C -11 및 F - 18 표지 콜린의 합성과 체내동태에 관한 연구

전권수,유국현,김상욱,임상무,홍성운,서용섭,양승대,안순혁,허민구 대한핵의학회 2001 핵의학 분자영상 Vol.35 No.3

Objectives: Recently, [methyl-(11)^C]-(β-Hydroxyethyl)trimethylammonium ([(11)^C]choline) has been discovered to be a very effective tracer in imaging various human tumors using positron emission tomography. Because of the short half-life of C-11, it is very difficult to use in a routine imaging procedure and needs a frequent synthesis of [(11)^]choline. This can be supplemented by the substitution of [(11)^Ccholine with [methyS-18]fluorocholine. Here, we would like to report cell uptake and biodistribution of [(11)^Ccholine and [(18)^F]fluorocholine as a basic study. Methods [(11)^C]Choline was prepared by the treatment of [(11)^C]CHzI with N,N-dimethylaminoethanol and [18F]fluorocholine was synthesized from reaction of CHzBr[18F]F with N,N-dimethylaminoethanol. The radiochemical purity was checked by high performance liquid chromatography (HPLC). The biodistribution of [(11)^C]choline and [(18)^F]fluorocholine was determined in balb/c mouse at 5 min, 20 min, 40 min and 80 min. The cell uptake wa measured using glioma (9L) and colon adeocarcinoma (SW620). Results: The radiochemical purity was more than 98% after purification. In the liver, uptake did not change over time the uptake was 20/ID/g for [C]choline and 13%ID/g for [(18)^F]fluorocholine. In the kidney, radioactivity decreased over tirne the uptake was 15%1D/g for [(11)^Ccholine and 20%ID/g for [(18)^F]fluorocholine, 80 min post-injection. The cell uptake of [(11)^Ccholine was 4.93% for glioma (9L) and 18.69F for colon adenocarcinoma (SW620). For [(18)^F]fluorocholine, 1.77% for glioma (9L) and 2.77% for colon adenocarcinoma (SW620). Conclusion: [(11)^CCholine and [(18)^F]fluorocholine showed a different cell uptake tendency, depending on cancer cell line. (Korean J Nucl Med 200135:185-191)

분변토를 이용한 VOCs 흡착에 관한 기초연구

김재홍,손희정,김장호,김수생,서정민 동아대학교 환경문제연구소 1997 硏究報告 Vol.20 No.2

This study was carried out two point view that reuse of sludge and adsorption of benzene, toluene and o-xylene of VOCs in cast, carbonized cast and activated carbon. The cation exchange capacity of cast and carbonized cast were 59.2, 112 meq/l00g, respectively. The specific surface were 560, 800 ㎡/g, respectively. The average removal rates of benzene by 50g cast of 50% hydrous cast, anhydrous cast, carbonized cast, activated carbon were 15.0, 41.2, 88.2, 99.4% in 60min retention time. The average removal rates of toluene by 50g cast of 50% hydrous cast, anhydrous cast, carbonized cast, activated carbon were 12.5, 34.2, 88.2, 99.5% in 60min retention time. The average removal rates of o-xylene 50g cast of 50% hydrous cast, anhydrous cast, carbonized cast, activated carbon were 8.8, 28.5, 84.8, 98.1% in 60min retention time. The adsorption efficiency of test absorbent was in order of benzene > toluene > o-xylene.

Ito 저색소증

이근수,장홍준,서연림,안규중,김종민,이종주 ( Geun Soo Lee,Hong Zoon Jang,Yeon Lim Suh,Kyu Joong Ahn,Jong Min Kim,Chong Ju Lee ) 대한피부과학회 1990 대한피부과학회지 Vol.28 No.5

A Case of Hypomelanosis of lto Geun Soo Lee, M.D., Hong Zoon Jang, M.D., Yeon Lim Suh*, M.D., Kyu Joong Ahn, M.Dl., Jong Min Kim, M.D., Chong Ju Lee, M.D. Departments of Dermatology and Pathology*, Clooege of Medicine, Hallym University Seoul, Korea We report a case of hypomelansis of Ito developed in a 6-year-old male patient. He had numberous bizarre, hypopigmented streaky macules on the left side of the chest, and upper arm. The histopathologi findings demonstrated that hypopigmented areas contained slightly decreased basal pigmentation with decreased content of intracellualr melanin in keratinocytes and melanocytes and revealed neither inflammatory changes nor dropping of melanin granule into the dermis. The electron microscopic finding showed decreased number of melanosomes, incomplete melaniztion, and cytoplasmic vacuoles in keratinocytes and melanocytes. Extra-cutaneous alterations were not found.

TiN/TiSi₂-bilayer 형성과 전기적 특성

서경수,황찬용,이광만,최치규,강민성 濟州大學校 基礎科學硏究所 1997 基礎科學硏究 Vol.10 No.2

TiN/TiSi₂-bilayers were formed by reactive sputter deposition in mixed gas of Ar+N₂ on the TiSi₂/Si(100) substrate, which TiSi₂ film was grown by means of the Ti sputtering, and the coevaporation of Ti and Si(Ti : Si = 1 : 2) on the Si(100)-2 ×1 substrate followed by in-situ annealing in ultrahigh vacuum. Stoichiometric Ti??N?? films with (111) texture determined XRD, RBS and XPS were grown at substrate temperature over 600℃, while films prepared at substrate temperature below 600℃ showed N-rich TiN, and the high quality TiSi₂ film was obtaind from the Ti/Si(100) and (Ti+2Si)/Si(100) samples annealed at 700℃. It is capping effect that the TiSi₂ film in the TiN/TiSi₂/Si(100) structure does not occure the agglomeration phenomena. The sheet resistance of the TiN/TiSi₂-bilayer prepared at 700℃ showed 0.39Ω/㎝².

Antinociceptive profiles and mechanisms of orally administered coumarin in mice.

Park, Soo-Hyun,Sim, Yun-Beom,Kang, Yu-Jung,Kim, Sung-Su,Kim, Chea-Ha,Kim, Su-Jin,Lim, Su-Min,Suh, Hong-Won Pharmaceutical Society of Japan 2013 BIOLOGICAL & PHARMACEUTICAL BULLETIN Vol.36 No.6

<P>In the present study, the antinociceptive profiles of coumarin were examined in ICR mice. Coumarin administered orally (from 1 to 10 mg/kg) showed an antinociceptive effect in a dose-dependent manner as measured in the acetic acid-induced writhing test. Duration of antinociceptive action of coumarin maintained at least for 60 min. But, the cumulative response time of nociceptive behaviors induced by a subcutaneous (s.c.) formalin injection, intrathecal (i.t.) substance P (0.7 ?g) or glutamate (20 ?g) injection was not affected by coumarin. In addition, intracerebroventricular (i.c.v.) or intrathecal (i.t.) administration with coumarin (10-40 ?g) attenuated acetic acid-induced writhing response in a dose dependent manner. Intraperitoneal (i.p.) pretreatment with naloxone (an opioid receptor antagonist) attenuated antinociceptive effect induced by coumarin in the writhing test. Furthermore, i.c.v. or i.t. pretreatment with naloxone (5 ?g) reversed the decreased acetic acid-induced writhing response. However, methysergide (a 5-HT serotonergic receptor antagonist) or yohimbine (an α2-adrenergic receptor antagonist) did not affect antinociception induced by coumarin in the writhing test. Our results suggest that coumarin exerts a selective antinociceptive property in the acetic acid-induced visceral-derived pain model. Furthermore, the antinociceptive effect of coumarin may be mediated by activation of central opioid receptors, but not serotonergic and adrenergic receptors.</P>

조증에서 Carbamazepine과 Lithium Carbonate의 치료적 효용성의 비교

이민수,서광윤,이병윤 大韓神經精神醫學會 1987 신경정신의학 Vol.26 No.4

Thirty-five manic patients diagnosed by DSM-Ⅲ criteria participated in the comparision study of the acute antimanic effects of the carbamazepine and lithium carbonate, at average dose of 1100mg/day, achieving mean ±SD blood level of 7.93±0,49㎍/ml (range 0.65-13.09㎍/ml), and 1720㎎/day, 1.20±0.25mEq/L(range 0.5-1.55 mEq/L), respectively. Both drugs effectively reduced manic symptomatology. Sixty-five percent in carbamazepine group (N=20) showed moderate to marked improvement, and 53 percent in lithium carbonate group (N=15) in clinical global improvement. From the 3rd day, carbamazepine group showed statistical significance in both brief psychiatric rating scale and manic state rating scale scores (P<0.0005, P<0.005), on the other hand 7th day in lithium carbonate group (P<0.025, P<0.01), While baseline brief psychiatric rating scale and manic state rating scale scores were not virtually different, the differences between two groups at 10th day approached statistical significance (P<0.05, P<0.05). There was no significant correlation between blood level and clinical improvement as indicated by percent amelioration in both groups after 4 weeks of treatment. There was no significant side effect except 3 cases of drop-out in the carbamazepine group. This data suggest that carbamazepine might be a useful alternative drug for the treatment of mania.

간질환자의 출생 순위에 관한 임상적 연구

이민수,조숙행,한선호,서광윤,이병윤 大韓神經精神醫學會 1981 신경정신의학 Vol.20 No.2

To provide data on the clinical issues related to the birth order and other characteristic background in epilepsy, 331 idiopathic epileptic patients were evaluated and analyzed phenomenologically. The patients had been treated at the neuropsychiatric department of the Korea University Hospital during 1974 to 1980. The age at onset ranged from 1 to 45 years, with the mean age of 16.65 years. Males were 1.36 times more than females. Seventeen (5.13%) of the subjects had the family history of epilepsy. Attacks in the daytime only occurred in 67.67% (N=224) of the subjects, while 23.56% (N=78) had their seizures only at night, and 8.77% (N=20) had their seizures both at daytime and night. Grand mal was the most frequent type of seizure, followed by psychometer and petit mal. There was a higher incidence among the first born than the subsequent siblings and the children born second had a somewhat higher frequency of epilepsy than the subsequent children.

양파의 껍질 및 육질 추출물이 CCl₄투여 흰쥐의 지질과산화적 손상에 미치는 영향

이재민,정수현,조정순,서형주,정승태 明知大學校 自然科學硏究所 1997 자연과학논문집 Vol.15 No.-

CCl₄투여한 흰쥐에서 양파껍질과 양파육질 추출물의 항산화 효과를 알아보기 위해 SOD활성도 측정을 이용한 유리 라디칼 소거능, 과산화지질(Malondialdehyde, MDA),GOT, GTP를 측정한 결과 CCl₄ 투여로 증가된 IC 값(cytochrome C 환원의 50%의 저해 함량) 은 양파껍질과 육질 추출물에 의해 유의하게 감소되었다. MDA함량은 양파껍질 추출물에 의해 그 함량이 유의하게 감소되었다. 또한 혈청내 GOT와 GTP의 함량도 역시 양파껍질과 육질추출물에 의해 감소되었다. Using the CCl₄-induced hepatotxicity in rats as model system, superoxide radical scavenging activities by SOD(Super oxide dismutase), TBA-reactants(MDA) contents, GOT acd GPT were measured for antiaxidative activity in onion peel and onion flesh. The results were summarized as follows, IC (Inhibition concentration of 50% cytochrom Creduction)values which were increased with intra peritoneal injection of CCl₄were siginificantly decreased by the extracts from onion peel and onion flesh. The contents of MDA(malon dialdehyde) in liver were increased CCl₄ injection whereas the contents were siginificantly decreaed with the extracts from onion peel and were slightly decreased with the extracts from onion flesh. The contents of GOT and GPT in serum were also decreased with the extracts from onion flesh. The contents of GOT and GPT in serum were also decreased with the extracts from onion peel and onion flesh.

Hop Extract Produces Antinociception by Acting on Opioid System in Mice

Park, Soo-Hyun,Sim, Yun-Beom,Kang, Yu-Jung,Kim, Sung-Su,Kim, Chea-Ha,Kim, Su-Jin,Seo, Jee-Young,Lim, Su-Min,Suh, Hong-Won The Korean Society of Pharmacology 2012 The Korean Journal of Physiology & Pharmacology Vol.16 No.3

In the present study, the antinociceptive profiles of hop extract were characterized in ICR mice. Hop extract administered orally (from 25 to 100 mg/kg) showed an antinociceptive effect in a dose-dependent manner as measured in the acetic acid-induced writhing test. Antinociceptive action of hop extract was maintained at least for 60 min. Moreover, cumulative response time of nociceptive behaviors induced with intraplantar formalin injection was reduced by hop extract treatment during the 2nd phases. Furthermore, the cumulative nociceptive response time for intrathecal injection of substance P ($0.7{\mu}g$) or glutamate ($20{\mu}g$) was diminished by hop extract. Intraperitoneal pretreatment with naloxone (an opioid receptor antagonist) attenuated antinociceptive effect induced by hop extract in the writhing test. However, methysergide (a 5-HT serotonergic receptor antagonist) or yohimbine (an ${\alpha}_2$-adrenergic receptor antagonist) did not affect antinociception induced by hop extract in the writhing test. Our results suggest that hop extract shows an antinociceptive property in various pain models. Furthermore, the antinociceptive effect of hop extract may be mediated by opioidergic receptors, but not serotonergic and ${\alpha}_2$-adrenergic receptors.

154kV용 활선 안전 경보기 개발에 관한 연구

정민규,조현광,우종수,안창회,서영석,김응태,박창기,오세득 영남대학교 공업기술연구소 2000 工業技術硏究所論文集 Vol.28 No.1

In this report we developed a high voltage alarm for the safety of workers who check and repair the 154kV power line. This high voltage alarm we developed is attached on workers' helmet and alarms automatically in order to let workers know when workers work over the safety distance near 154kV power line. Until now the exact safety distance hasn't been regulated and high voltage alarm we've been using was for the range of 6∼22.9kV power line. We fabricated this high voltage alarm on the basis of electric density and actual measurement of power line. The experimental result was that high voltage alarm at 66kV power line alarmed near 90cm, at 154kV power line alarmed near 160cm from the line. Accordingly, we developed the new high voltage alarm which is operated in the range of 66∼154kV power line.