MUC16 facilitates cervical cancer progression via JAK2/STAT3 phosphorylation‑mediated cyclooxygenase‑2 expression

Hui Shen,Meng Guo,Lu Wang,Xinyue Cui 한국유전학회 2020 Genes & Genomics Vol.42 No.2

Objectives MUC16 (mucin 16, also known as CA-125, cancer antigen 125, carcinoma antigen 125, or carbohydrate antigen 125) has been predicted as tumor biomarker for therapy. We determined to investigate effects and regulatory mechanism of MUC16 on cervical tumorigenesis. Methods Expression levels of MUC16 in cervical cancer cell lines was analyzed via qRT-PCR (quantitative real-time polymerase chain reaction). Knockdown of MUC16 was conducted via shRNA (Short hairpin RNA) transfection. MTT and colony formation assays were used to investigate effect of MUC16 on cell proliferation. Wound healing assay was utilized to detect migration and transwell assay to detect invasion. The underlying mechanism was demonstrated via western blot analysis. Results MUC16 was elevated in cervical cancer cell lines. MUC16 knockdown inhibited cell proliferation, invasion and migration. Gain- and loss-of functional assays revealed that over-expression of MUC16 activated Janus Kinase 2 (JAK2)/ signal transducer and activator of transcription 3 (STAT3) via phosphorylation, thus facilitating cyclooxygenase-2 (COX-2) expression, while knockdown of MUC16 demonstrated the reverse effect on JAK2/STAT3 activation and COX-2 expression. Moreover, inhibition of JAK2/STAT3 attenuated the regulation of MUC16 on COX-2. Conclusions MUC16 enhanced proliferation and invasion of cervical cancer cells via JAK2/STAT3 phosphorylation-mediated cyclooxygenase-2 expression, suggesting the potential therapeutic target ability of MUC16 to treat cervical cancer.

ปรากฏการณ์พหุนัยของคำว่า “กิน” ในภาษาไทย : การศึกษาตามแนวภาษาศาสตร์ปริชาน

( Lu Xin Hui ),( Park Kyung Eun ) 한국태국학회 2019 한국태국학회논총 Vol.25 No.2

This study aims to analyze the aspects of meaning extensions and conceptualization of the polysemous verb, “KIN (to eat)”, from a cognitive linguistic perspective. In this study, various concepts of cognitive linguistics are applied in the analysis, including prototype theory, image schema, and conceptual metaphors and windowing. The linguistic data used in the study is collected from the Thai National Corpus. The result of the study shows that the prototypical meaning, i.e. the most salient meaning, comes from the bodily experience of consuming food. The act of consumption is comprised of three steps: Step of getting or taking, Step of occupation, and Step of consumption & production of Energy, and each step become the basis of various meaning extensions. This study also analyzes negative implications of the Thai verb, KIN. The negative implications are found to be based on metaphorical concepts, EATING IS EGOISTIC ACTION and EATING IS GETTING OUT OF THE MIDDLE WAY. This study helps to understand the cognitive mechanism of polysemy and grammaticalization by presenting the motivation behind conceptualization of the prototypical meaning of the polysemous verb “KIN.” We hope for this study to contribute in both cognitive linguistics and teaching Thai as a foreign language.

Structure, chemical stability and electrical conductivity of perovskite La_0.6Sr_0.4M_0.3Fe_0.7O_{3−<i>δ</i>} (M=Co, Ti) oxides

Lu, Hui,Kim, Jong Pyo,Son, Sou Hwan,Park, Jung Hoon Elsevier 2010 Materials science and engineering B. Advanced Func Vol.166 No.2

<P><B>Abstract</B></P><P>The perovskite La<SUB>0.6</SUB>Sr<SUB>0.4</SUB>M<SUB>0.3</SUB>Fe<SUB>0.7</SUB>O<SUB>3−<I>δ</I></SUB> (M=Co, Ti) powders have been synthesized by the citrate gel method. The structural and chemical stability of the La<SUB>0.6</SUB>Sr<SUB>0.4</SUB>M<SUB>0.3</SUB>Fe<SUB>0.7</SUB>O<SUB>3−<I>δ</I></SUB> (M=Co, Ti) oxides were studied by X-ray diffraction (XRD), differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA) techniques. The electrical conductivities of the sintered La<SUB>0.6</SUB>Sr<SUB>0.4</SUB>M<SUB>0.3</SUB>Fe<SUB>0.7</SUB>O<SUB>3−<I>δ</I></SUB> (M=Co, Ti) ceramics were measured. The results demonstrate the chemical stability in H<SUB>2</SUB>/helium (He) atmosphere of the La<SUB>0.6</SUB>Sr<SUB>0.4</SUB>Ti<SUB>0.3</SUB>Fe<SUB>0.7</SUB>O<SUB>3−<I>δ</I></SUB> oxide is improved significantly compared to that of the La<SUB>0.6</SUB>Sr<SUB>0.4</SUB>Co<SUB>0.3</SUB>Fe<SUB>0.7</SUB>O<SUB>3−<I>δ</I></SUB> oxide. The incorporation of Ti<SUP>3+/4+</SUP> ions in the perovskite structure can significantly stabilize the neighboring oxygen octahedral due to the stronger bonding strength, leading to the enhanced structural and chemical stability of the La<SUB>0.6</SUB>Sr<SUB>0.4</SUB>Ti<SUB>0.3</SUB>Fe<SUB>0.7</SUB>O<SUB>3−<I>δ</I></SUB>. In addition, the perovskite La<SUB>0.6</SUB>Sr<SUB>0.4</SUB>M<SUB>0.3</SUB>Fe<SUB>0.7</SUB>O<SUB>3−<I>δ</I></SUB> (M=Co, Ti) oxides possess much higher chemical stability in CO<SUB>2</SUB>/He atmosphere than that of Ba<SUB>0.5</SUB>Sr<SUB>0.5</SUB>Co<SUB>0.8</SUB>Fe<SUB>0.2</SUB>O<SUB>3−<I>δ</I></SUB> oxide, in which the perovskite structure is destroyed completely in a flowing CO<SUB>2</SUB>-containing atmosphere.</P>

El estudio de la coligacin basado en el corpus

Hui-Chan Lu 한국스페인어문학회 2007 스페인어문학 Vol.0 No.45

El presente trabajo se dedica a estudiar la coligación, es decir, la asociación entre el léxico y la sintaxis, basándonos en el análisis de dos tipos de corpus, por un lado el de los aprendices taiwaneses, que representa la lengua adquirida, extrayendo datos del CAET (Corpus de Aprendices de Español Taiwaneses); y por otro, el de los hablantes nativos de español, chino e inglés, que representan la lengua natural, analizando los datos de tres corpus, el Corpus del Español, el Sinica Corpus y el British National Corpus). Nuestro objetivo será asimilar equitativamente de las dos variedades de lengua y ayudar a los aprendices taiwaneses que aprenden español como segunda lengua extranjera a aumentar su nivel léxico con eficacia. La contribución principal del presente estudio estriba en el hecho de relacionar cuatro corpus de diferentes aspectos para dar luz acerca de diversas cuestiones: el aprendizaje léxicogramatical, la identificación de la relación entre la lengua aprendida y la natural española, y el examen de diferentes factores que pueden intervenir en la adquisición de una segunda lengua extranjera.

Biological characterization of mesenchymal stem cells from bovine umbilical cord

Hui Xiong,Wei Jun Guan,Yue Hui Ma,Chunyu Bai,Shuang Wu,Yuhua Gao,Taofeng Lu,Qingyun Hu 한국통합생물학회 2014 Animal cells and systems Vol.18 No.1

Mesenchymal stem cells (MSCs) are multi-potential cells that are able to proliferate and differentiate into othercell types. Much research has been done on the MSCs from the umbilical cord (UCMSCs) in human, mice, andavian, but little literature has been published about these cells in big livestock. Here, we choose Luxi cattle asthe experimental animal, we describe an external culture of the UCMSCs from it and summarize the biologicalcharacteristics of these cells, e.g., morphologic appearance, surface antigens, colony-forming ability, geneexpression, and differentiation potential were detected via using immunofluorescence and reverse transcriptionpolymerase chain reaction (RT-PCR). The induced cells, osteoblast, lipoblast, hepatocyte, islet cells, andneurocyte were identified by Alizarin red staining, Oil-red-O staining, Periodic acid-schiff staining, andDithizone staining and RT-PCR detection for specific genes. Results suggest that biological characteristics ofthe UCMSCs were similar to those of MSCs previously analyzed. The primary UCMSCs were sub-cultured topassage 32, the UCMSCs expressed gene CD29, CD44, CD73, CD90, and CD166, induced cells illustratedtypical staining, and expressed specific genes, which indicate that the UCMSCs could be a novel alternativesource of MSCs for experimental and clinical applications.

A Corpus-Based Study on Language Features and Literary Themes in the Yellow Wall-Paper and Herland by Charlotte Perkins Gilman

Lu, Hui-Chuan,Liu, Kai-Ling,Yeh, Chien-Ting,Chen, Ya-Jie Institute for Corpus Research 2022 Asia pacific journal of corpus research Vol.3 No.1

This study aims to apply corpus-based approach to analyze The Yellow Wall-Paper and Herland written by Charlotte Perkins Gilman, a women's rights activist in the late nineteenth-century America. Although both works have attracted feminists' attention to the woman question that concerned Gilman, discussion on her language features and their relation to the literary themes of these two works is still in need. In this corpus-based analysis, we argue that the main themes of different literary works can be revealed through linguistic patterns identified by number and gender features of nouns and pronouns in the contrast of two works and a balanced corpus. The linguistic features (number and gender) have been related with two themes, the 'group and individual' and the 'feminine and masculine', and are further interpreted in terms of mothering and feminine consciousness. By adopting linguistic approach, our study provides quantitative and qualitative evidence to verify the established themes and arguments of these literary texts.

The Logical Starting Point and Practical Pursuit of Ideological and Political Education in Physical Education from the Perspective of Fostering Virtue through Education

Hui Zhang,Haoyu Lu,Shuliang Xu,Baichao Xu,Lu Zongxia 아시아건강운동학회 2022 Journal of Asian Society for Health & Exercise Vol.4 No.2

Roseovarius tibetensis sp. nov., a halophilic bacterium isolated from Lake LongmuCo on Tibetan Plateau

Hui-bin Lu,Xiao-feng Xue,Dorji Phurbu,Peng Xing,Qing-long Wu 한국미생물학회 2018 The journal of microbiology Vol.56 No.11

Two Gram-stain negative halophilic strains, designated as LM2T and LM4, were isolated from Lake LongmuCo on Tibetan Plateau. These two strains were aerobic, catalaseand oxidase- positive, nonmotile and rod-shaped organisms. Phylogenetic analysis based on 16S rRNA gene sequences indicated that LM2T and LM4 belong to the genus Roseovarius, with Roseovarius tolerans EL-172T (97.3% and 97.4% 16S rRNA gene sequence similarity, respectively) and Roseovarius azorensis SSW084T (95.5% and 95.6% 16S rRNA gene sequence similarity, respectively) as their closest neighbors. Q-10 was the sole respiratory quinone of these two strains. The major fatty acids were C18:1 ω7c/C18:1 ω6c, C16:0, C19:0 cyclo ω8c, and 11-methyl C18:1 ω7c. The polar lipids included phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phospholipid of unknown structure containing glucosamine, and unidentified aminolipid. The DNA G + C content was between 64.2 and 64.5 mol%. DNA-DNA hybridization showed 96.7% relatedness between LM2T and LM4, 24.9% relatedness between LM2T and R. tolerans EL-172T, and 36.3% relatedness between LM4 and R. tolerans EL-172T. Based on phylogenetic analysis, DNA-DNA hybridization, a range of physiological and biochemical characteristics, LM2T and LM4 belong to the same species and were clearly distinguished from the type strains of the genus Roseovarius. It was evident that LM2T and LM4 could be classified as a novel species of the genus Roseovarius, for which the name Roseovarius tibetensis sp. nov. is proposed. The type strain is LM2T (= CGMCC 1.16230T = KCTC 62028T).

A taxonomic study of genus Teleogryllus from East Asia (Insecta: Orthoptera: Gryllidae)

Hui Lu,Xiao-Yin Wang,Han-Qiang Wang,Kai Li,Zhu-Qing He 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.2

Crickets belonging to Teleogryllus spp. are widely distributed in East Asia and they are difficult to distinguish. In this study, a phylogenetic tree of Teleogryllus is reconstructed by COI gene, and all species can be distinguished well. The possible evolutionary histories of male genitalia, songs and life history are discussed. The result supports establishing subgenera Macroteleogryllus and Brachyteleogryllus of Teleogryllus spp. from East Asia. T. boninensis is moved to Brachyteleogryllus. The calling song of four species includes both long and short chirps, and the rest just have single chirps. The species inhabit sympatrically trend to have different type of calling song. Egg overwinter type evolved several times independently.

Preliminary study on the effect of using unmanned aerial vehicles (UAVs) to control Spodoptera frugiperda

Lu Hui,Li Fen,Zhu Xiaoming,Tang Jihong,Lyu Baoqian,Wu Shaoying 한국곤충학회 2021 Entomological Research Vol.51 No.9

Spraying insecticides by unmanned aerial vehicle (UAV) is an effective method to prevent and control Spodoptera frugiperda. This study investigated the influences of different UAV types, spray adjuvants, spray times, maize growth periods, and insecticides on S. frugiperda in three cities, Hainan Province, China. The results showed that at least two times are needed to spray insecticides using UAVs when controlling the S. frugiperda. Both UAVs had a substantially higher control effect and lower cost than artificial spray methods. Oil spray adjuvant Refei was more suitable for both UAVs than Tiancheng to control pests. Chlorantraniliprole had the best control effect of up to 90%. The control effects at the seedling stage and small bell stage of S. frugiperda were 94.3% and 91.3%, respectively. Spraying at 7:00 am and 7:00 pm by UAVof T20 had good control effect on S. frugiperda in three cities.