신경세포성장인자로서 척수운동신경세포의 손상에 미치는 한약재의 약류별 효능 및 기전에 관한 연구

鄭遇悅,朴承澤,成彊慶,李星根 대한동의병리학회 1998 동의생리병리학회지 Vol.12 No.2

산소자유기가 흰쥐의 배양 운동신경세포에 미치는 세포독성에 대한 기전을 규명하기 위하여 여러 농도의 산소자유기를 배양 신경세포에 처리한 후 산소자유기의 신경독성 효과를 분석하였으며 또한 산소자유기에 의하여 유발된 신경독성에 대한 한약재중 보혈약류와 보기약류의 대표적인 처방인 사군자탕과 사물탕의 신경세포독성의 방어효과를 MTT assay법에 의하여 조사하여 다음과 같은 결론을 얻었다. Xanthine oxidase(XO)와 hypoxanthine(HX)은 처리한 농도에 비례하여 배양 신경세포의 생존율을 현저하게 감소시켰으며, 또한 사물탕과 사군자탕이 XO/HX의 독성효과를 유의있게 방어하였다. 이상의 결과로부터 산소자유기는 배양 소의 희소돌기아교세포에 독성을 나타냈으며 사군자탕과 사물탕등의 한약추출물이 산소자유기의 독성을 방어하는데 효과적인 것으로 나타났다. In order to elucidate the neuroprotective effect of Sagunjatang and Samultang on oxidant-induced neurotoxicity in cultured rat spinal motor neurons, the colorimetric assay such as MTT assay was performed and light microscopic study was also carried out after cultured spinal motor neurons from neonatal rat were treated with the medium containing various concentrations of xanthine oxidase(XO) and hypoxanthine(HX) for 3 hours. In addition, protective effect of herb extracts on the neurotoxicity induced by oxygen radicals was examined in these cultures. The results were as follows : Cell viability of cultured rat spinal motor neurons which were exposed to various concentrations of 30mU/ml XO and 0.1mM HX for 3 hours, was decreased in a dose-dependent manner. MTT50 value was 30mU/ml XO and 0.1mM HX after motor neurons were incubated with various concentrations of XO/HX for 3 hours. Cultured spinal motor neurons showed degenerative changes such as the decrease of cell number and loss of cell processes when neurons were cultured in the medium containing 30mU/ml X0 and 0.1mM HX for 3 hours. Oxygen radicals were toxic on cultured rat spinal motor neurons by the decrease of cell viability. Herb extracts, Sagunjatang and Samultang prevented the oxidant-induced neurotoxicity significantly after spinal motor neurons were preincubated with herb extractions for 2 hours before exposure of oxygen radicals. These results suggest that oxygen radicals have neurotoxic effect on cultured spinal motor neurons derived from neonatal rat, and selective herb extracts such as Sagunjatang and Samultang are very effective in protecting the neurotoxicity induced by oxygen radicals.

고선량 방사선 조사후 치아수복재의 특성변화

이용근,이건일,이성재,박수아,허정무 원광대학교 생체재료·매식연구소 1996 원광생체재료·매식 Vol.5 No.2

To investigate the effect of high dose irradiation on the Vickers hardness number(VHN), color change and wear rate of dental filling materials, esthetic filling material specimens were studied. Six kinds of composite resins and two kinds of glass ionomers(GI) were used. One(CLF) was chemically cured composite resin, and five(HCX, HPL, PHB, POF, PRT and RZI) were light cured composite resins. One(FJT) of GI was chemically cured GI and one(FLC) was light cured GI. The filling material specimens were made according to the manufacture's instructions and were finally polished with Microcloth(Buehler). Half of the specimens were irradiated with 100 Gy radiation using a linear accelerator(Mitsubishi, 6 MV). VHN and CIELAB color were measured before and after irradiation, and wear test were performed for nonirradiated specimens by brushing with toothpaste, From the experiment , the following results were obtained. 1. The CIELAB color difference was significantly different depending on the material(p<0.01), and FLC showed the highest color difference (CIELAB ΔE) of 9.452 and CLF showed the lowest value of 3.642. 2. The VHNs of nonirradiated specimens were 12.22∼73.58 and those of irradiated specimens were 25.53∼84.53. The VHNs of irradiated specimens were significantly higher than those of nonirradiated specimens(p<0.05) except RZI, FJT and FLC. 3. The wear rate of irradiated specimens of RZI, FLC were significantly lower than those of nonirradiated specimens(p<0.05). 4. The color difference after brushing of irradiated specimens were higher than those of nonirradiated specimens except CLF.

알코올의 진정 효과와 일부 인지 기능에 미치는 영향

호정일,왕성근 대한생물치료정신의학회 2003 생물치료정신의학 Vol.9 No.1

Objectives : The purpose of this study was to evalute the effect of alcohol on sedation and some cognitive function(eg,daytime sleepiness, fatigue, and mood measured by multiple sleep latency test(MSLT) and visual analogue scale(VAS), vigilance, attention and short-term memory measured by Vienna Test System). Methods : 15 healthy, normal young college students participated in this study. Participating subjects were in good physical and psychological health and were asymptomatic as to sleep/wake complaints. Subjects visited our laboratory by 08 : 00 at the first day, and they had baseline MSLT and Test(at 9;00, 11;00, 14:00, and 16:00).Each time, the subjects completed VAS for sleepiness, fatigue and mood before they underwent an Vienna Test to evaluate their vigilance, attention and short-term memory. On the second day, subjects had 0.5g/kg of alcohol, the third day, subject had 1.0g/kg of alcohol, and repeated the same process as those of the first. Results : 0.5g/kg of alcohol reduced sleep latency after 1 hour, and 1.0g/kg of alcohol reduced sleep latency until 5 hours after administration significantly. 0.5g/kg of alcohol induced daytime sleepiness 1 hours after administration, and 1.0g/kg of alcohol induced daytime sleepiness and fatigue until 3 hours after administration, but there were no effects on mood. Vigilance was decreased significantly 1 hour after 0.5g/kg of alcohol administration, and attention and short-term memory were not changed significantly after alcohol administration. After alcohol adminstration(0.5-1.0g/kg), there were significant positive correlation between daytime sleepiness and fatigue, and fatigue and mood. Conclusion : It can be concluded that alcohol decreases sleep latency and induces daytime sleepiness and fatigue, and decreases vigilance in normal subjects.

Frozen Shoulder에 있어서 마사지의 치료적 접근

홍성찬,김영빈,박병근,정동혁,이정윤,이동헌 圓光大學校 附設 體力科學硏究所 2003 體力科學硏究 Vol.26 No.1

Painful stiffness of the shoulder is an ill-defined clinical entity that is difficult to assess and delicate to treat. The nomenclature used is broad and includes terms such as frozen shoulder, adhesive capsulitis, focal algodystrophy, stiff shoulder, contracted shoulder, and others. Apart from its idiopathic form, the disease can be initiated by trauma, infection, tumour, radiation, systemic and local metabolic disturbances. Pathoanatomically, the common denominator is an inflammatory vascular proliferation followed by thickening, scarring, and retraction of the joint capsule. The inflammatory process often starts at the rotator interval and may extend to the subacromial space. Clinical diagnosis is based on history and physical examination. Generally the onset of pain precedes the perception of a reduced range of motion by weeks or months. In early stages of the disease, the inflammatory type of pain dominates, i.e., the patient's main complaint ist pain at night. In the later stage, range of motion gradually decreases. Patients do not often complain about reduced motion, probably because of its slow onset. The purpose of this study is to find out the effectiveness of the massage applied to the patients with Frozen Shoulder by measuring, assessing and analyzing the changes in intensity and unpleasantness of pain and range of motion(ROM) before and after massage. Surveyed from Jan. 2001 to December 2002 were 104 patients suffering from Frozen Shoulder. In the therapeutic massage program, such methods as Effleurage, Petrissage and Deep transverse friction were selected as they are among traditional massage treatments frequently used for joints with pain and restricted ROM. Effleurage and Petrissage were applied for 20 minutes in total before and after Deep Transverse Friction. After massage program, the intensity and unpleasantness of pain were measured and analyzed by means of VAS(visual analogue scale)-I and VAS-U, while ROM was measured and analyzed by using Goniometer. The major findings from this study are as follows; 1. The surveyed patients range from 28 to 75 in age, with highest numbers of 44(42.31%) registered in the fifties and next ones of 24(23.08%) in the forties. Divided by sex, 59 are women and 45 are men totalling 104 with average age of 51.4. 2. There was significant decrease in the intensity of pain and unpleasantness of pain after massage(p<.05). 3. There appeared, however, significant increase in ROM after massage(p<.05). 4. From the analysis into chronological changes in ROM before and after massage with ANOVA, it became evident that the longer the period of treatment was, the higher the ROM increases drastically, while significant difference was shown in Abduction and in External and Internal Rotation(p<.05). Summed up, it can be generally concluded that massage is an effective treatment to rid the patients with Frozen Shoulder of pains safely and promptly. This study was conducted to determine more accurate impact of massage. From the above outcomes, it was revealed that massage has proven to reduce the pains, exercising huge influence to increase ROM. It is, therefore, suggested to continue and expand the study on the cure of Frozen Shoulder and to motivate patients, orthopedists, physiatrists, physiotherapists, and sports massagists etc. to consider massage as safest and most efficient pain remedy.

한국인 성인에서 Fm θ의 출현 양상에 관한 연구

도정수,신석철,왕성근 충남대학교 의과대학 지역사회의학연구소 1997 충남의대잡지 Vol.24 No.2

The distinctive theta rhythm that appears at the frontal midline during the performance of mental tasks has been designated as frontal midline theta(Fmθ). Author tried to confirm the appearance pattern of Fmθ in Koean young adults, and investigated the relations among the appearance rates of Fmθ, sex, kinds of mental tasks, characteristics of personality trait, impulsiveness, temperament, and anxiety level. The subjects were 42 healthy male and female students(28 male, 14 female) aged 23 to 25(average age 23.9±0.6). The results were summarized as follows. 1. The individual variation of the appearance and appearance rates of Fmθ were large, and the appearance rates of Fmθ under Uchida-Kraepelin test were ranged 28.6~31.0%, and under reaction time were ranged 4.8%~21.4%. 2. The appearance rates of Fmθ tend to increase under continuous mental task than simlpe concentration. 3. There were no sexual difference in the apearance rate of Fmθ. 4. In the aspect of the anxiety level, the appearance rate of Fmθ seems to bo increased when the person's level of anxiety is low, the excitement strength is high, and less impulsive. 5. In the aspect of the personality, the appearance rate of Fmθ seems to be increased when the person's personality is introvert, the excitement strength is high, and the motility of temperament is high.

스페로이드 조합자극치료 적용 후 만성요통의 통증 감소 효과

홍성찬,김영빈,박병근,김유성,이성진,정동혁 원광대학교 체력과학연구소 2008 體力科學硏究 Vol.30 No.1

The aim of the present study is to evaluate effects of Spheroid 9000NS applied to 10 elderly patients with Chronic Low Back Pain. The effects were measured and analyzed by means of MPQWL, VRS, VAS-I and VAS-U after 4, 8 and 12 weeks, respectively, during the 12-week application of the Spheroid 9000NS. From the findings of the measurement and analysis, following conclusion could be drawn: 1. Significant reductions in MPQWL were confirmed at each period of measurement since the use of Spheroid 9000NS(p<.001). 2. Significant reductions were also revealed in VRS at each period of the measurement(p<.001). 3. Significant reductions were also revealed in VAS-I at each period of the measurement(p<.001). 4. The same reductions were found as well in VAS-U 4, 8 and 12 weeks after the application of the Spheroid 9000NS(p<.001). In sum, it may be concluded from the outcomes that the use of Spheroid 9000NS may help reduce drastically Chronic Low Back Pain, mostly effective to remove them speedily and safely. Patients, rehabilitation therapists and health experts are, therefore, recommended to employ the products as safe and effective means to control pains. Continued researches and experiments should further be conducted to collect more data from the larger population of patients with particular diseases in clinical situation, though.

정상 성인에서 수면박탈이 수면구조에 미치는 영향

왕성근,이정규,최하석 충남대학교 의과대학 지역사회의학연구소 1991 충남의대잡지 Vol.18 No.2

To identify the effect of total sleep deprivation on sleep structures, polysomnography was performed for 8 healthy young adults. The subjects were young adults, ranging in age from 21 years to 26 years, with no past or present histories of significant medical, neurological, or psychiatric illness as well as no current major sleep disturbances or parasomnias, After one adaptation night, each subject was recorded polysomnography in the sleep laboratory. Sleep records were analyzed according to the criteria of Rechtschaffen and Kales every 20 seconds, and tried paired-t-test. The results were summarized as follows. 1. After on night of total sleep deprivation, compared with the baseline WASO was significantly decreased in the first recovery night, and SWS and REM sleep were significantly increased in the first recovery night. 2. Compared with the baseline, the relative value(%) of REM sleep was significantly increased in the first recovery night. 3. Compared with the baseline, TST was significantly increased in the first recovery night, and sleep latency and REM latency were significantly decreased in the first recovery night. According to the above results we could identify the effects of total sleep deprivation on sleep structure in normal young adults.

만성 정신분열증 환자의 정보처리에 관한 실험적 연구

도정수,왕성근 大韓神經精神醫學會 1991 신경정신의학 Vol.30 No.2

To assess whether the information processing dysfunction in schizophrenics occurs at preattentional level or attentional level, the authors investigated information processing in schizophrenia with 40 schzophrenics diagnosed by DSM-III_R and 40 normal males. The basic methodology relied on Treisman's feature-integration theory and the visual search task. Slopes and intercepts of response time which wee obtained by linear regression, and response times between two groups were analysed by MANOVA and the results were obtained as follows. 1) The slopes of response times of the schizophrenics exceed those of controls and the slopes of schziophrenics in conjunction condition exceed the slopes of schizophrenics in feature condition. The above results suggest that information processing dysfunction in chronic schizophrenics occurs at both preattentional level and attentional level but more at attentional level. 2) Intercepts of response times of the schizophrenics exceed those of controls, which suggest that schizophrenics were also impired at the early stage of informational processing. The above results suggest that information processing dysfunction in chronic schziophrenics occurs both at preattentional level and attentional level but more at attentional level.

유산소성 운동이 심폐기능과 체지방에 미치는 영향

홍성찬,김영빈,박병근,정동혁,이정윤,김유성 圓光大學校 附設 體力科學硏究所 2005 體力科學硏究 Vol.28 No.-

The Purpose of this research is to examine the effect of the aerobic exercise duration on cardiorespiratory function and body fat. Total 14 divided into two groups 7, for the control group and the other for the experimental group 7. The subjects of experimental group had 8 weeks aerobic program in the given intensity, during which the changes in cardorespiratory function and body fat were measured three times, at each end of 0th, 8th week. The research is comcluded as follows. 1. After 8 Weeks, aerobic exercise brought the decrease of maximum HR and the increase of VE with statistically significant(p<.05). 2. Aerobic exercise brought along the change of fat, especially the decline of the rate of fat and the amount of fat. In conclusion, aerobic exercise helps overweight women to reduce their weights and the amount of fat and to get rid of elements that cause various geriatric diseases. Therefore, overweight women as well as common adults are advised to take aerobic exercises

Streptomyces sp. Y9343이 生産하는 齒面細菌膜 分解酵素의 精製와 特性

김성주,한홍근,윤정원 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.1

치면세균막을 제거하거나 형성을 억제하여 치아우식증 예방제를 개발할 목적으로 α-1,3 glucanase를 분비하는 새로운 균주로 부터 분리 정제하여 그 특성을 조사하였다. 불용성 glucan을 유일탄소원으로 하는 agar plate를 제조하여 토양으로 부터 α-1,3 glucanase 분비균주를 탐색한 결과 Streptomyces sp. Y9343을 얻었다. 액체배양시 최적 효소생산조건은 탄소원으로 1% soluble starch와 indcer로 0.5% insoluble glucan을 첨가하였을 때 가장 효율적이었다. α-1,3 glucanase는 황산 암모늄 염석, DEAE-CEllulose 이온교환 크로마토그래피, Sephadex G-75 겔 여과 등에 의하여 32.1배까지 정제되었고 수율은 0.53%이었으며, 이 때의 활성도는 7840.9 U/mg protein이었다. 정제된 α-1,3 glucanase를 SDS-PAGE로 분석한 결과, 단일체임을 확인하였으며, 이 때 분자량은 22,500이었다. 효소의 최적 pH는 6.5이었다. 효소의 최저온도는 37℃이었고, 열에 대한 안정성은 70℃ 이상에서 40%~60%의 효소활성이 상실함을 보였다. Detergent의 영향은 SDS에 의해 83%, Tween 20에 의해서는 약 27% 정도의 활성저해를 받았다. 효소활성의 금속이온에 의한 영향은 Co^2+, Mn^2_+에 의해 각각 81.8, 69.7%의 활성의 증가를 보였고 이들의 최적농도는 10mM이었으며, 반면에 Hg^2+에 의해서는 93.9%의 효소활성의 저해를 나타내었다. 또한 초기속도(30분 이내)에 금속이온에 의한 영향이 큰 것으로 나타났다. α-1,3 glucanase의 불용성 glucan에 대한 K_m 값은 2.50mM이었고, V_max는 0.0431 mM/min이었다. α-1,3 glucanase의 기질특이성을 조사한 결과, 반응 30분 후 IG와 soluble starch에는 각각73, 100%의 높은 분해력을 보였으며, raw starch, dextran T-10에 대해서는 낮은 분해력을 보였다. 한편, 인조치면세균막을 S. mutans로부터 시험관 벽에 제조한 후, α-1,3 glucanase를 처리한 결과 2시간 이내에 완전히 분해 제거되는 것을 알 수 있어 강력한 치아우식예방제로 개발될 수 있음을 보여 주었다. Streptococcus mutans has been implicated as primary causative agents of dental caries by insoluble glucan (IG) in human and experimental animals. An attempt was made to search for the α-1,3 glucanase that degrades IG produced by S. mutans. α-1,3 glucanase was detected in the culture supernatant of microorganisms, which are isolated from soils on agar medium containing IG as a sole carbon source. This Streptomyces sp. hydrolysed IG produced by immobilized S. mutans and was named as Y9343. This enzyme required α-1,3 glucan (IG) as an inducer. The optimum conditions for enzyme production were studied. The enzyme was purified by 30~70% (NH_4)_2SO_4 precipitation, anion exchange chromatography on DEAE-cellulose and gel filration on Sepadex G-75. The purified enzyme has a specific activity of 7840.0 U/mg protein giving 32.1-fold purification and final yield of 0.53%. The molecular weight was estimated to be about 22.5 kDa by SDS-PAGE. The optimum pH and temperature for enzyme reactiorr were 6.5 and 37℃, respectively and the enzyme was relatively stable at the temperature below 60℃. The activity of purified enzyme was enhanced by adding Co^2+, Mn^2+, and Mg^2+ into the medium, whereas inhiited by adding Hg^2+, Zn^2+ and SDS. The K_m and V_max value of α-1,3 glucanase for IG were estimated to be 2.50 mM and 0.0431 mM/min, respectively. The thin layer chromatographic analysis of hydrolysates from IG with α-1,3 glucanase showed that glucose was the main product of reaction. This enzyme activity was about 14 times higher than marketing dextranase as preventive agent against artificial dental caries by S. mutans in TH medium including 5% sucrose after 30 minutes.