모체의 혈중 단백 및 스테로이드 호르몬 측정을 이용한 절박유산의 예후 판정

김기원(KW Kim),함재홍(JH Ham),김대훈(DH Kim),김도강(DK Kim),이진우(JW Lee) 대한산부인과학회 1980 Obstetrics & Gynecology Science Vol.23 No.4

임신 제5-20주의 정상임신(대조군) 241예와 절박유산(실험군) 82예중 정상임신 진행군 38예 및 유산군 44예의 832 가검물로부터 24시간 뇨중 hCG, 혈중hPL, hPRL, AFP, estradiol-17β, estriol, progesterone, testosterone 및 cortisol을 측정하여 대조군 및 절박 유산 각군의 임신 주수에 따른 변동상황을 관차하고 측정치들의 분포와 절박유산의 예후를 비교 관찰한 결과 다음과 같 은 결론을 얻었다. 1. 대조군의 뇨중 hCG, 혈중 hPL, AFP, estradiol-17β, estriol, progesterone, testosterone 및 cortisol등 9가지 호르몬 또 는 혈청단백의 임신 각주에 따른 변동을 측정하였던 바 대다수의 다른 관찰자들과 일치 하였다. 2. 일반적으로 이들 9가지 호르몬 또는 혈청단백 측정의 모든 경우에서 절박유산군 중 정상임신 진행군의 임신 각주에 따른 측 정치는 대조군보다는 낮았지만 유의한 차는 없었다. 3. 일반적으로 이들 9가지의 호르몬 또는 혈청단백 측정의 모든 경우에서 절박유산군 중 유산군에서 임신 각주에 따른 측정치는 대조군보다는 현저하게 낮았고 이들중 대조군 및 정상임신 진행군 양자 모두에 비하여 유의한 차를 보인 것은 뇨중 hCG, 혈중 hPL, hPRL 및 estradiol-17β로서 이 4가지 호르몬 측정이 절박유산 예후판정의 가장 좋은 지표이었다. 4. 대조군에서 임신 각주의 평균-표준편차 이상의 측정치를 정상범위로 그 미만의 측정치를 정상범위 미만으로 간주하였을 때 뇨중 hCG, 혈중 hPL, hPRL 및 estradiool-17β의 절박유산 예후판정율은 각각 76.8, 80.9, 86.1 및 83.7%이었다. 5. 이들 4가지 호르몬 측정치중 임의의 2, 3 및 4가지를 동시에 고려하였을 때의 전반적인 정상임신 진행판정율은 각각 92.7, 97.7 및 100.0%이었다. 6. 이들 4가지 호르몬 측정치중 임의의 2, 3 및 4가지를 동시에 고려하였을 때의 전체적인 유산판정율은 각각 95.9, 99.1 및 100.0%이었다. 7. 이상의 결과로 보아 절박유산의 예후를 생화학적 검사로 측정 감시하기 위하여는 뇨ㅈ hCG, 혈중 hPL, hPRL 및 estradiol-17 β의 변동 중 적어도 3가지 이상을 동시에 관찰함으로써 거의 완전한 결과를 얻을 거승로 기대된다. For the purpose of evaluating the prognosis of the patients of threatened abortion the 24 hour-urinary titer if human chorionic gonadotropin, serum levels of placeful lactogen, prolactin, alpha-fetopretein, estradiol-17b, total estriol, progesterone, testosteronr and cortisol in 862 urine and serum samples from 241 cases of narmal pregnancy and 82 cases of threatemed abortion were measures weelky from 5th to 20th gestational weeks. Thw group of threatened abortion was again divided into 2 subgroups, that of 38 cases of favorable outcome in which the cases progress to narmal pregnancy up to at least 28th gestational weeks and that of 44 cases poor pregbosis in which the cases terminated in abortion within 28th gestational weeks. Thw changing patterns of all the data of 3 groups according to their gestational weeks were compared with each other. And considering certain data above the value of Mean minus I.S.D. of control group as normal and those below that value as subnormal all the data were compared with their own prognosis. Thw result were as follows. 1) In the subgroup of favorable outcome of threatened abortion, the levels of all 9 parameters of urinary hCG, serum hPL, hPRL, AFP, estradiol-17b, total estriol, progesterone, testosterone andcortisol were considerably lower than those of control group in geneal but were not significant statistically. 2) In the subgroup of abortion all those of 9 parameters were signifiantly lower than those of control group but those of only 4 parameters of urinary hCG, serum hPL, hPRL and estradiol-17b were significantly lower than those of both the control group and the subgroup of favourable putcome in general. 3) The accuracy rates to predic: the prognosis of threatened abortion with each of most rliable 4 hormonal parameters- urinary hCG, serum gPL, hPRL and estradiol-17b - were 76.8, 80.9, 86.1 and 83.7% respectively. 4) The overall accuracy rates to predict favorable outcome with any 2, 3 and all 4 normal rnages among most reliable above 4 hormaonal parameters were 92.7, 97.7 and 100.0% respectively. 5) The overall accuracy rates to predict abortion with any 2, 3, all 4 subnormal ranges among most reliable above 4 hormonal parameters were 93.9, 99.1 and 100.0% respectively. 6) Considering the results mentioned above, measurement of 4 parameters of urinary hCG, serum hPL, hPRL and estadiol-17b evaluating at least 3 ones simultaneously can be preferred in evaluating and predicting the prognosis of threatened abortion.

Brain abnormalities in Sjogren syndrome with recurrent CNS manifestations: association with neuromyelitis optica

Min, JH,Kim, HJ,Kim, BJ,Lee, KW,Sunwoo, IN,Kim, SM,Kim, BJ,Kim, SH,Park, MS,Waters, P,Vincent, A,Sung, JJ,Lee, KH SAGE Publications 2009 Multiple sclerosis journal: clinical and laborator Vol.15 No.9

<P><B>Background and objectives</B></P><P>Optic neuritis or longitudinally extensive myelitis in Sjogren syndrome (SS) suggests a neuromyelitis optica spectrum disorder (NMOSD). However, brain abnormalities of SS remain to be elucidated for the association with neuromyelitis optica (NMO).</P><P><B>Methods</B></P><P>Twelve primary SS patients (all women, 42?±?13.2?years) who had recurrent central nervous system (CNS) manifestations with brain involvement were retrospectively identified. Brain MRI, and neurologic and serologic findings were analyzed with the measurement of anti-aquaporin-4 antibody (AQP4-Ab).</P><P><B>Results</B></P><P>All patients showed brain lesions characteristic of NMO as follows: 1) the involved sites adjacent to the third and fourth ventricles and in the posterior limb of the internal capsule, 2) unique configurations, such as the longitudinal course from the internal capsule to the midbrain, large cerebral or cerebellar lesions over 3?cm, and cavity-like formations. AQP4-Ab was positive in six of eight patients tested, and all the seropositive patients showed lesions with increased diffusion, suggestive of vasogenic edema. Four patients met the revised criteria of NMO, and nine had features of NMOSDs. Of the remaining three patients showing only brain involvement, one had AQP4-Ab.</P><P><B>Conclusions</B></P><P>This study demonstrates that SS patients with recurrent CNS involvement have brain abnormalities characteristic of NMO and AQP4-Ab in Korea. The presence of AQP4-Ab in one SS patient with only brain involvement may suggest that the coexistence of NMO should be explored in SS patients with recurrent CNS manifestations, even without optic neuritis or myelitis.</P>

Dysregulation of the Wnt/β-catenin signaling pathway via Rnf146 upregulation in a VPA-induced mouse model of autism spectrum disorder

Park Gaeun,Jang Wooyoung Eric,Kim Seoyeon,Gonzales Edson Luck,Ji Jungeun,Choi Seunghwan,Kim Yujin,Park Ji Hwan,Mohammad Hazara Begum,Bang Geul,Kang Minkyung,Kim Soobin,Jeon Se Jin,Kim Jin Young,Kim Kw 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

Autism spectrum disorder (ASD) is a neurodevelopmental disorder associated with impaired social behavior and communication, repetitive behaviors, and restricted interests. In addition to genetic factors, environmental factors such as prenatal drug exposure contribute to the development of ASD. However, how those prenatal factors induce behavioral deficits in the adult stage is not clear. To elucidate ASD pathogenesis at the molecular level, we performed a high-resolution mass spectrometry-based quantitative proteomic analysis on the prefrontal cortex (PFC) of mice exposed to valproic acid (VPA) in utero, a widely used animal model of ASD. Differentially expressed proteins (DEPs) in VPA-exposed mice showed significant overlap with ASD risk genes, including differentially expressed genes from the postmortem cortex of ASD patients. Functional annotations of the DEPs revealed significant enrichment in the Wnt/β-catenin signaling pathway, which is dysregulated by the upregulation of Rnf146 in VPA-exposed mice. Consistently, overexpressing Rnf146 in the PFC impaired social behaviors and altered the Wnt signaling pathway in adult mice. Furthermore, Rnf146-overexpressing PFC neurons showed increased excitatory synaptic transmission, which may underlie impaired social behavior. These results demonstrate that Rnf146 is critical for social behavior and that dysregulation of Rnf146 underlies social deficits in VPA-exposed mice.

導入 機器의 效率的 活用을 위한 敎授-學習 敎材의 開發 : 8 個 學科 共同 硏究

李昌甲,孟琦錫,李載元,朴萬植,廷吉煥,金鍾悟,朴景采,李英浩,李東周,邕章祐,柳盛淵,趙澤東,姜理錫,李殷雄,李興浩,金健中,韓相玉,金良模,張錫明,朴俊灝,洪鳳植,金太均,金再韓,宋海永,盧載星 충남대학교 공업교육연구소 1982 論文集 Vol.5 No.1

In this study, through the combined effort and collaboration of 8 engineering education departments, teaching-learning processes and models were reviewed, and printed and bound student manuals developed on 120 different tasks and subjects. Audio-visual supportive material, already in use in laboratories, was added to form a complete teaching-learning package. In addition to promoting the maximum use of the newly acquired equipment, this study will also contribute to the development of laboratory practice, the in-service training of technical high schools teachers and help in the development of their own instructional program.

[Free Paper] Impact of patient's age on the development of new brain infarction after carotid endarterectomy and carotid artery stenting in Korean patients

KW Yoon,YJ Park,SS Yang,KM Park,DI Kim,YW Kim 대한외과학회 2011 대한외과학회 학술대회 초록집 Vol.2011 No.11

인슐린비의존형 당뇨병환자에서 Cilostazol(Pletaal)의 투여가 혈청 지질에 미치는 영향

김성연,이현철,이광우,강무일,송영득,허갑범,김영설,최영길,김태화,김현만 대한당뇨병학회 1995 Diabetes and Metabolism Journal Vol.19 No.4

우측 대장암 환자 문맥혈에서의 Cytokeratin 19 및 20의 발현

김홍,김봉완,김혜진,서광욱 대한대장항문학회 2003 Annals of Coloproctolgy Vol.19 No.5

Nd-YAG Laser를 이용한 가토의 미세 난관 문합술에서 문합부의 조직학적 소견

김장흡,이진우,김수평,김진홍,김은중,정기욱,권동진,고영미 대한산부인과학회 1997 Obstetrics & Gynecology Science Vol.40 No.10

To study tubal adhesion, tubal patency and histologic difference under light, electronic microcopy in a site of anastomosis, authors performed three anastomosis surgery in rabbit fallopian tube;1-layer and 2-layer anastomosis in splint-not-used group(group 1) and in splint-used group(group 2) and laser anastomosis in splint-used group(group 3). 10 rabbits were used in each group, therefore the total number of experimental rabbits in 3 groups were 30. The results were as follows; 1. The degree of gross adhesion in group Ⅱ was significantly high as compared with other groups but there was no difference between left and right tube in the degree of adh- esion in each group(Table 1). 2. The incidence of tubal patency in group Ⅱ was significantly high(89%) as compared with group Ⅰ(44%). Especially, the incidence of tubal patency in group Ⅲ was significantly high(100%) but there was no difference between left and right tube in incidence of tubal patency in each group. 3. Inflammatory cell infiltration appeared in each group on optical microscopic examin- ation. Especially, fibrosis and inflammatory cell infiltration appeared more in group Ⅱ but there was no statistically significant difference. And there was no significant difference between left and right tube in each group. 4. Transmission electron microscopic examination of group I, as compared with normal control group, showed increase of microvilli and secretory granules and decrease in size of epithelial cell. Epithelial cell was morphologically transformed but maintained the original structure comparatively. TEM examination of group Ⅱ, as compared with normal control group, showed decrease of microvilli and secretory granules and flattening of epithelial cell. It showed that epithelial cell vanished in it`s original structure. TEM examination of group Ⅲ, as compared with normal control group, showed decrease of microvilli and increase of secretory granules and morphological transformation of epithelial cell. Group Ⅲ showed the resemblence to normal control group. From the above results, in reversal tuboplasty and tubal factor infertility, it is consi- dered that simple splint-used anastomosis or splint-used anastomosis with Nd-YAG laser will improve the successful rate of tubal anastomosis.

토끼난관 미세문합수술에서 문합부의 성장인자 (TGF) 발현의 비교

김장흡,이진우,김수평,김진홍,김은중,정기욱,권동진,노승혜 대한산부인과학회 1997 Obstetrics & Gynecology Science Vol.40 No.11

Twenty seven rabbits were divided into 3 groups of 1, 2-1 and 2-2(n=9 in each group). Arbitarilly cut ends of mid-ampullar portion of each tubes were microscopically reanastomosed using vicryl as suture material without intraluminal splint in group 1, using vicryl as suture material with intraluminal splint in group 2-1 and coagulation by Nd-YAG Laser beam as ref-usion method with intraluminal splint in group 2-2. A week after operation, the tubal tissue segments of reanastomosis were embedded into paraffin to examine the expression rate and intensity of TGF-β1, 2 and 3 by immunohistochemical staining. The results were as follows. 1. The expression rate of TGF-β1, 2 and 3 revealed no stastically significant difference between group 1 and 2. 2. Among comparisons group 2-1 and 2-2, the TGF-β1 expression rate in group 2-2 showed lower value than that in group 2-1 with stastical significance(P$lt;0.05). The above fact that tuboplasty with Nd-YAG Laser using intraluminal splint resulted in lower expression rate of TGF β1 may suggest raising the succe ss rate of pregnancy.

Doppler 초음파를 이용한 월경주기별 자궁동맥과 난소동맥의 혈류속도파형의 연구

김장흡,이진우,김수평,정기욱,권동진,김진흥 대한산부인과학회 1995 Obstetrics & Gynecology Science Vol.38 No.7

1993년 12월부터 1994년 2월까지 가톨릭대학교 의과대학부속 성모자애 병원 산부인과 외래 를 내원한 규칙적인 월경주기를 가진 건강한 25명의 여성을 대상으로 질식도플러초음파를 이용하여 월경주기에 따른 자궁동맥과 난소동맥의 PI를 측정하여 다음과 같은 결과를 얻었다. 1. 월경주기에 따른 자궁동맥 PI의 변화 월경주기에 따른 자궁동맥 PI값은 전체 대상군의 경우 난포초기에 가장 높았으며 난포후기에 감소하는 양상을 보인 후 황체초기에 다시 증가한 후 감소하였다. 그러나 각 월경주기 간에 는 유의한 차이가 없었다. Dominant follicle또는 non-dominant follicle부위에서의 월경주기에 따른 자궁동맥 PI값의 변화는 전체대상군의 경우와 같은 양상을 보였으며 유의한 차이는 없 었다. 또한 각 월경주기에 있어서 dominant follicle과 non-dominant follicle간의 PI값은 유의한 차이가 없었다. 2. 월경주기에 따른 난소동맥 PI의 변화 월경주기에 따른 난소동맥 PI값은 전체 대상군의 경우 난포초기에 가장 높았으며 황체후기까지 감소 하는 양상을 보였다. 각 월경주기간의 PI 값은 유의한 차이를 보였다(p$lt;0.05). dominant follcle 또는 non-dominant follicle 부위에서의 월경주기에 따른 난소동맥 PI값의 변화는 전체 대상군의 경우와 같은 양상을 보였으며 각각 유의한 차이를 보였다(p$lt;0.01, p$lt;0.05). 또한 각 월경주기에 있어서 dominant follicle과 non-dominant follicle간의 PI값은 유의한 차이가 없었다. 이상의 결과로써 자궁동맥과 난소동맥의 PI값은 월경주기와 연관이 있으며, 특히 자궁동맥의 PI값은 월경에 의한 자궁내막의 탈락과 배란 후 수정란의 착상과 연관된 자궁의 혈류변화를 반영하는 것으로 생각되고 난소동맥의 PI값은 난포발달과 황체형성을 위한 난소의 혈류변화를 반영하는 것으로 생각된다. There is still a need for more information to clarify the changes that occur in uterine and ovarian blood flow during the menstrual cycles . Recently, the introduction of transvaginal color and pulsed wave doppler system has allowed the possibility of studying uterine and ovarian blood flow velocity. the purpose of this study was no examine blood flow velocity waveforms of the uterine and ovarian arteries during the normal menstrual cycle using transvaginal color and pulsed wave doppler ultrasonography. We studied 25 healthy women with a regular menstrual cycle and no history of previous ovarian surgery and medication. Blood folw impedance as reflected by the pulsatility index(PI) was determined for both arteries on 200 occasions. There was no significant difference in the PI values between the right and left arteries. In the uterine arteries, the mean PI values during the menstrual cycle was not significant difference. In the ovarian arteries, the mean PI values during the menstrual cycle was significant difference(p$lt;0.05). This study suggests that doppler velocity of the uterine and ovarian arteries during the menstrual cycle may help us to better understand the uterine and ovarian response to endocrine changes.