A Cytotoxic Component from Angelicae Koreanae Radix against L1210 and HL-60 Cells

Bae, KI-Hawan,Ji, Jong-Myung,Kang, Jong-Seong,Ahn, Byung-Zun The Pharmaceutical Society of Korea 1994 Archives of Pharmacal Research Vol.17 No.1

A cytotoxic sesquiterpene against L1210 and Hl-60 cells was isolated from Angelicae Koreanae Radix (bulk-kang-hwal). The component was identified as bisabolangelone by means of chemical and physical methods. The $ED_{50}$ values of it were $1.20{\;}\mu\textrm{g}/ml$ against L1210 cells and $2.30{\;}\mu\textrm{g}/ml$ against HL-60 cells. Bisabolangelone was found in bulk-kang-hwal but not in kang-hwal.

발효조를 이용한 Monascus anka의 적색소와 황색소의 생산

강성국,임종환,정순택,김선재 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.6

Monascus anka를 이용하여 천연식용색소를 대량생산하기 위한 방법을 개발하기 위하여 발효조를 이용하여 적색소와 황색소의 색소생산의 최적 배양 조건과 생산된 색소의 색깔 특성을 조사하였다. Monascus anka 적색소와 황색소를 생산하였는데, 이들 색소는 균체내색소와 균체외 색소로 구분되었다. 균체의 적색소(ERP)는 494nm에서, 균체외 황색소(EYP)는 380nm에서, 균체내 적색소(IRP)는 506nm에서 그리고 균체내 황색소(IYP)는 388nm에서 최대 흡광도를 나타냈다. 적색소와 황색소 생산, 색조의 특성 및 균체증식력을 배양 온도, pH, 쌀가루 농도, peptone 농도, magnesium sulfate 농도, 통기량 및 교반속도에 대하여 조사한 결과 적색소 생산력은 30℃, 초기 pH 6.0, 쌀가루 농도 3~5%, peptone 농도 0.05%, magnesium sulfate 농도 0.25%, 통기량 0.1vvm 교반속도 300rpm의 조건에서 최대치를 보였으며, 이때 ERP, EYP, IRP 및 IYP는 각각 A_494nm 0.84, A_380 nm 0.71, A_506 nm 1.18, A_388 nm 1.10, L값은 각각 29.40, 6.44, 34,98, 8.94, a값은 각각 5.76, -1.08, 11.83, -1.55, b값은 각각 18-19, 3.41 27.26, 6.16 그리고 균체량은 7.4 g/l을 보였다. 또한 황색소 생산력은 온도의 경우 적색소 생산을 위한 최적온도 보다 높은 35℃ 부근에서 우수하였으며 초기 pH와 쌀가루 농도는 적색소 생산을 위한 최적조건과 같았고, 질소원과 무기염의 농도가 높을수록 그리고 통기량이 많을수록 황색소 생산력이 우수하였다. 적색소와 황색소 생산을 위한 발효조의 교반속도는 100~300 rpm이 적합하였다. Production of Red and Yellow Pigments from Monascus anda in a Jar Fermenter, Seong-Gook Kang, Jong-Whan Rhim, Soon-Teck Jung^* and Sun-Jae Kim. Department of Food Engineering. Mokpo National University Chonnam, 534-729, Korea - In order to develop the method for mass production of natural food colorant from Monascus anka, optimum cultivation conditions for producing red and yellow pigments by cultivating the mold in a jar fermenter and their color characteristics were investigated. The mold produced red and yellow pigments both intracellularly and extracellularly. These pigments showed unique light absorption characteristics with maximum absorption of 494, 380, 506, and 388 nm for extracellular red pigment (ERP), extracellular yellow pigment (EYP), intracellular red pigment (IRP), and intracellular yellow pigment (IYP), respectively. Optimum conditions for producing red pigments were found to be temperature 30℃, initial pH 6.0, rice powder 3~5%, peptone 0.05%, magnesium sulfate 0.25%, aeration rate 0.1vvm. Optimum temperature for producing yellow pigments was around 35℃ which is higher than that of producing red pigments. The initial pH rice powder concentration for producing yellow pigments were the same as those of producing red pigments. The higher concentration of nitrogen source and inorganic salt, aeration rate, the more the yellow pigments were produced. Them optimum agitation speed was 100~300 rpm for pigment production.

Effects of dietary supplementation with probiotic CS-A on performance in broiler chickens

Seong Soo Kang1*, Se Eun Kim, Ara Go, Kyung Mi Shim, Chun Sik Bae, Chang Jong Moon, Sung-Ho Kim, Jong-Choon Kim, Jin-Cheol Yoo, Seung Sik Cho 충북대학교 동물의학연구소 2012 Journal of Biomedical and Translational Research Vol.13 No.3

Probiotics, enzymes, organic acids, oligosaccharides, antioxidants, and other functional materials are actively being explored as alternatives to antibiotics. Probiotics include live beneficial microorganisms that colonize the intestinal tract and competitively inhibit attachment and growth of harmful microbes. Probiotics also increase feed efficiency by assisting in nutrient absorption and digestion. The current study was conducted in order to evaluate the effect of a new probiotic, CS-A, as a dietary supplement of a fermented product on growth performance, feed intake, and feed conversion efficiency in broiler chickens, and to evaluate its value as an alternative for antibiotics used as a feed additive. Antibacterial and anti-inflammatory effects of CS-A were investigated in vitro and the in vivo effects of a constant concentration of supplemented CS-A on growth rate and feed efficiency were evaluated. In addition, the safety of CS-A was assessed by examination of common symptoms and mortality. Determination of minimal inhibitory concentration revealed an excellent antibacterial effect of CS-A. Cytotoxicity was low and anti-inflammatory effects were achieved at the effective concentration of CS-A. Supplementation with 0.1% CS-A resulted in a feed efficiency score of 1.84 in broilers, compared to 2.00 in the control group. There were no adverse clinical findings, necropsy findings, hematology, and altered serum biochemistry parameters, and no mortality. Thus, it is concluded that CS-A is safe and effective as a feed additive.

우리나라 지역 직업성질환 감시체계의 현황과 전망

임종한,장성실,김성아,문재동,채창호,홍윤철,김수영,김진석,김영욱,한상환,이혜숙,원종욱,송동빈,하은희,강성규 대한산업의학회 2001 대한직업환경의학회지 Vol.13 No.2

기존의 특수건강진단과 작업환경측정을 통한 직업병 관리가 진폐증, 소음성난청 등의 소수 특정질환에 국한되고 실제 직업병 발생 규모 파악이나 신종 직업병의 발견에 한계를 보인다는 사실은 산업의학전문가들 사이에서도 공감을 이루고 있다. 미국과 영국 등에서의 직업성질환 감시체계에 대한 경험은 우리 나라의 직업성질환 감시체계 구축에도 새로운 자극제가 되면서, 1998년이후 인천, 대전, 여천, 구미, 부울경 지역에서 지역 직업성질환감시체계를 산업보건관리의 중요한 시스템으로 구축하려는 노력이 확산되고 있다. 새로이 구축되어지는 이들 지역 직업성질환 감시체계는 감시하고자 하는 대상질환, 활용 가능한 인적자원 및 자료원, 지역 의료체계의 특수성 등에 따라서 목적과 방법을 달리하면서 독특한 형태로 발전을 하고 있다. 각 지역단위 감시체계들이 그 상황에 맞게 독특한 목적과 전략들을 발전 시키면서도, 향후 발전할 국가적인 차원의 직업성질환 감시체계 구축을 위하여 직업성질환 감시의 기본 전략 등을 공유하는 등의 노력이 필요하다. 환례 정의 및 기본적인 등록 서식의 공유, 직업성질환 감시 자료원의 발굴, 공동의 정보 네트워크 및 직업성질환 감시 데이터베이스 구축 등 직업성질환 감시활동을 지원하기 위한 여러 기초 인프라 구축에 힘을 모아야 할 것이다. 우리 나라에서 직업성질환 감시체계를 성공적으로 구축하기 위해서는 수집된 자료의 질 관리를 위한 직업성질환 감시의 원칙 제정과 감시 전략의 공유 등이 필요하며, 전국적인 직업성질환 감시체계의 하부구조라고 할 수 있는 지역감시체계의 기초 토대 마련과 강화작업이 절실하게 필요하다.

HPLC-ECD를 이용한 흰쥐의 뇌 중 감마아미노부티르산 및 글루탐산의 정량

강종성,이순철 충남대학교 약학대학 의약품개발연구소 1999 藥學論文集 Vol.15 No.-

A sensitive and efficient assay method was applied to determine the level of glutamic acid (GA) and γ-aminobutyric acid (GABA) in frontal cortex and hippocampus of rat administrated with ethanol and drugs. The compounds were derivatized with ο-phthalaldehyde (OPA) and 2-mercaptoethanol for precolumn analysis. The condition for the simultaneous determination of GA, GABA and beta-aminobutyric acid (BABA) by high performance liquid chromatography with electrochemical detection was reverse phase C_18 column as stationary phase, 0.1M phosphate buffer containing 0.1mM Na_4EDTA: methanol=55:45 (v+v) pH 3.8 as mobile phase and 0.7V electrode voltage. The stability of reaction product of GA, GABA and BABA with OPA could be increased by adding the same volume of polyethylene glycol 400 to reaction mixture. The GABA level in frontal cortex of rat was significantly decreased by the administration of picrotoxin and diazepam, but it was significantly increased by the administration of red ginseng total saponin, N-methyl-D-glucamine and (-)-deprenyl.

전기절연유의 절연파괴에 미치는 전극간격 및 곡률반경의 영향

강성화,채홍인,이종필,임기조 한국안전학회 2004 한국안전학회지 Vol.19 No.1

In this paper, we investigated the influence of gap length and tip radius on breakdown of mineral based insulation oil. Applied voltages were DC and AC voltage. Electrode system was needle-plane structure. The tip radius of needle electrode was 5, 10, 20 and 25μm, respectively. We measured breakdown voltage for each of tip radius with increasing electrode gap, 2mm to 12mm. Electric breakdown strength at tip was calculated using Mason's equation contained geometric figure. As gap length increased, breakdown strength increased linearly. But, as tip radius of needle increased, breakdown strength decreased exponentially. It can be explained by the phenomenon that electron is easily injected, as tip radius increases, and effective work function decreases. When appling DC voltage, breakdown strength was higher when polarity of needle was negative than positive. It is because of the space charge effect in accordance with the influence of liquid motion.

두경부 종양세포주에 대한 Mithramycin A의 세포자멸사 유도성 및Cisplatin 항암작용 증가효과

이강대,손성환,박종욱,김영록,이봉주,김주연 고신대학교의과대학 2008 고신대학교 의과대학 학술지 Vol.23 No.1

Background and Objectives : Cisplatin (CP) is a major chemotherapeutic agent for head and neck cancer. However, cancer cells can get drug resistance through recovering the DNA damage by CP. Mithramycin A (MA) can inhibit gene expression of multidrug resistance gene 1, c-myc or h-ras. The goal of this study is to evaluate the therapeutic effect of MA alone and combination of MA and CP on head and neck cancer cell lines. Materials and Methods : Two head and neck cancer cell lines (AMC-HN4, AMC-HN7) were treated with MA. The cellular proliferation, apoptosis and protein expression were measured with XTT assay, FACS analysis, and western blotting, respectively after MA treatment in cell lines. In order to know the combination effect of MA with CP, the cancer cell lines were treated with MA, CP, and MA/CP combination, and cellular proliferation, apoptosis and expression of apoptosis-associated proteins were measured. Results : MA inhibited the proliferation of head and neck cancer cells and induce dapoptosis. Increase of caspase activation, PLC-?1 fragmentation and percent apoptosis by MA treatment was blocked by z-VAD-fms, but not blocked by N-Acetyl Cystein. Combination of MA and CP significantly inhibited cancer cell growth and increased apoptosis compared to MA and CP alone, and it also suppressed the expression of anti-apoptotic proteins such as XIAP and Mcl-1. Conclusions : The present study suggests that MA alone andMA/CP combination chemotherapy could be a potential regiment for the control of the head and neck cancer.

자기베어링시스템 강성의 실험적 고찰을 통한 안정화 특성에 관한 연구

강성구,강종규,홍준희 한국공작기계학회 2004 한국공작기계학회 추계학술대회논문집 Vol.2004 No.-

Due to the lack of stiffness and difficulties of control, it is hard to achieve well balanced magnetic levitation. In this paper, we analysis the current and position stiffness change according to bias current through experiment. Then, compensation equations were presented. After obtaining PD gain for each bias current and PD gain region through levitation experiment, we consider the characteristics.

글래스비드 방법과 분말프레스 방법을 이용한 지르코니아 분말시편의 반정량 엑스선형광분석

강종봉,조범래,양성구 경남대학교 신소재연구소 1999 論文集 Vol.11 No.-

XRF는 시편의 종류에 따라 벌크, 분말 등 여러 가지 형태의 시료를 간단한 전처리 조작에 의해 빠른 시간에 분석할 수 있다는 장점으로 파인세라믹스의 원소분석으로 이용되고 있다. 그러나 시편들의 전처리 방법에 따라 분석의 결과가 달리 나타나고 있어, 본 실험험에서는 분말프페스형 시편과 글래스비드형 시편에서의 분석결과 차이를 밝히고자 하였으며, 분석결과에 미치는 영향들을 살펴보고자 하였다. 글래스비드형 시편인 경우 첨가하는 10배 이상의 플럭스 때문에 측정시편에 포함된 실제 분석하고자하는 시료의 양이 적어 오차의 범위가 크게 나타났다. 미량원소의 분석일 경우 글래스비드형 시편이 분말프레스형 시편의 시편보다 측정결과에서 많은 오차를 나타낸다. 이것은 X-선이 조사되는 영역에서 실제 시편내의 시료의 양이 미량 원소 분석의 오차를 나타냄을 알 수 있었다. XRF(X-ray Fluorescence Spectrometer) is one of the element analyzing instruments. which can be used to analyze ceramic materials of bulk, powder and various forms. The advantage of XRF is rapid analysis of elements in ceramics with simple sample preparation. In this study, the difference in results between pressed powder method and glass bead method were evaluated and the effects of sample preparation the final results were examined. The flux included in glass bead method, which is more than 10 times of samples, affected the final analyzing result. Trace element analysis in XRF could be affected by the sample amount because the region of X-ray beam is related the sample amount.

HPLC-ECD를 이용한 흰쥐 뇌의 선조체 중 신경전달물질의 동시분석시 최적 조건

강종성,문민선,신형선,이순철 충남대학교 약학대학 의약품개발연구소 1995 藥學論文集 Vol.11 No.-

흰쥐 뇌의 선조체로부터 catecholamine, indoleamine 및 대사산물을 동시분석할 수 있는 간편한 방법을 제시하였다. 기기는 ECD를 장착한 HPCL를 사용하였고, 고정상인 C_18 컬럼에서, 이동상은 10mM citric acid, 0.13mM Na_4EDTA, 0.58mM SOS를 함유하는 35mM sodium acetate buffer(pH 3.4) : MeOH=85:15가 최적이었다. 이 때 컬럼온도는 30℃였다. 이 조건으로 dopamine(DA), 3, 4 -dihydroxyphenyl acetic acid(DOPAC), homovanilic acid(HVA), 5-hydroxyindole acetic acid(5-HIAA), serotonin(5-HT) 및 noradrenaline(NA)을 미량까지 분석할 수 있었고, 검출한계는 각 성분에 대해 1회 주입당 2~10pg이었다. 이 방법으로 흰쥐의 주령 및 성별에 따른 선조체내 생체아민과 대사산물의 함량을 비교하였다. 7주령 쥐에서 암컷은 수컷보다 DA와 5-HT의 함량이 유의성 있게 높은 것으로 나타났으며, 주령이 증가함에 따라 암컷에서 DOPAC의 함량이 유의적으로 증가하였다. A simple, efficient and sensitive method was described for the simultaneous determination of catecholamine, indoleamine and related metabolites from the homogenates of the rat brain striatum by HPLC-ECD. The optimum mobile phase on a reverse phase C_18 column was 35mM sodium acetate buffer(included 10mM citric acid, 0.13mM Na_4EDTA, 0.58mM SOS, pH 3·4):MeOH=85:15. The column temperature was 30℃. Dopamine(DA), 3, 4-dihydroxyphenyl acetic acid(DOPAC), homovanilic acid(HVA), 5-hydroxyindole acetic acid(5-HIAA), serotonin(5-HT) and noradrenaline(NA) could be separated and analysed to very small amount. The detection limits of this method were 2~10pg per injection for all components. The effects of age and sex of rat on the contents of the catecholamines and their metabolites in rat brain striatum were studied. The levels of DA and 5-HT contents of the 7 week old female rats were higher than those of the 7 week old male rats. As the age of rat increases, the contents of DOPAC increased significantly.