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Vero E6 細胞에서 Semliki Forest Virus의 증식성에 관한 연구
이연태,강필원 대한바이러스학회 1988 Journal of Bacteriology and Virology Vol.18 No.1
In this study, Vero E6 cell, derived frorn African green monkey kidney, was selected as infectious host of Semliki forest virus and it was certified by cytopathic effect. Monolayer cells were infected with each serial diluted virus and maximum virus growth rate was obtained at 16 hours by freezing-solving methods. Ultra thin section samples were prepared to trace the infection route and the growth of virus. The majority of virus was trapped into coated pit region, internalized by endocytosis in coated vesicles and sequestered into intracellular vacuoles and lysosomes. Direct penetration of virus through the plasma membrane was never observed. After formation of coated vesicles, captured virus psrticles in lysosome were immediately released to cytoplasm and new virus particles were replicated there. Many virus particles were found in host cell organells, especially in vacuoles. Newly formed nucleocapsides acquired spike protein depending on host cell membrane by budding process, consequently new virus synthesis were completed. However, no penetration through nuclear membrane was observed. In the chromosome assay of viral infected cells, no significant chromosome aberration was found.