Di-2-ethylhexyl phthalate induced oxidative damage involving FasL-associated apoptotic pathway in mouse spermatogenic GC-2spd cells

Xiao Tang,Kun Tong,Lishan Zhu,Guoqing Fu,Wei Chang,Ting Zhou,Zhibing Zhang,Ling Tong,Ling Zhang,Yuqin Shi,Ling Zhang,Yuqin Shi 대한독성 유전단백체 학회 2016 Molecular & cellular toxicology Vol.12 No.4

Di-2-ethylhexyl phthalate (DEHP) which belongs to phthalatic-acid esters, is a kind of harmful, global environmental pollutants. It is a known endocrine disrupting chemical and male reproductive toxicant. However, the mechanism by which DEHP exposure result in male reproductive toxicity is still unclear. To elucidate the productive toxicity mechanism of DEHP, we attemptted to investigate oxidative stress, apoptotic effects, mRNA and protein expression of apoptosis-associated genes including FasL, caspase-3, and caspase-8 in GC-2spd (mouse spermatogenic cells). The results showed that, with the increase of DEHP concentration, cell apoptosis rate increased; the activities of relation index of oxidative stress such as malondialdehyde (MDA), superoxide dismutase (SOD) and glutatione peroxidase (GSH-PX) changed significantly; the mRNA and protein expression levels of FasL, caspase-3, -8, altered obviously. These results suggested that DEHP could induce apoptosis of GC-2spd cells through oxidative stress and FasL-dependent pathway.

Fotemustine, Teniposide and Dexamethasone in Treating Patients with CNS Lymphoma

Wu, Jing-Jing,Wang, Xin-Hua,Li, Ling,Li, Xin,Zhang, Lei,Sun, Zhen-Chang,Fu, Xiao-Rui,Ma, Wang,Chang, Yu,Zhang, Xu-Dong,Han, Li-Juan,Zhang, Ming-Zhi Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.11

Purpose: We developed and evaluated a regimen including fotemustine, teniposide and dexamethasone (FTD) for treating patients with central nervous system (CNS) lymphoma based on pharmacokinetic properties of individual agents and in combination. Patients and Methods: In a comparison study, 8 patients with primary CNS lymphoma (PCNSL) and 8 with secondary CNS lymphoma (SCNSL) were treated with FTD (comprising fotemustine 100 mg/m2, 1h infusion, day 1; teniposide 60 mg/m2, >0.5 h infusion, on day 2, 3, 4; dexamethasone 40 mg, 1h infusion, on day 1, 2, 3, 4 and 5; and methotrexate 12 mg, cytosine arabinoside 50 mg plus dexamethasone 5 mg intrathecally, on day 2 and 7). Cycles were repeated every 3 weeks. After response assessment, patients received whole brain radiotherapy. Results: Of the 8 PCNSL patients, 4 (50%) achieved CR and 3 (38%) PR, an overall response rate of 88%. Four patients (50%) were in continuing remission at the end of this study after a median follow-up of 30 months (range 10 to 56 months). Of the 8 SCNSL patients the overall response rate was 63% (CR+PR: 38%+25%). All responses were achievable with predictable toxicity mainly reflecting reversible myelosuppression. Conclusion: This study suggests that FTD could be an effective treatment for CNS lymphoma, and is worthy of further evaluation.

The electronic and magnetic properties with intrinsic defects in ZnO nanosheets: First-principles prediction

Fu-Bao Zheng,Chang-Wen Zhang,Pei-Ji Wang,Hang-Xing Luan 한국물리학회 2013 Current Applied Physics Vol.13 No.4

Using full-potential linearized augmented plane wave (FLAPW) method, we investigated the effects of intrinsic vacancies on electronic and magnetic properties in graphene-like ZnO nanosheets. The results show that the oxygen vacancy (VO) has no influence on magnetism in ZnO nanosheet, whereas the Zn vacancy (VZn) lead to spin polarization of the nanostructures with a total magnetic moment of 2.0mB due to O-2p and Zn-3d hybridization. When the distance of two VZn defects increases to 6.499 A, the system shows an intriguing half-metallic character with 100% spin-polarized carriers due to O(2p)-Zn(3d)-O(2p) coupling chain between two VZn defects.

Studies on PEGylated Gold Nanoparticles Loaded with 5-Hydroxydecanoate for Chemo-Photothermal Therapy of Human Lung Adenocarcinomas In Vitro

Fu-Yan Zhuge,CONG-WANG ZHANG,CHANG-CHUN ZENG,Han-Ping Liu 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2015 NANO Vol.10 No.7

Gold nanoparticles (Au NPs) have promising applications in the fields of drug delivery and photothermal therapy. 5-hydroxydecanoate (5-HD) is a kind of highly selective mitochondrial ATP sensitive potassium (mitoKATP) channel blocker. In this research, firstly, 5-HD was studied whether it could induce human lung adenocarcinomas (A549) cells apoptosis; secondly, PEGylated gold nanoparticles loaded with 5-HD (Au NPs-PEG-5-HD) were prepared to develop a new chemotherapy and photothermal therapy in one system under the irradiation of green light-emitting diode (LED). Subsequently, in vitro cytotoxicity test was analyzed by cell counting kit-8 (CCK-8), the change of mitochondrial membrane potential (Δψm) was determined by immunofluorescence microscopy with R-123 fluorescence, and cell apoptosis and necrosis rate were detected by flow cytometry. The results of CCK8 revealed that the inhibition rates of A549 cells were all greatly increased when cells were treated with free 5-HD, free 5-HD +LED irradiation, Au NPs-PEG-5-HD and Au NPs-PEG-5-HD+LED irradiation, and Au NPs-PEG-5-HD had enhanced cell-killing effect compared with 5-HD, furthermore, the Au NPs-PEG-5-HD and LED irradiation played a very great synergy effect. The immunofluorescence microscopy data also exhibited a reduction of Δψm correspondingly. Flow cytometric analysis showed that the apoptosis rate of cells that were incubated with free 5-HD, 5-HD+LED irradiation, Au NPsPEG-5-HD or Au NPs–PEG–5-HD+LED irradiation significantly increased to about 9.2%, 10.7%, 18.3% or 12.4% and the percentage of necrosis cells increased to around 8.8%, 9.7%, 48.0% or 69.8%, respectively. In a word, all the results indicated that the 5-HD had shown the ability to induce A549 cells apoptosis as a chemotherapy agent, and its ability would be improved when 5-HD is loaded on PEGylated Au NPs, as well as Au NPs-PEG-5-HD exhibited significantly enhanced photothermal effects for treatment of lung adenocarcinomas.

Multi-Receiver Quantum Dense Coding with a Non-Symmetric Quantum Channel

Chang-Bao Fu,Shou Zhang,엄정인,Ke-Li,연규황 한국물리학회 2006 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.48 No.5I

A two-receiver quantum dense coding scheme and an N-receiver quantum dense coding scheme, in the case of non-symmetric Hilbert spaces of the particles of the quantum channel, are investigated in this paper. A sender can send his messages to many receivers simultaneously. The scheme can be applied to quantum secret sharing and controlled quantum dense coding.

Lack of Influence of an XRCC3 Gene Polymorphism on Oral Cancer Susceptibility: Meta-analysis

Zhang, En-Jiao,Cui, Zhi-Gang,Xu, Zhong-Fei,Duan, Wei-Yi,Huang, Shao-Hui,Tan, Xue-Xin,Yin, Zhi-Hua,Sun, Chang-Fu,Lu, Li Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.23

Background: To systematically summarize the association between the X-ray repair cross complementing 3 (XRCC3) gene polymorphism and oral cancer susceptibility by meta-analysis. Materials and Methods: Databases including PubMed, EMbase, CNKI, VIP and WanFang Data were searched to identify case-control studies concerning the association between an XRCC3 gene polymorphism and the risk of oral cancer from the inception to June 2014. Two reviewers independently screened the literature according to the criteria, extracted the data and assessed the quality. Then meta-analysis was performed using Stata 11.0 software. Results: Seven published case-control studies including 775 patients with oral cancer and 1922 controls were selected. Associations between the rs861539 polymorphism and overall oral cancer risk were not statistically significant in all kinds of comparison models (CT vs CC: OR=0.94, 95%CI=0.74-1.18; TT vs CC: OR=0.94, 95%CI=0.64-1.38; dominant model: OR=0.95, 95%CI=0.76-1.18; recessive model: OR=0.94, 95%CI=0.69-1.29; allele T vs C: OR=0.97, 95%CI=0.84-1.11). In the stratified analysis by ethnicity, no significant associations were found among Asians and Caucasians. On stratification by tumor type, no significant associations were found for cancer and oral premalignant lesions. Conclusions: The XRCC3 gene polymorphism was not found to be associated with the risk of oral cancer. Considering the limited quality of the included case-control studies, more high quality studies with large sample size are needed to verify the above conclusion.

Association of CYP2E1 and NAT2 Polymorphisms with Lung Cancer Susceptibility among Mongolian and Han Populations in the Inner Mongolian Region

Zhang, Jing-Wen,Yu, Wan-Jia,Sheng, Xiao-Min,Chang, Fu-Hou,Bai, Tu-Ya,Lv, Xiao-Li,Wang, Guang,Liu, Su-Zhen Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.21

Purpose: To explore associations of CYP2E1 and NAT2 polymorphisms with lung cancer susceptibility among Mongolian and Han populations in the Inner Mongolian region. Materials and Methods: CYP2E1 and NAT2 polymorphisms were detected by PCR-RFLP in 930 lung cancer patients and 1000 controls. Results: (1) Disequilibrium of the distribution of NAT2 polymorphism was found in lung cancer patients among Han and Mongolian populations (p=0.031). (2) Lung cancer risk was higher in individuals with c1, D allele of CYP2E1 RsaI/PstI, DraI polymorphisms and slow acetylation of NAT2 (c1 compared with c2, OR=1.382, 95%CI: 1.178-1.587, p=0.003; D compared with C, OR=1.241, 95%CI: 1.053-1.419, P<0.001; slow acetylation compared with rapid acetylation, OR=1.359, 95%CI:1.042-1.768, p=0.056) (3) Compared with c2/c2 and rapid acetylation, c1/c1 together with slow acetylation synergetically increased risk of lung cancer 2.83 fold. (4) Smokers with CYP2E1 c1/c1, DD, and NAT2 slow acetylation have 2.365, 1.916, 1.841 fold lung cancer risk than others with c2/c2, CC and NAT2 rapid acetylation, respectively. (5) Han smokers with NAT2 slow acetylation have 1.974 fold lung cancer risk than others with rapid acetylation. Conclusions: Disequilibrium distribution of NAT2 polymorphism was found in lung cancer patients among Han and Mongolian populations. Besides, Han smokers with NAT2 slow acetylation may have higher lung cancer risk compared with rapid acetylation couterparts. CYP2E1 c1/c1, DD and NAT2 slow acetylation, especially combined with smoking, contributes to the development of lung cancer. CYP2E1 c1/c1 or DD genotype and NAT2 slow acetylation have strong synergistic action in increasing lung cancer risk.

Anthocyanins in ‘Cabernet Gernischet’ (Vitis vinifera L. cv.) Aged Red Wine and Their Color in Aqueous Solution Analyzed by Partial Least Square Regression

Fu-Liang Han,Shou-Mei Jiang,Jian-Jun He,Qiu-Hong Pan,Chang-Qing Duan,Ming-Xia Zhang 한국식품과학회 2009 Food Science and Biotechnology Vol.18 No.3

Anthocyanins are considered one of the main color determinants in aged red wine. The anthocyanins in aged red wine made from ‘Cabernet Gernischet’ (Vitis vinifera L. cv.) grape were investigated by high performance liquid chromatography- electronic spray ionization- mass spectrometry (HPLC-ESI-MS) and their color presented in aqueous solution were evaluated using partial least square regression (PLS). The results showed that there were 37 anthocyanins identified in this wine, including 22 pyranoanthocyanins. The analysis of PLS indicated that different anthocyanins showed distinct color values: malvidin 3-O-(6-O-acetyl)-glucoside-4-vinylguaiacol (Mv3-acet-glu-vg) presented the highest color values, while malvidin 3-O-glucoside (Mv3-glu) showed least. Among the free non-acylated anthocyanins, peonidin 3-Oglucoside (Pn3-glu) showed the highest color values; the coumarylated anthocyanins presented higher color values than their corresponding acetylated anthocyanins and parent anthocyanins; pyranoanthocyanins presented also higher color values than their original anthocyanins; the color of anthocyanins depended on their structure. This work will be helpful to reveal evolution in aged red wine.

Preparation of Well-Dispersed Nanosilver in MIL-101(Cr) Using Double-Solvent Radiation Method for Catalysis

SHUQUAN CHANG,Chengcheng Liu,Heliang Fu,Zheng Li,Xian Wu,Jundong Feng,Haiqian Zhang 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2018 NANO Vol.13 No.12

In this study, a double-solvent radiation method is proposed to prepare silver nanoparticles in the pores of metal-organic framework MIL-101(Cr). The results reveal that well-dispersed silver nanoparticles with a diameter of about 2 nm were successfully fabricated in the cages of monodisperse octahedral MIL-101(Cr) with a particle size of about 400 nm. The structure of MIL-101(Cr) was not destroyed during the chemical treatment and irradiation. The resulting Ag/MIL-101 exhibits excellent catalytic performance for the reduction of 4-nitrophenol. This method can be extended to prepare other single or bimetallic components inside porous materials.

Fangchinoline Inhibits Cell Proliferation Via Akt/GSK-3beta/cyclin D1 Signaling and Induces Apoptosis in MDA-MB-231 Breast Cancer Cells

Wang, Chang-Dong,Yuan, Cheng-Fu,Bu, You-Quan,Wu, Xiang-Mei,Wan, Jin-Yuan,Zhang, Li,Hu, Ning,Liu, Xian-Jun,Zu, Yong,Liu, Ge-Li,Song, Fang-Zhou Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.2

Fangchinoline (Fan) inhibits cell proliferation and induces apoptosis in several cancer cell lines. The effects of Fan on cell growth and proliferation in breast cancer cells remain to be elucidated. Here, we show that Fan inhibited cell proliferation in the MDA-MB-231 breast cancer cell line through suppression of the AKT/Gsk-3beta/cyclin D1 signaling pathway. Furthermore, Fan induced apoptosis by increasing the expression of Bax (relative to Bcl-2), active caspase 3 and cytochrome-c. Fan significantly inhibited cell proliferation of MDA-MB-231 cells in a concentration and time dependent manner as determined by MTT assay. Flow cytometry analysis demonstrated that Fan treatment of MDA-MB-231 cells resulted in cell cycle arrest at the G1 phase, which correlated with apparent downregulation of both mRNA and protein levels of both PCNA and cyclin D1. Further analysis demonstrated that Fan decreased the phosphorylation of AKT and GSK-3beta. In addition, Fan up-regulated active caspase3, cytochrome-c protein levels and the ratio of Bax/Bcl-2, accompanied by apoptosis. Taken together, these results suggest that Fan is a potential natural product for the treatment of breast cancer.