Triton X-100이 Trichoderma koningii의 성장 및 형태에 미치는 영향

박희문,민경렴,맹필재,하영칠 충남대학교부설 생명공학연구소 1992 생물공학연구지 Vol.2 No.-

Triton Ⅹ-100이 Trichoderma koningii의 성장 및 형태에 미치는 영향을 조사하기 위하여, Triton Ⅹ-100 첨가 여부에 따른 각 종 성장계수의 변화를 조사하여 보았다. 액체 배양시 Triton Ⅹ-100의 첨가에도 불구하고, specific growth rate과 doubling time은 변화가 없었으나, Triton Ⅹ-100을 첨가한 경우, 군체의 방사상 성장률이 감소하였다. 이러한 현상은 Triton Ⅹ-100에 의하여 균사의 분지 형성이 촉진되고, 격막형성이 촉진되어 격막 간의 간격이 짧아져 나타나는 것으로 확인되었다. We investigated the effect of Triton Ⅹ-100 on the growth and morphology of Trichoderma koningii by comparing various parameters representing the growth of mold in the presence or absence of Triton Ⅹ-100. The specific growth rate and doubling time of T. koningii were not affected by the addition of 0.05% Triton Ⅹ-100 into solid medium also resulted in decrease in the colony radial growth rate and this response was correlated with the formation of mycelia which showed increase in branching and septation in the presence of Triton Ⅹ-100.

W-3계 고도 불포화지방산의 혼합비율이 다른 유지가 지질대사에 미치는 영향

박필숙,김성희,정승용,김경숙,문순열 慶尙大學校 1988 論文集 Vol.27 No.1

혈청 콜레스테롤 및 중성지방의 농도와 밀접항 관계가 있으며 혈청 lipprotein의 대사에 영향을 미치는 w-3계의 지방산의 적정한 섭취 비율을 구명하기 위한 일환으로써 들깨기름과 라아드의 혼합비율을 달리하여 흰쥐에게 급이한 결과 다음과 같은 결과를 얻었다. 1. 혈청 총-콜레스테롤 농도는 들깨기름 7.5%+라아드2.5%를 급이한 2군에서 유의성있게 낮은 반면 10%라아드 급이군은 현저히 높았다. 2. 혈청 HDL-콜레스테롤은 3군과 4둔이 1군, 2군에 비해 낮았으며, 혈청 총-콜레스테롤에 대한 HDL-콜레스테롤 비율은 2,3,4군이 5군에 비해 높았다. 3. 혈청 중 인지질과 중성지방 농도는 5군에 비해 2,3군에서 현저하게 낮았다. 4. 간장 중 총-콜래스테롤 농도는 4군이 1,2,3군에 비해 비교적 낮았다. 5. 간장 중 인지질 농도는 각 군간에 유의적인 차이는 없었으나 중성지방의 농도는 5군보다 1,2군에서 유의성 있게 낮았다. 6. 시험유지의 지방산조성은 1군에서 lionenic acid가 dir 58%이었으며, 5군에서는 w-3계 PUFA가 0.1%, oleic acid가 47.5%이었다. 7. 혈청 lipoprotein pattern은 2군에서 HDL함량이 가장 많은 반면 LDL함량은 가장 적은 경향이었다. In order to investigate the suitable composition of the w-3 series polyunsaturated fatty acid on lipoprotein metabolism related with cholesterol and triglyceride concentrations in serum, it was examined the effects of w-3 PUFA contents of mixed fat on oil lipid metabolism in serum and liver of rats. The results were summerized as follow ; 1. The concentration of T-cholesterol in serum of rats was lowest in the group 2 (7.5% perilla oil + 2.5% lard). whereas it was highest in group 5 (10% lard). 2. The concentration of HDL-cholesterol in serum was lower in group 3, 4 than in group 1, 2 and the ratio of HDL-cholesterol to T-cholesterol were higher in group 2, 3, 4 than in group 5. 3. The concentrations of phospholipid and trigyceride in serum were significantly lower in group 2, 3 than in group 5. 4. The concentration of T-cholesterol in the liver was relatively lower in group 4 than in group 1, 2, 3. 5. Phospholipid values in the liver were not significantly different between groups, but the concentrations of triglyceride were remarkably lower in group 1, 2 than those of the group 5. 6. Linolenic acid in group 1 (10% perilla oil), in the fatty acid composition of test lipids used in the experiment. was as much as about 58%, but w-3 PUFA in group 5 was only 0.1% and oleic acid was as much as about 58%, but w-3 PUFA in group 5 was only 0.1% and oleic acid was as much as 47.5%. 7. HDL contents, in lipoprotein composition of serum were the most in group 2 (7.5% perilla oil+2.5% lard), while LDL contents were the least.

중학교 가정 교과 수행평가 방법 및 도구 개발

박선영,조필교,이양심,윤인경,임양순,최창숙,장명희 한국 가정과 교육 학회 1998 한국가정과교육학회지 Vol.10 No.2

The purpose of the study is to develop a Performance Assessment Method & Tools for Home Economics at middle school. Through the content analyses of middle school Home Economics curriculum and 8 textbooks of lst, 2nd, and 3rd grade respectively, teching elements for each content area were drawn and each specific learning objectives were idntified accordingly. In addition, assessment element for student's performance in each teaching element were specified for analysis. Analytic framework for the development of Performance Assessment Method and Tools were proposed by each teaching element. Beased on the analytic framework proposed, a model method and tools were developed by 10 different test measures of description written test, statement written test, oral test, pro and con discussion, performance test, experiment, interview, observation, self-test report, and portfolio. The model proposed in the study has been modified and improved through the feasibility studies of experts' examination and field research application. The model assessment method and tools for the study has been compleeted finally by proposing 45 performance assessment tools for the content areas of food, clothing, housing, resource management and consumerism, human development and family relationship.

단기간의 체중감량이 유도선수들의 운동수행능력에 미치는 영향

박종필,장경태 韓國體育大學校附屬 體育科學硏究所 1997 韓國體育大學校附屬 體育科學硏究所論文集 Vol.16 No.1

To investigate the effects of short-term weight reduction and subsequent rehydration on performance of the judo players, eight college-age judo players performed cybex and 3 wingate tests, seperated by 45min or 1 hr, before and after weight reduction(5%). Muscle strength, anaerobic power, anaerobic capacity, blood lactate, and blood pH level were measured. The results were as follows: 1. There were no differences in muscle strength and total work capacity, measured by cybex, between pre and post weight reduction. 2. There were no differences in peak power and anaerobic capacity, measured by wingate test, between pre and post weight reduction. It was concluded that 5% weight reduction followed by 4 hours of food consumption did not decreased the physical capacity measured by multiple wingate tests.

CTX-M형 ESBL 생성 비장티푸스성 살모넬라의 특성

박순호,서일혜,안정열,박필환,김경희,송영희,김정은 대한감염학회 2010 감염과 화학요법 Vol.42 No.1

Background: Extended-spectrum ß-lactamase (ESBL)-producing Salmonella have been increasingly reported worldwide. ESBL-producing Salmonella is of particular concern since children cannot be treated with quinolones. This study was conducted to determine the phenotypic and genetic characteristics of ESBL-producing Salmonella in a tertiary hospital. Materials and Methods: Four clinical ESBL-producing isolates of non-typhoidal Salmonella were collected during 2001 to 2009. Antimicrobial susceptibility was determined by disk diffusion test and VITEK-II system. ESBL production was tested by ESBL phenotypic confirmatory test. TEM, SHV, CTX-M1, CTX-M2, CTX-M8, and CTX-M9 type ESBL genes were detected by PCR amplification, and PCR products were subjected to direct sequencing Results: Phenotypic confirmatory test showed that 4 of the 300 non-typhoidal Salmonella isolates were ESBL-producing: 3 S. Enteritidis and 1 S. Typhimurium. All 4 isolates were recovered during the past 1 year period. All 3 S. Enteritidis harbored CTX-M-15, while the S. Typhimurium harbored CTX-M-14. All CTX-M-15-producing S. Enteritidis isolates showed resistance both to cefotaxime and ceftazidime, while the CTX-M-14-producing S. Enteritidis were resistant only to cefotaxime. Conclusions: ESBL-producing nontyphoidal Salmonella has emerged recently and the type of ESBL has switched from TEM and SHV to CTX-M.

호흡 부전을 동반한 급성 자율-감각신경병증 1예

박경필,안규환,김은주,김대성,정대수,박규현,김매자 대한신경과학회 1999 대한신경과학회지 Vol.17 No.5

혼합 유전자형 검출(HLA-DQα, D1S80 and Vwa Loci)에 관한 연구

강필원,박기원,이경룡,최상규 內務部 國立科學搜査硏究所 1996 國立 科學 搜査 硏究所 年報 Vol.28 No.-

터널과 도로 및 교량에 대한 VE/LCC 분석에 관한 연구

안종필,박경호,임재춘 朝鮮大學校 建設技術硏究所 2006 建設技術硏究 Vol.26 No.1

It is actual state in our country that the number of tunnels on the road increases consistently but there is no study or computer program on life cycle cost Ihereinafter "LCC") of tunnel. This research is for the purpose of calculating the LCC for tunnel on the road and applying it to the systematic maintenance & management and analysis of economical efficiency. Further the developed computer program calculating the LCC for tunnel on the road increases the efficiency in tunnel design and in decision of investment considering the maintenance & management and the economical efficiency and it will be a useful tool with relation to VE/LCC technique. VE analysis is constituted of performance represented by P. life cycle cost represented by C and value represented by V. P is calculated under considering weight and degree of estimation which explain the importance of each facilities to the estimation item. C is determined from getting sum of the present prices of initial construction cost, maintenance & management cost during service life, break-up & disposal cost. V is obtained from dividing the P by C, that is V=P/C. The estimation list of the maintenance & management on tunnel has been set up as items whose maintenance & management cost was appeared to be relatively larger than the others and there were decision of the variables of effect such as frequency of maintenance & repair, unit cost, repairing rate, etc.. And also, all the cost were converted into present prices considering the economic variables for example discount rate, etc. Excel base LCC analysis system of tunnel on road was developed and tested on a sample tunnel.

Erythromycin 내성 포도알균의 유도형 Macrolide-Lincosamide-Streptogramin B (MLS_(B)) 내성 표현형 빈도

김경희,박순호,박필환,안정열,서일혜 대한감염학회 2010 감염과 화학요법 Vol.42 No.3

Background: Inducible MLS_(B) (macrolide-lincosamide-streptogramin B) resistance in staphylococci is not detected by standard susceptibility test methods. Failure to identify inducible MLS_(B) resistance may lead to clinical failure during clindamycin therapy. We determined the prevalence of inducible MLS_(B) resistance in erythromycin-resistant staphylococcal isolates. Materials and Methods: We evaluated all 2,792 non-duplicate staphylococcal strains: 1,402 Staphylococcus aureus and 1,390 coagulase-negative staphylococci (CoNS) isolated from May 2008-June 2009 at one-unoversity hospital. Testing for inducible MLS_(B) was accomplished by the disk approximation test (D-test) in accordance with the recommendations of the Clinical and Laboratory Standards Institute (CLSI). Results: Of the 2,792 staphylococcal isolates, 892 S. aureus isolates and 740 CoNS isolates were resistant to erythromycin. Among the 892 erythromycin-resistant S. aureus isolates, the overall prevalence of inducible MLS_(B) was 21.3% (16.2% of MRSA and 76.3% of methicillin-susceptible S. aureus). Among the 740 erythromycinresistant CoNS isolates, the overall prevalence of inducible MLS_(B) was 16.5% (16.0% of methicillin-resistant CoNS and 18.7% of methicillin-susceptible CoNS). The D-test was positive in 88.8% of S. aureus and 28.4% of CoNS isolates, which were erythromycin-resistant and clindamycin-susceptible. Conclusions: There are some variations in the prevalence of inducible MLS_(B) resistance in clinical staphylococcal isolates. It is important that clinical laboratories report inducible MLS_(B) resistance for erythromycin-resistant and clindamycinsusceptible staphylococcal isolates.

함수량이 다른 오염지반의 지지력 결정

안종필,박경호,백현 朝鮮大學校 建設技術硏究所 1999 建設技術硏究 Vol.19 No.1

This study, investigates the existing theoretical backgrounds in order to examine the behavior of lateral flow owing to the plasticity of soils when unsymmetrical surcharge is worked on polluted soils by the increase of water content, compares and analyzes the results measured through model tests. Unsymmetrical surcharge is increased at regular intervals to soil tank made up the polluted soils and then the amounts of settlement, lateral displacement and upheaval are observed. Critical surcharge was decided q_(cr)=3.42 C_(u) similar to those had proposed Terzaghi and JHI, and the value of ultimate capacity was decided q_(ult)=7.71 C_(u) similar to that of Tschebotarioff and JHI