Laser scanning confocal microscope (LSCM)-fluorescence spectral properties of Nile Red embedded in polystyrene film of different thickness

Kim, Hyong-Ha,Song, Nam Woong,Park, Tae Sook,Yoon, Minjoong Elsevier 2006 Chemical physics letters Vol.432 No.1

<P><B>Graphical abstract</B></P><P>The ensemble and single molecule fluorescence spectral properties of Nile Red embedded in polystyrene (PS) films on glasses were investigated. Two distinct environments exist in the thin PS films on glasses: a low polarity environment in PS bulk and a high polarity environment near the PS/glass interface.</P><ce:figure id='figure.0010'></ce:figure> <P><B>Abstract</B></P><P>The ensemble and single molecule (SM) fluorescence properties of Nile Red embedded in polystyrene (PS) films on glasses were investigated. The ensemble spectra showed dual emission bands and SM spectra exhibited distribution with two emission maxima. These results indicate that two distinct environments exist in the thin PS film on a glass: a low polarity environment in the PS bulk and a high polarity environment near the PS/glass interface. It was also found that a PS film of <I>ca.</I> 10nm thick is sufficient to separate fluorophores from the charged species on glass surfaces.</P>

ATP Measurement Predicts Porcine Islet Transplantation Outcome in Nude Mice

Kim, Jae Hyeon,Park, Sang Gyu,Lee, Han Na,Lee, Yeon Yi,Park, Ho Sun,Kim, Hyong-Il,Yu, Jae Eun,Kim, Sun Hee,Park, Chung-Gyu,Ha, Jongwon,Kim, Sang Jun,Park, Kyong Soo Lippincott Williams Wilkins, Inc. 2009 Transplantation Vol.87 No.2

Current nude mice islet transplantation studies cannot be used prospectively. Therefore, to predict transplantation outcomes, reliable and rapid assays for islet quality assessment are warranted. This study evaluated the predictive power of the porcine islet ATP content on the outcomes of islet transplantation in nude mice. Here, we report that the ATP measurement using a small number of handpicked islets with a diameter of 100 to 150 μm is a good predictor of islet graft efficacy in nude mice. Using receiver-operator characteristic analysis, the area under the curve of the ATP content using a small number of handpicked islets was 0.867 (95% confidence interval 0.744–0.989, P<0.001). The sensitivity and the specificity measured were 83.3% and 73.3%, respectively. In conclusion, a simple and a rapid measurement of intraislet ATP content could be a promising substitute for current nude mice islet transplantation studies.

Evaluation of 3-hydroxy propionic acid production by Kluyveromyces marxianus through fluid flow analysis

Se hyong lee,Kyoung-Gon Kang,Doohyeon Kim,Deok-Ho Kwon,Woong Ki Jang,Byeon Hee Kim,Hojin Ha,Suk-Jin Ha 한국생산제조학회 2022 한국생산제조시스템학회 학술발표대회 논문집 Vol.2022 No.5

물오리나무 ( Alnus hirsuta ) 의 칼루스 유도 및 자엽유래 칼루스로부터의 원형질체 배양

김형하(Hyong Ha Kim),안정선(Chung Sun An) 한국식물학회 1990 Journal of Plant Biology Vol.33 No.4

Callus-inducing ability of Alnus hirsuta was examined by culturing various tissues (leaf, hypocotyl, cotyledon and seed) on NT (Nagata & Takebe) medium, supplemented with 2.5 μM 2,4-D. Leaf-orginated callus was cultured on media varying in auxin (IBA and NAA) and cytokinin (BAP) concentrations to examine the effects of auxin and cytokinin on callus growth. Maximum growth was obtained at 10μM IBA + 10μM BAP and 10μM NAA without cytokinin. Cell suspensions established from cotyledon-originated callus yielded viable protoplasts after incubation for 16-18 hours in an enzyme mixture (1% (w/v) Onozuka R-10, 0.5% (w/v) Macerozyme, CPW salts and 13% (w/v) mannitol, pH 5.8). Protoplasts were cultured on NT medium, supplemented with glucose, hormones and coconut milk. After 6 weeks of culture, protoplasts sustained cell divisions to form microcallus, which showed various colors from red to white.

베타3-아드레날린성 수용체 유전자 변이와 체지방 분포

윤태승,김용득,김혜순,김미정,서영성,권중혁,최진수,김정국,하승우,김보완,원규장,이형우,손호상,이지현,윤현대,김원호,윤영길,이인규 대한내분비학회 2003 Endocrinology and metabolism Vol.18 No.2

연구배경: 비만의 발생 원인은 크게 유전적인 요인과 환경적인 요인으로 나눌 수 있으며, 치근 비만과 관련된 유전자 연구가 활발히 진행되고 있다 지금 까지 알려진 비만 관련 유전자로는 Leptin, UCP, PPAR-r, β3-AR 유전자 등이 있으며, 이 중에서 β3-AR 유전자는 내장 지방에 주로 존재하면서 G 단백질과 결합하여, 아드레날린성 신호에 의해 c-AMP 증가로 지방 분해와 열 발생을 일으킨다. 이 유전자의 64번째 아미노산인 trpytophan이 arginine으로 치환된 변이에서 기초대사율 감소와 체중 증가가 있다는 보고가 있으나, 비만과 무관하다는 보고도 있어, 본 연구는 좀 더 많은 수를 대상으로 β3-AR 유전자 변이에 따른 비만과의 연관성을 확인 위해 시행되었다. 방법: 연구 대상자는 정상군 97명 비만군 101명으로 하여, β3-AR 유전자 변이에 따른 체질량지수, 허리둘레, 엉덩이둘레, 허리엉덩이둘레비, 복부 내장지방면적, 피하지방면적, 내장지방/피하지방면적비율을 비교하고, 총 콜레스테롤, 중성 지방, 고밀도 지단백, 저밀도 지단백 등을 비교하였다. 결과: β3-AR유전자의 다형성이 비만군에 있어 이형접합체변이 (Trp/Arg)와 동형접합체 변이 (Arg/Arg)비율이 43%, 5%로 정상인 36%, 1% 비해 높았으나 통계학적 유의성은 없었다. 복부 피하지방면적 비교에 있어 정상군(Trp/T게)에서는 213.9± 109.6 ㎠인 반면에 동형 접합체변이 (Trp/Arg)와 이형접합체변이 (Arg/Arg)군에서 각각, 244.0±127.7 ㎠, 323.9±189.8 ㎠로 통계학적 유의성이 있었으며, 그 외에 허리 둘레,엉덩이 둘레, 허리 엉덩이 둘레비, 내장지방면적, 내장지빈피하지방면적비 등은 유전자 변이군에서 전반적인 증가는 있었지만 통계학적 유의성은 없었다. 결론: 3-AR 유전자 변이와 비만과의 관련에 대한 본 연구에 의하면, β3-AR 유전자 변이에 따른 비만 연관성은 복부 피하지방면적 증가에 있어서 통계학적으로 유의하게 증가 소견 보였으며, 그 외 다른 비만 관련 인자들에 있어 통계학적 의의는 없으나 전반적인 증가 소견을 보여, 한국인에 있어 β3-AR 유전자 변이는 비만과 연관성이 있을 것이라는 결론을 얻을 수 있었다. Background: Reasons for obesity include environmental facotrs and, more largely so, genetic factors. There have been many studies on these genetic factors. So far, genes related to obesity such as Leptin, Uncoupling Protein(UCP), Peroxisome proliferator activated receptor-gamma (PPAR-γ), and Beta3-adrener-gic receptor(β3-AR) gene have been discovered. Among these, β3-AR is expressed in visceral adipose tissue and is thought to contribute to the regulation of resting metabolic rate and lipolysis. The missense mutation of β3-AR gene, resulting in replacement of tryptophan by arginine at position 64 (Trp64Arg), is associated with decreased resting metabolic rate and weight gain. We performed this study to determine if Trp64Arg polymorphism of β3-AR gene is associated with obesity in Koreans. Method: We investigated the relationship between the (33-AR gene mutation and body mass index (BMI), waist circumference, hip circumference, waist to hip ratio (WHR), area of subcutaneous fat, area of visceral fat, visceral to subcutaneous fat ratio (VSR), and lipid profile. 198 subjects were included in this study of which 97 were of normal weight and 101 were obese. Anthropometric data was obtained from physical examination and medical records. Result: In the cases of (33-AR gene mutation of the obese group, the ratio of Trp/Arg and Arg/Arg are 43% and 5%, respectively, which were higher than the normal group(36%, l%), although a statistical significant was not found. There was significant difference in the are of subcutaneous fat. Normal group (Trp/Trp) measured at 213.9k109.6 ㎠ versus 244.0k127.7 ㎠ (Trp/Arg) and 323.9k189.9 ㎠ (Arg/Arg) for the mutation groups. Circumference of waist, circumference of hip, WHR, area of visceral fat, and VSR were higher in the mutation groups than in normal subject, but not significantly different. Conclusion: These results suggest that a genetic mutation in the (33-AR gene can affect body fat composition, and is associated with obesity in Korean adults (J Kor SOC Endocrinol 18:184-192, 2003).

Dynamic genetic features of chromosomes revealed by comparison of soybean genetic and sequence-based physical maps

Lee, Woo Kyu,Kim, Namshin,Kim, Jiwoong,Moon, Jung-Kyung,Jeong, Namhee,Choi, Ik-Young,Kim, Sang Cheol,Chung, Won-Hyong,Kim, Hong Sig,Lee, Suk-Ha,Jeong, Soon-Chun Springer-Verlag 2013 TAG. Theoretical and applied genetics. Theoretisch Vol.126 No.4

Machine-Learning-Based Approach to Differential Diagnosis in Tuberculous and Viral Meningitis

Jeong Young-Seob,Jeon Minjun,Park Joung Ha,Kim Min-Chul,Lee Eunyoung,Park Se Yoon,Lee Yu-Mi,최승임,Park Seong Yeon,Park Ki-Ho,Kim Sung-Han,Jeon Min Huok,Choo Eun Ju,Kim Tae Hyong,Lee Mi Suk,Kim Tark 대한감염학회 2021 Infection and Chemotherapy Vol.53 No.1

Background: Tuberculous meningitis (TBM) is the most severe form of tuberculosis, but differentiating between the diagnosis of TBM and viral meningitis (VM) is difficult. Thus, we have developed machine-learning modules for differentiating TBM from VM. Material and Methods: For the training data, confirmed or probable TBM and confirmed VM cases were retrospectively collected from five teaching hospitals in Korea between January 2000 - July 2018. Various machine-learning algorithms were used for training. The machinelearning algorithms were tested by the leave-one-out cross-validation. Four residents and two infectious disease specialists were tested using the summarized medical information. Results: The training study comprised data from 60 patients with confirmed or probable TBM and 143 patients with confirmed VM. Older age, longer symptom duration before the visit, lower serum sodium, lower cerebrospinal fluid (CSF) glucose, higher CSF protein, and CSF adenosine deaminase were found in the TBM patients. Among the various machinelearning algorithms, the area under the curve (AUC) of the receiver operating characteristics of artificial neural network (ANN) with ImperativeImputer for matrix completion (0.85; 95% confidence interval 0.79 - 0.89) was found to be the highest. The AUC of the ANN model was statistically higher than those of all the residents (range 0.67 - 0.72, P <0.001) and an infectious disease specialist (AUC 0.76; P = 0.03). Conclusion: The machine-learning techniques may play a role in differentiating between TBM and VM. Specifically, the ANN model seems to have better diagnostic performance than the non-expert clinician.

Transmission Dose Estimation Algorithm for in vivo Dosimetry

Hyong Geur Yun,Eui Kyu Chie,Soon Nyung Huh,Hyoung Koo Lee,Hong Gyun Woo,Kyo Chul Shin,Siyong Kim,Sung Whan Ha 대한방사선방어학회 2002 방사선방어학회지 Vol.27 No.3

Administration of Goji (Lycium chinense Mill.) Extracts Improves Erectile Function in Old Aged Rat Model

Hyong Woo Moon,Jung Woo Park,Kyu Won Lee,Hyun Cheol Jeong,Jin Bong Choi,Sae Woong Choi,Woong Jin Bae,Hyuk Jin Cho,U-Syn Ha,Sung Hoo Hong,Jeong Ho Geum,Seong Bin Hong,Sae Woong Kim 대한남성과학회 2017 The World Journal of Men's Health Vol.35 No.1

Purpose: This study investigated the effect of goji (Lycium chinense Mill.) on erectile dysfunction in old-aged rats. Materials and Methods: Twenty-four 18-month-old male Sprague-Dawley rats (defined as old-aged rats) were used. Treatment groups contained eight rats each: a control group, goji extract of 150 mg/kg/day group, and goji extract of 300 mg/kg/day group. Treatment was by orogastric tube once daily for 6 weeks. After 6 weeks of treatment, testes weight, serum testosterone, superoxide dismutase, nitric oxide (NO)-cyclic guanosine monophosphate (cGMP)-related parameters, intracavernous pressure/mean arterial pressure, and histological changes were examined. Results: Treatments with goji extracts increased serum testosterone level, increased the expression of endothelial NO synthase, neuronal NO synthase, and cGMP, improved the oxidative stress marker, and decreased corporal fibrosis. Conclusions: Our results indicate that goji extract may have a positive effect on erectile dysfunction via its antioxidant effects.

중소규모 사업장의 환자의뢰체계 모형 개발

김정연,하은희,박혜숙,안형숙,안진숙,위자형 梨花女子大學校 醫科大學 醫科學硏究所 1999 EMJ (Ewha medical journal) Vol.22 No.2

연구 목적 : 본 연구에서는 중소규모사업장 근로자의 의료이용행태 및 의뢰체계에 대한 필요도를 조사하고, 이 자료를 근거로 이화여자대학교 의과대학 부속 목동병원과 동대문병원을 3차기관으로 하여 중소규모 사업장의 환자의뢰체계의 모형을 만들고자 하였다. 방법 : 1998년 4월부터 5월까지 대한산업보건협회 서울지부의 210개 보건관리대행 사업장 중 57개 사업장을 대상으로 각 사업장당 근로자 5명씩 무작위로 선발하여, 총 근로자 213명을 대상으로 설문조사를 하였다. 설문결과를 바탕으로 대한산업보건협회와 이화의료원의 환자 의뢰체계를 개발하였다.의뢰체계를 1년간 시범운영한 후 근로자들의 만족도를 조사하였다. 결과 : 의료기관 이용시의 불편사항으로는 접수, 예약, 절차 등의 절차가 번거로움과 근무시간과 진료시간의 불일치를 가장 많이 호소하였으며 생산직과 사무직의 차이는 없었다.외부 병원 연계의 필요성에 대하여는 젠체 대상자 중 76.7%가 필요하다고 대답한 반면 거리가 멀다면 68.8%가 이용하지 않겠다고 대답하였다. 의뢰체계가 도움을 주어야 할 내용으로는 가급적 빠른 진료, 질병이나 검사결과에 대한 자세한 설명, 진단과 치료의 신뢰감, 검사결과의 신속한 통보, 회송소견서 발부, 직업적 폭로요인 고려의 순으로 나타났다. 병원이용이 편리한 시간으로 일과 후(64.7%)가 가장 많이 나왔으며 85.6%에서 야간진료가 필요하다고 응답하였다. 조사대상자의 91.9%가 가족들이 진료의뢰체계에 포함되기를 원하였다. 98년 2월부터 99년 1월까지 의뢰체계를 통하여 이대병원을 이용한 총 근로자 수는 30명이었다. 근로자들의 병원서비스에 대한 만족도는 비교적 높게 나타났으나, 후송소견서가 교부, 직업성 요인의 고려는 잘 되지 않은 것으로 나타났다. 결론 : 앞으로 의뢰체계를 활성화하기 위해서는 근로자와 사업장에 대하여는 의뢰체계와 서비스 내용에 대한 정확한 홍보, 병원에서의 야간진료실시, 직업성 질환자의 진료를 위한 임상각과와 예방의학과의 공식적인 의뢰체계 수립이 필요하다고 생각된다. Objectives : This study was designed to develop the referral system of patient in small-sized industries and the medical delivery system in occupational health field. Methods : We sampled randomly 5 workers per each company in 57 companies and investigated the healthcare utilization behavior, the need for referral system. The survey was done from April 1. 1998 to May 30. 1998 and the respondents were 213. On the basis of the results, we planned model for the referral system between Korean Industrial Health Association(KIHA) and Ewha Medical Center(EMC). Results : The complaints of the utilization of medical service are cumbersome process of registration and reservation, and discord between hours of duty and consultation. The referral system is needed in 76.7% of total respondents. 85.6% of workers want the night clinic, and 91.3% want to be included their family in this referral system. We developed the referral system linking KIHA, EMC, and department of preventive medicine of Ewha Woman's University, which based on the result of survey. The service reflect the worker's the healthcare utilization behavior and need for referral system. The number of workers who referred by this system between Feb. 1998 and Jan. 1999 was 30, and replied by EMC after treating was 12. The number of referred patient is very law(30 spells), but the satisfaction of medical service is relatively high. Conclusion : To promote the referral system, it is needed to propaganda the service of referral system to workers and managers of each company, to improve of quality of services of the hospital, and to establish the official referral system between department of preventive medicine of Ewha woman's university and each clinical department of Ewha Medical Center.