제주도 연안 해수의 화학적 및 미생물학적 수질 현황에 관한 연구

김재하,박길순,강영주 제주대학교 해양연구소 1981 해양자원연구소연구보고 Vol.5 No.-

A survey of seawater pollution was carried out monthly during October 1979 through May 1980 for three major harbors in Jeju island ; Jeju, Seogwipo and Seogsanpo. Eight different sites were selected for each harbor to determine a chemical and microbiological pollution level together with a pigment content. Among three harbors, Jeju was most polluted chemically and microbiologically with significantly high level of pollution at the inner harbor which were well above the maximum permitted level. The inner harbor of Seogwipo also was in excess of permitted level under the influence of Cheonjicheon, but the other sites were rather clean. Seongsanpo is not polluted at the present time. It was shown that the pigment contents in seawater were higly variable from month to month. The highest pigment content in Jeju with the similar low values in Seogwipo and Seongsanpo were obtained oqter port. This indicates that the sites with inflowing fresh water or sewage were and carotenoid. throughout the investigating period. The differences in the pigment contents among the sampling sites showed that the inner harbor was much higher than outer prot. This indicates that the sites with inflowing fresh water or sewage were generally high in pigment content. In pigment sorts, there always appeared higher amount of chlorophyll c than chlorophyll a,b and carotenoid.

유기용제에 장기간 폭로된 근로자의 정신의학적 기능과 삶의 질

김석재,강순아,유지숙,국승희,윤진상,문재동,이형영 大韓神經精神醫學會 2000 신경정신의학 Vol.39 No.5

연구목적: 본 연구는 Y공단의 제조공정에서 저농도의 유기용제(solvent)에 장기간 노출된 근로자의 정신의학적 기능과 삶의 질을 평가하고자 하였다. 방법: 총 142명의 남성근로자 중 유기용제에 폭로된 군(이하 폭로군) 82명과 폭로되지 않는 군(이하 비폭로군) 60명에게 간이정신진단검사(Symptom Checklist-90-R, SCL-90-R), 인지 및 정신운동성검사 (Critical Flicker Fusion Threshold. CFFT; Choice Reaction Time, CRT;Compensatory Tracking Task, CTT;Digit-Symbol Substitution, DDS) 및 한국판 스미스클라인 비챰 삶의 질 척도(Korean version of the SmithKline Beecham 'Quality of Life' Scale, KvSBQOL) 등을 적용하였다. 두 군간의 비교는 평가치의 평균에 대해 t-검증을 하였고 또한 연령과 학력을 공변인으로한 공변량분석(ANCOVA)을 하였다. 결과: SCL-90-R의 9개 증상 차원과 3개 전체 지표 점수는 두 군이 각각 정상범위였다. 그러나 신체화 (Somatization, SOM), 우울(Depression, DEP), 적대감(Hostility, HOS), 공포불안(Phobic anx-iety, PHOB)의 증상차원과 전체심도지수(Global Severity Index, GSI), 표출증상합계(Positive Symptom Total, PST)의 전체 지표는 폭로군이 비폭로군보다 유의하게 높았다. 인지 및 정신운동성 검사와 삶의 질은 두 군간에 유의한 차이는 없었다. 결론: 이상의 결과는 유기용제가 허용치 이하일지라도 장기간 폭로되는 경우에는 정신의학적 문제를 다소 일으킬 수 있음을 시사하였다. 중심단어:유기용제·근로자·정신건강·정신운동성 기능·삶의 질. Objectives: The aim of this study was to evaluate the psychiatric function and quality of life of the chemical workers who had been exposed to long-term, low-level organic solvents in the Y industrial complex. Method: A total of 144 male workers, of whom 82 were in the exposed group and 60 in the unexposed-control group, were evaluated by using Symptom Checklist-90-R(SCL-90-R), series of neuropsychological tests(critical flicker fusion threshold, CFFT) ; choice reaction time, CRT ; compensatory tracking task, CTT) ; digit symbol substitution test, DSS), and the Korean version of the SmithKline Beecham Quality Of Life scale(KvSBQOL). For the statistical analyses, comparisons of the means of the two groups were performed for each variable with two-tailed t-test as well as ANCOVA including age and education as covariates. Results: For the SCL-90-R, the nine symptom scales and the three global indices were clinically within normal range in both groups. However, the scores on the symptom scales of 'somatization','depression','hostility' and 'phobic anxiety', and those on the global indices of 'global severity index' and 'positive symptom total', were significantly higher in the exposed group than in the unexposed group. In terms of the neuropsychological function and the quality of life, there wes no significant difference between the two groups. Conclusion: These findings suggested that the long-term eposure to mixed organic solvents, even at the low level below the permissible exposure limit, might induce some psychiatric problems. KEY WORDS: Organic solvent·Worker·Mental health·Psychomotor function·Quality of life.

마우스에서 조합시험법에 의한 고려홍삼의 생식발생독성 평가

김미라, 박동선, 장자영, 신선희, 이예은, 백인정, 연정민, 이세라 , 남상섭, 황방연, 노재섭, 김순선, 조대현, 남상윤, 이범준, 김윤배, 윤영원 충북대학교 동물의학연구소 2006 Journal of Biomedical and Translational Research Vol.7 No.1

본문참조

한국인 집단의 효소의 다형현상에 대한 연구 : 태반과 혈액에서의 MDH, LDH 그리고 GPI에 대하여 MDH, LDH and GPI in Placental and Blood

鄭瑢載,姜順子,金甲英 이화여자대학교 생명과학연구소 1990 생명과학연구논문집 Vol.1 No.-

한국인 집단의 태반조직과 혈액 내에서의 Malate dehydrogenase(MDH), Lactate dehydrogenase(LDH) alc Glucose phosphate isomerase(GPI)에 대한 다형현상을 알아 보고자 horizontal starchgel 전기영동법을 이용하여 조사한 결과는 다음과 같다. 1. MDH 동위 효소는 혈액에서는 , 한가지 표현형, HDHs1이 나타났으며, 태반에서는 SolubleMDH와 Mitochondial-MDH의 두 form이 모두 나타나는데 태반조직의 MDHs locus에서는 MDHs1 유형만이 나타났고 MDHs locus에서도 보편적 유형인MDHm1 표현형이 나타났다. 2. LDH 동위 효소는 표현형이 혈액에서 normal LDH isozyme인 LDH1, LDH2, LDH3, isozyme이 나타났고, 태반에서는 LDH1(LDHB4) isozyme 한가지 유형만 나타났다. 3. GPI 동위 효소는 혈액과 태반에서 표현형이 모두 같은 형인 GPI1으로 나타났으며 변이형은 한국인 집단에서 발견되지 않았다. 4. 따라서 한국인 집단의 혈액과 태반에서 MDH, LDH, GPI 효소는 모두 monomorphic한 것으로 나타났으나 LDH의 경우만은 혈액과 태반에서 검출된 isozyme의 종류가 상이하였다. Genetic polymorphisms of three placental and blood cell enzymes malate dehydrogenase(MDH),lactate dehydrogenase(LDH) and glucose phosphate isomerase(GPI) in Korean population was studied by starch gel electrophoresis. The results obtained were as follows: 1. MDH phenotype was found to be only MDHs 1 in blood cells. Both of MDHs and MDHm were found in the placental. Only MDHs 1 was found at the MDHs I locus. 2. LDH phenotype was found to be the normal LDH isozyme-LDH1, LDH2 and LDH3 isozyme in blood cells. And DH1(LDHB4) isozyme was found only in the placental extracts. 3. GPI phenotype was found to be only GPI 1 in blood cells and the placental extracts, and no variant type was ound in Korean population. 4. Based on the above results, MDH, LDH and GPI isozymes are monomorphic in blood cells and placental extracts of Korean population.

한국인 집단의 혈액과 태반에서의 Carbonic anhydrase와 Esterase D의 다형현상에 관한 연구

鄭瑢載,姜順子,金惠蘭 梨花女子大學校 韓國生活科學硏究院 1990 韓國生活科學硏究院 論叢 Vol.45 No.-

한국인 집단의 혈액과 태반 조직내에서의 carbonic anhydrase Ⅰ(CAⅠ), carbonic anhydraseⅡ(CAⅡ) 및 esterase D(EsD)효소에 대한 다형현상을 horizontal starch gel 전기영동법을 이용하여 조사한 결과는 다음과 같다. 1. 혈액에서는 CAⅠ과 CAⅡ 동위효소는 각각 CAⅠ1과 CAⅡ1유형의 한가지 표현형만을 나타냈으며, 변이형은 한국인 집단에서 발견되지 않았다. 2. EsD 동위효소는 혈액과 태반에서 모두 EsD*1과 EsD*2의 2개 대립유전자가 발견되었으며 변이형은 발견되지 않았다. 3. 적혈구에서의 EsD좌위의 대립유전자 빈도는 EsD*1은 0.661, EsD*2는 0.339로 나타났다. 4. 태반에서 나타나는 EsD의 유전자 빈도는 EsD*1과 EsD*2가 각각 0.664와 0.336으로 나타났다. 5. 위의 결과를 근거로 한국인 집단에서의 CAⅠ, CAⅡ효소는 monomorphic하며 EsD는 polymorphic함을 알 수 있다. Genetic polymorphism of the placental and red blood cell enzyme, carbonic anhydrase 1(CA1), carbonic anhydrase 11(CA11) and esterase D(EsD) in korean population were examined by means of horizontal starch gel electrophoresis. The results obtained were as follows : 1. Both of the CA1 and CA11 phenotypes were found to be only CAⅠ*1 and CAⅡ*1, respectively. No variant type was found in Korean population. 2. Two alleles, EsD*1 and EsD*2 were found in blood cell and placental extract, respectively no variant type was found. 3. The gene frequencies of EsD alleles in blood cell were calculated to be 0.661 for EsD^*1 and 0.339 for EsD^*2. 4. The gene frequencies of EsD alleles in placental extract were calculated to be 0.664 for EsD^*1 and 0.336 for EsD^*2. 5. Based on the above results, both CAⅠ and CAⅡ isozymes are monomorphic and EsD isozymes is polymorphic in Korean population.

한국인 집단의 효소의 다형형상에 대한 연구 : 태반과 혈액에서의 MDH, LDH 그리고 GPI에 대하여 MDH, LDH and GPI in Placental and Blood

鄭瑢載,姜順子,金甲英 梨花女子大學校 韓國生活科學硏究院 1990 韓國生活科學硏究院 論叢 Vol.45 No.-

한국인 집단의 태반조직과 혈액 내에서의 Malate dehydrogenase(MDH), Lactate dehydrogenase(LDH) 및 Glucose phosphate isomerase(GPI)에 대한 다형현상을 알아 보고자 horizontal starch gel 전기영동법을 이용하여 조사한 결과는 다음과 같다. 1. MDH 동위 효소는 혈액에서는, 한가지 표현형, HDHs1이 나타났으며, 태반에서는 Soluble-MDH와 Mitochondrial-MDH의 두 form이 모두 나타나는데 태반조직의 MSHs locus에서는 MDHs1 유형만이 나타났고 MDHs locus에서도 보편적 유형인 MDHm1 표현형이 나타났다. 2. LDH 동위 효소는 효현형이 혈액에서 normal LDH isozyme인 LDH1, LDH2, LDH3, isozyme이 나타났고, 태반에서는 LDH1(LDHB4) isozyme 한가지 유형만 나타났다. 3. GPI 동위 효소는 혈액과 태반에서 표현형이 모두 같은 형인 GPI1으로 나타났으며 변이형은 한국인 집단에서 발견되지 않았다. 4. 따라서 한국인 집단의 혈액과 태반에서 MDH, LDH, GPI 효소는 모두 monomorphic한 것으로 나타났으나 LDH의 경우만은 혈액과 태반에서 검출된 isozyme의 종류가 상이하였다. Genetic polymorphisms of three placental and blood cell enzymes malate dehydrogenase(MDH), lactate dehydrogenase(LDH) and glucose phosphate isomerase(GPI) in Korean population was studied by starch gel electrophoresis. The results obtained were as follows : 1. MDH phenotype was found to be only MDH_2 1 in blood cells. Both of MDHs and MDHm were found in the placental. Only MDHs 1 was found at the MDHs I locus. 2. LDH phenotype was found to be the normal LDH isozyme-LDH1, LDH2 and LDH3 isozyme in blood cells. And LDH1(LDHB4) isozyme was found only in the placental extracts. 3. GP I phenotype was found to be only GP I1 in blood cells and the placental extracts, and no variant type was found in Korean population. 4. Based on the above results, MDH, LDH and GPI isozymes are monomorphic in blood cells and placental extracts of Korean population.

雪嶽山 森林植生의 植物現存量과 一次純生産力의 推定

任良宰,金泰旭,白順達 서울大學校 農科大學 1984 서울대농학연구지 Vol.9 No.2

雪嶽山 地域의 植生構造 및 機能을 把握하기 위하여 綠地自然度(degree of green naturalily, DGN), 一次純生産力과 植物現存量을 推定하였다. 綠地自然度는 地形圖(1:50,000)에 362개의 1km×1km의 mesh를 그은 다음 等級을 査定하였다. 總 362 mesh 중 DGN 8이 全體의 63.8%인 231 mesh를 占하였고 이는 自然林에 가까운 二次林이 大部分인 것을 意味한다. DGN 9인 自然林은 溫量指數에 依해 區分한 惡寒帶地域에 分布하였다. 一次純生産力은 麟蹄, 襄陽, 束草의 氣象資料를 反映하여 Miami model (Lieth 1972, 1973)에 의해 推定하였는 바 東(外)雪嶽, 西(外)雪嶽, 西雪嶽의 값은 各各 1,397, 1,541, 1,518 g/㎡/yr이었고 平均値는 1,455 g/㎡/gr이었다. 雪嶽山 全體의 年間 一次純生産量은 504,405,81 ton/yr이었으며 이를 實際面積으로 換算하면 約 55萬ton이 蓄積되어 가는 것이라 할 수 있다. 雪嶽山의 植物現存量은 25,075,974.8㎥이었으며 이는 다시 約 2,300,000 ton으로 推定할 수 있었다. 그러나 葉量이나 根量이 除外되어 있음을 考慮하면 3,000,000 ton 以上이 蓄積되어 있는 것으로 推定되며 이는 素(C)의 平均停滯 約 55年間인 것으로 推定된다. 한편 Choi & Yim (1984)에 依하면 雪嶽山의 5대 優占種의 順位는 신갈나무 소나무 당단풍나무 졸참나무 서어나무 順이었다. To evaluate the functional feature of Mt. Seolag area, the maps of degree of green naturality. (DGN) and net primary productivity and the estimation of total phytomass were made. DGN map was made by scoring the value of DGN rank in topography map (scale 1:25,000) divided into 362 meshes of Km?? size. In the map, the higher values in DGN rank were found at high elevation area, which is the subarctic zone determined by warmth index isopleth. Of total meshes, 231 meshes (63.8%) were the degree 8 in DGN rank which is secondary forest closed to natural forest vegetation. The net primary productivity was calculated by Miami model (Lieth 1972, 1973) using the data measured at Inje, Sogcho and Yangyang meteorological station . The estimated values of eastern, western and southern Seolag were 1,397, 1,451 and 1,518g/m²/yr, respectively, and the mean of them was 1,455/m²/yr. The amount of annual net primary production in the whole Mt. Seolag was 504,405.81tons/yr obtained by multiplication of net primary productivity and practical land area (plane area x mean inclination of 25 degree: 1/cosine20°). The mean 25°was the value obtained by the analysis of topographic map with 5535 meshes of 250m×250m size mesh. The Phytomass of whole Mt, Seolag area, 25,075,974.8m³, was estimated from the data of sample area (30,250m²). The Phytomass will be over 3,000,000 tons if it would be considered the phytomass increased by the increase of area (converted to practical area)and the one of leaf and root system. It means the accumulated amount of C for 55 years. On the other hand, according to choi & Yim (1984), Quercus mongolica, Pinus densiflora, Acer pseudosieboldianum Quercus serrata, Carpinus laxiflora etc. Were found as dominant species in order of species sequence of Mt, Seolag.

항-E 항체에 의한 급성 신부전을 동반한 지연성 용혈성 수혈 부작용 1례

강문수,변재원,윤창옥,김인순 大韓輸血學會 1999 大韓輸血學會誌 Vol.10 No.2

Rotavirus 전기영동형별을 위한 RNA 추출과정 및 조건의 비교연구

조은경,김은순,양재명,김경희,조양자 한양대학교 의과대학 1992 한양의대 학술지 Vol.12 No.2

Rotaviruses (Rv) are the major cause of severe dehydrating dearrhea among young children in Korea, where they are frequently responsible for 68% of the cases requiring hospitalization. Laboratory diagnosis of rotaviral infection in Korea depends mainly on enzyme-linked immunosorbent assay (ELISA) that employs expensive commercial diagnostic kits purchased from abroad and that shous the presence or absence of group A Rv only. PACE have however provided epidemiologic evidence to demonstrate the sxistence of different strains (both group A and atypical Rv) during diarrgeal outbreak, the appearance of new strains and desappearance of old ones from a community, shifts in the prenalence of strains, and the persistance of a single strain in newborns. For such reasons, we have felt that it is essential to raise the sensitivity and shorten the processing time for PAGE. We therfore have devised a rapid method for Rv RNA extration by directly extracting the Rv RNA from the specimens (that is, skipping freon extraction and running as ultracectrifuge) and using an urea-containing dissociation mixture to dissolve dried RNA for PAGE. We have also shorten the PAGE diagnostic results by sequential staining of the same gel with ethidium bromide and silver stain. With the use of the rapid method, it was feasible to increase the efficacy of the PAGE with reduced cost and working time. Phenol extration of stools, however, was essential in maintaining the sensitivity of the method.

골프장의 잔디 토양에서 살균제 Tolclofosmethyl의 분해

정근욱,우선희,김흥태,사동민,김영기,홍순달,김재정,이재구 한국환경농학회 2004 한국환경농학회지 Vol.23 No.3

Tolclofosmethyl (TCFM) is heavily and annually applied to the turf soils of most golf courses in Gyeongju to control the fungi known to cause the disease brown patch. The soil samples used for the experiment was collected three weeks after the annual application at the end of May in the year 2002. The preliminary results obtained from this study demonstrated that the repeated field annual applications of TCFM to the turf soils of a golf course located in Gyeongju city in the southern area of Korea showed the enhanced degradation of the parent compound TCFM, especially in the surface (0~15 cm) soil rather than the shallow subsurface (15~30 cm) and deep subsurface (30~45 cm) soils, compared to the corresponding surface (0~15 cm) and shallow and deep subsurface (15~30 cm and 30~45 cm) soils of the untreated plot. It appears that microorganisms in the soil may be involved in the enhanced degradation of TCFM.