교량 탐사용 로봇 개발

김덕후,김효곤,조국,강민성,박상덕,손웅희 한국공작기계학회 2008 한국공작기계학회 추계학술대회논문집 Vol.2008 No.-

The purpose of maintaining of bridges was to secure of them and expend its life. They wereneeded to have a regular safety check. But, most parts of a bridge were not able to access easily for people so it was difficult to maintain. To improve it, the flight mechanism and mobile mechanism were combined to examine with moving when they were attaching to bridges up and down. The flight mechanism is a co-axial-rotor-helicopter shape that cut the body down and can be done hovering. The mobile mechanism was shaped to move every direction with holding the position of a robot. These robot platforms showed possibilities to use them not only for observation and patrol but they could be used for a new usage with transformation of flying robots. This paper was focused on location maintaining when a robot was examined a bridge. The flight mechanism would only have thrust and adhesive force, the mobile mechanism would be used for moving. Therefore, defining the mechanistic model of the mobile mechanism has proved the validity of the model through simulations and experiments.

수치해석을 이용한 콘크리트의 균열저항곡선 해석

강성후,김민성 東新大學校 工業技術硏究所 1999 工業技術硏究 Vol.5 No.-

Predictions of crack process condition of concrete and fracture parameter have been investigated using the finite element method since concrete fracture mechanics has been introduced. This study is to investigate on the prediction of fracture parameters measured from concrete by applying numerical interpretations based on fracture interpretations of crack process. As the stress from crack tip reached maximum value the crack initiated, and as an unstable crack critical load( P_(IC)) the specimen started to spread and finally to fail. Fracture characteristics values were calculated including effective crack length(a_(e)) and R-curve start point of unstable crack(a_(IC)), which occur due to the working load in nonlinear minute crack of the crack tip.

유사체액에서의 전기화학적 처리가 티타늄 표면의 인산갈슘 흡착에 미치는 영향

조국현,김문영,송광엽,배태성,이민호 大韓齒科器材學會 2002 대한치과재료학회지 Vol.29 No.3

This study was performed to examine the effect of electrochemical treatment on the precipitation of apatite-like calcium phosphate in a simulated body fluid. Titanium plates of 20×20×1 ㎣ in dimensions were polished sequentially from #600 to #2,000 emery paper and one surface of each specimen was received an additional polishing sequence through 0.1㎛ alumina paste. To improve the bioactivity of titanium plates, they were electrochemically treated at 100 mA for 20 min in an electrolyte containing the calcium and phosphate ions, and the Hanks' solution with pH 7.4 at 36.5℃ for 30 days, and the surfaces were examined with X-ray diffractometer(XRD), scanning electron microscope(SEM) and X-ray photoemission spectroscopy(XPS). Precipitation of calcium phosphate and CaO were observed on the electrochemically treated titanium surface in a simulated body fluid after treatment at 600℃. Precipitation of calcium phosphate was densified and coarsened with increasing the temperature of the electrolyte solution in the range of 20-80℃. The calcium phosphate contained phosphate in the form of Po₄^3-, HPO₄^2-, and H₂PO₄^-.

두개내에 발생한 형질세포육아종 (Plasma Cell Granloma) 2예

천대웅,주성민,김후원,김병기,안병철,오건세,양기화 대한신경과학회 1999 대한신경과학회지 Vol.17 No.3

방과후 학교의 운영체제 개선 방안 연구

이승미,김대영,홍후조,민부자 한국교육과정학회 2007 교육과정연구 Vol.25 No.3

치과주조용 Ti-Zr-Cr계 합금에서 Cr 함량변화가 부식저항성에 미치는 영향

박영준,강후원,이용렬,송호준,김민강 大韓齒科器材學會 2004 대한치과재료학회지 Vol.31 No.4

The purpose of this study was to develop new dental casting titanium (Ti) alloys having an excellent mechanical properties and good corrosion resistance as a substitute for c.p Ti and Ti-6Al-4V alloy (control groups). Ti-20Cr, Ti-2OZr and Ti-20Zr-XCr (X=2, 5, 10, 15) alloys (experimental groups) were designed for dental casting Ti alloys. Prior to casting prccedure, mother alloys were prepared by using vacuum arc melting with skull melting method. Argon-arc melting and argon/vacuum-pressure casting machine were used for making the specimens. The hardness, microstructure, and corrosion resistance were evaluated by using Vickers microhardness measurement, FE-SEM, and anodic polarization test. The results were as follows: 1. The Vickers microhardness (Hv) of Ti and Ti-6Al-4V alloy showed the values of 210 and 352, and Ti-20Cr and Ti-20Zr alloys showed the values of 341 and 259, respectively. As the Cr content increased from 0 to 15 % in Ti-20Zr-XCr (X=0, 2, 5, 10, 15) alloys, the Hv increased from 259 to 298, 457, 344, and 351, respectively. The microstructure of Ti-20Zr-XCr specimens showed significant difference by the concentration changes of Cr content showing acicular crystalline structure for Ti-20Zr-2Cr and Ti-20Zr-5Cr, and equiaxed crystalline structure for Ti-20Zr-10Cr and Ti-20Zr-15Cr. 2. Open-circuit potentials (E_(∝)) were rapidly decreased followed by steadiness as time elapse in all experimental groups except in Ti-20Z group. Ti-2OZr group showed an increase in E_(∝), from the first. 3. In potentiodynamic anodic polarization tests, all experimental groups showed more noble corrosion potential (E_(corr) than that of Ti-6Al-4V, and more basic E_(corr) than that of Ti. Especially, Ti-20Zr-10Cr alloy showed most favorable corrosion resistance among Ti-Zr-Cr alloys. 4. For Ti-20Zr-10Cr, Ti-20Zr-15Cr, and Ti-20Cr groups, which have more than 10% Cr content, the secondary corrosion potential shifted to noble direction in larger amounts than in the cases of control and other experimental groups. From these results, it was concluded that newly formulated Ti-20Zr-10Cr experimental alloy have adequate hardness and high corrosion resistance, and this alloy is promising candidate for a successful dental casting alloy.

Influence of Oxygen Consumption on Pregnancy Rates of Hanwoo Calves following Embryo Transfer

Kim, Hyun,Bok, Nan-Hee,Kim, Sung-Woo,Do, Yoon-Jung,Seong, Hwan-Hoo,Kim, Jae-Hwan,Kim, Dong Hun,Kim, Min-Kyu,Ko, Yeoung-Gyu 한국수정란이식학회 2014 한국동물생명공학회지 Vol.29 No.3

Recently, several approaches have been used to measure the oxygen consumption rates of individual embryos, but relationship between oxygen consumption and pregnancy rates of Hanwoo following embryo transfer has not yet been reported. In this study, we investigated the correlation between oxygen consumption rate and pregnancy rates of Hanwoo embryo using a SECM. In addition to, the expression of apoptosis-related genes was determined using real-time PCR by extracting RNA according to the oxygen consumption of in vivo embryo. First, we found that the oxygen consumption significantly increased in blastocyst-stage embryos (blastocyst) compared to early blastocyst stage embryos, indicating that oxygen consumption reflects the embryo quality (Grade I). The oxygen consumption or GI blastocysts were significantly higher than those of GII blastocysts (10.2x1014/mol s-1 versus 6.4x1014/mol s-1, p<0.05). Pregnant rate in recipient cow was 0, 60 and 80% in the transplantation of embryo with the oxygen consumption of below 10.0, 10.0~12.0 and over 12.0x1014/mol s-1, respectively. Apoptosis regulatory genes, Hsp-70.1 were significantly increased in over-10.0 group than below 10.0 group but in Caspase-3, Bax and P53 gene, there was no significant difference. In conclusion, These results suggest that measurement of oxygen consumption maybe help increase the pregnant rate of Hanwoo embryos.

Characterizations of the bovine subtype Interferon-tau Genes : Sequences of Genes and Biological Activity of Transcription Factors in JEG3 Cell

Kim, Min-Su,Min, Kwan-Sik,Seong, Hwan-Hoo,Kim, Chan-Lan,Kim, Dongkyo,Imakawa, Kazuhiko,Kim, Sung Woo The Korean Society of Embryo Transfer 2016 한국동물생명공학회지 Vol.31 No.4

Multiple interferon tau (IFNT) genes exist in bovine. An antiluteolytic substance secreted by the bovine conceptus and primarily responsible for maternal recognition of pregnancy is bovine trophoblast protein 1 (bIFNT1), a new type I interferon tau (IFNT) genes. The objectives of this research were to investigate whether multiple, distinct gene encode bIFNT1 and other type I bIFNT gene in the bovine genome and to examine expression of bIFNT1 and other bIFNTc1 mRNAs during conceptus development. These transcrips could be regulated through caudal-related homeobox-2 (CDX2) and ETS2 and/or AP1 (JUN) expression, a transcription factor implicated in the control of cell differentiation in the trophectoderm. The presence of mRNAs encoded by bIFNT1 and type I bIFNTc1 genes were examined quantitatively via reverse transcription-polymerase chain reaction (RT-PCR) analysis of total cellular RNA (tcRNA) extracted from on day 17, 20 and 22 bovine conceptuses. The expression level of bIFNT1 was higher on day 17 transcripts were gradually weakly detectable on day 20 and 22. However, the other bIFNTc1 gene examined transcripts was highly expressed on day 20 and transcripts were weakly detectable on day 17 and 22 bovine conceptuses. Furthermore, human choriocarcinoma JEG3 was co-transfected with an -1kb-bIFNT1/c1-Luc constructs and several transcription factor expression plasmids. Compared to each -1kb-bIFNT1/c1-Luc increased when this constructs were co-transfected with, ETS2, AP1(JUN), CREBBP and/or CDX2. Also, bIFNTc1 gene was had very effect on activity by alone ETS2, and AP1 (JUN) expression factors in choriocarcinoma JEG3 cell. However, bIFNT1 gene expression of the upstream region was not identified. We demonstrated that the activities of bIFN genes are regulated by differential, tissue-specific and developmental competence during pregnancy.

Preclinical development of a humanized neutralizing antibody targeting HGF

Kim, Hyori,Hong, Sung Hee,Kim, Jung Yong,Kim, In-Chull,Park, Young-Whan,Lee, Song-Jae,Song, Seong-Won,Kim, Jung Ju,Park, Gunwoo,Kim, Tae Min,Kim, Yun-Hee,Park, Jong Bae,Chung, Junho,Kim, In-Hoo Nature Publishing Group 2017 Experimental and molecular medicine Vol.49 No.3

<P>Hepatocyte growth factor (HGF) and its receptor, cMET, play critical roles in cell proliferation, angiogenesis and invasion in a wide variety of cancers. We therefore examined the anti-tumor activity of the humanized monoclonal anti-HGF antibody, YYB-101, in nude mice bearing human glioblastoma xenografts as a single agent or in combination with temozolomide. HGF neutralization, The extracellular signal-related kinases 1 and 2 (ERK1/2) phosphorylation, and HGF-induced scattering were assessed in HGF-expressing cell lines treated with YYB-101. To support clinical development, we also evaluated the preclinical pharmacokinetics and toxicokinetics in cynomolgus monkeys, and human and cynomolgus monkey tissue was stained with YYB-101 to test tissue cross-reactivity. We found that YYB-101 inhibited cMET activation <I>in vitro</I> and suppressed tumor growth in the orthotopic mouse model of human glioblastoma. Combination treatment with YYB-101 and temozolomide decreased tumor growth and increased overall survival compared with the effects of either agent alone. Five cancer-related genes (TMEM119, FST, RSPO3, ROS1 and NBL1) were overexpressed in YYB-101-treated mice that showed tumor regrowth. In the tissue cross-reactivity assay, critical cross-reactivity was not observed. The terminal elimination half-life was 21.7 days. Taken together, the <I>in vitro</I> and <I>in vivo</I> data demonstrated the anti-tumor efficacy of YYB-101, which appeared to be mediated by blocking the HGF/cMET interaction. The preclinical pharmacokinetics, toxicokinetics and tissue cross-reactivity data support the clinical development of YYB-101 for advanced cancer.</P>

Characterizations of the Bovine Subtype Interferon-tau Genes*: Sequences of Genes and Biological Activity of Transcription Factors in JEG3 Cell

Min-Su Kim,Kwan-Sik Min,Hwan-Hoo Seong,Chan-Lan Kim,Dongkyo Kim,Kazuhiko Imakawa,Sung Woo Kim 한국수정란이식학회 2016 한국동물생명공학회지 Vol.31 No.4

Multiple interferon tau (IFNT) genes exist in bovine. An antiluteolytic substance secreted by the bovine conceptus and primarily responsible for maternal recognition of pregnancy is bovine trophoblast protein 1 (bIFNT1), a new type I interferon tau (IFNT) genes. The objectives of this research were to investigate whether multiple, distinct gene encode bIFNT1 and other type I bIFNT gene in the bovine genome and to examine expression of bIFNT1 and other bIFNTc1 mRNAs during conceptus development. These transcrips could be regulated through caudalrelatedhomeobox-2 (CDX2) and ETS2 and/or AP1 (JUN) expression, a transcription factor implicated in the control of cell differentiation in the trophectoderm. The presence of mRNAs encoded by bIFNT1 and type I bIFNTc1 genes were examined quantitatively via reverse transcription-polymerase chain reaction (RT-PCR) analysis of total cellular RNA (tcRNA) extracted from on day 17, 20 and 22 bovine conceptuses. The expression level of bIFNT1 was higher on day 17 transcripts were gradually weakly detectable on day 20 and 22. However, the other bIFNTc1 gene examined transcripts was highly expressed on day 20 and transcripts were weakly detectable on day 17 and 22 bovine conceptuses. Furthermore, human choriocarcinoma JEG3 was co-transfected with an -1kb-bIFNT1/c1-Luc constructs and several transcription factor expression plasmids. Compared to each -1kb-bIFNT1/c1-Luc increased when this constructs were co-transfected with, ETS2, AP1(JUN), CREBBP and/or CDX2. Also, bIFNTc1 gene was had very effect on activity by alone ETS2, and AP1 (JUN) expression factors in choriocarcinoma JEG3 cell. However, bIFNT1 gene expression of the upstream region was not identified. We demonstrated that the activities of bIFN genes are regulated by differential, tissue-specific and developmental competence during pregnancy.