열처리에 의한 Morganella morganii와 Photobacterium phosphoreum 유래 Histidine Decarboxylase의 불활성화

박원민(Won-Min Pak),김꽃봉우리(Koth-Bong-Woo-Ri Kim),김민지(Min-Ji Kim),박지혜(Ji-Hye Park),배난영(Nan-Young Bae),박선희(Sun-Hee Park),안동현(Dong-Hyun Ahn) 한국식품영양과학회 2016 한국식품영양과학회지 Vol.45 No.3

Histamine을 생성하는 Morganella morganii와 Photobacterium phosphoreum으로부터 crude histidine decarboxylase(HDC)를 추출하여 65~121°C로 열처리한 다음 균의 생육 및 효소 활성 변화에 대해 살펴보았다. 그 결과 M. morganii와 P. phosphoreum은 모든 열처리 조건에서 비 가열 처리구와 비교 시 균의 생육이 저해됨을 확인하였다. M. morganii와 P. phosphoreum 유래 HDC의 효소 활성은 65°C에서 90% 이상의 효소 활성이 저해됨을 확인하였고, 온도가 증가함에 따라 유의적으로 활성이 억제되는 것으로 나타났다. SDS-PAGE 결과에서는 65~100°C 범위에서 비가열 처리구와 비교 시 조효소액의 단백질 패턴의 변화가 크지 않았으나, 121°C에서 단백질 band의 강도가 크게 약해졌다. Native-PAGE에서는 65°C 처리 조건에서부터 단백질 패턴의 변화가 크게 나타났다. 따라서 가열처리(65~121°C)는 histamine 생성균인 M. morganii와 P. phosphoreum의 생육을 억제할 뿐만 아니라 HDC의 효소 활성도가 저해됨을 확인하여, 식품산업에서 적용되고 있는 열처리 조건에서 histamine 생성 억제에 큰 효과가 있는 것으로 생각한다. This study was performed to investigate the effects of various thermal treatments on the growth of Morganella morganii and Photobacterium phosphoreum and activity of crude histidine decarboxylase (HDC) obtained from M. morganii and P. phosphoreum. Crude HDC and the two strains were treated at 65°C/30 min, 80°C/10 min, 100°C/10 min, and 121°C/10 min. Activity of crude HDC decreased with increasing temperature. Viable cells counts of M. morganii and P. phosphoreum were not detected in any heated samples. SDS-PAGE patterns of heated HDC did not show significant differences up to 100°C. However, at 121°C, protein band intensity was weakened. In native-PAGE, there was a major change in the pattern of HDC at 65°C. These results suggest that thermal treatment can help to reduce histamine production by reducing HDC activity and growth of M. morganii and P. phosphoreum.

Effect of 840 nm light-emitting diode(LED) Irradiation on Monosodium Iodoacetate-Induced Osteoarthritis in Rats

( Hyun-ji Lee ),( Ha-nul Soe ),( Woo-bin Soel ),( Kyung-sun Soe ),( Jong-won Ju ),( Hae-sung Lee ),( Ung-bae Park ),( Min-suk Yu ),( Ji-hyun Lee ) 대한임상병리사협회 2013 조직세포검사학회 발표자료집 Vol.2013 No.-

Background The objective of this study was to evaluate whether 840 nm light-emitting diode (LED) could be effective in a noninvasive, therapeutic device for the treatment of monosodium iodoacetate-induced osteoarthritis in rats. Method Twenty male Sprague-Dawley rats weighing approximately 200 g each were divided into four groups: control group (C); monosodium iodoacetate-treated(MIA) group; monosodium iodoacetate-treated(MIA) group with LED phototherapy(MIA-LED); indomethacin-treated group(IMT). Osteoarthritis(OA) was induced by intra-articular injection of 50 μl of 3 mg MIA through the patellar ligament of the right knee. Control rats were injected with an equivalent volume of saline. In the MIA-LED group, the animal knees were exposed to LED stimulation at intervals of 15 min/day, for 1 weeks after MIA treatment. Animals were sacrificed at 2 weeks postoperatively. Knee joints were removed and fixed overnight in 10% neutral buffered formalin and subsequently decalcified by EDTA for 2 wk before being embedded in paraffin. Frontal sections (4 μm thick) of the medial aspect of the rat knee joints were prepared every 250 μm. The OA clinical score was monitored by knee movement and by radiographic analyses. Histologic analyses were performed following staining with hematoxylin and eosin, Safranin O-fast green, or toluidine blue, and histologic changes were scored according to a modified Mankin system. Results Radiographic examination showed no differences between the MIA-treated and the MIA-LED-treated rats. Sclerotic regions adjacent to the tibioepiphyseal margin, thinning of the epiphysis, and a small exophite were evident in the MIA-treated and the MIA-LED-treated rats, whereas tibioepiphyseal margin was well preserved in the IMT-treated rats compared with the two groups. Histologically, the lesion severity of knee joint by Mankin``s scoring showed not a significant difference between the MIA-treated and the MIA-LED-treated rats, however the lesion score was significantly decreased in the IMT rats compared with the two groups. Discussion The irradiation of LED at 840 nm after monosodium idoacetate treatment was no significant difference between the respective arthritis parameters in LED-treated group compared with indomethacin-treated group. However, the total Mankin score was significantly decreased in MIA-LED-treated group compared with monosodium iodoacetate-treated group. These results indicate that LED phototherapy was some beneficial influence on the repair of chondral lesion in osteoarthritis rats.

중·소형 회계법인의 합병이 감사품질에 미치는 영향

강민지(Kang, Min-JI),배성호(Bae, Seongho) 한국국제회계학회 2021 국제회계연구 Vol.- No.98

[연구목적] 본 연구의 목적은 중·소형 회계법인의 합병이 감사품질에 어떠한 영향을 미치는지 검증하고자 한다. 우리나라는 회계투명성을 높이기 위해 2019년도부터 감사인 등록제를 전격 시행하였으며, 회계법인 시장에서는 이에 대비하여 중·소형 회계법인 간 합병이 빈번하였다. 선행 연구에서는 회계법인의 규모가 클수록 감사품질이 높다는 결과를 제시하고 있으므로 중·소형 회계법인 간 합병도 감사품질을 높이는 역할을 할 것이라 기대된다. 그러나 현재 우리나라의 중·소형 회계법인간 지배구조를 고려했을 때 회계법인 합병은 형식적 요건을 맞추기 위한 수단이며 궁극적으로 감사품질에는 변함이 없을 것이라는 예측도 가능하다. 이에 본 연구에서 이를 실증하고자 한다. [연구방법] 합병 사례는 2019년에 발생한 중·소형 회계법인 간 합병을 대상으로 한다. 2017년부터 2020년의 4개 연도를 표본 기간으로 한 후 2019년 합병을 기준으로 합병 전·후 2년과 합병 전·후 1년을 분석 대상 기간으로 하였다. 합병 회계법인으로부터 회계감사를 받은 경우 피감사회사의 재량적 발생액에 어떠한 영향을 미치는지 분석한다. [연구결과] 실증분석 결과 2019년 기준으로 합병 전·후 2년을 분석 대상으로 하였을 때, 2019년 합병 중·소형 회계법인으로부터 감사를 받은 피감사기업의 재량적 발생액은 5% 수준에서 유의한 음(-)의 값이, 합병 전·후 1년을 분석 대상으로 하였을 때 5% 수준에서 유의한 음(-)의 값이 발견되었다. 이러한 분석 결과를 통해 2019년에 합병한 중·소형 회계법인은 합병 이후 1년, 2년의 감사품질은 증가하는 것으로 나타났다. [연구의 시사점] 본 연구의 결과를 통해 감사품질의 개선 및 향상을 유도하기 위한 감사인 등록제가 긍정적인 효과가 있음을 시사하고 있다. 아울러 회계법인의 규모가 클수록 감사품질이 높다는 선행연구를 재확인시켜주고 있다. [Purpose] This paper aims to examine the relation between merger of small and medium-sized accounting firms and audit quality. In order to increase accounting transparency, Korea implemented the audit registration system in 2019. Accordingly, mergers between small and medium-sized accounting firms occurred frequently in the audit market. In the prior study, there suggest that the larger the accounting firm, the higher the audit quality. Therefore, the merger between small and medium-sized accounting firms is expected to play a role in improving audit quality. However, considering the current governance between small and medium-sized accounting firms in Korea, it can be predicted that the merger of accounting firms can be means to meet the formal requirements of audit registration system, and ultimately the audit quality will not change. [Methodology] The merger sample is for mergers between small and medium-sized accounting firms that occurred in 2019. Four years from 2017 to 2020 were the sample period, Based on the merger in 2019, the two years before and after the merger and one year before and after the merger are compared. This study analyzes the effect on the discretionary accruals of the audited company when it is audited by a merged accounting firm. [Findings] For the two years before and after the merger analysis, the discretionary accruals of companies audited by the merged auditor decreased. Similarly, for the one years before and after the merger analysis, the discretionary accruals also decreased. These results showed that audit quality increased due to the merger of small and medium-sized accounting firms. [Implications] The results of this study suggest that the auditor registration system to induce improvement of audit quality has a positive effect on audit market. In addition, this study reconfirms prior studies that the larger the size of the auditor, the higher the audit quality.

난치성 백반증에 대한 흡입물집을 이용한 비배양 표피세포이식술 20례

배정민 ( Jung Min Bae ),정한미 ( Han Mi Jung ),이한나 ( Han Na Lee ),이로우 ( Ro Woo Lee ),은성혜 ( Sung Hye Eun ),권혁선 ( Hyuck Sun Kwon ),이지혜 ( Ji Hae Lee ),김경문 ( Gyong Moon Kim ) 대한피부과학회 2018 대한피부과학회지 Vol.56 No.7

Background: As nonsurgical interventions for vitiligo are not always successful, various surgical modalities have been used in patients with refractory vitiligo. Of these, non-cultured epidermal suspension transplantation (NCES) was recently introduced to treat large recipient sites using cells from small donor tissue. Objective: We assessed the effectiveness and safety of NCES as a surgical treatment for patients with refractory vitiligo. Methods: We retrospectively reviewed 20 cases in 17 patients (11 females; median age 25 years) who underwent NCES from July 2015 through March 2018. Suction blisters (20 mm in diameter) were collected from the patient’s inner thigh at a donor-to-recipient area ratio of 1:5. After the addition of 5 mL recombinant trypsin solution to the suction blisters, followed by incubation at 37°C for 60 min, epidermal cells were manually scraped off the blister surface, and epidermal cell suspension was obtained by centrifugation at 1,500 RPM for 5 min. The suspension was applied to the vitiligo regions after epidermal ablation of those regions. Phototherapy resumed 1 month later. Treatment success was defined as ≥75% repigmentation of the surgical site, and all adverse events were noted. Results: Overall, 85.0% of cases (17/20) exhibited treatment success. Adverse events included hyperpigmentation (20%) and surgical site infection (5%), but the treatment was tolerable in all cases. Conclusion: NCES is a reliable surgical option for patients with vitiligo refractory to nonsurgical treatment. Large areas of vitiligo can be treated by NCES, and use of this technique should be encouraged in Korea. (Korean J Dermatol 2018;56(7):426∼432)

Exploiting Microwave Chemistry for Activation of Metal-Organic Frameworks

Lee, Eun Ji,Bae, Jinhee,Choi, Kyung Min,Jeong, Nak Cheon American Chemical Society 2019 ACS APPLIED MATERIALS & INTERFACES Vol.11 No.38

<P>Microwave is thought of as a useful electromagnetic radiation tool because it is often used in real life as well as in a variety of chemical processes. Meanwhile, activation of metal-organic frameworks (MOFs), which must be essentially done to remove coordinating and pore-filling solvents before the use of MOFs for various applications, has been performed commonly with the methods of heat supply or solvent exchange. Here, we show a new methodological microwave activation (MA), realizing it with various MOFs such as HKUST-1, UiO-66, and MOF-74s. For instance, microwave irradiation to the MOF samples for 4-35 min leads to the complete activation of the MOFs without structural damage. As described below, we further demonstrate that the solvent-assisted MA, which is the MA process performed after the solvent exchange, can substantially reduce the time for the activation by 4 min.</P> [FIG OMISSION]</BR>

알루미늄 PEO 코팅의 결정상에 미치는 공정 조건에 대한 연구 I. Unipolar 펄스와 코팅시간

김배연,함재호,이득용,김용남,전민석,김기윤,최지원,김성엽,김광엽,Kim, Bae-Yeon,Ham, Jae-Ho,Lee, Deuk Yong,Kim, Yong-Nam,Jeon, Min-Seok,Kim, Kiyoon,Choi, Ji-Won,Kim, Sung Youp,Kim, Kwang Youp 한국결정성장학회 2014 韓國結晶成長學會誌 Vol.24 No.2

전해질로 $Na_2SiO_3$을 사용하여 A1050 알루미늄 판재를 pulse 폭 $ 2000{\mu}sec$, impulse 420 V, 400 ${\mu}$sec의 unipolar pulse로 플라즈마 전해 산화 코팅(plasma electrolytic oxidation coating)을 하여 산화 피막을 2, 5, 15, 30분에 따라 형성시킨 다음 산화피막을 분석하였다. 표면에 형성된 산화물의 결정상은 ${\alpha}-alumina$와 ${\gamma}-Alumina$로서 시간에 따른 변화는 없었다. 반응 초반에는 ${\gamma}-Alumina$가 많이 생성되었지만 시간이 갈수록 ${\gamma}-Alumina$의 양에는 변화 없이 ${\alpha}-alumina$가 많이 생겨남을 알 수 있었다. 이런 결과는 micro plasma에 의해서 ${\gamma}-Alumina$가 우선 생성되고, 이후 계속되는 micro plasma의 열에 의해서 ${\alpha}-alumina$로의 전이가 일어나기 때문으로 판단된다. Crystallographic phases of Plasma electrolytic oxidized Al alloy, A1050, were investigated. The electrolyte of PEO was $Na_2Si_2O_3$ and KOH. Unipolar pulse, $ 2000{\mu}sec$ with $400{\mu}sec+420V$ impulse, were applied for 2 min, 5 min, 15 min, and 30 min. ${\gamma}-Alumina$, as well as ${\alpha}-alumina$, was main crystal phase. ${\gamma}-Alumina$ was appeared in the beginning, then the amount of ${\alpha}-alumina$ was increased with time, but the amount of ${\gamma}-Alumina$ remained constant without any increasing. So, it is concluded that plasma gas produce ${\gamma}-Alumina$ at the first, and then ${\gamma}-Alumina$ transform ${\alpha}-alumina$ finally. During the transformation, high temperature of micro plasma gives transformation energy.

수준별 수영 참여자의 동기유형 분석

배병석,정재은,육동원,김지수,김민현 한국스포츠리서치 2005 한국 스포츠 리서치 Vol.16 No.2

The purpose of this study was to examine motivation type of swimmers by competency level. A total of 262 swimmers were composed of 111 beginners, 58 inter-mediates and 93 advancers participated in the study. For the study, duration of participation, frequency of participation and the level of sport performance of participants were set as the independent variable, while motivation type was the dependent variable. The study used analysis methods such as frequency analysis, t-test, one-way ANOVA between the independent and dependent variables. A significant difference was found after conducting an analysis on the difference in motivation type by duration of participation. The analysis on the difference in motivation type by frequency of participation showed that a group that swam three to five times per week recorded higher marks in terms of physical condition and social life factors than that swam two to three times per week. The analysis on the difference in the motivation type by level of sport performance of participants showed a significant difference in development of skills, enjoyment, achievement, physical condition. social life. external ostentation and amotivation factors.

저 전력 이족 로봇의 계단보행에 관한 연구

배지훈,강민구,박경용,김갑일 명지대학교 산업기술연구소 2001 産業技術硏究所論文集 Vol.20 No.-

In this paper, stair walking of small power biped robot is implemented by load sharing method of walking gait and adding body. Generally, in one foot standing, load is burdened to especial joint. Further, robot's foot is long steps and is lifted up high to go up stairs. So, especial joint is sometimes overloaded during walking and this makes walking impossible. This problem is solved in this paper using adding body, load sharing method and kick action. By this ways, stair walking of small power biped robot can be implemented without overload to joint.

침습성 아스페르길루스증으로 치료받았던 급성 백혈병 환자에서 조혈모세포이식후 발생한 파급성 아스페르길루스증 1례

배기선,박지영,신수연,문영철,최희정,조민선,성주명 대한감염학회 2003 감염과 화학요법 Vol.35 No.4

본 증례는 침습성 아스페르길루스증의 과거력을 갖고 있는 백혈병환자에서 조혈모세포이식을 받은 후 치명적인 파급성 아스페르길루스증이 발병한 예이다. 환자는 항암요법 후 흉부 방사선 및 단층촬영에서 진균성 폐렴을 의심하여 항진균제를 투여하고 폐엽절제술을 시행하여 아스페르길루스에 의한 폐렴임을 확인하였다. Amphotericin B로 치료한 후 조혈모세포이식을 시행받은 뒤 치료에도 불구하고 파급성 아스페르길루스증으로 진행되어 사망하였다. 이 증례에서 보듯이 이전에 아스페르길루스증의 과거력이 있는 경우에 이식후 치명적인 결과를 유발할 수 있으므로, 고위험군을 선별하는 지침과 적절한 치료법에 대한 연구가 필요하겠다. Invasive aspergillosis has been increasing as the number of severe immunocompromised hosts rises. Particularly, in allogeneic hematopoietic stem cell transplantation (HSCT) recipients, incidence of invasive aspergillosis ranges from 4 to 10%. Even with appropriate treatment, the prognosis of invasive aspergillosis in allogeneic HSCT recipients remains poor, showing high mortality rate. Herein. we report a case where invasive aspergillosis in a patient with acute myelogeneous leukemia progressed to disseminated aspergillosis after allogeneic HSCT. A 31-year-old woman with acute myelogenous leukemia had invasive aspergillosis after third reinduction chemotherapy. After administering amphotericin B, the patient underwent the wedge resection of lung. and HLA-matched allogeneic HSCT was then conducted. On day 14 of transplantation, the patient died of disseminated aspergillosis, including possible cerebritis and endocarditis despite the amphotericin B therapy.

초음파 센서를 이용한 이동 로봇

배지훈,김종철,박경용,강민구,김갑일 明知大學校 産業技術硏究所 2000 産業技術硏究所論文集 Vol.19 No.-

In this paper, design and construction of mobile robot is conducted and the error reduction and obstacle avoidance techniques is introduced. Up to now, in the design of most mobile robots, there are emphasis on the compensation of tracking errors using the additional sensors and guidance method, rather than the reduction of the tracking errors of motor itself and wheel errors. In this purpose, high performance motor and encoder are used for the driving actuator, and the acceleration/deceleration technic is introduced to reduce the driving errors. Also ultrasonic sensor performs obstacle avoidance, which is proved using the driving test.