석류씨 추출물의 항산화 및 항균활성

고종호,황명오,문주수,황성연,손종연 한국조리과학회 2005 한국식품조리과학회지 Vol.21 No.2

This study was investigated on antioxidative and antimicrobial activities of PSW(pomegranate seed water extract), PSE(pomegranale seed ethanol extract) and PSO(pomegranate seed oil). 'rile extraction yields of PSW, PSE and PSO were 28,9, 13.0 and 4.9%, respectively. Total phenol contents of PSW, PSE and PSO were 47, 78 mg/g(dry basis) and 40 mg/g, respectively. Electron donating abilities of PSW, PSE and PSO at 1,000 ppm were 18.8, 28.5 and 9.7%, respectively. Antioxidative activities in linoleic acid substrates at 500 ppm were in order of 1'SE > a-tocophcrol > PSW > PSO. Antioxidative activities in linoleic acid emulsion substrates at 200 ppm were in order of α-tocopberol > PSE > PSW > PSO, In antimicrobial activity, PSO showed growth inhibition effect against Micrococcus luteus, Salmonella enteritidis and PSW showed growth inhibition effect against Bacillus cereus, Escherichia coll. Whereas antimicrobial activity' of PSE was not observed. The nitrite-scavenging abilities of PSW, PSE and PSO at 2,000 ppm were 27.5, 23.7 and 39.6%, respectively. And the SOD-like activities of PSW, PSE and PSO at 1,000 ppm were 15.9, 34.9 and 0.10%, respectively.

핵산의 절단을 유발하는 전이금속착화합물의 합성

김희정,이범규,고문주 조선대학교 환경연구소 2000 環境硏究 Vol.16 No.1

Study on the synthesis of chemical nucleases that have ability of cleavage of RNA or DNA to investigate the structure of nucleic acids was performed. We synthesized Ni-complex that was synthesized by Karn and Busch (1966) and identified the specificity and ability as chemical nuclease by Chen et. al. (1993), and Cu-complex and Cd-complex that were presumed as having specificity similar to Ni-complex in terms of the structure and specificity and ability as chemical nuclease. The spectra of synthesized new transition metal complexes were compared with those of Ni-complex. Results of comparing data of UV and IR spectra of Cu-complex and Cd-complex with those of Ni-complex showed that the structures of newly synthesized transition metal complexes were similar to the structure of Ni-complex. The yield of synthesis of Ni-complex, Cu-complex, and Cd-complex was 81%, 7%, and 39% respectively.

핵산의 절단을 유발하는 전이금속착화합물의 합성

김희정,이범규,고문주 조선대학교 환경공해연구소 2000 環境公害硏究 Vol.16 No.1

Study on the synthesis of chemical nucleases that have ability of cleavage of RNA or DNA to investigate the structure of nucleic acids was performed. We synthesized Ni-complex that was synthesized by Karn and Busch (1966) and identified the specificity and ability as chemical nuclease by Chen. et. al. (1993), and Cu-complex and Cd-complex that were presumed as having specificity similar to Ni-complex in terms of the structure and specificity and ability as chemical nuclease. The spectra of synthesized new transition metal complexes were compared with those of Ni-complex. Results of comparing data of UV and IR spectra of Cu-complex and Cd-complex with those of Ni-complex showed that the structures of newly synthesized transition metal complexes were similar to the structure of Ni-complex. The yield of synthesis of Ni-complex, Cu-complex, and Cd-complex was 81%, 7%, and 39% respectively.

전신성 홍반성 루푸스에서 F-18 FDG PET상 기저핵 포도당대사 증가 소견을 보이는 무도병 1예 : Evidence for Bilateral Putaminal Hypermetabolism on F-18 FDG PET

서욱장,정선미,고수진,이창근,김재승,임주혁,유빈,문희범 대한핵의학회 2003 핵의학 분자영상 Vol.37 No.5

Purpose: We describe a 54-year-old woman with systemic lupus erythematosus (SLE) who suddenly presented with chorea and had positive antiphospholipid antibodies. F-18 FDG PET showed abnormally increased glucose metabolism in bilateral putamen and primary motor cotex. Tc-99m ECD SPECt also showed abnormally increased regional cerebral blood flow in bilateral putamen. She was treated with corticosteroid and aspirin after which the symptoms improved. Four months later, follow up F-18 FDG PET showed improvement with resolution of hypermetabolism in bilateral putamen. This case suggests that striatal hypermetabolism is associated with chorea in SLE.

병원근무자에 대한 특수건강진단 적용 가능성에 관한 연구

이수일,조병만,조봉수,김영욱,고광욱,문남출,김주원 大韓産業醫學會 1996 대한직업환경의학회지 Vol.8 No.2

병원근무자들에 대한 특수건강진단 적용 여부를 판단하기 위한 기초자료를 제공하기 위해 1995년 3월 10일부터 4주간에 걸쳐 부산시내에 위치하는 2개 종합병원 근무자 830명을 대상으로 설문조사를 시행해 얻은 결과를 요약하면 다음과 같다. 1. 조사된 43개 항목 중 10% 이상의 대상자가 유해요인에 폭로되고 있다고 응답한 항목은 소음, 분진 등 16개 항목이었다. 이 중 분진, 소음 및 스트레스는 연구대상자의 50%이상에서 폭로되고 있다고 응답했으며 그 외 알콜류 소독제(41.3%), 방사선(34.6%), 일반약제(33.3%)의 순으로 폭로되고 있었고 VDT의 경우 25.2%, aldehyde류는 18.7%에서 폭로되고 있는 것으로 나타났다. 2. 대상자의 63.1%에서 병원근무 환경 중에 유해인자가 존재한다고 응답했고 병원의 환경측정이 필요하다고 대답한 사람은 80%에 달했다. 35.7%가 유해인자에 대한 교육을 받은 적이 없다고 응답했다. 전체 응답자의 35.7%가 유해인자에 폭로시 보호 장비를 착용하지 않는다고 응답했고 주기적인(격년) 건강진단은 48.6%에서 받는 것으로 조사되었다. 근무부서의 환기상태는 7.2%만이 좋은 편이라고 응답했다. 이상에서 살펴본 바와 같이 병원근무자들이 여러 가지 유해환경에 노출되고 있고 또, 이로 인해 발생할 수 있는 건강장해에 대한 예방대책이 소홀한 것으로 나타나 병원의 작업환경측정의 시행이 필요하고 이 결과를 바탕으로 병원근무자들에 대한 특수건강진단 적용여부를 결정해야 할 것이다. This study was performed to decide the possibility of special health examination to the hospital employees. We carried out questionnaire survey for 830 subjects at 2 general hospitals located in Pusan city. The summary of this study are as follows. 1. Above 10% of total hospital employees were replied that they exposed to 16 hazard items, such as dust, noise, stress and etc. Among them, over 50% of hospital employees exposed to dust(69.8%), noise(52.3%), stress(60.1%). And hospital employees exposed to alcoholic disinfectant(41.3%), radiation(34.6%), drug(33.3%), VDT(25.2%), and aldehyde(18.7%) also. 2. 63% of the hospital employees replied that there were hazardous factors in hospital environments, and 80% insisted the necessity for hospital environment measuring. 35.7% of respondents replied that they had never taken the education about hazardous factors. 35.7% of the total respondents answered that they didn't use protective equipment when exposed to hazardous factors. And 48.6% answered they took periodic health examination. Only 7.2% replied that ventilation condition of office room was proper. As shown in above results, hospital employees are exposed to various hazards, and turn out neglecting to health problems caused these hazardous factors. So at first, it is necessary to measuring hospital working environment, and then will be decided the possibility of special health examination to the hospital employees.

처분장근처에서 침식에의한 Sr 화학종의 평형

조윤정,김성현,이인화,김시욱,고문주,박성규,이범규 조선대학교 환경연구소 2000 環境硏究 Vol.16 No.1

Leaching and adsorption equilibria of Sr Chemical Species from the near field of a repository has been studied in solidified cement with Fe and oxidized Fe. The absorption percent, Ads %, and distribution coefficient, K_d were calculated using equilibrium concentration to predict the extent of leaching and adsorption. In the mixed aqueous solutions the Ads % increase as Cement(C)/Water(W) ratios increase and K_d was decreased for Sr. These behaviors were discussed by adsorption and equilibrium of spiked ions. Oxides Fe promoted adsorption of spiked ions, but pure Fe had no effect. E_h values were also estimated and discussed to elucidate oxidation-reduction environment of experimental system.

처분장근처에서 침식에 의한 Sr 화학종의 평형

조윤정,김성현,이인화,김시욱,고문주,박성규,이범규 조선대학교 환경공해연구소 2000 環境公害硏究 Vol.16 No.1

Leaching and adsorption equilibria of Sr Chemical Species from the near field of a repository has been studied in solidified cement with Fe and oxidized Fe. The absorption percent, Ads %, and distribution coefficient, K_(d) were calculated using equilibrium concentration to predict the extent of leaching and adsorption. In the mixed aqueous solutions the Ads % increase as Cement(C)/Water(W) ratios increase and K_(d) was decreased for Sr. These behaviors were discussed by adsorption and equilibrium of spiked ions. Oxides Fe promoted the adsorption of spiked ions, but pure Fe had no effect. E_(h) values were also estimated and discussed to elucidate oxidation-reduction environment of experimental system.

Properties of Electron Carriers in the Process of Methanol Oxidation in a New Restricted Facultative Marine Methylotrophic Bacterium, Methylophaga sp. MP

( Moon Joo Koh ),( Chun Sung Klm ),( Yun A Klm ),( Hack Sun Choi ),( Eun Hee Cho ),( Eung Bin Kim ),( Young Min Kim ),( Si Wouk Kim ) 한국미생물 · 생명공학회 2002 Journal of microbiology and biotechnology Vol.12 No.3

Methanol dehydrogenase (MDH) and c-type cytochromes from marine methanol-oxidizing bacterium, Methylophaga sp. MP, were purified and characterized. The native MDH had a molecular mass of 148 kDa and its isoelectric point was 5.5. Two c-type cytochromes, C(L) and C(H), were found, and their isoelectric points were 3.4 and 8.0, respectively. The purified MDH had higher thermal stability than that of the other soil methylotrophic bacteria. The electron flow rate from MDH to cytochrome C(L) was higher than that from MDH to cytochrome C(H), indicating that the physiological primary electron acceptor for MDH is cytochrome C(L). The electron transfer from MDH to phenazine ethosulfate (PES, artificial electron acceptor) in the two dye (PES/DCPIP)-linked assay system was not inhibited by NaCl, whereas the electron flow from MDH to cytochrome C(L) in the cytochrome/DCPIP-linked assay system was suppressed significantly by NaCl. Metal chelating agents such as EDTA showed the same effects on the MDH activity.

Assessment of Parenting Attitudes by Children and Adolescents with Migraine

Kyeong Mi Lee(Kyeong Mi Lee),Min Suk Koh(Min Suk Koh),Ho-chan Lim(Ho-chan Lim),Soorack Ryu(Soorack Ryu),Yong Joo Kim(Yong Joo Kim),Jin-Hwa Moon(Jin-Hwa Moon) 대한소아신경학회 2022 대한소아신경학회지 Vol.30 No.4

Purpose: We aimed to investigate the parenting attitudes reported by patients and their relationships with the characteristics of headaches in children and adolescents with migraine. Methods: We conducted a retrospective review of medical records of children and adolescents with migrainous headaches (n=115; 59.1% female; mean age, 11.89±2.00 years). Children evaluated parental attitudes using the Parenting Attitude Test-Youth (PAT-Y), which comprises eight subscales and four newly devised secondary subscales. Headache severity was calculated by the visual analog scale (VAS), monthly frequency (MF), and VAS×MF/4 (VF). The scores of PAT-Y subscales and the correlations between PAT-Y scores and headache severity were analyzed by age group and sex. Scores for children’s depression inventory, childhood behavior checklists, and an attention deficit hyperactivity disorder scale were also analyzed. Results: In the elementary school age group, VAS was weakly negatively correlated with the “achievement press” (r=-0.28, P<0.05) and “high expectation” (r=-0.25, P<0.05) attitudes, and VF was weakly negatively correlated with “achievement press” (r=-0.32, P<0.05), “punishment” (r=-0.27, P<0.05), and “high expectation” (r=-0.29, P<0.05). In the middle-school age group, MF and VF were moderately positively correlated with the “achievement press” attitude (r=0.48, P<0.01 and r=0.48, P<0.01, respectively), VF was weakly positively correlated with the “neglectful” attitude(r=0.31, P<0.05), and MF was weakly positively correlated with scores for depression (r=0.29, P<0.05) and internalized problems (r=0.31, P<0.05). Conclusion: Parenting attitudes perceived by children and adolescents with migrainous headaches varied by age, and some parenting attitudes were related to headache severity. Education on age-appropriate parenting attitudes may help cope with migrainous headaches.

고식적 위절제술을 받은 진행성 위암환자에서의 5-Fluorouracil, Adriamycin 및 Mitomycin(FAM) 3 제병용 화학용법의 효과

고은희,김주항,박인서,문영명,김병로,김병수,최홍재,민진석 대한내과학회 1986 대한내과학회지 Vol.30 No.3

This study compared the survival of 41 patients who were treated with palliative resection followed by combination chemotherapy with 5-fluorouracil, adriamycin and mitomycin(FAM) to 31 patients treated only by palliative resection for advanced gastric carcinoma. The patients were serially collected from March 1982 to December 1984. The survival rate at 1 year was significantly higher in FAM treated group (FAM treated group, 63.6%, control group, 37,5%; p$lt;0. 05), thereafter, patients treated with FAM, have shown continued tumor progression and death, and there was no significant survival difference at 2 nd year. Two year survival rate of 38 patients with stage IV treated with FAN was 31.4go, on the contrary, in control group, none of 25 patients survived at 2 year after resection(p$lt;0.05). The median survival of FAM treated group was 12.3 months, and 10. 3 months in control group. The survival duration of stage IV, in FAM treated group(median, 13 months) was significantly longer than control group(median, 8.5 months). Acturial two year survival rate of the patients with microscopic residual disease, showed no significant difference between two treatment groups, However, at 2 years after resection, the patients with gross residual tumor, 32.0% in FAM treated group and none of control group survived(p$lt;0. 05) The median survival period of the patients with microscopic residual tumor was 16 months in control group, of whom three of 12 patients were alive and 11 months in FAM treated group. But, in the patients with gross residual tumor, increased survival duration was observed in FAM treated group (median, 14. 1 months) than in control group(median, 7.3 months). In conclusion, FAM treatment significantly increased the survival period in patients with gross residual tumor and in stage g gastric cancer. But, some other forms of combined modality in addition with FAM would be needed for the further improvement in survival after palliative resection for advanced gestric carcinoma.