http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
( Yue Qi ),( Yu-Xiang Huang ),( Hong-Xia Ma ),( Wen-Ji Yu ),( Nam-Hun Kim ),( Ya-Hui Zhang ) 한국목재공학회 2019 목재공학 Vol.47 No.3
Effects of a novel mold inhibitor specifically for bamboo, on the properties of composite products have been confirmed in this study. The mechanical and dimensional stability properties of bamboo fiber-based composites (BFBCs) from different bamboo species were also investigated. The results showed that Burmanica Gamble possessed the highest values of modulus of elasticity (MOE) of 33.2 GPa, modulus of rupture (MOR) of 286.9 MPa, compressive strength of 182.6 MPa and shear strength of 24.0 MPa. By contrast, Phyllostochys heterocycla among all of species showed the lowest MOE of 16.3 GPa, MOR of 170.3 MPa and compressive strength of 128.9 MPa were the lowest among all of species. Moreover, there is a remarkable variation in the swelling and water absorption between the samples with 4 h and 28 h water immersion treatment, especially Phyllostachys iridenscens. Overall, the results suggested that TCIT (Tebuconazole and 3(2H)-isothiazolone) had no significant effect on the mechanical properties compared with the control condition, and it would be utilized as an antimould of BFBCs manufacturing.
Hui Zhang,Junxi Zheng,Li Yi,Yue Li,Zhe Ma,Hongjie Fan,Chengping Lu 한국미생물학회 2014 The journal of microbiology Vol.52 No.11
Streptococcus suis, a major swine pathogen, is an emergingzoonotic agent that causes meningitis and septic shock. Bacterialcell wall and secreted proteins are often involved ininteractions with extracellular matrix proteins (ECMs), whichplay important roles in the initial steps of pathogenesis. Inthis study, 2D SDS-PAGE, western blotting-based bindingaffinity measurements, and microtiter plate binding assayswere used to identify cell wall and secreted proteins from S. suis that interact with fibronectin and collagen type І. Weidentified six proteins from S. suis, including three proteins(translation elongation factor G, oligopeptide-binding proteinOppA precursor, and phosphoglycerate mutase) thatshow both fibronectin and collagen type І binding activity. To the best of our knowledge, these three newly identifiedproteins had no previously reported fibronectin or collagentype І binding activity. Overall, the aim in this study was toidentify proteins with ECM binding activity from S. suis andit represents the first report of six new proteins from S. suisthat interact with fibronectin or collagen type І.
Biological characterization of mesenchymal stem cells from bovine umbilical cord
Hui Xiong,Wei Jun Guan,Yue Hui Ma,Chunyu Bai,Shuang Wu,Yuhua Gao,Taofeng Lu,Qingyun Hu 한국통합생물학회 2014 Animal cells and systems Vol.18 No.1
Mesenchymal stem cells (MSCs) are multi-potential cells that are able to proliferate and differentiate into othercell types. Much research has been done on the MSCs from the umbilical cord (UCMSCs) in human, mice, andavian, but little literature has been published about these cells in big livestock. Here, we choose Luxi cattle asthe experimental animal, we describe an external culture of the UCMSCs from it and summarize the biologicalcharacteristics of these cells, e.g., morphologic appearance, surface antigens, colony-forming ability, geneexpression, and differentiation potential were detected via using immunofluorescence and reverse transcriptionpolymerase chain reaction (RT-PCR). The induced cells, osteoblast, lipoblast, hepatocyte, islet cells, andneurocyte were identified by Alizarin red staining, Oil-red-O staining, Periodic acid-schiff staining, andDithizone staining and RT-PCR detection for specific genes. Results suggest that biological characteristics ofthe UCMSCs were similar to those of MSCs previously analyzed. The primary UCMSCs were sub-cultured topassage 32, the UCMSCs expressed gene CD29, CD44, CD73, CD90, and CD166, induced cells illustratedtypical staining, and expressed specific genes, which indicate that the UCMSCs could be a novel alternativesource of MSCs for experimental and clinical applications.
Ma, Li,Du, Lei,Chen, Hui,Sun, Yue,Huang, Shan,Zheng, Xianliang,Kim, Eung-Soo,Li, Shengying,Elliot, M. A. American Society for Microbiology 2015 Applied and environmental microbiology Vol.81 No.18
<B>ABSTRACT</B><P>The cytochrome P450 enzyme CYP-sb21 fromSebekia benihanais capable of catalyzing the site-specific hydroxylation of the immunosuppressant cyclosporine (CsA), leading to the single product γ-hydroxy-<I>N</I>-methyl-l-Leu4-CsA (CsA-4-OH). Unlike authentic CsA, this hydroxylated CsA shows significantly reduced immunosuppressive activity while it retains a side effect of CsA, the hair growth stimulation effect. Although CYP-sb21 was previously identified to be responsible for CsA-specific hydroxylation<I>in vivo</I>, the<I>in vitro</I>activity of CYP-sb21 has yet to be established for a deeper understanding of this P450 enzyme and further reaction optimization. In this study, we reconstituted the<I>in vitro</I>activity of CYP-sb21 by using surrogate redox partner proteins of bacterial and cyanobacterial origins. The highest CsA site-specific hydroxylation activity by CYP-sb21 was observed when it was partnered with the cyanobacterial redox system composed of<I>se</I>Fdx and<I>se</I>FdR fromSynechococcus elongatusPCC 7942. The best bioconversion yields were obtained in the presence of 10% methanol as a cosolvent and an NADPH regeneration system. A heterologous whole-cell biocatalyst usingEscherichia coliwas also constructed, and the permeability problem was solved by using<I>N</I>-cetyl-<I>N</I>,<I>N</I>,<I>N</I>-trimethylammonium bromide (CTAB). This work provides a useful example for reconstituting a hybrid P450 system and developing it into a promising biocatalyst for industrial application.</P>
Research on the Cutting Performances and Wear Mechanisms of TiAlCrN Coated Tools during Dry Turning
Hui-Bo He,Hua-Ying Li,Xian-Ying Zhang,Qi-Bin Yue,Jun Zhang,Lu Ma,Yuan-ming Li 한국정밀공학회 2019 International Journal of Precision Engineering and Vol.20 No.2
Physical vapour deposition technique was used to deposit TiAlCrN coatings on the YT15 tungsten carbide inserts. The dry turning tests of 20CrMo steel were carried out to evaluate performances of TiAlCrN coated and uncoated tools on the CA6140A lathe. The effect of the two kinds of tools on cutting forces, cutting temperature, surface roughness and tool wear had been investigated to assess the performance of TiAlCrN coated tools. The results showed that the cutting force and cutting temperature obtained by TiAlCrN coated tools were decreased and the TiACrN coated tools produced a better surface finish in comparison with the uncoated tools. The TiAlCrN coated tools yield working life about 45 min, which was two times of that for uncoated tools. The wear mechanisms of the TiAlCrN coated tools were mainly oxidation and boundary wear, accompanied with diffusion wear.
( Ya-hui Zhang ),( Yu-xiang Huang ),( Hong-xia Ma ),( Wen-ji Yu ),( Yue Qi ) 한국목재공학회 2018 목재공학 Vol.46 No.4
In this study, the dimensional stability and mechanical properties of bamboo fiber-based composites (BFBCs) were studied at two pressing manufacturing processes, i.e., hot- and cold- pressing, and were compared with three density parameters (1.0, 1.1, and 1.2 kg/㎥). Width swelling ratio (WSR), thickness swelling ratio (TSR), and water absorption ratio (WAR) were calculated for water immersions of 4 and 28 h. WSR, TSR, and WAR for specimens immersed for 28 h were higher than those for 4 h treatment, which shows that the immersion time has a significant influence on the dimensional stabilities of BFBCs. Moreover, the positive linear relations between density and dimensional were observed at both the pressing ways, indicating that the WSR, TSR, and WAR decreased with an increase in the density of BFBCs. The compressive strength, shear strength, modulus of rupture (MOR), and modulus of elasticity (MOE) were determined. The compressive strength, MOR, and MOE of hot-pressed specimens were significantly higher than those for the cold-pressed specimens, which are also directly proportional to density. Moreover, the samples with the highest density of 1.2 kg/㎥ performed high values on mechanical properties in both the manufacturing methods.
( Yi-xiao Ma ),( Xiao-han Wu ),( Hui-shi Wu ),( Zhan-bo Dong ),( Jian-hui Ye ),( Xin-qiang Zheng ),( Yue-rong Liang ),( Jian-liang Lu ) 한국미생물생명공학회(구 한국산업미생물학회) 2018 Journal of microbiology and biotechnology Vol.28 No.7
The degradation efficiency and catabolism pathways of the different methylxanthines (MXs) in isolated caffeine-tolerant strain Pseudomonas putida CT25 were comprehensively studied. The results showed that the degradation efficiency of various MXs varied with the number and position of the methyl groups on the molecule (i.e., xanthine > 7-methylxanthine ≈ theobromine > caffeine > theophylline > 1-methylxanthine). Multiple MX catabolism pathways coexisted in strain CT25, and a different pathway would be triggered by various MXs. Demethylation dominated in the degradation of N-7-methylated MXs (such as 7- methylxanthine, theobromine, and caffeine), where C-8 oxidation was the major pathway in the catabolism of 1-methylxanthine, whereas demethylation and C-8 oxidation are likely both involved in the degradation of theophylline. Enzymes responsible for MX degradation were located inside the cell. Both cell culture and cell-free enzyme assays revealed that N-1 demethylation might be a rate-limiting step for the catabolism of the MXs. Surprisingly, accumulation of uric acid was observed in a cell-free reaction system, which might be attributed to the lack of activity of uricase, a cytochrome c-coupled membrane integral enzyme.
Transmembrane Protein 166 Expression in Esophageal Squamous Cell Carcinoma in Xinjiang, China
Sun, Wei,Ma, Xiu-Min,Bai, Jing-Ping,Zhang, Guo-Qing,Zhu, Yue-Jie,Ma, Hai-Mei,Guo, Hui,Chen, Ying-Yu,Ding, Jian-Bing Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.8
Objective: Transmembrane protein 166 (TMEM166) expression in esophageal squamous cell carcinoma (ESCC) and remote normal esophageal tissues was examined to assess any role in tumour biology. Methods: TMEM166 mRNA expression in 36 cases with ESCC (36 tumour samples, 36 remote normal esophageal tissue samples) was detected by RT-PCR. TMEM166 protein expression was analysed in paraffin-embedded tissue samples from the same cases by immunohistochemistry. Results: Semi-quantitative analysis showed TMEM166 mRNA expression in ESCCs to be significantly lower than in remote normal esophageal tissues ($0.759{\pm}0.713$ vs. $2.622{\pm}1.690$, P=0.014). TMEM166 protein expression was also significantly reduced (69.4% vs. 94.4%, P<0.01). Conclusion: TMEM166 mRNA and protein expression demonstrated significant reduction in ESCCs compared with remote esophageal tissues, albeit with no correlation with tumour size, differentiation, stage, and lymph node metastasis, suggesting a role in regulating autophagic and apoptotic processes in the ESCC.
Research on Friction Characteristics of AlCrN and TiAlSiN Coatings and Properties of Coated Tools
Qi-Bin Yue,Hui-Bo He,Hua-Ying Li,Jun Zhang,Yuan-ming Li,Lu Ma 한국정밀공학회 2019 International Journal of Precision Engineering and Vol.20 No.9
AlCrN and TiAlSiN coatings were deposited on the surface of 30CrMnSi steel and cemented carbide YT15 by multi-arc ion plating technique. A scanning electron microscopy was used to observe the surface morphologies of both coatings. The reciprocating sliding tests of AlCrN and TiAlSiN coating were performed to investigate the friction coefficients and the wear mechanisms of both coatings were analyzed as well. Dry machining tests on 30CrMnSi hardened steel were carried out with the AlCrN and TiAlSiN coated tools on a CA6140A lathe. The effects of cutting speed on cutting forces and cutting temperatures of AlCrN and TiAlSiN coated tools were obtained and analyzed. The microcosmic micrographs of wear areas of both coated tools were observed and investigated by scanning electron microscope and energy dispersive spectrum. The results show that the hardness and bonding strength of AlCrN coating are higher than that of TiAlSiN coating, while the friction coefficient of AlCrN coating is lower than that of TiAlSiN coating. Both the cutting force and cutting temperature of AlCrN coated tool are lower than TiAlSiN coated tool. The time required for the bluntness of the AlCrN coated tool is approximately 33.3% longer than that of the TiAlSiN coated tool, and the main wear mechanisms of both tools are mainly crater wear, diffusion wear and oxidation wear.