성인형 당뇨병 환자에서 피부생검을 이용한 표피하 모세혈관 기저막 두께의 변화에 관한 연구

김원학,오금탁,김만우,장경식,채종구,조건국 朝鮮大學校 附設 醫學硏究所 1987 The Medical Journal of Chosun University Vol.12 No.1

Thickening and proliferation of capillary basement membrane is a generalized phenomenon in diabetes mellitus and has been described in many organs including heart, kidney, pancreas, retina etc. While such changes are specific, it is difficult to obtain specimens from those organs. We took the specimens from the medial surface of the thing of 33 diabetics and 4 healthy persons as controls by means of punch biopsy. The measurement carried out by normogram and electron microscopic technique. HbA_(1c) values were also measured simultaneously. 1. The HbA_(1c) values are higher in diabetics than in control group (p<0.01). 2. The subepidermal capillary basement membrane thickness of the diabetics : 30% of the 5 th decade population, 53.9% of the 6 rh decade population and 83.3% of the 7th decade population showed more rhan 3,000 A. Whereas all the controls showed below 3,000 A. 3. The subepidermal capillary basement membrane thickness did nor increase significantly with the duration of the disease. 4. In cases of high subepidermal capillary basement membrane thickness, HbA_(1c) showed significant increase. (p<0.01).

Midazolam 이 안압에 미치는 영향

김완식,박동호,황영희,김흥대,김인규,서병태,김미연,정연탁 대한마취과학회 1982 Korean Journal of Anesthesiology Vol.15 No.1

Intraocular tension may be acutely changed by many drugs and by various physiologic events. An acute rise in intracoular tension may be catastrophic if it occurs when the globe is open and leads to expulsion of contents. Most general anesthetics cause a decrease in intraocular pressure, although a few causes increased intraocular pressure. Midazolam is a 1,4-benzodiazepine derivtive synthesized by Walser and Freyer in 1975. Eariler studies with midazolam have demonstrated its efficacy for induction of anesthesia and premedication. It is also desirable to know if all anesthetic agents which produce general anesthesia and which are pharmacologically different affect intraocular pressure in a similar manner. Therefore investigation of the influence of midazolam on intraocular pressure in 25 patients was undertaken at the Dept. of anesthesiology, Hanyang University. All patients had no known eye abnormalities. The patients were not premedicated. In all partients the intraocular pressure was measured before induction of anesthesia, after instilling a 0.5% tetracaine into the conjunctival sac. A second reading was taken after induction of midazolam (0.2 mg/kg of body weight) and a third after injection of succinylcholine (1 mg/kg of body weight) and a fourth after endotracheal intubation. A Schiotz tonometer with a 5.5 gm and a 7.5 gm weight was used. In addition to the tonometric determinations, the bood pressure, pulse rate and respiratory rate were recorded before and after induction of midazolam. An attempt was tried to keep the intraocular pressure changed as many and to minimize the other factors affecting intraocular pressure. To achieve this, supine position and constant gea flow was maintained. Special care was taken to avoid pressure on the patients eyes and to maintain a fully patent airway to prevent respiratory disturbances leading to straining and increased venous pressure. Endotracheal intubation was performed with the aid of succinylcholine to avoid cough or laryngospasm. The results of the observation with the above mentioned method were tested by student t-test statistically. Each patient acted as his own control. There was a fall in intraocular pressure in 17 patients among 25 parients (average 1.8 mmHg), but no significant change followed by the use of midazoam. The blood pressure variations were between 10 and 40 mmHg, during the course of anesthesia and could not be related to intraocular pressure changes. Intraocular pressure chagnes had no relation to pulse and respiratory rate variations. This finding indicated that benzodiazepine as a class of drugs have well described muscle relaxant properties that are primarily central (supraspinal) rather than peripheral (myoneural) in action. There was a rise in intraocular pressure in 19 patients among 25 patients, followed by the use of succinylcholine and 23 patients among 25 patients, after endotracheal intubation. According to Feldman and Crawley, diaxepam potentiated the myoneural blocking effects of gallamine and antagonizes the effects of succinylcholine. Nevertheless Dretchen demonstrated that the clinical doses of diaxepam did not potentiated the muscle relaants. Our finding showing no apparent succinylcholine interaction with midazolam are consistent with the finding of Dretche.

Peeling Back the Evolutionary Layers of Molecular Mechanisms Responsive to Exercise-Stress in the Skeletal Muscle of the Racing Horse

Kim, Hyeongmin,Lee, Taeheon,Park, WonCheoul,Lee, Jin Woo,Kim, Jaemin,Lee, Bo-Young,Ahn, Hyeonju,Moon, Sunjin,Cho, Seoae,Do, Kyoung-Tag,Kim, Heui-Soo,Lee, Hak-Kyo,Lee, Chang-Kyu,Kong, Hong-Sik,Yang, Yo Oxford University Press 2013 DNA research Vol.20 No.3

<P>The modern horse (<I>Equus caballus</I>) is the product of over 50 million yrs of evolution. The athletic abilities of the horse have been enhanced during the past 6000 yrs under domestication. Therefore, the horse serves as a valuable model to understand the physiology and molecular mechanisms of adaptive responses to exercise. The structure and function of skeletal muscle show remarkable plasticity to the physical and metabolic challenges following exercise. Here, we reveal an evolutionary layer of responsiveness to exercise-stress in the skeletal muscle of the racing horse. We analysed differentially expressed genes and their co-expression networks in a large-scale RNA-sequence dataset comparing expression before and after exercise. By estimating genome-wide <I>d</I><SUB>N</SUB>/<I>d</I><SUB>S</SUB> ratios using six mammalian genomes, and F<SUB>ST</SUB> and iHS using re-sequencing data derived from 20 horses, we were able to peel back the evolutionary layers of adaptations to exercise-stress in the horse. We found that the oldest and thickest layer (<I>d</I><SUB>N</SUB>/<I>d</I><SUB>S</SUB>) consists of system-wide tissue and organ adaptations. We further find that, during the period of horse domestication, the older layer (F<SUB>ST</SUB>) is mainly responsible for adaptations to inflammation and energy metabolism, and the most recent layer (iHS) for neurological system process, cell adhesion, and proteolysis.</P>

적절한 임프란트 식립을 위한 이공과 이관의 해부학적 고려사항

Kyu-Tag Kim,Jae-Ho Yang,Sang-Bong Kim,Jong-Tae Park,Koung-Youn Lee,Hyun Sook Bae,JOO-CHEOL PARK,Heung-Joong Kim 대한구강해부학회 2007 대한구강해부학회지 Vol.31 No.1

The menta1 foramen is located between the first and second premolar regions. The direction of mental cana1 is cornmonly described as being outward, backward, upward from its origin at the mandibular canal‘ The mental nerve is a terrninal branch of the inferior alveolar nerve that passes through the menta1 foramen, supplying sensory innervation to the lower lip, bucca1 vestibule, and gingiva of the mandibular premolar regions. The purpose of this study was to investigate the position of the mentaJ foramen and the length and the direction of the mentaJ canal in Korean. 1n this study, 41 aduJt dry mandibles were investigated independentJy by four observers. The position of the mental foramen and the distance from the mandibular inferior border were measured using conventionaJ nder. The Jength of the menta1 canal was measured directJy using periodontaJ probe. The horizontal and vertical angJes of the mental cana1 were measured by protractor. The student’s T - test was carried out to assess right and Jeft side difference. The results were as foJJows: 1. The majority of the mental foramen were Jocated below the second premolar' (66.1%), and then between the first and second premoJars(27.4%)‘ 2. The distance between the mental foramen and the inferior border of mandible was 14 .4l:!:1. 47mm(mean 土SD) in right side and 14.36:!:l.42mm in left side(P>O.05) 3. The Jength of the mental canal was 7.8l:!:1.12mm in right side and 7.95:t1.ODmm in Jeft side(P>O.05). 4. The horizonta1 angle of the mentaJ canal was 24.26土5.470 in right side and 24.8l:!:4.7870 in Jeft side(P>O‘ 05) 5‘ The vertical angle of the mental canal was 27.34土4.550 in right side and 28.79:t4.11 0 in left side(P>O.051. 6. The length of anterior Joop of the mental canal was 7.1l:!:1.l5mm in right side and 7.07:tO.99mm in Jeft side(P>O.05) These results suggest that dentist have to consider the mental foramen and menta1 canal when attempting to achieve regiona1 anesthesia, periodontal surgery, and endosseous implants

NDT기법을 이용한 피복전선 검출장치 개발

김탁용(Tag-Yong Kim),오용철(Yong-Cheul Oh),이동규(Dong-Kyu Lee),정한석(Han-Seok Cheong),박건호(Geon-Ho Park),김기준(Ki-Joon kim),김충혁(Chung-Hyeok Kim),이덕진(Deok-Jin Lee),현득창(Deuk-Chang Hyun) 대한전기학회 2014 대한전기학회 학술대회 논문집 Vol.2014 No.7

동절기 실내 과습방지를 위한 자동환기시스템 제어장치 개발에 관한 연구

김영탁(Kim, Young-Tag),조우진(Cho, Woo-Jin),이규남(Rhee, Kyu-Nam),황민규(Hwang, Min-Kyu),이성재(Lee, Sueng-Jae),차지형(Cha, Ji-Hyoung) 한국태양에너지학회 2012 한국태양에너지학회 학술대회논문집 Vol.2012 No.11

The surface condensation in winter season can be prevented with two methods. The first method is increasing surface temperature of space envelopes with insulation and the second method is decreasing indoor vapor pressure with ventilation. But additional insulation after construction completion is not easy. So, ventilation is more suitable to solve condensation problems. Generally, there are two types of ventilation methods in residential buildings. One is natural ventilation by opening windows, but the efficiency defends on occupant’s action whether they open the windows or not. The other is mechanical ventilation by recovery ventilator(air-to-air heat exchanger). This can provide not only regular ventilation but also high air quality and less condensation problems, if it can be controlled automatically. Therefore, it is necessary to develop automatic control device of ventilation system for preventing condensation with decreasing indoor vapor pressure. To achieve it, this paper suggests CPA(Condensation Preventing Algorithm) for the auto-control device of ventilation system.

Investigation of Single Nucleotide Polymorphisms in Porcine Candidate Gene for Growth and Meat Quality Traits in the Berkshire Breed

Sang Wook Kim(김상욱),Ji Hye Jung(정지혜),Kyung Tag Do(도경탁),Kwan Suk Kim(김관석),Chang Hee Do(도창희),Jun kyu Park(박준규),Young Kuk Joo(주영국),Tae Suk Kim(김태숙),Bong Hwan Choi(최봉환),Tae Hun Kim(김태헌),Ki Duk Song(송기덕),By 한국생명과학회 2007 생명과학회지 Vol.17 No.12

4개의 후보 유전자를 분석해본 결과, 돼지의 주요 염색체 부위 및 유전자들이 주요 경제성 요인들과 관계가 있는 것으로 확인됐다. 양돈업계에서 DNA 기술을 이용한 염색체 정보를 활용하기 위해 본 연구에서는 4개의 후보 유전자에서 생성된 중합효소연쇄반응(PCR) 생성물을 비교 재 서열 함으로써 단일염기변이(SNP) 표지들을 개발했다. 또한 이들 4개의 SNP에 대해 PCR 제한효소 절편길이 다형 성(RFLP) 분석을 전개한 후, 이를 대한민국내 버크셔 종 돼지 개체군의 유전자형을 분석하는데 활용했다. 본 연구는 유용한 단일염기변이를 식별하고 돼지개체군 내 경제적으로 중요한 특성들과 SNP의 연관성을 확인하는 데 그 목적이 있다. This study was conducted to identify useful single nucleotide polymorphisms (SNPs) and determine their association with economically important traits in pig population. Four candidate gene analyses have identified important chromosomal regions and major genes associcated whit economic traits of the pig. For application of the chromosomal information to the pig industry using DNA technology, SNP markers were developed by comparative re-sequencing of polymerase chain reaction (PCR) products of 4 candidate genes (CSF2, IL4, MYOD, RIP140). PCR restriction fragment length polymorphism (PCR-RFLP) assays were developed for these 4 SNPs and used to genotype Berkshire pig populations in Korea.

증례 : 소화기 ; 식도와 위기능 장애가 동반된 만성 가성 장폐쇄증 1예

김부경 ( Bu Kyung Kim ),박무인 ( Moo In Park ),박선자 ( Seun Ja Park ),김규종 ( Kyu Jong Kim ),문원 ( Won Moon ),탁희상 ( Hee Sang Tag ),김성빈 ( Sung Bin Kim ) 대한내과학회 2011 대한내과학회지 Vol.81 No.2

반복적인 복통과 구토를 주소로 내원하여 임상증상과 병력, 고해상도 식도내압검사와 위배출 시간 검사를 통하여 식도와 위기능 장애가 동반된 소장의 만성 특발성 가성 장폐쇄증으로 진단되었고, 보존적 치료로 호전된 비교적 경한 증상의 증례이다. Chronic intestinal pseudo-obstruction (CIPO) is a rare digestive syndrome characterized by symptoms and signs of intestinal obstruction in the absence of mechanical obstruction. A 48-year-old female presented at our facility with severe abdominal pain and vomiting. Simple abdominal radiography revealed small bowel gas and ileus. Computed tomography also revealed a dilated small bowel, but there was no evidence of mechanical obstruction. Esophageal function was decreased based on high-resolution manometry and the gastric emptying time was prolonged on a gastric emptying scan. The patient recovered with conservative treatment. We report a case of chronic intestinal pseudo-obstruction with esophageal and gastric dysfunction. (Korean J Med 2011;81:223-228)

Copy Number Deletion Has Little Impact on Gene Expression Levels in Racehorses

Park, Kyung-Do,Kim, Hyeongmin,Hwang, Jae Yeon,Lee, Chang-Kyu,Do, Kyoung-Tag,Kim, Heui-Soo,Yang, Young-Mok,Kwon, Young-Jun,Kim, Jaemin,Kim, Hyeon Jeong,Song, Ki-Duk,Oh, Jae-Don,Kim, Heebal,Cho, Byung-W Asian Australasian Association of Animal Productio 2014 Animal Bioscience Vol.27 No.9

Copy number variations (CNVs), important genetic factors for study of human diseases, may have as large of an effect on phenotype as do single nucleotide polymorphisms. Indeed, it is widely accepted that CNVs are associated with differential disease susceptibility. However, the relationships between CNVs and gene expression have not been characterized in the horse. In this study, we investigated the effects of copy number deletion in the blood and muscle transcriptomes of Thoroughbred racing horses. We identified a total of 1,246 CNVs of deletion polymorphisms using DNA re-sequencing data from 18 Thoroughbred racing horses. To discover the tendencies between CNV status and gene expression levels, we extracted CNVs of four Thoroughbred racing horses of which RNA sequencing was available. We found that 252 pairs of CNVs and genes were associated in the four horse samples. We did not observe a clear and consistent relationship between the deletion status of CNVs and gene expression levels before and after exercise in blood and muscle. However, we found some pairs of CNVs and associated genes that indicated relationships with gene expression levels: a positive relationship with genes responsible for membrane structure or cytoskeleton and a negative relationship with genes involved in disease. This study will lead to conceptual advances in understanding the relationship between CNVs and global gene expression in the horse.

위턱뼈에서 미니임프란트 식립을 위한 물렁조직과 겉질뼈의 두께

박종태(Jong-Tae Park),정려령(Rye-Ryeng Jeong),김규탁(Kyu-Tag Kim),김상봉(Sang-Bong Kim),허경석(Kyung-Seok Hu),김희진(Hee-Jin Kim),임성훈(Sung-Hun Lim),김흥중(Heung-Joong Kim) 대한체질인류학회 2008 해부·생물인류학 (Anat Biol Anthropol) Vol.21 No.3

위턱뼈의 정중입천장봉합 부위와 어금니 사이는 교정용 미니임플란트를 식립하기에 적절한 장소이다. 이 연구는 물렁조직과 겉질뼈의 두께와 관련하여 미니임플란트를 가장 적절한 부위에 위치시키기 위한 장소에 대한 자료를 제공하고자 수행되었다. 연구용 사체 15구의 위턱뼈를, 정중입천장봉합 부위와 위턱 어금니 사이의 물렁조직과 겉질뼈의 두께를 계측하기 위해, 정중시상면과 볼쪽-입천장쪽면으로 절단하였다. 각 절단원 절편들을 스캐너로 스캔한 다음, 물렁조직과 겉질뼈의 두께를 측정하였다. 정중시상절편에서는 안쪽앞니 잇몸유두로부터 5㎜ 간격으로 6개의 지점의 두께를 측정하였고, 볼쪽-입천장쪽 절편에서는 이틀능선으로부터 치아뿌리끝쪽으로 1㎜ 간격으로 5개의 지정의 두께를 측정하였다. 정중입천장봉합 부위에서 물렁조직의 두께는 안쪽앞니 사이 잇몸유두로부터 15㎜ 지점에서 1.46㎜를 보였고, 그 뒤쪽에서 비교적 일정하다가 잇몸유로부터 35㎜ 지점 이후부터 급격히 증가하였다. 겉질뼈의 두께는 안쪽앞니 잇몸유두로부터 20㎜ 지점에서 2.13㎜로 가장 두꺼웠고, 그 뒤쪽 부위에서 일정한 두께를 보이다가 잇몸유두로부터 30㎜ 지점 이후부터 감소하였다. 입천장쪽의 물렁조직의 두께는 세 군 모두에서 이틀능선에서 1㎜ 지점에서 가장 얇았으며, 치아뿌리끝쪽으로 갈수록 점차 증가하였다. 입천장쪽 겉질뼈의 두께는 세 군 모두에서 이틀능선에서 1㎜ 지점에서 가장 두꺼웠으며, 치아뿌리끝쪽으로 갈수록 감소하였으나 그 차이는 미약하였다. 볼쪽 물렁조직의 두께는 세 군 모두에서 이틀능선에서 1㎜ 부위에서 가장 두꺼웠으며, 치아뿌리끝쪽으로 갈수록 점차 감소하였다. 볼쪽 겉질뼈의 두께는 세 군 모두에서 이틀능선에서 1㎜ 지점에서 가장 앓았으며, 치아뿌리끝쪽으로 갈수록 약간 증가하였다. 입천장쪽과 볼쪽을 비교하였을 때, 물렁조직의 두께는 입천장쪽이 더 두꺼웠고, 겉질뼈의 두께는 볼쪽이 입천장쪽보다 두꺼웠다. 위의 결과들은 위턱뼈 정중입천장봉합 부위와 어금니 사이에 미니임폴란트 식립 시 안전한 부위를 결정하는데 도움이 되는 물렁조직과 겉질뼈의 두께에 대한 해부학적 자료를 제공할 수 있을 것으로 생각된다. The midpalatal suture area and maxillary interdental area are suitable site for the placement of orthodontic mini-implant. The purpose of this study was to provide a guideline to indicate the best location for mini-implant placement as it relates to the thickness of soft tissue and cortical bone. Fifteen maxilla from 15 cadavers were cut in midsagittal plane and buccopalatal plane to measure the thickness of soft tissue and cortical bone of midpalatal and maxillary posterior interdental areas. Sectioned samples were scanned and the thickness was measured. The thickness of soft tissue and cortical bone were measured at 6 points from the interdental papilla with 5-mm intervals in the mid-sagittal section. And, the thickness of soft tissue and cortical bone were also measured at 5 points from the alveolar crest with I-mm intervals in the buccopalatal section. The mean and standard deviation of the measurement were calculated. Soft tissue thickness at the midpalatal suture area was 1.46 ㎜ at 15 ㎜ from the interdental papilla and remained uniformly thick posterior to this point, and steeply increased at 35 ㎜ area posteriorly. Cortical bone thickness were greatest (2.13 ㎜) at 20 ㎜ from the interdental papilla and remained uniformly thick posterior to this point, and decreased at 30 ㎜ area posteriorly. Palatal soft tissues thickness in all groups was thinnest at the 1 ㎜ from the alveolar crest and gradually increased from alveolar crest to apical portion. Cortical bone thickness in all groups was thickest at the 1 ㎜ from the alveolar crest and slightly decreased from alveolar crest to apical portion. Buccal soft tissue thickness in all groups was thickest at the 1 ㎜ from the alveolar crest and gradually decreased from alveolar crest to apical portion. Cortical bone thickness in all groups was thinnest at the 1 ㎜ from the alveolar crest and slightly increased from alveolar crest to apical portion. Soft tissue thicknesses were greater on the palatal side than on the buccal side. Cortical bone thicknesses of the buccal side were thicker than the palatal side. These results provide anatomical data of soft tissue and cortical bone thickness to assist in the determination of safe location for the mini-implant placement in the midpalatal and maxillary interdental areas.