MUC16 facilitates cervical cancer progression via JAK2/STAT3 phosphorylation‑mediated cyclooxygenase‑2 expression

Hui Shen,Meng Guo,Lu Wang,Xinyue Cui 한국유전학회 2020 Genes & Genomics Vol.42 No.2

Objectives MUC16 (mucin 16, also known as CA-125, cancer antigen 125, carcinoma antigen 125, or carbohydrate antigen 125) has been predicted as tumor biomarker for therapy. We determined to investigate effects and regulatory mechanism of MUC16 on cervical tumorigenesis. Methods Expression levels of MUC16 in cervical cancer cell lines was analyzed via qRT-PCR (quantitative real-time polymerase chain reaction). Knockdown of MUC16 was conducted via shRNA (Short hairpin RNA) transfection. MTT and colony formation assays were used to investigate effect of MUC16 on cell proliferation. Wound healing assay was utilized to detect migration and transwell assay to detect invasion. The underlying mechanism was demonstrated via western blot analysis. Results MUC16 was elevated in cervical cancer cell lines. MUC16 knockdown inhibited cell proliferation, invasion and migration. Gain- and loss-of functional assays revealed that over-expression of MUC16 activated Janus Kinase 2 (JAK2)/ signal transducer and activator of transcription 3 (STAT3) via phosphorylation, thus facilitating cyclooxygenase-2 (COX-2) expression, while knockdown of MUC16 demonstrated the reverse effect on JAK2/STAT3 activation and COX-2 expression. Moreover, inhibition of JAK2/STAT3 attenuated the regulation of MUC16 on COX-2. Conclusions MUC16 enhanced proliferation and invasion of cervical cancer cells via JAK2/STAT3 phosphorylation-mediated cyclooxygenase-2 expression, suggesting the potential therapeutic target ability of MUC16 to treat cervical cancer.

A Review on Microbial Decaffeination

Hui-Shi Wu,Xin-Qiang Zheng,Yue-Rong Liang,Jian-Hui Ye,Jian-Liang Lu 한국차학회 2015 한국차학회지 Vol.- No.S

Exclusive exposure of caffeine might bring some adverse effects to human health, and cause some risks to environment. Microbial decaffeination might be a new alternative approach because of its high specificity and efficiency. Many studies revealed that several microorganisms can degrade caffeine, including genera Alcaligenes,Rhodococcus, Klebsiella, Acinetobacter, Enterobacter, Stenotrophomonas and Pseudomonas. Biodegradation of caffeine can be significantly influenced by temperature, pH, aeration rate and initial caffeine concentration of the incubation. There are two main pathways of caffeine biodegradation: C-8 oxidation and N-demethylation, while N-demethylation is the general catabolism pathway in bacteria. Application of microbial decaffeination has also been discussed in this review.

Attenuation of Experimental Autoimmune Hepatitis in Mice with Bone Mesenchymal Stem Cell-Derived Exosomes Carrying MicroRNA-223-3p

Lu, Feng-Bin,Chen, Da-Zhi,Chen, Lu,Hu, En-De,Wu, Jin-Lu,Li, Hui,Gong, Yue-Wen,Lin, Zhuo,Wang, Xiao-Dong,Li, Ji,Jin, Xiao-Ya,Xu, Lan-Man,Chen, Yong-Ping Korean Society for Molecular and Cellular Biology 2019 Molecules and cells Vol.42 No.12

MicroRNA-223-3p (miR-223-3p) is one of the potential microRNAs that have been shown to alleviate inflammatory responses in pre-clinical investigations and is highly encased in exosomes derived from bone mesenchymal stem cells (MSC-exosomes). MSC-exosomes are able to function as carriers to deliver microRNAs into cells. Autoimmune hepatitis is one of the challenging liver diseases with no effective treatment other than steroid hormones. Here, we examined whether MSC-exosomes can transfer miR-223-3p to treat autoimmune hepatitis in an experimental model. We found that MSC-exosomes were successfully incorporated with miR-223-3p and delivered miR-223-3p into macrophages. Moreover, there was no toxic effect of exosomes on the macrophages. Furthermore, treatments of either exosomes or exosomes with miR-223-3p successfully attenuated inflammatory responses in the liver of autoimmune hepatitis and inflammatory cytokine release in both the liver and macrophages. The mechanism may be related to the regulation of miR-223-3p level and STAT3 expression in the liver and macrophages. These results suggest that MSC-exosomes can be used to deliver miR-223-3p for the treatment of autoimmune hepatitis.

Pathogenesis Division of Black Rot Bacteria of Chinese Cabbage in Shaanxi Central Productive Area

Lu-gang Zhang,Yan Lu,Mai-xia Hui,Ming-ke Zhang 한국원예학회 2008 한국원예학회 기타간행물 Vol.- No.-

Complex dental anomalies in a belatedly diagnosed cleidocranial dysplasia patient

Hui Lu,Binghui Zeng,Dongsheng Yu,Xiangyi Jing,Bin Hu,Wei Zhao,Yiming Wang 대한영상치의학회 2015 Imaging Science in Dentistry Vol.45 No.3

Cleidocranial dysplasia (CCD) is a rare congenital disorder, typically characterized by persistently open skull sutures, aplastic or hypoplastic clavicles, and supernumerary teeth. Mutations in the gene encoding the runt-related transcription factor 2 (RUNX2) protein are responsible for approximately two thirds of CCD patients. We report a 20-year-old CCD patient presenting not only with typical skeletal changes, but also complex dental anomalies. A previously undiagnosed odontoma, 14 supernumerary teeth, a cystic lesion, and previously unreported fused primary teeth were discovered on cone-beam computed tomography (CBCT) scans. Mutation analysis identified the causal c.578G>A (p.R193Q) mutation in the RUNX2 gene. At 20 years of age, the patient had already missed the optimal period for dental intervention. This report describes the complex dental anomalies in a belatedly diagnosed CCD patient, and emphasizes the significance of CBCT assessment for the detection of dental anomalies and the importance of early treatment to achieve good outcomes.

The Acquisition of Spanish Clitic Pronouns as a Third Language: A Corpus-based Study

Lu, Hui-Chuan,Cheng, An Chung,Chu, Yu-Hsin Institute for Corpus Research 2020 Asia pacific journal of corpus research Vol.1 No.2

This corpus-based study investigated third language acquisition by Taiwanese college students in learning Spanish clitic pronouns at beginning and intermediate levels. It examined the acquisition sequences of Spanish clitic pronouns of the Chinese-speaking learners whose second language was English and third language was Spanish. The results indicated that indirect object pronouns (OP) preceded direct OP (case), first person preceded third person OP (person), masculine preceded feminine OP (gender), and animate preceded inanimate OP (animacy). The findings presented similar patterns as those of previous studies on English-speaking learners of Spanish. In further comparisons of the target forms in Chinese, English, and Spanish, the results suggested that L1 Chinese had strong influence on L3 Spanish, which accounts for the challenges that Taiwanese learners of Spanish face as they learn the Spanish clitic pronouns in the beginning stage.

ปรากฏการณ์พหุนัยของคำว่า “กิน” ในภาษาไทย : การศึกษาตามแนวภาษาศาสตร์ปริชาน

( Lu Xin Hui ),( Park Kyung Eun ) 한국태국학회 2019 한국태국학회논총 Vol.25 No.2

This study aims to analyze the aspects of meaning extensions and conceptualization of the polysemous verb, “KIN (to eat)”, from a cognitive linguistic perspective. In this study, various concepts of cognitive linguistics are applied in the analysis, including prototype theory, image schema, and conceptual metaphors and windowing. The linguistic data used in the study is collected from the Thai National Corpus. The result of the study shows that the prototypical meaning, i.e. the most salient meaning, comes from the bodily experience of consuming food. The act of consumption is comprised of three steps: Step of getting or taking, Step of occupation, and Step of consumption & production of Energy, and each step become the basis of various meaning extensions. This study also analyzes negative implications of the Thai verb, KIN. The negative implications are found to be based on metaphorical concepts, EATING IS EGOISTIC ACTION and EATING IS GETTING OUT OF THE MIDDLE WAY. This study helps to understand the cognitive mechanism of polysemy and grammaticalization by presenting the motivation behind conceptualization of the prototypical meaning of the polysemous verb “KIN.” We hope for this study to contribute in both cognitive linguistics and teaching Thai as a foreign language.

Halomonas tibetensis sp. nov., isolated from saline lakes on Tibetan Plateau

Hui-bin Lu,Peng Xing,Lei Zhai,Dorji Phurbu,Qian Tang,Qing-long Wu 한국미생물학회 2018 The journal of microbiology Vol.56 No.7

Strains pyc13T and ZGT13 were isolated from Lake Pengyan and Lake Zigetang on Tibetan Plateau, respectively. Both strains were Gram-negative, catalase- and oxidase-positive, aerobic, rod-shaped, nonmotile, and nonflagellated bacteria. Phylogenetic analysis based on 16S rRNA gene sequences showed that strains pyc13T and ZGT13 belong to the genus Halomonas, with Halomonas alkalicola 56-L4-10aEnT as their closest neighbor, showing 97.4% 16S rRNA gene sequence similarity. The predominant respiratory quinone of both strains was Q-9, with Q-8 as a minor component. The major fatty acids of both strains were C18:1 ω6c/C18:1 ω7c, C16:1 ω6c/C16:1 ω7c, C16:0, and C12:0 3OH. The polar lipids of both strains consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, glycolipid, phospholipids of unknown structure containing glucosamine, and unidentified phospholipids. The DNA G + C content of pyc13T and ZGT13 were 62.6 and 63.4 mol%, respectively. The DNA-DNA hybridization values of strain pyc13T were 34, 41, 61, 35, and 35% with the reference strains H. alkalicola 56-L4-10aEnT, H. sediminicola CPS11T, H. mongoliensis Z-7009T, H. ventosae Al12T, and H. fontilapidosi 5CRT, respectively. Phenotypic, biochemical, genotypic, and DNA-DNA hybridization data showed that strains pyc13T and ZGT13 represent a new species within the genus Halomonas, for which the name H. tibetensis sp. nov. is proposed. The type strain is pyc13T (= CGMCC 1.15949T = KCTC 52660T).

ปรากฏการณ์พหุนัยของคำว่า “กิน” ในภาษาไทย : การศึกษาตามแนวภาษาศาสตร์ปริชาน

Lu Xin Hui,박경은 한국태국학회 2019 한국태국학회논총 Vol.25 No.2

This study aims to analyze the aspects of meaning extensions and conceptualization of the polysemous verb, “KIN (to eat)”, from a cognitive linguistic perspective. In this study, various concepts of cognitive linguistics are applied in the analysis, including prototype theory, image schema, and conceptual metaphors and windowing. The linguistic data used in the study is collected from the Thai National Corpus. The result of the study shows that the prototypical meaning, i.e. the most salient meaning, comes from the bodily experience of consuming food. The act of consumption is comprised of three steps: Step of getting or taking, Step of occupation, and Step of consumption & production of Energy, and each step become the basis of various meaning extensions. This study also analyzes negative implications of the Thai verb, KIN. The negative implications are found to be based on metaphorical concepts, EATING IS EGOISTIC ACTION and EATING IS GETTING OUT OF THE MIDDLE WAY. This study helps to understand the cognitive mechanism of polysemy and grammaticalization by presenting the motivation behind conceptualization of the prototypical meaning of the polysemous verb “KIN.” We hope for this study to contribute in both cognitive linguistics and teaching Thai as a foreign language.

Estimation of load and resistance factors based on the fourth moment method

Lu, Zhao-Hui,Zhao, Yan-Gang,Ang, Alfredo H.S. Techno-Press 2010 Structural Engineering and Mechanics, An Int'l Jou Vol.36 No.1

The load and resistance factors are generally obtained using the First Order Reliability Method (FORM), in which the design point should be determined and derivative-based iterations have to be used. In this paper, a simple method for estimating the load and resistance factors using the first four moments of the basic random variables is proposed and a simple formula for the target mean resistance is also proposed to avoid iteration computation. Unlike the currently used method, the load and resistance factors can be determined using the proposed method even when the probability density functions (PDFs) of the basic random variables are not available. Moreover, the proposed method does not need either the iterative computation of derivatives or any design points. Thus, the present method provides a more convenient and effective way to estimate the load and resistance factors in practical engineering. Numerical examples are presented to demonstrate the advantages of the proposed fourth moment method for determining the load and resistance factors.