가압가열 및 microwave에 의한 중력분 반죽 gliadin의 항원성 변화

곽지희(Ji-Hee Kwak),김꽃봉우리(Koth-Bong-Woo-Ri Kim),이청조(Chung-Jo Lee),김민지(Min-Ji Kim),김동현(Dong-Hyun Kim),선우찬(Chan Sunwoo),정슬아(Seul-A Jung),김현지(Hyun-Jee Kim),최정수(Jung-Su Choi),김성원(Seong-Won Kim),안동현(Dong-Hyun 한국식품과학회 2012 한국식품과학회지 Vol.44 No.1

본 연구에서는 가압가열 및 microwave 처리에 의한 중력분 반죽 추출물 내의 gliadin 단백질의 항원성 변화에 대해 살펴보았다. 중력분 반죽에 가압가열과 microwave를 단독 또는 병행으로 처리하여 ci-ELISA, SDS-PAGE 및 immunoblotting을 실시하였으며, 가압가열 처리에 의해서 anti-gliadin IgG 항체와 gliadin과의 결합력이 다소 감소한 것을 확인하였다. 특히 30 min 이상 처리시 더욱 감소한 것으로 나타났으며, SDS-PAGE와 immunoblotting 결과에서도 gliadin band의 강도가 약해지고 항체와의 반응도 나타나지 않았다. Microwave 처리의 경우, 5 min 이상 처리시 일부 gliadin 단백질의 소실이 관찰되었으나, 항원성에는 큰 변화가 없었다. 또한 가압가열 및 microwave 병행 처리에 의해 항원-항체 결합력이 더욱 감소되었으며, 특히 가압가열 50 min, microwave 10 min 처리시 약 35.0%로 감소되었다. 이상의 결과를 통해 가압가열 처리에 의해 중력분 반죽 추출물 내 gliadin의 항원성이 감소되는 것을 확인하였으며, microwave와 병행 처리하는 경우, 더욱 감소하는 것을 확인하였다. The aim of this study was to determine the optimal physical treatment to reduce the antigenicity of gliadin in wheat dough. Medium wheat dough was treated with an autoclave (5, 10, 30, and 50 min at 121℃, 1 atm), a microwave (1, 5, and 10 min) or both (10, 30, and 50 min/5, 10 min). The proteins in the dough extracts were analyzed by SDS-PAGE and the binding ability of anti-gliadin IgG to gliadin was examined by ci-ELISA and immunoblotting. Results showed that the ability of anti-gliadin IgG to bind to gliadin in wheat dough treated with an autoclave alone or in combination with a microwave was decreased. Especially, it declined to ~77% after autoclaving for 30 min and 35% after both autoclaving for 50 min and microwaving for 5 min. In addition, the intensity of gliadin bands in SDS-PAGE were weakened and anti-gliadin IgG did not recognize gliadin in immunoblotting. However, microwaving alone did not affect the antigenicity of gliadin in wheat dough. These results indicate that autoclaving may affect the reduction of the antigenicity of gliadin in medium wheat dough. Moreover, autoclaving in combination with microwaving is more effective for reducing the antigenicity of wheat dough.

Effect of Forging Type on the Deformation Heterogeneities in Multi‑Axial Diagonal Forged AA1100

Min‑Seong Kim,Sang‑Chul Kwon,Sun‑Tae Kim,Seong Lee,Hyo‑Tae Jeong,Shi‑Hoon Choi 대한금속·재료학회 2019 METALS AND MATERIALS International Vol.25 No.3

The present study investigated the effects that different types of forging exert on the deformation heterogeneities developedin AA1100 during multi-axial diagonal forging. To measure the deformation heterogeneities of deformed workpieces, thevalues for hardness and Kernel average misorientation were measured at the center section following each forging process. Type-D forging that consists of diagonal forging and return-diagonal forging was relatively advantageous compared withType-P forging that includes plane forging and return-plane forging for minimizing the non-uniformity of deformationdeveloped in workpieces. The effective strain developed in a workpiece during the 2 types of forging was simulated using3-D FEA. FEA revealed that the positions and degrees of occurrence for soft and hard-zones in workpieces vary greatlydepending on the forging type. Type-D forging was relatively advantageous compared with Type-P forging for minimizingthe non-uniformity of effective strain developed in workpieces.

Purification and Characterization of Helicobacter pylori ${\gamma}$-Glutamyltranspeptidase

Song, Jae-Young,Choi, Yeo-Jeong,Kim, Jeong-Min,Kim, Yoo-Ree,Jo, Jin-Seong,Park, Jin-Sik,Park, Hee-Jin,Song, Yun-Gyu,Lee, Kon-Ho,Kang, Hyung-Lyun,Baik, Seung-Chul,Youn, Hee-Shang,Cho, Myung-Je,Rhee, Kw The Korean Society for Microbiology 2011 Journal of Bacteriology and Virology Vol.41 No.4

Gamma-glutamyltranspeptidase (GGT) was purified to electrophoretic homogeneity from the cell extract of H. pylori. The purified enzyme consisted of heavy and light subunits with molecular weights of 38 kDa and 21 kDa, respectively. N-terminal amino acid sequence of heavy and light subunits revealed that H. pylori GGT was processed into 3 parts for a signal peptide of 27 amino acid residues, a heavy subunit of 352 residues, and a light subunit of 188 residues during translation. The reaction rate for hydrolysis of ${\gamma}$-GpNA was 84.4 ${\mu}mol/min$ per milligram of protein, and that for the ${\gamma}$-glutamyl transfer from ${\gamma}$-GpNA to gly-gly was 23.8 ${\mu}mol/min$ per milligram of protein. The apparent Km values of H. pylori GGT for ${\gamma}$-glutamyl compounds were on the order of $10^{-3}$ to $10^{-4}$ M and those for acceptor peptides and amino acids were on the order of $10^{-1}$ to $10^{-2}$ M. The GGT protein kept approximately 80% of the initial enzymatic activity on incubation at $60^{\circ}C$ for 15 min. The optimum temperature and pH for reactions of both hydrolysis and transpeptidation were $40^{\circ}C$ and 9.0, respectively. The transpeptidation and hydrolysis reactions catalyzed by H. pylori GGT were strongly inhibited by L-Gln and moderately inhibited by L-Ala, L-Ser, ${\beta}$-chloro-L-Ala, and L-Glu. These results demonstrated that the biochemical properties of H. pylori GGT are different from those of other bacterial GGTs. Further, H. pylori GGT might degrade glutathione in the gastric mucous layer of humans if the enzyme could be secreted in the bacterial niches.

가압가열 및 Microwave 처리에 의한 중력분 Gliadin의 항원성 변화

곽지희(Ji-Hee Kwak),김꽃봉우리(Koth-Bong-Woo-Ri Kim),이청조(Chung-Jo Lee),김민지(Min-Ji Kim),김동현(Dong-Hyun Kim),선우찬(Chan Sunwoo),정슬아(Seul-A Jung),강주연(Ju-Youn Kang),김현지(Hyun-Jee Kim),최정수(Jung-Su Choi),김성원(Seong-Won 한국식품영양과학회 2011 한국식품영양과학회지 Vol.40 No.10

본 연구에서는 가압가열 및 microwave 처리가 gliadin의 항원성에 미치는 영향을 살펴보기 위해 중력분에 가압가열과 microwave를 단독 또는 병행으로 처리하여 Ci-ELISA, SDS-PAGE 및 immunoblotting을 실시하였다. 가압가열 처리의 경우, 처리 시간이 길어질수록 IgG와의 결합력이 감소하였으며, 특히 50분 처리구에서 약 69%로 가장 낮은 결합력을 보였다. 또한 SDS-PAGE와 immunoblotting 결과에서도 무처리구에서 강하게 보였던 gliadin band가 처리에 의해 거의 소실되고 항체와 반응하지 않았다. 가압가열 및 microwave를 병행 처리한 경우도 마찬가지로 gliadin의 결합력이 다소 감소하였으며, 처리구 중에서는 가압가열 50분, microwave 5분 처리구에서 약 73%로 가장 낮은 결합력을 보였다. 반면 microwave를 단독으로 처리하였을 때에는 일부 단백질의 변화는 관찰되었으나 항원성 감소에는 큰 영향을 미치지 않았다. 이상의 결과를 통해 가압가열을 단독 처리에 의해 gliadin의 항원성이 다소 감소되었으며, microwave 병행 처리에 의한 차이는 크게 나타나지 않은 것을 확인하였다. This study was conducted to evaluate the effect of physical treatments on the antigenicity of gliadin in medium wheat flour. The wheat flour was treated with an autoclave (5, 10, 30, 50 min), a microwave (1, 5, 10 min), and both (10, 30, 50 min/ 5, 10 min), and investigated by SDS-PAGE, immunoblotting and Ci-ELISA using anti-gliadin IgG. The results showed that the binding ability of anti-gliadin IgG to gliadin in wheat flour was slightly decreased when autoclaved or when autoclaved and microwaved. Especially, it was reduced to about 69% after autoclaving for 50 min and 73% after autoclaving for 50 min and microwaving for 5 min. In addition, gliadin bands in the 50 min autoclaved group disappeared in both SDS-PAGE and immunoblotting. On the other hand, the antigenicity of gliadin was unaffected by microwaving alone. Consequently, there were no considerable changes in using an autoclave alone or in combination with a microwave. These results suggest that autoclaving may affect the reduction of the antigenicity of gliadin in medium wheat flour.

산성강하물의 침착현상에 관한 연구

최금찬,성낙창,김장호,서정민,황성욱 동아대학교 환경문제연구소 1997 硏究報告 Vol.20 No.1

We get the conclusion of the ionic components of sample and the concentration of metal elements which is collected for acid composition substances using Dry Deposion, Dust Jar method at five points in Pusan. It appears higher ionic concentration from the last of February to the middle of May. It appears higher ionic concentration according to the increasing of collecting period in cation. It is higher ionic concentration according to the passing of the time at all sites in anion. The sum of cation equivalent concentration is higher than the sum of anion equivalent concentration in comparing ionic equivalent concentration. The equivalent concentration of SO₄^(2-), NO^(3-) that is gussed acid density is predominient in anion.

紡絲工程에서의 Emulsion Fume 處理를 위한 充塡塔 適用에 관한 硏究

최동훈,박진식,장성호,김일배,서정민,김장호 동아대학교 환경문제연구소 1996 硏究報告 Vol.19 No.2

This study was carried out to interpret hydraulic behavior and component analysis of the emulsion fume, laboratory work of mass transfer in the packing tower which packed 50mm plastic Hiflow-ring. The results from experiment are as follows : 1. The component of emulsion fume is Cyclododecane, 1-Tridecene, 4-Nonene 5-butyl. The kinds of Alkene contained approxymately 76.23 percent. 2. To compare with conventional packing, 50mm Hiflow-ring could save energy because of low pressure drop under high load. 3. The unique magnituteds of packing witch was used are as follows. Cv = 5.18 × 10^(-4) , m = 0.683, n=0.462 so we can predict efficiency which occurres. 4. To require plant of the packing tower in the gas capacity factor 2.7 (Kg^(1/2) m^(-1/2) s^(1))

혈당검사 방법 별 지각된 통증정도에 대한 비교 : 시각적 상사 척도 이용

최자윤,장금성,김현오,최옥엽,박민희 성인간호학회 2003 성인간호학회지 Vol.15 No.1

Purpose: The purpose of this study is to determine the level of pain related to a blood sugar test. Specifically, the study attempts to compare the varying degree of pains when different types of blood sugar test are used. Method: A sample of 56 subjects is composed of DM patients admitted to a medical ward of C university hospital in Gwangju. Data were collected from July, 2001 to December, 2001. The blood sugar tests were administered in four different ways: (1) the use of 27G needle only. (2) the use of 27G needle followed by ice-packed treatment, (3) the use of 27G needle after EMLA cream application, and (4) the use of lancet. The degree of pain is measured with a visual analogue scale and performed twice. Result: in both measures, the use of 27G needle only method is shown to cause the highest level of pain in comparison with the rest of methods (F=4.01, p=.01; F=8.14, p=.00). However, the differences in pain between time in all methods were not found to be significant (t=-.85, p=.40; t=.80, p=.42; t=.31, p=.75; t=.19, p=.85). Conclusion: The study results indicate that the method using lanceter is more recommendable than the use of 27G needle only method. Further research is needed to support the current study result with the use of different measurement scales and to determine effective methods of blood sugar test to lower pain and compliance.

함입된 상악 중절치의 외과적 재위치 후 예후

민성진,최형준,류정아,김성오,이제호,최병재,손흥규 대한소아치과학회 2006 大韓小兒齒科學會誌 Vol.33 No.3

벤처기업의 지역적 특성에 관한 연구 : 수도권과 지방의 비교·분석을 중심으로

최홍봉,윤성민 한국경제지리학회 2004 한국경제지리학회지 Vol.7 No.1

이 연구는 수도권에 위치한 벤처기업과 지방에 위치한 벤처기업 사이에 어떠한 차이가 존재하는가를 분석하는데 목적이 있다. 중소기업청의 '벤처기업 경영실태조사' 원자료를 이용하여 실증분석한 결과는 다음과 같다. 첫째, 수도권에서는 소프트웨어·정보통신 등 첨단 서비스 업종이나 첨단 제조업의 비중이 상대적으로 큼에 비해 지방에는 전통 제조업에 속하는 벤처기업의 비중이 상대적으로 컸다. 둘째, 수도권 벤처기업의 네트워크적 특성은 상대적으로 기업간 기술협력 활동이 활발하고 마케팅 분야에서의 기업간 전략적 제휴에 관심이 많은 반면, 지방의 벤처기업의 경우에는 대학과의 기술협력활동이 활발하고 기술개발활동에 전략적 제휴의 목표가 주어져 있음이 나타났다. 셋째, 수도권에 위치한 벤처기업들이 기술개발능력이 지방의 벤처기업들 보다 더 뛰어났으며 경영성과도 좋은 것으로 나타났다. In this paper, we tries to investigate whether there are some differences of the characteristics between venture business in the metropolitan area and in the local area. We set up some hypotheses on the regional characteristics of venture business and test those using the raw data of Small and Medium Business Administration (SMBA) survey on the management of venture business. Our main findings are as follows: First, we analyze the industrial composition of ventures by region and find that in the metropolitan area high-technology venture business which is belong to software, or information, or communication industries is given much weight. But in the provinces, ventures belong to traditional manufacturing industries is given much weight. Second, we compared the characteristics of business network by region and find that the ventures in the metropolitan area are active in technical cooperation among companies and want strategic tie-ups in marketing field, whereas the ventures in the provinces are active in technical cooperation with university and want strategic tie-ups in technical development field. Third, ventures in the metropolitan area are more excellent in technical development and management than ventures in the provinces.

우리나라 해안에 적합한 표사량 산정식 이론의 선정을 위한 기초적 연구

최민성,김수삼 중앙대학교 생산공학연구소 1993 생산공학연구소 논문집 Vol.2 No.1

해안지역의 이용극대화에 대한 관심이 고조되면서 해안환경공학적인 연구의 측면에서 해안의 침식이나 퇴적의 원인이 되는 표사현상에 관한 정량적인 연구의 필요성이 증대되고 있다. 그러나 우리나라의 경우에는 아직 우리실정에 맞는 표사량산정식이 개발되지 못하고 있다. 그러므로 해외이론을 도입하여 활용하고 있으나 이에 따른 표사량산정식의 적합성에 관한 문제가 발생되고 있다. 따라서 본 연구에서는 우리나라 해안의 특성을 고려하여 대상해안에 적합한 표사량산정식의 선정을 위한 기초적인 지침을 제안하고자 하는 목적으로 최근의 표사량산정식에 관한 이론들을 선정하여 각 이론들의 특성 및 적용한계를 분석하고 이를 검증하기 위해 상주 해수욕장 해빈에 적용하여 수치계산을 통한 적용성을 검토하였다. 수치계산 결과 단기적인 해빈변화를 예측하는 안충표사량산정이론의 경우 Shibayama and Horikawa(1982)의 이론을 제외한 이론들이 실측치와 유사하게 나타나고 있었으며, 장기적인 해안선 변화를 예측하는 연안표사량산정이론의 경우에는 Kraus et al. 이론이 그 적합성이 가장 낮은 것으로 나타나고 있으나 관측된 실측치가 미약하므로 앞으로 장기간의 예측으로 실측치와의 비료를 행하여 관련이론들의 검증이 이루어져야 한다고 사료된다. There has been more interests in the use of the seashore in this days and thus the needs for the quantitative study of the sediment transport phenomenon which causes the beach change have increased. In the case of Korea, the theory of sediment transport rate that fits for condition considering regional characteristics, has not yet developped, thus used the theory from foreign country so that there has been some problems related to its suitability. Hence, this study has carried out that suggest a fundermental guideline for the selection of sedimenttransport rate theory which is suitable for the object beach through the comparison of experimental condition and the analysis of characteristics of related recent sediment transport rate theories. In order to verify the actual possibility of application of related theories, performed the numerical simulation with SANG JU BEACH located in southern seashore of Korea as a object beach. As a result from the numerical simulation with SANG JU BEACH, for the theory of cross-shore sediment transport rate, except for the theory of Shibayama and Horikawa(1982), most theories generally agree with the measurement values, which shows the possibility of application. For the theory of longshore sediment transport rate, the possibility of application of the theory of Kraus et al. which showed great changes in short term seems to be the lowest, so further comparison with the measurement values is required.