김치에서 분리한 Lactobacillus sp. JC-7과 Lactobacillus acidophilus 88간의 Electrofusion 최적조건 설정

조영배,최현정,백형석,전홍기 부산대학교 유전공학연구소 1997 분자생물학 연구보 Vol.13 No.-

김치 발효 숙성기간을 연장하고 신선도를 오랫동안 유지 할 수 있는 김치발효 starter의 개발을 목적으로 최적숙성기에 있는 김치로부터 bacteriocin생성능이 없는 유산균을 분리하여 동정하였으며, bacteriocin생성능이 우수한 L.acidophilus 88을 융합시키기 위해 electrofusion에 대한 최적 조건을 검토하였다. 분리균주는 Lactobacillus속으로 동정되어 Lactobacillus sp. JC-7이라 명명하였다. Electrofusion에 의해 생성된 융합주를 식별하기 위해 streptomycin(2.5㎎/ml)에 내성을 나타내는 Lactobacillus sp. JC-7변이주와 kanamycin(600㎍/ml)에 내성을 나타내는 L.acidophilus 88변이주를 분리하였다. Electrofusion을 100V/㎝, 120msec(72ohms,1670 capacitance) 에서 수행했을 때 융합효율이 가장 양호하였으며 전기장의 세기와 시간이 중가할수록 융합 효율이 현저히 감소하였다. 2가 양이온은 농도가 중가할수록 대체적으로 융합효율을 감소시키는 경향을 나타내었으나 1mM MGCl_2에서는 대조군에 비해 융합효율이 약간 증가하였다. PEG매개에 의한 융합법의 융합효율을 비교한 결과, 융합효율은 chemical fusion<electrofusion<electrofusion+20% PEG순을 나타내었다. Evaluation of Optimum Conditions for the Electrofusion between Lactobacillus sp. JC-7 Isolated from Kimchi and Lactobacillus acidophilus 88. Young-Bae Jo, Hyun-Jung Choi, Hyung-Suk Baik and Hong-Ki Jun*. Department of Microbiology, Pusan National University, Pusan 609-735,Korea-A lactic acid bacterium was isolated from kimchi. The isolated strain was identified as the genus Lactobacillus thrugh its morphological characteristics and named as Lactobacillus sp. JC-7. The optimum conditions for the electrofusion between streptomycin(2.5㎎/ml)resistant mutant of Lactobacillus acidophilus 88 and kanamycin(600㎍/ml) resistant mutant Lactobacillus sp. JC-7 were evaluated. The highest number of fusants were obtained at a capacitance value of 120msec(1670㎌), a field strength of 100V/㎝,and a pulse controller setting of 72Ω. The potimum pH of elecroporation buffer was 7.5 and the concentration of divalent cation was 1mM MG^2+. Electrofusants were efficiently obtained by addition 20% polyethylene glycol to electroporation buffer. The yield of fusion was better than that of using polyethylene glycol mediated chemical induction.

前腕部 經穴 取穴에서 骨度分寸法과 一夫法의 比較 硏究

박히준,채윤병,차웅석,박종배,이혜정,이향숙,인창식,고형균,김수영,최일환,김강식,문정배,배기태,유경환,육근영,정병주,손인철,임사비나 WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 2004 東西醫學硏究所 論文集 Vol.2004 No.-

Background : The cun measurement System, an essential and convenient method in locating acupoints, has been widely used in the practice of acupuncture. However, traditional cun measurement has been criticized for its lack of reliability. Objectives : The purposes of this study are to determine if one cun measured by the directional methods have a consistency with that of proportional methods and to investigate which factors are related with these differences, especially in forearm. Methods : The distance between the elbow crease and the wrist crease of forearm was compared to a reference value of one cun obtained by the directional method. In this method, one cun is one third of the distance between index finger and Small finger of a subject, measured at proximal interphalangeal joint. In addition, to investigate the factors influencing the differences between these two methods, we measured the height and body weight and calculated body mass index (BMI). Finally we analyzed the factors correlated with these lengths by linear regression test. Results : The results showed that one cun obtained by the directional methods were significantly different from one cun by the proportional methods in forearm. It was demonstrated that the length acquired with the directional method was more correlated with body weight and body mass index, while the length obtained by the proportional method was more correlated with the height.

GC 및 활성탄에 의한 알킬 치환 페놀류의 분자면적 추정

배선영,안형신,김건,이동선 서울여자대학교 자연과학연구소 1996 자연과학연구논문집 Vol.7 No.-

Activated carbons were prepared from leaves of Ginkgo biloba by activation process with zinc chloride after carbonization. The specific surface area of this activated carbon measured by BET surface analyzer was obtained as 469.8㎡/g. Adsorption of alkyl substituted phenols on activated carbon was determined using gas chromatography. It was found that molecular area could be estimated using Langmuir's isotherm, specific surface area, and Avogadro's number. Molecular areas for phenol, 2-ethyl phenol, 2,5-dimethyl phenol, 2-isopropyl phenol were 0.0288n㎡, 0.0796n㎡, 0.0384n㎡, and 0.1182n㎡, respectively. This estimation techniques are potentially useful to obtain molecular area for various compounds.

설치장소에 따른 태양열 스털링엔진 발전시스템의 성능예측에 관한 연구

배명환,장형성,강상율 경상대학교 생산기술연구소 2001 工學硏究院論文集 Vol.1 No.-

The simulation analyses of a dish solar power system with stilling engine in this study are applied to system performance prediction in four different test sites; Seoul, Pusan and Cheju in Korea, and Naha in Japan. The effects of difference of concentrator type such as monolithic and stretched-membrane construction on system efficiency are also evaluated. The total amount of generated power for a year depends on the site. However the total maximum system efficiency in every site is approximately 16% and there isn't a striking difference. It is also found that the maximum collector efficiency of stretched-membrane concentrator Is about 3 ∼ 15% lower than that of the monolithic type.

내용기반 분류에 의한 비디오 검색 시스템 설계

배종식,남윤성,양동일,최형진 강원대학교 정보통신연구소 2006 정보통신논문지 Vol.10 No.-

We suggest how to search news data by dividing structurely, by classifying meaningly, and by itemizing them by contents. The structural division divides the scene using correlative information between frames such as the distribution of the space brightness and the irregularity of brightness, and time relation etc. The meaning classification means to classify the detailed contents of news video as articles comparing special information extracted frame key frame with previous knowledge information. This study classifies the articles as the anchor's scene and the non-anchor's scene using the character that the progress of news is repeated centering around the anchor frames by periods. We get the non-anchor's scene to search the news data itemizing speech scene, interview scene and general scene, and classifying as articles. Also, we use the function of searching for summary by news icons and search the caption by processing the integration of caption, and design them to be able to search the news video quickly classifying news video as contents and indexing them.

방실차단 및 심실내전도장애에 관한 통계적 고찰

배형준 대한임상병리사협회 1994 대한임상병리사회지 Vol.26 No.1

A statistical study was done on atrioventricular block and intraventricular conduction disturbance in 127 cases taken on 1,761 patients subjected to electrocardiographic examination. The results were as follows 1. Total incidence of conduction disturbance was 7.21%, atrioventricular block was 2.67% and intraventricular block was 4.54% of total patients. Among of these, first degree atrioventricular block was 2.44% which was the most common occurred one, and complete right bundle branch block(2.33%), incomplete right bundle branch block (1.13%), left anterior hemiblock(0.74%), third degree atrioventricular block and left bundle branch block(each 0.17%), bifascicular block(0.11%), second degree atrioventricular block and left posterior hemiblock(erach 0.06%) in the order of frequency. 2. The ratio of male to female was 1.2:1 for atrioventricular block and 1.4:1 for intraventricular block. Atrioventricular block and intraventricular block were both common in over fifth decade. 3. The abnormal ECG findings wjth first degree atrioventricular block were sinus bradycardia(27.3%), left ventricular hypertrophy(21.2%), atrial enlargement(12.2%). In third degree atrioventricular block, the majority(50.0%) showed atrioventricular junctional escape rhythm. In complete right bundle branch block, there were 22.8% of atrial fibrillation and 14.3% of myocardial infarction. In incomplete right bundle branch block, there were 29.4% of both atrial enlargement and atrial fibrillation. In left anterior hemi-block. there was 21.5% of sinus bradycardia.

골격성 Ⅱ급 부정교합자의 악교정 수술 후 설골의 위치와 상기도 변화에 관한 연구

배진성,김경호,박형식,허종기,박광호 大韓顎顔面成形再建外科學會 2001 Maxillofacial Plastic Reconstructive Surgery Vol.23 No.6

OSAS(obstructive sleep apnea syndrome) is becoming more and more of importance nowadays. Therefore the study concerning OSAS is very important. Most of the studies which have been reported were comparison of upper airway size and change of skeletal class Ⅰ group and skeletal class Ⅲ group respectively. But, most of the showing OSAS have pharyngeal narrowing combined with more or less distinct maxillary and mandibular deficiency. Therefore the aim of this study is to see posterior airway space and hyoid bone position in patients affected by class Ⅱ malocclusion and treated with orthognathic surgery. we measured the lines between selected upper air way landmarks on lateral cephalometric x-ray films of skeletal class Ⅱ 30 persons who had not been operated yet, were within 2 weeks after operation, were 6 months after operation. And we compared them respectively and analyzed them with pairing t-test, pearson correlation coefficient and multiple regression analysis. The results were as follows. First of all, skeletal class Ⅱ group was increased in all the level of PAS, especially PAS(MK) and PAS related to hyoid bone(H-phW, H-PhW(Me-H)) was significantly decreased within 6 months after operation but that was larger than preoperation. And then the PAS was increased after mandibular advancement surgery. While there was no significant correlation between PAS and surgical methods, there was statistical significance between PAS and a number of surgical movement. At last, the hyoid bone was advanced within 2 weeks after operation but relapsed within the follow up period. In the end of the study, it was turned out that PAS was always wider without distinction of the region after the operation and that there was significant correlation between the moving volume of hard tissues and the changing quantity of PAS( ; how much PAS was changed according to the moving degree of hard tissues). After this, to be based on the study, I consider that OSAS demands further study and that it is necessary to do more practical applications to the patients.

답차운동부하검사법에 의한 관상동맥질환의 심전도학적 연구

배형준,김주옥 서울保健大學 1996 論文集 Vol.16 No.1

We examined adult patient l,000cases be questionable as coronary artery disease by treadmill exercise test, and the following results were obtained. 1. Total positive rate was 10.1%, and it was significantly differ between male and female(p<.05), and it was significantly differ as increase of age(p<.00l). 2. The stop reasons of treadmill exercise test were significantly differ between male and female, because of chest pain of female higher than male (p<.00l), and it was significantly differ as increase of age(p<.05), and it was significantly differ between positive and negative groups, because of most frequent stop reaseons were chest pain in the positive group and dyspnea in the negative group(p<.001). 3. Average maximum workload level of male was significantly higher than female(p<.001), and it was significantly decresed as increase of age(p<.001), and it was significantly differ between the total positive groups(81±2.7METS) and negative groups(9.3±2.4METS). And performance degree of maximum workload level in the positive group was most frequent as stage 3(42.6%). 4. Average maximum heart rate was not significantly differ between sexual groups in the positive group, but it was significantly differ between sexual groups in the negative groups(p<.001), and it was significantly decrease-as increase of age(p<.001), and it was differ between the total positive groups(158.5±23.0bpm) and negative groups(162.7±20.5bpm)(p<.0.1). 5. The reaching level of target maximum heart rate was not significantly differ as between sexual groups, as between age groups in the positive groups, but it was significantly differ in the negative groups, and it was not significantly differ between the total positive groups (93.2±l1.6%) and negative groups (94.2.±l0.l%). 6. Decreased cases of systolic blood pressure were 28cases in the toal positive groups, and most decreased as stage 2, and most frequently decreased level was lower than l0mmHg. 7. Decreased cases of ST segment were 93cases in the total positive groups, most frequently decreased level was higher than 1 mm under the baseline.

石彫에 있어서 石質에 따른 表現技法의 硏究

裵亨植 圓光大學校 1982 論文集 Vol.16 No.2

Stone-engraving, like wood-engraving, is the process of sculpture which is formed by engraving in manufacturing process of sculpture, characteristic technology is formed by experience and profound interest in materials, and it is necessary to continue the attitude of endurance. As the stone is various in species and nature, and the materials itself affect the character of a work, proper materials must be selected by sufficient tests considering with the expressive form, material and color. The moulding intension of artist must be expressed on materials without any troubles. The aim of research is to use stone through making a thorough analysis to fundamental nature, features and tools of stone, and to create a more beatiful sculpture through recognizing space, volume, material, color and movement which can be involved int he fundamental principles in directiton of stone-engraving.

병원내 생리기능 검사실의 적정 업무환경에 관한 연구

배형준,윤기은,김승곤,나동진,문희주,김태전,박정오 서울보건대학 1999 서울보건대학 부설 병원경영연구소 논문집 Vol.4 No.2

The purpose of this study was to suggest guideline for optimal work environment of physiological laboratory in hospital. Therefore this study was carried out to analyze the actual condition of laboratory system, major equipments and persons. The period of survey was from September to November 1997 and the objects were 123 laboratory. The major results of this study were as follows. 1. The optimal areas of general ECG room, exercise ECG room and echocardiology room were all 9-18㎡, that of Holter ECG room was 3-12㎡. The optimal areas of neurosystem laboratory were all 9-12㎡, those of respiratory system laboratory were all 9-18㎡. 2. The optimal number of persons laboratory of hospital 500 beds and less were 2∼3, those of 501∼700 beds were 3∼4.701∼1.000 beds were 4∼5 and over 1.001 beds were 7∼8 persons in circulatory system laboratory. The optimal number of persons laboratory of hospital 500 beds less than were 1∼2, those of 501∼700 beds were 3∼4, 701∼1.000 beds were 6∼7 and over 1.001 beds were over 8 persons in neurosystem laboratory. The optimal number of persons laboratory of hospital 500 beds and less 1. those 501∼1.000 beds were 1∼2 and over 1.001 beds were 2∼3 persons in repiratory system laboratory. 3. The optimal number of equipments of general ECG were 2∼3 in the 500∼1.000 beds hospital. there were 3∼4 units in the 1,001 beds. The optimal number of equipment EEG were both 1∼2 in the hospital 500 beds and less and 501∼700 beds. those were 4∼5 units in hospital over 701 beds. The optimal number of equipments of spirograph were both 1∼2 in the hospital 500 and less and 501∼1.000 beds. those were 2∼3 units in the over 1.001 beds. 4. In view of speciality and legal problem of physiofunctional test. we sugget that non-licensee were so quickly change place licensee in the interests of optimal work environment. 5. We suggest that to upgrade qualitative level of domestic equipment and to the more amplify utilization with respect to domestic equipment for the purpose of reducing loss of foreign money.