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      • KCI등재

        Combined with finite element analysis of car seat safety performance improvement

        Li Hongling,Dong Yude,Chen Changliang,Chen Chao 대한기계학회 2021 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.35 No.1

        Neck injuries caused by low-speed rear-end collisions are a worldwide problem. The reasonable design of car seat can effectively prevent or reduce the passenger neck injuries. In this paper, the finite element analysis (FEA) and whiplash test are combined to optimize the seat structure parameters to enhance the safety. The whiplash test results of the existing car seats according to C-NCAP (2015) were matched with FEA, and the optimization scheme was developed by computer simulation. For the improvement of seat safety, the optimizations are applied to determine backrest stiffness, backrest rotational stiffness, headrest rotational stiffness and headrest stiffness. The retest results of optimized seat show that its safety performance is greatly improved. Furthermore, it shows that the combination of finite element analysis and whiplash test can optimize seat structure to improve safety performance.

      • KCI등재

        Hydrogenation of methyl methacrylate under mild conditions using biosynthesis Ru catalyst

        Hongling Zhou,Yangqiang Huang,Youwei Cheng,Lijun Wang,Xi Li 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.47 No.-

        Hydrogenation of methyl methacrylate (MMA) was investigated over Ru-based catalyst supported onactive carbons (AC), which was prepared by bio-reduction method, using C. Platycladi (CP) leaf extract asreductant. By varying reaction temperature, hydrogen pressure and reaction time, hydrogenation ofMMA over the biosynthesis Ru-based catalyst was carried out, and resulted the optimum conditions,30 C (room temperature), 2.0 MPa, and 50 min. The as-prepared Ru/AC catalyst showed high catalyticactivity towards the hydrogenation of MMA, affording 100% of MMA conversion even under mildconditions without solvent and methyl isobutyrate was the only product.

      • KCI등재

        Genome-wide analysis of Gro/Tup1 family corepressors and their responses to hormones and abiotic stresses in maize

        Hongyou Li,Kaifeng Huang,Hanmei Du,Hongling Wang,Xin Chen,Shibin Gao,Hailan Liu,Moju Cao,Yanli Lu,Tingzhao Rong,Su-Zhi Zhang 한국식물학회 2016 Journal of Plant Biology Vol.59 No.6

        Gro/Tup1 proteins act as negative transcriptional regulators and play crucial roles in many growth and developmental processes in a wide range of organisms. However, our understanding of Gro/Tup1 protein functions in plants is confined to the model plant Arabidopsis. Here, 11 Gro/Tup1 genes, which were characterized by the typical LisH and WD40 repeat domains, were identified in maize through a genome-wide survey. A phylogenetic analysis revealed that maize Gro/Tup1 proteins could be divided into three subfamilies, in which members shared similar protein and gene structures. The predicted maize Gro/Tup1 genes were distributed on seven chromosomes and segmental duplication contributed to their expansion. Many predicted cis-elements associated with hormones, biotic- or abioticstress responses, meristem and seed development, and circadian rhythms, were found in their putative promoter regions. A potential associated protein analysis identified a large number of candidates, including transcription factors, chromatin-modifying enzymes, protein kinases, and ubiquitinconjugating enzymes. An expression profile derived from the RNA-seq data indicated that Gro/Tup1 genes in maize were widely expressed in various organs and tissues. Quantitative real-time PCR revealed that these genes responded to at least one hormone or abiotic stress, either in roots or in shoots. Our study provides useful information on the Gro/Tup1 genes in maize and will facilitate the further functional validation of these genes in growth and development, hormone responses, and biotic- or abiotic-stress resistance.

      • A revised Hermite peak factor model for non-Gaussian wind pressures on high-rise buildings and comparison of methods

        Dongmei Huang,Hongling Xie,Qiu-Sheng Li 한국풍공학회 2023 Wind and Structures, An International Journal (WAS Vol.36 No.1

        To better estimate the non-Gaussian extreme wind pressures for high-rise buildings, a data-driven revised Hermitetype peak factor estimation model is proposed in this papar. Subsequently, a comparative study on three types of methods, such as Hermite-type models, short-time estimate Gumbel method (STE), and new translated-peak-process method (TPP) is carried out. The investigations show that the proposed Hermite-type peak factor has better accuracy and applicability than the other Hermite-type models, and its absolute accuracy is slightly inferior to the STE and new TPP methods for non-Gaussian wind pressures by comparing with the observed values. Moreover, these methods generally overestimate the Gaussian wind pressures especially the STE.

      • Harris Scale Invariant Corner Detection Algorithm Based on the Significant Region

        Wu Peng,Xu Hongling,Li Wenlin,Song Wenlong 보안공학연구지원센터 2016 International Journal of Signal Processing, Image Vol.9 No.3

        The traditional Harris corner detection algorithm is sensitive to scale change, corners detected throughout the entire image under complex background, thus extracting more false corners, lead to the follow-up of large amount of calculation and a high rate of error matching. To solve this problem, this paper proposes an optimized Harris corner detection algorithm. First, a significant region detection method is used to extract the target area, and take closing operation for the result figure, can effectively achieve target and background segmentation; second, scale invariant describing methods is applied to Harris algorithm, at the same time, combined with the non-maximum suppression methods to extract corners, get more right corners. Through experiment contrasts, the algorithm used in this paper can be improved more corner detection performance.

      • KCI등재

        The effect of the HRB linker of Newcastle disease virus fusion protein on the fusogenic activity

        Liu Yaqing,Liu Ying,Huang Yanan,Wen Hongling,Zhao Li,Song Yanyan,Wang Zhiyu 한국미생물학회 2021 The journal of microbiology Vol.59 No.5

        Newcastle disease, designated a class A disease of poultry by the Office international des epizooties (OIE), is an acute infection caused by Newcastle disease virus (NDV). The merging of the envelope of NDV with the membrane of a target host cell is the key step in the infection pathway, which is driven by the concerted action of two glycoproteins: haemagglutinin- neuraminidase (HN) and fusion (F) protein. When the HN protein binds to the host cell surface receptor, the F protein is activated to mediate fusion. The three-dimensional structure of the F protein has been reported to have low electron density between the DIII domain and the HRB domain, and this electron-poor region is defined as the HRB linker. To clarify the contributing role of the HRB linker in the NDV F protein-mediated fusion process, 6 single amino acid mutants were obtained by site-directed mutagenesis of the HRB linker. The expression of the mutants and their abilities to mediate fusion were analysed, and the key amino acids in the HRB linker were identified as L436, E439, I450, and S453, as they can modulate the fusion ability or expression of the active form to a certain extent. The data shed light on the crucial role of the F protein HRB linker in the acquisition of a normal fusogenic phenotype.

      • KCI등재

        Mutations of ARX and non-syndromic intellectual disability in Chinese population

        Yufei Wu,Huan Zhang,Xiaofen Liu,Zhangyan Shi,Hongling Li,Zhibin Wang,Xiaoyong Jie,Shao-Ping Huang,Fu-Chang Zhang,Junlin Li,Ke-Jin Zhang,Xiao-Cai Gao 한국유전학회 2019 Genes & Genomics Vol.41 No.1

        Mutations of Aristaless-related homeobox (ARX) gene were looked as the third cause of non-syndromic intellectual disability (NSID), while the boundary between true disease-causing mutations and non-disease-causing variants within this gene remains elusive. To investigate the relationship between ARX mutations and NSID, a panel comprising six reported causal mutations of the ARX was detected in 369 sporadic NSID patients and 550 random participants in Chinese. Two mutations, c.428_451 dup and p.G286S, may be disease-causing mutations for NSID, while p.Q163R and p.P353L showed a great predictive value in female NSID diagnosis with significant associations (X2 = 19.60, p = 9.54e−6 for p.Q163R; X2 = 25.70, p = 4.00e−07 for p.P353L), carriers of these mutations had an increased risk of NSID of more than fourfold. Detection of this panel also predicted significant associations between genetic variants of the ARX gene and NSID (p = 3.73e−4). The present study emphasized the higher genetic burden of the ARX gene on NSID in the Chinese population, molecular analysis of this gene should be considered for patients presenting NSID of unknown etiology.

      • KCI등재

        Chitosan-based porous carbon as a support for Zn-based catalysts in acetylene acetoxylation

        Junyu Zhang,Fulong Zhu,Ying Zhang,Mingyuan Zhu,Hongling Li,Bin Dai 한국화학공학회 2022 Korean Journal of Chemical Engineering Vol.39 No.7

        Using the biomass material chitosan as a precursor and potassium citrate (PC) as a substitute for traditionalcorrosive activators such as KOH and ZnCl2, a chitosan-based porous carbon material with high specific surfacearea was successfully prepared and used as a support for the catalytic acetylene acetoxylation reaction. By controllingthe PC content and the calcination temperature, chitosan-based porous carbon with a suitable pore structure and abundantsurface oxygen functional groups was obtained. The inductively coupled plasma analysis confirmed that the zinccontent of the 0.9Zn/CS-PC1-800 catalyst was about 14 wt%, and the acetic acid conversion reached 81%. Furthermore,the scanning electron microscopy and Brunauer-Emmett-Teller (BET) analysis showed that the catalyst carrierwas mesoporous carbon material, and different PC content formed different pore size distribution at different calcinationtemperatures. In addition, X-ray photoelectron spectroscopy analysis demonstrated that the content of O in chitosan-based porous carbon was rich, and PC consumed the O content on the surface of carbon materials duringactivation. Because O content and pore size structure on carrier surface are closely related to acetic acid conversion,reasonable PC content and calcination temperature are very important for acetic acid conversion.

      • Concentric and Spiral Few-Layer Graphene: Growth Driven by Interfacial Nucleation vs Screw Dislocation

        Tay, Roland Yingjie,Park, Hyo Ju,Lin, Jinjun,Ng, Zhi Kai,Jing, Lin,Li, Hongling,Zhu, Minmin,Tsang, Siu Hon,Lee, Zonghoon,Teo, Edwin Hang Tong American Chemical Society 2018 Chemistry of materials Vol.30 No.19

        <P>Spiral growth of various nanomaterials including some two-dimensional (2D) transition metal dichalcogenides had recently been experimentally realized using chemical vapor deposition (CVD). However, such growth that is driven by screw dislocation remained elusive for graphene and is rarely discussed because of the use of metal catalysts. In this work, we show that formation of few-layer graphene (FLG) with a spiral structure driven by screw dislocation can be obtained alongside FLG having a concentric layered structure formed by interfacial nucleation (nucleation at the graphene/Cu interface) using Cu-catalyzed ambient pressure CVD. Unlike commonly reported FLG grown by interfacial nucleation where the second layer is grown independently beneath the first, the growth of a spiral structure adopts a top growth mechanism where the top layers are an extension from the initial monolayer which spirals around an axial dislocation in self-perpetuating steps. Since the same atomic orientation is preserved, the subsequent spiraling layers are stacked in an oriented AB-stacked configuration. This contrasts with FLG formed by interfacial nucleation where turbostratic stacking of the entire adlayer may exist. In both growth scenarios, the second layer (either top or bottom) can grow across the grain boundaries of the initial monolayer domains, forming partial regions with turbostratic stacking configuration due to weak interlayer van der Waals interactions. The unique interlayer coupling of FLG spirals, which enable superior conductivity along the normal of the 2D crystal with spiraling trajectories, are expected to have new and interesting nanoscale applications.</P> [FIG OMISSION]</BR>

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