The effect of neuropeptides on secretion of Interleukin-8(IL-8)

Kim, Kyung-Jun,Park, Sang-Hyuk,Choi, Kyoung-Kyu,Park, Sang-Jin 大韓齒科保存學會 2006 Restorative Dentistry & Endodontics Vol.31 No.3

본 연구는 치수조직, 치은, 치주인대로부터 배양된 조직을 SP (Substance P)로 4시간, SP, CGRP (Calcitonin Gene-related Peptide), Tumor necrosis factor-α (TNF-α)로 8시간 자극 후 RNase Protection Assay를 시행하고, IL-8의 분비량을 측정해 다음 결과를 얻었다. 1. IL-8 mRNA는 모든세포에서 발현됐다. 2. IL-8 mRNA 발현은 SP (10^(-5)M)와 SP (10^(-8)M)로 4시간 자극 시 증가되지 않았다. 3. IL-8 mRNA 발현은 SP (10^(-4)M)와 CGRP (10^(-6)M)로 8시간 자극 시 증가되지 않았다. 4. TNF-α (2 ng/㎖) 자극 시, IL-8 mRNA 발현이 증가됐다. 5. 치은 세포를 CGRP (10^(-6)M)로 8시간 자극 시, IL-8 분비량이 증가했다 (p < 0.05). 6. 치주인대 세포를 SP (10^(-4)M)로 8시간 자극 시 IL-8 분비량이 증가했다 (p < 0.05). We investigated the secretion of Interleukin-8 (IL-8) from ginviva and periodontal ligament stimulated with Substance P (SP) and Calcitonin Gene-related Peptide (CGRP). Gingiva (GF), periodontal ligament (PDLF) and pulp (PF) tissues were collected from extracted instact 3rd molars. Cultured cells were stimulated with different concentrations of SP for 4 hrs, and stimulated with SP, CGRP and Tumor Necrosis Factor-α (TNF-α) for 8 hrs. Then RNase Protection Assay was carried out. ELISA was performed using supernatants of stimulated cells for quantitative analysis of IL-8. Results were assessed using supernatants of stimulated cells for quantitative analysis of IL-8. Results were assessed using student t-test with significance of P < 0.05. According to this study, the results were as follows: 1. IL-8 mRNA was detected in all type of cells studied (PF, GF and PDLF). 2. IL-8 mRNA expression was not increased after stimulating 4 hrs with SP (10^(-5)M) and SP (10^(-8)M) compared with Mock stimulation in all type of cells studied. 3. IL-8 mRNA expression was not increased after stimulating 8 hrs with SP (10^(-4)M) and CGRP (10^(-6)M) compared with Mock stimulation in all type of cells studied. 4. TNF-α (2 ng/㎖) increased the expression of IL-8 mRNA in all kind of cells studied. 5. The secretion of IL-8 from GF was increased 8 hrs after the stimulation with CGRP (10^(-6)M) (p < 0.05). 6. The secretion of IL-8 from PDLF was increased 8 hrs after the stimulation with SP (10^(-4)M) (p < 0.05). Calcitonin Gene-related Peptide (CGRP) increased Interleukin-8 (IL-8) which plays an important role in chemotaxis of neutrophil in Calcitonin Gene-related Peptide (CGRP) gingival tissue, whereas Substance P increased the secretion of IL-8 from periodontal ligament.

척추질환 입원환자의 연도별 변동추이에 대한 통계적 고찰

박경수 ( Kyung Su Park ),이준환 ( Jun Hwan Lee ),정석희 ( Seok Hee Chung ) 한방재활의학과학회 2006 한방재활의학과학회지 Vol.16 No.4

Objectives : The aim of this study was to find the change in the number of inpatient with spine disease, data were reviewed on inpatients who was diagnosed as spine disease in the Kyung-hee Medical Center(KHMC) for 25 years(1980~2004). Methods : Analysis data of the number of spinal disease was based on the KHMC annual report for 25 years(1980~2004). Results : 1. The number of inpatient in KHMC with spine disease has been increasing during 25years from 1980 to 2004(p-value:0.000) 2. The number of inpatient in KHMC with ankylosing spodylitis(AS) is increasing during 25years from 1980 to 2004(p-value=0.000) 3. The number of inpatient in KHMC with spinal stenosis is increasing during 25years from 1980 to 2004(p-value=0.000) 4. The number of inpatient in KHMC with herniated nucleus pulposus of cervical spine(HNP of C-spine) is increasing during 25years from 1980 to 2004(p-value=0.000) 5. The number of inpatient in KHMC with herniated nucleus pulposus of Lumbar spine(HNP of L-spine) is increasing during 25years from 1980 to 2004 (p-value=0.007) Conclusions : It is suggesting that the number of patients of spinal stenosis and HNP of L-spine increase at KHMC.

BCG와 levamisole 투여가 canine parvovirus subunit antigen에 대한 기니픽의 면역반응에 미치는 영향

박선경,장경수,박종현,전무형 충남대학교 수의과대학 동물의과학연구소 1995 動物醫科學硏究誌 Vol.3 No.-

To investigate the effects of priming with BCG and levamisole on the immune responses of the guinea pigs inoculated with CPV-intact antigen(CPV-IA) and recombinant CPV-subunit antigen(CPV-SA), hemagglutination inhibition(HI) test and leucocytes migration inhibition(LMI) test were applied for measurement of antibody and cell-mediated responses, respectively. 1. The minimal concentration of CPV-IA and CPV-SA to evoke HI antibody in guinea pig was 1,000 HAU and 2,000 HAU, respectively. 2. As the effects of priming with BCG and levamisole were examined by HI test, double primings showed higer HI titers than single priming and the groups primed with levamisole showed higher HI antibody titers than groups primed with BCG. All of groups primed with BCG and levamisole revealed prominantly higher HI titers than the non-primed group. 3. The optimal conditions for LMI were 64 HAU for antigens, 1×10^6 cell/ml for the concentration of leucocyte and 2 hrs for antigen-leucocyte reaction time. 4. When the cellular immune responses for the guinea pig inoculated with CPV-IA were examined by LMI test, BCG-primed groups showed significantly higher %LMI than the levamisole-primed group(P<0.05). Double primings revealed a tendency of higher %LMI than single priming, but statistically not significant. All of primed groups showed significantly higher %LMI than non-primed groups (P<0.001). 5. When the cellular immune responses for the guinea pig inoculated with CPV-SA were examined by LMI test, BCG-primed groups showed a tendency of higher %LMI than the levamisole-primed groups and double primings revealed higher %LMI than single priming in both of BCG and levamisole. All of priming groups showed significantly higher %LMI than the non-primed groups (P<0.001).

유연성을 갖는 로보트 매니퓨레이터의 동적방정식 시뮬레이션에 의한 분석

朴鍾國,배준경,최호선 慶熙大學校 1987 論文集 Vol.16 No.-

High performance requirements such as high speed operation, accuracy and versatility have led to the considration of structural flexibility in robot manipulator. The purpose of this paper is to investigate the interrelationships between the robot structural flexibility and a linear controller for the rigid body motion. The controller used here is used to simulate the controllers currently used in industrial robots. The simulation results illustrate the differences between leadscrew driven and unconstrainted axes of the robot: they indicate the trade-off between speed and accuracy.

중금속오염을 고려한 온산만 조간대 및 아조대의 무척추동물 군집분석

박중기,이인숙,박경숙,송준임,최병래,노분조 이화여자대학교 환경문제연구소 1997 이화환경연구 Vol.1 No.-

한국 동해연안의 하나인 온산만 지역의 해양오염 실태를 파악하기 위해 저서동물 군집구조에 대한 정성, 정량적 분석을 실시하였다. 분석결과 조사지역별 습중량-개체수의 누적 κ-dominance curve 변화양상을 근거로 판단해 분석한 결과, 온산만의 내만에 위치한 춘도는 여름, 겨울 오후에 ()어서 오염지역으로, 대조군인 당사는 비오염지역,()각각 판정되었다. 이외에도 조사지역별 출현() () 있어서 대조구인 당사지역에 비해 춘도의 생물상이 ()대적으로 빈약하였으며 연체류중 패각만 채집된 ()에 있어서도 각각 춘도에서 16종, 당사에서는 (0로 커다란 차이를 나타내었다. 온산만에 대한 이러한 저서동물 군집결과는 이 주변의 비철금속 및 석유화학 공업단지로부터 ()입된 중금속으로 인하여 해수 및 생물농축량이 누적됨으로써 나타나는 중금속의 높은 농도와 관련이 있을 것으로 추측된다. The qualitative and quantitative analyses of macrozoobenthic communities sampled from Onsan bay of the eastern coast of Korea were conducted, in this study, to investigate the status of marine pollution. In each survey site, judging from the results of the combined κ-dominance curve for species biomass and individual numbers, it may be assessed that Chundo island, located in innerside of Onsan bay, was in the state of polluted condition both in the summar and winter seasons; on the other hand, Tangsa, the control site of this study, was in the state of unpolluted condition. The species composition of the Chundo island, in addition, was rather lower than that of the Tangsa in richness and showed critical differences with the control site in the number of molluscan species collected as dead shell(16 spp. from Chundo island; 2spp. from Tangsa respectively) It is inferred that the resultant macrozoobenthic community structure of the Onsan bay was related to the high concentration of heavy metals in seawater and bioaccumulation, attributed to the influx of the heavy metals from the surroundings, the nonferrous and the petrochemical industrial complex.

우리나라 老年層의 衣服色嗜好에 관한 硏究

朴準慶,姜順熙 漢陽大學校 韓國生活科學硏究所 1987 韓國 生活 科學 硏究 Vol.- No.5

According as our society has taken rapid development in material civilization and urbanization, the clothes has gained the recognition of its own considerable value in the aspect of social psychology. Though the old aged are inclined to increase every year, there is none the study of their clothes. Therefore, there is necessity for study about the adequate clothing habits suitable to the personality of the old aged on the basis of the change of the society. I investigated the like and dislike and the classiffied preference by seasons for colors of the old aged by sexes, ages and the degrees of school career focusing on this point. The method of the study consisted of the following procedures. First, I made a color chart based on 56 colors which was chosen among 98 colors which categorized by Japanese Color Institute. Second, I made the question papers, and then had a personal interview with the 228 peoples whose age ranges were over 50 years old.

3차원 비디오 자동 동작분석 시스템 개발

박성순,양창수,장준원,이경일,우철호,백승국 한국운동역학회 1998 한국운동역학회지 Vol.8 No.2

The purpose of this study was to develope both hardware and software of automatic 3-dimensional video motion analysis system. In this image capture system, the vedeo image was stored as a file in CD-Rom directly, the refore it was possible to analyze motion without VCR. Reflective markers were used to digitize automaticaly. C-language and MS-Visual Basic 5.0 ] anguage were utiliged in this software to analyze motion. The conclusions were as follows; 1. The vedeo information at sixty frames per second could be captured accurately as a image with a resolution photography of 640×480-24 bit. 2. It was possible to digitize both manually and automatically utilizing reflective marks attached the body. 3. The automatic 3-dimensional video motion analysis system, which was developed in this study not only increased accuracy but also reduced digitizing time so much.

NIHSS 활용을 통한 뇌졸중 환자 간호의 질향상 전략

박경현,송경애,공혜성,나화주,유수경,박소영,김상희,최혜림,배희준,한문구,양미화 한국의료QA학회 2009 한국의료질향상학회지 Vol.15 No.2

문제: 뇌졸중 환자를 돌보는 간호현장에서는 급성기 뇌졸중 환자의 증상악화에 대한 조기사정과 적절한 대처가 매우 중요한 문제이다. 그러나 임상현장에서는 이를 객관화하고 수치화하여 모든 의료진이 의사소통할 수 있는 유용한 도구를 사용하고 있지는 못하는 실정이다. 이러한 문제를 해결하기위해 NIHSS(National Institute of Health Stroke Scale)를 신경과 진료의사를 중심으로활용하고 있지만 아직 간호현장에서는보편적으로사용하지 않고있다. 이에 뇌졸중환자간호에 NIHSS를 활용하여 뇌졸중 증상악화의 조기사정과 빠른 대처로 궁극적으로는 뇌졸중환자 간호의 질을보다 향상시키기 위하여 본활동이 시도되었다. 목적: 첫째, 간호사의 NIHSS 측정 신뢰도의 현수준을 점검하고 이를향상시키기 위한 프로그램을 개발·적용한다. 둘째, 간호사가급성기 뇌졸중환자에게 NIHSS 측정을 통하여 뇌졸중악화를 얼마나 예측할 수 있는지 분석하고 이를 향상시킬수있는 방법을 모색한다. 셋째, 궁극적으로 급성기 뇌졸중 환자의 증상악화의 조기 발견과치료를위한의료진의 올바른 대처지침을 마련하여 이를 적용한다. 의료기관: 경기도에 소재한 대학병원의 뇌졸중 집중치료실 질 향상 활동: 첫째, 간호사의 NIHSS 측정 신뢰도 향상을 위해 「NIHSS 간호사 교육 프로그램」을 마련하였다.

敎授業績評價指標에 관한 實證的 小考

朴魯慶,林俊亨 조선대학교 경영경제연구소 1996 經營經濟硏究 = Management and economics research Vol.19 No.2

ASEAN經濟統合의 效果에 관한 實證的 硏究(Ⅰ)

朴魯慶,金浩中,林俊亨 조선대학교 경영경제연구소 1997 經營經濟硏究 = Management and economics research Vol.20 No.1