities and prognosis via Kaplan-Meier and Cox regression analyses. Thirty-six articles comprising 58 spinal AT/RT patients were included in the study. The median progression-free survival (PFS) and overall survival (OS) were 18 and 22 months, respectively. Kaplan-Meier analysis demonstrated significant survival improvements for OS in the nonmetastasis, male, radiotherapy and intrathecal chemotherapy groups as well as for PFS in the chemotherapy and radiotherapy groups. Multivariate analysis revealed that chemotherapy and radiotherapy were prognostic factors for improved PFS, and that intrathecal chemotherapy reduced the risk of mortality. Spinal AT/RTs are uncommon malignant entities with a dismal survival rate. Although our review is limited by variability between cases, there is some evidence revealing potential risk factors and the importance of systematic chemotherapy, intrathecal chemotherapy and radiotherapy in spinal AT/RT treatment modalities.

Preparation of Modified Nano-SiO₂ Oil Dispersion System and the Stability of Its O/W Emulsion

Zhibin Zhou,Jiexiang Wang 한국고분자학회 2016 폴리머 Vol.40 No.2

Nano-SiO₂ was organically modified with silane coupling agent-KH570 to prepare the modified Nano-SiO2 oil dispersion system. By increasing the dosage of KH570, the ζ-potential (absolute value) and the char yield values decreased gradually. When KH570 was 5%, the maximum contact angle with water reached 79.8°. Then, the modified Nano-SiO₂ was composed with petroleum sulfonate (PS) and its ability of reducing the O/W interfacial tension was measured. The results showed that, O/W interfacial tension reduced to about 5.86×10<SUP>-3</SUP> mN/m when the Nano-SiO₂ and petroleum sulfonate composite was mixed with the mass fraction of 0.2:0.3%. The emulsion stability coefficient was 2.15 after 120 min which was the minimum. It indicated that this compounded system had the best emulsifying ability because Nano-SiO₂ particles were dragged onto the O/W interface by the PS molecules to improve the intensity of O/W interface.

Function and Structure Design for Regional Logistics Information Platform

Wang Yaowu,Lu Zhibin 보안공학연구지원센터 2015 International Journal of Future Generation Communi Vol.8 No.4

Regional Logistics Information Platform is the core and key development factor of modern logistics industry. Through the construction of logistics information platform, it can greatly promote the information-oriented for modern logistics development. The concept, characteristic and requirements of regional logistics information platform are defined. The function and operation mechanism of it have been analyzed. Based on the analysis, the frame of the information platform has been designed.

Sound Recognition of Harmful Bird Species Related to Power Grid Faults Based on VGGish Transfer Learning

Qiu Zhibin,Wang Haixiang,Liao Caibo,Lu Zuwen,Kuang Yanjun 대한전기학회 2023 Journal of Electrical Engineering & Technology Vol.18 No.3

Bird activities threaten the safe operation of transmission lines and substations. In order to assist differentiated prevention of bird-related faults in power grid, this paper proposes a birdsong recognition method based on VGGish transfer learning. Firstly, according to the information of bird species related to historical power grid faults and the investigation results of bird species around transmission lines, 18 high-risk, 18 low-risk, and 2 harmless bird species were selected to establish a sample set with their song signals. Then, the birdsong signals were preprocessed by framing, windowing, noise reduction and clipping, thus to extract the spectrogram, which was mapped to a 64-order Mel filter banks to get the Mel spectrogram. Aiming at weak generalization ability of traditional birdsong recognition models due to insufficient number of samples, the VGGish transfer learning network pretrained by AudioSet was used as the birdsong feature extractor, and the Mel spectrograms of harmful bird species belong to the training set were taken as inputs to train the network parameters, thus to extract 128-dimensional VGGish deep features for bird recognition. This method was applied to classify 38 kinds of bird species related to power grid faults, and the recognition accuracy reaches 94.43%. The research results can provide references for power grid inspector to carry out intelligent recognition and ecological prevention of bird species.