Sulforaphane protects against acetaminophen-induced hepatotoxicity

Noh, Jung-Ran,Kim, Yong-Hoon,Hwang, Jung Hwan,Choi, Dong-Hee,Kim, Kyoung-Shim,Oh, Won-Keun,Lee, Chul-Ho Elsevier 2015 Food and chemical toxicology Vol.80 No.-

<P><B>Abstract</B></P> <P>Oxidative stress is closely associated with acetaminophen (APAP)-induced toxicity. Heme oxygenase-1 (HO-1), an antioxidant defense enzyme, has been shown to protect against oxidant-induced tissue injury. This study investigated whether sulforaphane (SFN), as a HO-1 inducer, plays a protective role against APAP hepatotoxicity <I>in vitro</I> and <I>in vivo</I>. Pretreatment of primary hepatocyte with SFN induced nuclear factor E2-factor related factor (Nrf2) target gene expression, especially HO-1 mRNA and protein expression, and suppressed APAP-induced glutathione (GSH) depletion and lipid peroxidation, which eventually leads to hepatocyte cell death. A comparable effect was observed in mice treated with APAP. Mice were treated with 300 mg/kg APAP 30 min after SFN (5 mg/kg) administration and were then sacrificed after 6 h. APAP alone caused severe liver injuries as characterized by increased plasma AST and ALT levels, GSH depletion, apoptosis, and 4-hydroxynonenal (4-HNE) formations. This APAP-induced liver damage was significantly attenuated by pretreatment with SFN. Furthermore, while hepatic reactive oxygen species (ROS) levels were increased by APAP exposure, pretreatment with SFN completely blocked ROS formation. These results suggest that SFN plays a protective role against APAP-mediated hepatotoxicity through antioxidant effects mediated by HO-1 induction. SFN has preventive action in oxidative stress-mediated liver injury.</P> <P><B>Highlights</B></P> <P> <UL> <LI> SFN pretreatment increases the cell viability against APAP-induced toxicity. </LI> <LI> SFN pretreatment protects depletion of cellular GSH after APAP treatment. </LI> <LI> SFN pretreatment enhances Nrf2 target gene expression, especially HO-1 after APAP treatment. </LI> <LI> SFN has protective effect against APAP overdose-induced liver injury <I>in vivo</I> model as well. </LI> </UL> </P>

위암종과 Epstein-Barr 바이러스와의 연관성에 대한 연구

심광용,김호영,김효열,백순구,권상옥,조미연,이창훈,이종인 건국대학교 의과학연구소 2004 건국의과학학술지 Vol.14 No.-

Objectives: Epstein-Barr virus (EBV) has been known to be linked to a spectrum of neoplastic conditions, including nasopharyngeal carcinoma, Burkitt's lymphoma, peripheral T-cell lymphoma and Hodgkin's disease. This study aims to investigate the association of EBV with gastric carcinoma in Korea. Methods: Fifty-three cases of gastric adenocarcinoma were studied for evidence of EBV infection by EBV-encoded small RNA (EBER) in situ hybridization (ISH) on the paraffin sections and amplifying the EBV genome encoding envelop glycoprotein (gp220) with polymerase chain reaction (PCR) in fresh gastric cancer specimens. Results: EBER was detected in 7 (13.2%) of 53 gastric carcinomas and all cases were male. In 6 (85.7%) of 7 EBER-positive cases, the tumors were located in the upper and middle part of the stomach. EBER ISH study showed strong positivity in all the tumor cells, but negativity in surrouning lymphocytes, stromal cells and normal gastric mucosa. DNA PCR was positive in 23 (43.4%) of 53 gastric carcinomas, including all of 7 EBER-positive cases. Conclusion: We could observe some association of EBV with gastric carcinoma, and our findings about the charateristics of EBV-positive gastric cancers involving sex, site and histological type are similar to those in Japan. However, EBV-positive rate of gastric cancer is higher than in Japan and lower than in Western countries. Further studies to elucidate oncogenic mechanism of EBV in gastric cancer should be performed.

도산지수를 이용한 부실예측에 관한 연구

심갑용,정호섭,조경선 전북대학교 산업경제연구소 1999 전북대학교 산업경제연구소 논문집 Vol.30 No.-

어떤이 일이 일어나기 전에 그것을 예측하는 것은 그것이 어떤 일이든지 그리 간단하지는 않다. 특히 지진예측은 불가능에 가까울 정도로 어려운 일이라고들 한다. 그에 비해 도산을 사전에 예측하는 것은 훨씬 쉽다. 왜냐하면 지진은 지구의 지각구조가 갑자기 일으키는 현상으로서 그 구조가 충분히 해명되어 있지 않은데 비해, 기업의 도산은 사람이 기업활동을 통해 일어나는 결과로서 그 메커니즘을 상당히 정확하게 파악할 수 있기 때문이다. 기업의 도산은 개인행동이나 기업행동으로서 감지 할 수 있으며 소문이나 기업의 분위기도 기업의 부실이나 도산을 예측하는 중요한 판단 자료일 수 있다. 그러나 이러한 인지보다는 기업이 제공하는 재무자료를 통해서 그 기업의 경영상태나 재무상태를 알 수 있기 때문에 재무정보를 바탕으로 신뢰성있는 기업의 부실이나 도산을 예측할 수 있다. 본 연구에서 도산지수라는 새로운 개념을 도입하여 기존의 연구들이 가지고 있는 기업부실예측의 한계점에 극복하고자 한다. 이를 위해서 본 연구에서는 기존의 연구들이 사용하는 재무비율변수를 그대로 사용하지 않고 기업의 부실이나 도산의 예측에 좀 더 적합하도록 하기 위해 수정한 재무비율변수를 사용할 것이다. 따라서 기존연구에서 사용된 재무비율과 상당히 다르다. 이러한 재무변수로 통계적 기법을 사용하여 부실기업과 비부실기업간의 차이 분석을 통해 결과를 도출하고 이렇게 도출된 변수로 도산지수라는 개념을 사용하여 기업의 부실을 쉽고 용이하게 예측하고자 했다. 그 결과 연구가설에서 설정한 5가지 재무변수를 검증한 결과 부실기업과 비부실기업간의 차이가 매우 유의적으로 나타났으며, 또한 부실전 3년간의 부실기업의 도산지수가 1.0보다 상당히 작게 나타났으며 비부실기업의 도산지수는 1.0보다 크지는 않지만 1.0에 매우 근접해 있었다. 이러한 결과를 바탕으로 도산지수를 이용하여 부실기업을 예측하는데 일반화 가능성을 확인 할 수 있었다. 본 연구에서는 사례연구도 병행하여 그 적용을 타진해보았다.

정신분열병 환자 가족을 위한 한 정신교육적 가족치료 모형의 효과

이영호,심주철,이상경,서영수,김용관,김규수,김영훈 大韓神經精神醫學會 2000 신경정신의학 Vol.39 No.3

연구목적 : 본 교실에서 정신분열병 환자 가족을 대상으로 시행 중인 장기 정신교육적 가족치료모형을 소개하고, 이모형이 가족의 대처방식과 우울증상, 환자가 자각하는 가족의 정서적 지지와 우울증상, 재입원율 및 사회재활상태에 미치는 영향을 평가하였다. 방 법 : 연구대상은 DSM-IV 진단기준에 의해 정신분열병으로 진단받고 외래 통원치료를 받고 있는 환자와 그 보호자였다. 교육군은 전체 19회 교육 중 최소 ⅔이상 참여한 11명의 환자와 가족이었고 비교육군은 부산소재의 종합병원에서 가족교육을 받지 않고 통원치료만을 받았던 11명의 환자와 가족이었다. 정신교육적 가족치료 모형의 효과는 가족의 대처방식과 우울증상, 환자가 자각하는 가족의 정서적 지지와 우울증상, 재입원율, 사호재활 실태로 나누어 평가했다. 평가도구는 The Ways of Coping Checklist의 국내번안용, Family Emotional Support Questionnaire for Transaction. Beck Depression Inventory를 이용했다. 재입원율 및 사회재활 실태에 대한 평가는 환자 및 가족과의 면담을 통해 조사했다. 결 과 : 본 연구의 정신교육적 가족치료 모형 실시 후 가족의 대처방식 중 적극적 대처는 교육군운 의미있게 증가한 반면 비교육군에서는 변화가 없었다. 소극적 대처 교육군은 의미있게 감소했으나 비교육군에서는 변화가 없었다. 가족치료모형 실시 전 가족들은 양군 모두 심한 우울증상을 갖고 있었다. 가족치료모형 실시 후 가족의 우울증상은 교육군에서 감소, 비교육군은 증가를 보였으나 통계적 유의성은 없었다. 환자가 지각하는 가족의 정서적 지지 평가척도점수는 교육군은 의미있는 증가를 보였으나 비교육군에서는 변화가 없었다. 환자의 우울증상의 교육군에서는 현저한 감소를 보인 반면 비교육군은 상반되게 증가했다. 가족의 적극적 대처가 환자가 자각하는 정서적 지지의 증가 및 우울증상 감소와 의미있는 상관관계를 나타냈다. 또한 교육군의 환자들이 비교육군의 환자들보다 장기가족교육 종료시점과 9개월 경과 후의 추적 평가에서 재입원율이 현저히 낮았고, 사회재활상태도 좋았다. 결 론 : 본 연구에서 실시된 장기 가족교육모형은 가족의 대처방식을 향상시키고, 우울증상의 감소를 가져오며, 환자가 자각하는 가족의 정서적 지지 향상 및 우울증상의 호전을 가져오고, 재입원율의 저하 및 사회재활의 향상을 보이는 것으로 나타났다. Objectives : This study was performed to introduce a psychoeducational family therapy model for the families of schizophrenic patient and to investigate the effect of this model on the changes in coping style and depressive symptoms of the family members, and in perception of emotional support by families and depressive symptoms of patients. Methods : Subjects were schizophrenic out-patients, who fufilled DSM-IV diagnostic criteria, and their families. The members of "education group" were 11 families and patients who participated in more than two thirds of 19 sessions of the program. The members of "non-education group" were 11 families and patients who had no experience of family education and were treated in a general hospital in Pusan. Clinical ratings were performed using by The Ways of Coping Checklist, Family Emotional Support Questionnaire for Transactions, and Beck Depression Inventory. Results : Coping style was changed positively, in education group but not in non-education group. The depressive symptoms of families were decreased in educated group and increased in non-educated group, although there were no statistical significance. In education group, patients felt much better emotional support by their families than patients in non-education group did. At the same time, their depressive symptoms were significantly reduced. The patients from education group were on more successful rehabilitation than those from non-education group were. Conclusion : Our results suggest that this psychoeducational family therapy model would be helpful to induce the positive changes in coping styles and reduce the depressive symptoms of family members, and it could also induce the reduction of depressive symptoms of schizophrenic patients and help their rehabilitation.

WDM 시스템용 광대역 증폭기의 시뮬레이션

李鎬俊,沈雲用,鄭白鎬 湖西大學校工業技術硏究所 1999 工業技術硏究所論文集 Vol.18 No.-

In recent years, the biggest issue of WDM system is in increasing its bandwidth. Transmission bandwidth of WDM may be limited by many kinds of component, and the bandwidth plays impotent role in most of all optical amplifiers. Transmission bandwidth of recent WDM is limited in about 40 nm. This limit depends on EDFA(?? Fiber Amplifier) gain bandwidth. To overcome this problem, we may use L and S band using Yb/Nd doped fiber amplifier. Wideband amplifier, which is consisted of RFA for L band and EDFA for M band using single pump laser diode, is presented. The amplifiers have been analyzed numerically for the design.

복압성요실금(Stress urinary incontinence)과 절박성요실금(Urge incontinence)환자에서 TVT 수술 결과의 비교

이경구,이준호,권용욱,정태융,심희영,이상익 관동대학교 의과대학 의과학연구소 2005 關東醫大學術誌 Vol.9 No.1

Tension-free vaginal tape(TVT) has been widely used in the management of stress urinary incontinence(SUI), and has been applied to urge incontinence (UI). We evaluated and compared the outcomes between SUI and UI for patients who had undergone TVT. Patients were categorized into SUI(33 patients) and UI(26 patients). Before treatment, we asked about LUTSs using female bladder questionnaire. And we asked about sexual function. After treatment, the same questionnaires were repeated. During the postperative period, the objective and subjective success rate were evaluated. For operation outcome results, in SUI group there were 29 cases of cure (87.9%)and 2 of improvement(6.1%), and in UI group there were 20 cases of cure(76.9%)and 3 of improvement(11.5%) (p>0.05). The operation satisfaction rate of patients was 90.9% in SUI group and 84.6% in UI group(p>0.05). Improvement of sexual satisfaction occurred in 6(20.0%) of SUI group, and 3 (18.8%) of UI group, respectively(p>0.05). The objective success rate, satisfaction rate and sexual satisfaction of SUI patients did not significantly differ from the women with UI. Also, these results shows that TVT procedure improves some LUTSs in both groups. We consider the TVT procedure to be an effective treatment not for stress urinary incontinence but also urge incontinenece.

Protective Effect of Soybean-Derived Phosphatidylserine on the Trimethyltin-Induced Learning and Memory Deficits in Rats

Yong Ho An,Hyun Jung Park,Hyun Soo Shim,Yun Seok Choe,Jeong Jun Han,Jin Su Kim,Hye Jung Lee,Insop Shim 한의병리학회 2014 동의생리병리학회지 Vol.28 No.3

Chemical Resistant Expression of Diaporthe Citri Causing Melanose in Citrus

Yong Ho Shin,Zar Zar Soe,Hyun Jin Shim,Young Chan Kim,Seung Eon Kim,Yong Chull Jeun 한국농약과학회 2024 한국농약과학회 학술발표대회 논문집 Vol.2024 No.4

Effects of a passive exoskeleton on shoulder strain associated with augmented reality

Yong-Ku Kong(공용구),Sang-Soo Park(박상수),Jin-Woo Shim(심진우),Kyeong-Hee Choi(최경희),Hyun-Ho Shim(심현호),Kiana Kia,Jeong Ho Kim(김정호) 대한인간공학회 2022 대한인간공학회 학술대회논문집 Vol.2022 No.10

Amphotericin B Aggregation Inhibition with Novel Nanoparticles Prepared with Poly(ε-caprolactone)/Poly(N,N-dimethylamino-2-ethyl methacrylate) Diblock Copolymer

( Yong Ho Shim ),( You Chan Kim ),( Hong Joo Lee ),( Francois Bougard ),( Philippe Dubois ),( Ki Choon Choi ),( Chung Wook Chung ),( Dae Hwan Kang ),( Young Il Jeong ) 한국미생물 · 생명공학회 2011 Journal of microbiology and biotechnology Vol.21 No.1

Diblock copolymers composed of poly(ε-caprolactone) (PCL) and poly(N,N-dimethylamino-2-ethyl methacrylate) (PDMAEMA), or methoxy polyethylene glycol(PEG), were synthesized via a combination of ring-opening polymerization and atom-transfer radical polymerization in order to prepare polymeric nanoparticles as an antifungal drug carrier. Amphotericin B (AmB), a natural antibiotic, was incorporated into the polymeric nanoparticles. The physical properties of AmB-incorporated polymeric nanoparticles with PCL-b-PDMAEMA and PCL-b-PEG were studied in relation to morphology and particle size. In the aggregation state study, AmB-incorporated PCL-b- PDMAEMA nanoparticles exhibited a monomeric state pattern of free AmB, whereas AmB-incorporated PCL-b- PEG nanoparticles displayed an aggregated pattern. In in vitro hemolysis tests with human red blood cells, AmB-incorporated PCL-b-PDMAEMA nanoparticles were seen to be 10 times less cytotoxic than free AmB (5 μg/ml). In addition, an improved antifungal activity of AmBincorporated polymeric nanoparticles was observed through antifungal activity tests using Candida albicans, whereas polymeric nanoparticles themselves were seen not to affect activity. Finally, in vitro AmB release studies were conducted, proving the potential of AmB-incorporated PCL-b-PDMAEMA nanoparticles as a new formulation candidate for AmB.